pLVPRT-tTR-KRAB

LOCUS       pLVPRT-tTR-KRAB	11583 bp 	DNA	SYN
DEFINITION  pLVPRT-tTR-KRAB
ACCESSION   
KEYWORDS    
SOURCE      
  ORGANISM  other sequences; artificial sequences; vectors.
FEATURES             Location/Qualifiers
     source          1..11583
                     /organism="pLVPRT-tTR-KRAB"
                     /mol_type="other DNA"
     misc_feature    461..641
                     /label="truncHIV-1_3_LTR"
     misc_feature    461..641
                     /label="HIV-1_5_LTR"
     misc_feature    752..796
                     /label="HIV-1_psi_pack"
     CDS             1184..1684
                     /label="ORF frame 2"
     misc_feature    1306..1539
                     /label="RRE"
     misc_feature    2359..2634
                     /label="synth_int"
     CDS             2723..3472
                     /label="ORF frame 2"
     gene            2726..3439
                     /label="EGFP"
                     /gene="EGFP"
     misc_feature    complement(2768..2789)
                     /label="EGFP_N_primer"
     misc_feature    2906..2935
                     /label="Y66 (EGFP)"
     misc_feature    3376..3397
                     /label="EGFP_C_primer"
     misc_feature    3523..4085
                     /label="IRES"
     CDS             4089..5096
                     /label="ORF frame 3"
     misc_feature    4104..4703
                     /label="TetR"
     misc_feature    4710..4730
                     /label="SV40 NLS"
     misc_feature    4716..4730
                     /label="NLS"
     misc_feature    5171..5758
                     /label="WPRE"
     CDS             complement(5256..5759)
                     /label="ORF frame 2"
     CDS             5259..5819
                     /label="ORF frame 3"
     misc_feature    5829..5844
                     /label="cPPT"
     misc_feature    5829..5850
                     /label="U3PPT"
     misc_feature    5881..6168
                     /label="TRE"
     misc_feature    6204..6384
                     /label="HIV-1_5_LTR"
     misc_feature    6204..6384
                     /label="truncHIV-1_3_LTR"
     misc_feature    complement(6410..6427)
                     /label="Sp6_primer"
     gene            6665..6928
                     /label="tet (300 - 563)"
                     /gene="tet (300 - 563)"
     misc_feature    complement(6717..6736)
                     /label="pBRrevBam_primer"
     misc_feature    7069..7091
                     /label="pGEX_3_primer"
     promoter        7250..7278
                     /label="AmpR_promoter"
     gene            7320..8180
                     /label="Ampicillin"
                     /gene="Ampicillin"
     CDS             7320..8180
                     /label="ORF frame 3"
     rep_origin      8335..8954
                     /label="pBR322_origin"
     misc_feature    complement(9180..9200)
                     /label="pBABE_3_primer"
     misc_feature    complement(9186..9639)
                     /label="SV40_enhancer"
     promoter        9198..9466
                     /label="SV40_promoter"
     rep_origin      9365..9442
                     /label="SV40_origin"
     misc_feature    9427..9446
                     /label="SV40pro_F_primer"
     misc_feature    10647..10662
                     /label="SV40_int"
     misc_feature    10668..10715
                     /label="SV40_3_splice"
     CDS             10792..11265
