| 质粒名称: | JD633 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 160393 |
| 存储实验室: | Jorge Dubcovsky |
|
载体骨架基本信息 |
|
| 载体名称: | pTRANS_250d |
| 载体出品公司: | Daniel Voytas |
| 空载体大小: | -- |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | 双运载体 |
| 筛选标记: | 潮霉素；PvUbi2:hpt-II |
| 载体抗性: | 卡那霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a |
| 拷贝数: | -- |
|
插入基因信息 |
|
| 插入基因名称: | ZmUbi::wheat GRF4-GIF1 chimera |
| 物种来源: | Synthetic |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子: | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | -- |
| 5’克隆位点: | -- |
| 3’克隆位点: | -- |
| 5’测序引物 | -- |
| 3’测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | JD633 was a gift from Jorge Dubcovsky (Addgene plasmid # 160393 ; http://n2t.net/addgene:160393 ; RRID:Addgene_160393) |
| 参考文献部分: | A GRF-GIF chimeric protein improves the regeneration efficiency of transgenic plants. Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik JF, Dubcovsky J. Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi: 10.1038/s41587-020-0703-0. 10.1038/s41587-020-0703-0 PubMed 33046875 |
买家导航
JD633载体质粒基本信息
JD633质粒图谱载体图谱和JD633载体序列质粒序列多克隆位点信息
JD633质粒载体简介
JD633质粒序列载体序列
LOCUS JD633 21667 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION CRISPR-Cas9 vector including GRF4-GIF1. For wheat and other monocots
CRISPR experiments. Includes two AarI sites for gRNA cloning.
ACCESSION .
VERSION .
KEYWORDS JD633
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 21667)
AUTHORS Debernardi JM, Tricoli DM, Ercoli MF, Hayta S, Ronald P, Palatnik
JF, Dubcovsky J
TITLE A GRF-GIF chimeric protein improves the regeneration efficiency of
transgenic plants.
JOURNAL Nat Biotechnol. 2020 Oct 12. pii: 10.1038/s41587-020-0703-0. doi:
10.1038/s41587-020-0703-0.
PUBMED 33046875
REFERENCE 2 (bases 1 to 21667)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..21667
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 1233..1862
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
CDS 2291..3364
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
rep_origin 3430..3624
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
primer_bind complement(3860..3882)
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
misc_feature 3968..4108
/label=bom
/note="basis of mobility region from pBR322"
primer_bind 4023..4042
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind complement(4123..4140)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4294..4882)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4374..4393)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(4969..5763)
/codon_start=1
/gene="aphA-3"
/product="aminoglycoside phosphotransferase"
/label=KanR
/note="confers resistance to kanamycin"
/translation="MAKMRISPELKKLIEKYRCVKDTEGMSPAKVYKLVGENENLYLKM
TDSRYKGTTYDVEREKDMMLWLEGKLPVPKVLHFERHDGWSNLLMSEADGVLCSEEYED
EQSPEKIIELYAECIRLFHSIDISDCPYTNSLDSRLAELDYLLNNDLADVDCENWEEDT
PFKDPRELYDFLKTEKPEEELVFSHGDLGDSNIFVKDGKVSGFIDLGRSGRADKWYDIA
FCVRSIREDIGEEQYVELFFDLLGIKPDWEKIKYYILLDELF"
misc_feature 6188..6212
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
polyA_signal 6290..6464
/label=CaMV poly(A) signal
/note="cauliflower mosaic virus polyadenylation signal"
CDS complement(6505..7530)
/codon_start=1
/gene="aph(4)-Ia"
/product="aminoglycoside phosphotransferase from E. coli"
/label=HygR
/note="confers resistance to hygromycin"
/translation="MKKPELTATSVEKFLIEKFDSVSDLMQLSEGEESRAFSFDVGGRG
YVLRVNSCADGFYKDRYVYRHFASAALPIPEVLDIGEFSESLTYCISRRSQGVTLQDLP
