pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BTAF1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-BTAF1-FLAG         6286 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6286)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6286
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..2990
                     /codon_start=1
                     /gene="BTAF1"
                     /gene_synonym="MOT1; TAF(II)170; TAF172; TAFII170"
                     /product="TATA-binding protein-associated factor 172"
                     /label=BTAF1
                     /note="TBP-associated factor 172; B-TFIID transcription 
                     factor-associated 170 kDa subunit; ATP-dependent helicase 
                     BTAF1; BTAF1 RNA polymerase II, B-TFIID transcription 
                     factor-associated, 170kDa (Mot1 homolog, S. cerevisiae)"
                     /db_xref="GI:27477070"
                     /db_xref="CCDS:CCDS7419.1"
                     /db_xref="GeneID:9044"
                     /db_xref="HGNC:HGNC:17307"
                     /db_xref="MIM:605191"
                     /protein_id="NP_003963.1"
                     /translation="LQHQVKRHNLIVASYDVVRNDIDFFRNIKFNYCILDEGHVIKNGK
                     TKLSKAVKQLTANYRIILSGTPIQNNVLELWSLFDFLMPGFLGTERQFAARYGKPILAS
                     RDARSSSREQEAGVLAMDALHRQVLPFLLRRMKEDVLQDLPPKIIQDYYCTLSPLQVQL
                     YEDFAKSRAKCDVDETVSSATLSEETEKPKLKATGHVFQALQYLRKLCNHPALVLTPQH
                     PEFKTTAEKLAVQNSSLHDIQHAPKLSALKQLLLDCGLGNGSTSESGTESVVAQHRILI
                     FCQLKSMLDIVEHDLLKPHLPSVTYLRLDGSIPPGQRHSIVSRFNNDPSIDVLLLTTHV
                     GGLGLNLTGADTVVFVEHDWNPMRDLQAMDRAHRIGQKRVVNVYRLITRGTLEEKIMGL
                     QKFKMNIANTVISQENSSLQSMGTDQLLDLFTLDKDGKAEKADTSTSGKASMKSILENL
                     SDLWDQEQYDSEYSLENFMHSLK"
     misc_feature    2487..2492
                     /label=SDS
     CDS             2994..3059
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3075..3506
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3219..3224
                     /label=SDS
     repeat_region   3549..3689
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3549..3678
                     /label=AAV2 ITR
     repeat_region   3550..3690
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3764..4219
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4501..4605
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4606..5466
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5637..6225
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtta cagcaccaag
     1561 taaaaaggca caatctaata gtggcttcat atgatgttgt gaggaatgac atagatttct
     1621 ttagaaatat taaatttaac tactgcattc ttgatgaagg ccatgtcatc aaaaatggaa
     1681 aaacaaagtt gtcaaaagca gtaaaacaac tgactgctaa ttataggatt attctttctg
     1741 gaacaccaat ccagaacaac gttttggagc tgtggtcatt atttgatttc ctcatgccag
     1801 gatttttggg tactgaacgc cagtttgctg ctcgatatgg taaacctata ttagcaagta
     1861 gggatgctcg aagctccagt cgagagcaag aagcaggtgt tcttgctatg gatgcgctgc
     1921 accgccaagt actaccgttt cttttgagaa gaatgaaaga agatgttttg caggatcttc
     1981 cacctaaaat tattcaagac tattattgta ctcttagtcc tctccaggtt cagctctatg
     2041 aagattttgc taagtctcgt gccaagtgtg atgttgatga aacagtttct tcagctacac
     2101 tttctgaaga aactgaaaaa ccaaagctta aagctacagg ccacgtattc caggcattac
     2161 agtacttacg taaactgtgc aaccatccag cattagtctt aacacctcaa catccagaat
     2221 tcaagaccac tgccgaaaaa ctggcagttc agaattcttc tctacatgat attcaacatg
     2281 cccctaagct ctcagctttg aaacaattgc tgttggactg cggtttggga aatggcagca
     2341 cttccgagag tggcacagag tctgttgtgg cccagcacag gatactgata ttctgtcagc
     2401 tgaaaagcat gcttgatata gtagagcatg atctcctcaa acctcacttg ccctctgtca
     2461 cttatttgag attagatggc agcatacctc ctggtcagag gcattccatt gtttcccggt
     2521 ttaataatga tccatctata gacgttctgt tacttaccac tcacgttggt ggcctgggac
     2581 ttaatttgac aggcgctgac acagtagtat ttgtggagca tgactggaat cctatgcgag
     2641 atctacaagc catggaccgg gcccatcgca ttgggcagaa acgtgtggtt aacgtatacc
     2701 gattgataac cagaggaaca ttggaagaaa aaataatggg gttgcagaaa ttcaagatga
     2761 acatagcgaa tactgttatt agccaagaga attctagttt gcagagcatg gggactgatc
     2821 agcttcttga tctgtttact cttgataagg atggcaaagc agaaaaagct gacacctcta
     2881 cttctgggaa agcaagtatg aaatcaattc ttgaaaacct gagtgatctt tgggatcaag
     2941 agcagtatga ttcagagtac agcctggaaa attttatgca ttctctcaag aaggactaca
     3001 aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac gatgacaagt
     3061 aagaattctg atgactcgag ataatcaacc tctggattac aaaatttgtg aaagattgac
     3121 tggtattctt aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt
     3181 gtatcatgct attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt
     3241 agttcttgcc acggcggaac tcatcgccgc ctgccttgcc cgctgctgga caggggctcg
     3301 gctgttgggc actgacaatt ccgtggtgtt tatttgtgaa atttgtgatg ctattgcttt
     3361 atttgtaacc atctagcttt atttgtgaaa tttgtgatgc tattgcttta tttgtaacca
     3421 ttataagctg caataaacaa gttaacaaca acaattgcat tcattttatg tttcaggttc
     3481 agggggagat gtgggaggtt ttttaaagat ctggagaccg gtgtggcacg tgcggaccga
     3541 gcggccgcag gaacccctag tgatggagtt ggccactccc tctctgcgcg ctcgctcgct
     3601 cactgaggcc gggcgaccaa aggtcgcccg acgcccgggc tttgcccggg cggcctcagt
     3661 gagcgagcga gcgcgcagct gcctgcaggg gcgcctgatg cggtattttc tccttacgca
     3721 tctgtgcggt atttcacacc gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc
     3781 gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     3841 ttagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     3901 cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc
     3961 gaccccaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     4021 gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     4081 ggaacaacac tcaactctat ctcgggctat tcttttgatt tataagggat tttgccgatt
     4141 tcggtctatt ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa
     4201 atattaacgt ttacaatttt atggtgcact ctcagtacaa tctgctctga tgccgcatag
     4261 ttaagccagc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc
     4321 ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg tcagaggttt
     4381 tcaccgtcat caccgaaacg cgcgagacga aagggcctcg tgatacgcct atttttatag
     4441 gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg
     4501 cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga
     4561 caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat
     4621 ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca
     4681 gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc
     4741 gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca
     4801 atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtat tgacgccggg
     4861 caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca
     4921 gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata
     4981 accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag
     5041 ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg
     5101 gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgt agcaatggca
     5161 acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta
     5221 atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct
     5281 ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca
     5341 gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag
     5401 gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat
     5461 tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt
     5521 taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa
     5581 cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga
     5641 gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg
     5701 gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc
     5761 agagcgcaga taccaaatac tgttcttcta gtgtagccgt agttaggcca ccacttcaag
     5821 aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc
     5881 agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg
     5941 cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac
     6001 accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga
     6061 aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt
     6121 ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag
     6181 cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg
     6241 gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgt
//