                     /label="ORF frame 1"
     misc_feature    10843..10863
                     /label="SV40 NLS"
     misc_feature    10849..10863
                     /label="NLS"
     terminator      11291..11422
                     /label="SV40_PA_terminator"
     misc_feature    11379..11398
                     /label="EBV_rev_primer"
ORIGIN
    1 tagtagttgg aagggctaat tcactcccaa agaagacaag atatccttga tctgtggatc
   61 taccacacac aaggctactt ccctgattag cagaactaca caccagggcc aggggtcaga
  121 tatccactga cctttggatg gtgctacaag ctagtaccag ttgagccaga taaggtagaa
  181 gaggccaata aaggagagaa caccagcttg ttacaccctg tgagcctgca tgggatggat
  241 gacccggaga gagaagtgtt agagtggagg tttgacagcc gcctagcatt tcatcacgtg
  301 gcccgagagc tgcatccgga gtacttcaag aactgctgat atcgagcttg ctacaaggga
  361 ctttccgctg gggactttcc agggaggcgt ggcctgggcg ggactgggga gtggcgagcc
  421 ctcagatcct gcatataagc agctgctttt tgcctgtact gggtctctct ggttagacca
  481 gatctgagcc tgggagctct ctggctaact agggaaccca ctgcttaagc ctcaataaag
  541 cttgccttga gtgcttcaag tagtgtgtgc ccgtctgttg tgtgactctg gtaactagag
  601 atccctcaga cccttttagt cagtgtggaa aatctctagc agtggcgccc gaacagggac
  661 ttgaaagcga aagggaaacc agaggagctc tctcgacgca ggactcggct tgctgaagcg
  721 cgcacggcaa gaggcgaggg gcggcgactg gtgagtacgc caaaaatttt gactagcgga
  781 ggctagaagg agagagatgg gtgcgagagc gtcagtatta agcgggggag aattagatcg
  841 cgatgggaaa aaattcggtt aaggccaggg ggaaagaaaa aatataaatt aaaacatata
  901 gtatgggcaa gcagggagct agaacgattc gcagttaatc ctggcctgtt agaaacatca
  961 gaaggctgta gacaaatact gggacagcta caaccatccc ttcagacagg atcagaagaa
 1021 cttagatcat tatataatac agtagcaacc ctctattgtg tgcatcaaag gatagagata
 1081 aaagacacca aggaagcttt agacaagata gaggaagagc aaaacaaaag taagaccacc
 1141 gcacagcaag cggccgctga tcttcagacc tggaggagga gatatgaggg acaattggag
 1201 aagtgaatta tataaatata aagtagtaaa aattgaacca ttaggagtag cacccaccaa
 1261 ggcaaagaga agagtggtgc agagagaaaa aagagcagtg ggaataggag ctttgttcct
 1321 tgggttcttg ggagcagcag gaagcactat gggcgcagcc tcaatgacgc tgacggtaca
 1381 ggccagacaa ttattgtctg gtatagtgca gcagcagaac aatttgctga gggctattga
 1441 ggcgcaacag catctgttgc aactcacagt ctggggcatc aagcagctcc aggcaagaat
 1501 cctggctgtg gaaagatacc taaaggatca acagctcctg gggatttggg gttgctctgg
 1561 aaaactcatt tgcaccactg ctgtgccttg gaatgctagt tggagtaata aatctctgga
 1621 acagattgga atcacacgac ctggatggag tgggacagag aaattaacaa ttacacaagc
 1681 ttaatacact ccttaattga agaatcgcaa aaccagcaag aaaagaatga acaagaatta
 1741 ttggaattag ataaatgggc aagtttgtgg aattggttta acataacaaa ttggctgtgg
 1801 tatataaaat tattcataat gatagtagga ggcttggtag gtttaagaat agtttttgct
 1861 gtactttcta tagtgaatag agttaggcag ggatattcac cattatcgtt tcagacccac
 1921 ctcccaaccc cgaggggacc cgacaggccc gaaggaatag aagaagaagg tggagagaga
 1981 gacagagaca gattcgagat ctttacagtc aaatgagcca cgtcagggag cgatggcacc
 2041 cgcaggcggt atcaactgat gcaagtgttc aagcgaatct caactcgttt tttccggtga
 2101 ctcattcccg gccctggttg gcagcgctgc accctttaac ttaaacctcg gccggccgcc
 2161 cgccgggggc acagagtgtg cgccgggccg cgcggcaatt ggtccccgcg ccgacctccg
 2221 cccgcgagcg ccgccgcttc ccttccccgc cccgcgtccc tccccctcgg ccccgcgcgt
 2281 cgcctgtcct ccgagccagt cgctgacagc cgcggcgccg cgagcttctc ctctcctcac
 2341 gaagatccta gaattcgctg tctgcgaggg ccagctgttg gggtgagtac tccctctcaa
 2401 aagcgggcat gacttctgcg ctaagattgt cagtttccaa aaacgaggag gatttgatat
 2461 tcacctggcc cgcggtgatg cctttgaggg tggccgcgtc catctggtca gaaaagacaa
 2521 tctttttgtt gtcaagcttg aggtgtggca ggcttgagat ctggccatac acttgagtga
 2581 caatgacatc cactttgcct ttctctccac aggtgtccac tcccaggtcc aactgcggac
 2641 tttcgatatc aagcttatcg atacccgtcg acctcgagat ccaggcctaa gcttacgcgt
 2701 cctagcgcta ccggtcgcca ccatggtgag caagggcgag gagctgttca ccggggtggt
 2761 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
 2821 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
 2881 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
 2941 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
 3001 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
 3061 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
 3121 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
 3181 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
 3241 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
 3301 cgtgctgctg cccgacaacc actacctgag cacccagtcc gccctgagca aagaccccaa
 3361 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
 3421 catggacgag ctgtacaagt ccggactcag atctcgacta gctagtagct agctagctag
 3481 tcgagctcaa gcttcgaatt cccgggctgc aggaattccg cccccccccc cctaacgtta
 3541 ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta ttttccacca
 3601 tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca
 3661 ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg
 3721 aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc
 3781 agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt gtataagata
 3841 cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt gtggaaagag
 3901 tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag aaggtacccc
 3961 attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt
 4021 taaaaaacgt ctaggccccc cgaaccacgg ggacgtggtt ttcctttgaa aaacacgatg
 4081 ataataccat ggctagatta gataaaagta aagtgattaa cagcgcatta gagctgctta
 4141 atgaggtcgg aatcgaaggt ttaacaaccc gtaaactcgc ccagaagcta ggtgtagagc
 4201 agcctacatt gtattggcat gtaaaaaata agcgggcttt gctcgacgcc ttagccattg
 4261 agatgttaga taggcaccat actcactttt gccctttaga aggggaaagc tggcaagatt
 4321 ttttacgtaa taacgctaaa agttttagat gtgctttact aagtcatcgc gatggagcaa
 4381 aagtacattt aggtacacgg cctacagaaa aacagtatga aactctcgaa aatcaattag
 4441 cctttttatg ccaacaaggt ttttcactag agaatgcatt atatgcactc agcgctgtgg
 4501 ggcattttac tttaggttgc gtattggaag atcaagagca tcaagtcgct aaagaagaaa
 4561 gggaaacacc tactactgat agtatgccgc cattattacg acaagctatc gaattatttg
 4621 atcaccaagg tgcagagcca gccttcttat tcggccttga attgatcata tgcggattag
 4681 aaaaacaact taaatgtgaa agtgggtcgc caaaaaagaa gagaaaggtc gacggcggtg
 4741 gtgctttgtc tcctcagcac tctgctgtca ctcaaggaag tatcatcaag aacaaggagg
 4801 gcatggatgc taagtcacta actgcctggt cccggacact ggtgaccttc aaggatgtat
 4861 ttgtggactt caccagggag gagtggaagc tgctggacac tgctcagcag atcgtgtaca
 4921 gaaatgtgat gctggagaac tataagaacc tggtttcctt gggttatcag cttactaagc
 4981 cagatgtgat cctccggttg gagaagggag aagagccctg gctggtggag agagaaattc
 5041 accaagagac ccatcctgat tcagagactg catttgaaat caaatcatca gtttaaggat
 5101 ctcgactagc tagtagctag ctagctagtc gagctcaact tcgaattcga tgggactagt
 5161 catatgataa tcaacctctg gattacaaaa tttgtgaaag attgactggt attcttaact
 5221 atgttgctcc ttttacgcta tgtggatacg ctgctttaat gcctttgtat catgctattg
 5281 cttcccgtat ggctttcatt ttctcctcct tgtataaatc ctggttgctg tctctttatg
 5341 aggagttgtg gcccgttgtc aggcaacgtg gcgtggtgtg cactgtgttt gctgacgcaa
 5401 cccccactgg ttggggcatt gccaccacct gtcagctcct ttccgggact ttcgctttcc
 5461 ccctccctat tgccacggcg gaactcatcg ccgcctgcct tgcccgctgc tggacagggg
 5521 ctcggctgtt gggcactgac aattccgtgg tgttgtcggg gaagctgacg tcctttccat
 5581 ggctgctcgc ctgtgttgcc acctggattc tgcgcgggac gtccttctgc tacgtccctt
 5641 cggccctcaa tccagcggac cttccttccc gcggcctgct gccggctctg cggcctcttc
 5701 cgcgtcttcg ccttcgccct cagacgagtc ggatctccct ttgggccgcc tccccgcatc
 5761 ggtacgtatg gccaggtacc tttaagacca atgacttaca aggcagctgt agatcttagc
 5821 cactttttaa aagaaaaggg gggactggaa gggctaattc actcccaacg aagacaagat
 5881 ctcgagttta ccactcccta tcagtgatag agaaaagtga aagtcgagtt taccactccc
 5941 tatcagtgat agagaaaagt gaaagtcgag tttaccactc cctatcagtg atagagaaaa
 6001 gtgaaagtcg agtttaccac tccctatcag tgatagagaa aagtgaaagt cgagtttacc
 6061 actccctatc agtgatagag aaaagtgaaa gtcgagttta ccactcccta tcagtgatag
 6121 agaaaagtga aagtcgagtt taccactccc tatcagtgat agagaaaagt gaaagtcgga
 6181 tcgatctgct ttttgcttgt actgggtctc tctggttaga ccagatctga gcctgggagc
 6241 tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct tgagtgcttc
 6301 aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc agaccctttt
 6361 agtcagtgtg gaaaatctct agcagcatct agaattaatt ccgtgtattc tatagtgtca
 6421 cctaaatcgt atgtgtatga tacataaggt tatgtattaa ttgtagccgc gttctaacga
 6481 caatatgtac aagcctaatt gtgtagcatc tggcttactg aagcagaccc tatcatctct
 6541 ctcgtaaact gccgtcagag tcggtttggt tggacgaacc ttctgagttt ctggtaacgc
 6601 cgtcccgcac ccggaaatgg tcagcgaacc aatcagcagg gtcatcgcta gccagatcct
 6661 ctacgccgga cgcatcgtgg ccggcatcac cggcgccaca ggtgcggttg ctggcgccta
 6721 tatcgccgac atcaccgatg gggaagatcg ggctcgccac ttcgggctca tgagcgcttg
 6781 tttcggcgtg ggtatggtgg caggccccgt ggccggggga ctgttgggcg ccatctcctt
 6841 gcatgcacca ttccttgcgg cggcggtgct caacggcctc aacctactac tgggctgctt
 6901 cctaatgcag gagtcgcata agggagagcg tcgaatggtg cactctcagt acaatctgct
 6961 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
 7021 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
 7081 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
 7141 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
 7201 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
 7261 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
 7321 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
 7381 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
 7441 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
 7501 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
 7561 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
 7621 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
 7681 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
 7741 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
 7801 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
 7861 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
 7921 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
 7981 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
 8041 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
 8101 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
 8161 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
 8221 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
 8281 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
 8341 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
 8401 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
 8461 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
 8521 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
 8581 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
 8641 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
 8701 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gcattgagaa agcgccacgc
 8761 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
 8821 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
 8881 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
 8941 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
 9001 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
 9061 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
 9121 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctgtg
 9181 gaatgtgtgt cagttagggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
 9241 aagcatgcat ctcaattagt cagcaaccag gtgtggaaag tccccaggct ccccagcagg
 9301 cagaagtatg caaagcatgc atctcaatta gtcagcaacc atagtcccgc ccctaactcc
 9361 gcccatcccg cccctaactc cgcccagttc cgcccattct ccgccccatg gctgactaat
 9421 tttttttatt tatgcagagg ccgaggccgc ctcggcctct gagctattcc agaagtagtg
 9481 aggaggcttt tttggaggcc taggcttttg caaaaagctt ggacacaaga caggcttgcg
 9541 agatatgttt gagaatacca ctttatcccg cgtcagggag aggcagtgcg taaaaagacg
 9601 cggactcatg tgaaatactg gtttttagtg cgccagatct ctataatctc gcgcaaccta
 9661 ttttcccctc gaacactttt taagccgtag ataaacaggc tgggacactt cacatgagcg
 9721 aaaaatacat cgtcacctgg gacatgttgc agatccatgc acgtaaactc gcaagccgac
 9781 tgatgccttc tgaacaatgg aaaggcatta ttgccgtaag ccgtggcggt ctgtaccggg
 9841 tgcgttactg gcgcgtgaac tgggtattcg tcatgtcgat accgtttgta tttccagcta
 9901 cgatcacgac aaccagcgcg agcttaaagt gctgaaacgc gcagaaggcg atggcgaagg
 9961 cttcatcgtt attgatgacc tggtggatac cggtggtact gcggttgcga ttcgtgaaat
10021 gtatccaaaa gcgcactttg tcaccatctt cgcaaaaccg gctggtcgtc cgctggttga
10081 tgactatgtt gttgatatcc cgcaagatac ctggattgaa cagccgtggg atatgggcgt
10141 cgtattcgtc ccgccaatct ccggtcgcta atcttttcaa cgcctggcac tgccgggcgt
10201 tgttcttttt aacttcaggc gggttacaat agtttccagt aagtattctg gaggctgcat
10261 ccatgacaca ggcaaacctg agcgaaaccc tgttcaaacc ccgctttaaa catcctgaaa
10321 cctcgacgct agtccgccgc tttaatcacg gcgcacaacc gcctgtgcag tcggcccttg
10381 atggtaaaac catccctcac tggtatcgca tgattaaccg tctgatgtgg atctggcgcg
10441 gcattgaccc acgcgaaatc ctcgacgtcc aggcacgtat tgtgatgagc gatgccgaac
10501 gtaccgacga tgatttatac gatacggtga ttggctaccg tggcggcaac tggatttatg
10561 agtgggcccc ggatctttgt gaaggaacct tacttctgtg gtgtgacata attggacaaa
10621 ctacctacag agatttaaag ctctaaggta aatataaaat ttttaagtgt ataatgtgtt
10681 aaactactga ttctaattgt ttgtgtattt tagattccaa cctatggaac tgatgaatgg
10741 gagcagtggt ggaatgcctt taatgaggaa aacctgtttt gctcagaaga aatgccatct
10801 agtgatgatg aggctactgc tgactctcaa cattctactc ctccaaaaaa gaagagaaag
10861 gtagaagacc ccaaggactt tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt
10921 agtaatagaa ctcttgcttg ctttgctatt tacaccacaa aggaaaaagc tgcactgcta
10981 tacaagaaaa ttatggaaaa atattctgta acctttataa gtaggcataa cagttataat
11041 cataacatac tgttttttct tactccacac aggcatagag tgtctgctat taataactat
11101 gctcaaaaat tgtgtacctt tagcttttta atttgtaaag gggttaataa ggaatatttg
11161 atgtatagtg ccttgactag agatcataat cagccatacc acatttgtag aggttttact
11221 tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
11281 tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
11341 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
11401 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
11461 tcccacccca taccctatta ccactgccaa ttacctagtg gtttcattta ctctaaacct
11521 gtgattcctc tgaattattt tcattttaaa gaaattgtat ttgttaaata tgtactacaa
11581 act
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