ETELPAVLQPVAEAMDAIAAADLSQTSGFGPFGPQGIGQYTTWRDFICAIADPHVYHWQ
TVMDDTVSASVAQALDELMLWAEDCPEVRHLVHADFGSNNVLTDNGRITAVIDWSEAMF
GDSQYEVANIFFWRPWLACMEQQTRYFERRHPELAGSPRLRAYMLRIGLDQLYQSLVDG
NFDDAAWAQGRCDAIVRSGAGTVGRTQIARRSAAVWTDGCVEVLADSGNRRPSTRPRAK
K"
protein_bind 9470..9491
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 9506..9536
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 9544..9560
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 9568..9584
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 11629..11653
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
regulatory 11650..11659
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
protein_bind complement(13568..13592)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 13621..13710
/codon_start=1
/product="three tandem HA epitope tags"
/label=3xHA
/translation="YPYDVPDYAGYPYDVPDYAGSYPYDVPDYA"
terminator 13744..13996
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS 16022..20125
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
DATLIHQSITGLYETRIDLSQLGGD"
CDS 20126..20173
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
CDS complement(20810..21115)
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITPSAQLTLTKGNKSWRTSRGGPVPNSPYSESYYARSLAVVL
QRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRSLNGEWDAPCSGTCL"
primer_bind 20980..20996
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 21006..21025
/label=T7
/note="T7 promoter, forward primer"
promoter 21006..21024
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind complement(21072..21092)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(21072..21090)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(21111..21127)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 21135..21151
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(21159..21189)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 21204..21225
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
misc_RNA 21264..21339
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
primer_bind complement(21356..21372)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
misc_feature 21575..21599
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
ORIGIN
1 tccatttgta tgtgcatgcc aaccacaggg ttcccctcgg gatcaaagta ctttgatcca
61 acccctccgc tgctatagtg cagtcggctt ctgacgttca gtgcagccgt cttctgaaaa
121 cgacatgtcg cacaagtcct aagttacgcg acaggctgcc gccctgccct tttcctggcg
181 ttttcttgtc gcgtgtttta gtcgcataaa gtagaatact tgcgactaga accggagaca
241 ttacgccatg aacaagagcg ccgccgctgg cctgctgggc tatgcccgcg tcagcaccga
301 cgaccaggac ttgaccaacc aacgggccga actgcacgcg gccggctgca ccaagctgtt
361 ttccgagaag atcaccggca ccaggcgcga ccgcccggag ctggccagga tgcttgacca
421 cctacgccct ggcgacgttg tgacagtgac caggctagac cgcctggccc gcagcacccg
481 cgacctactg gacattgccg agcgcatcca ggaggccggc gcgggcctgc gtagcctggc
541 agagccgtgg gccgacacca ccacgccggc cggccgcatg gtgttgaccg tgttcgccgg
601 cattgccgag ttcgagcgtt ccctaatcat cgaccgcacc cggagcgggc gcgaggccgc
661 caaggcccga ggcgtgaagt ttggcccccg ccctaccctc accccggcac agatcgcgca
721 cgcccgcgag ctgatcgacc aggaaggccg caccgtgaaa gaggcggctg cactgcttgg
781 cgtgcatcgc tcgaccctgt accgcgcact tgagcgcagc gaggaagtga cgcccaccga
841 ggccaggcgg cgcggtgcct tccgtgagga cgcattgacc gaggccgacg ccctggcggc
901 cgccgagaat gaacgccaag aggaacaagc atgaaaccgc accaggacgg ccaggacgaa
961 ccgtttttca ttaccgaaga gatcgaggcg gagatgatcg cggccgggta cgtgttcgag
1021 ccgcccgcgc acggctcaac cgtgcggctg catgaaatcc tggccggttt gtctgatgcc
1081 aagctggcgg cctggccggc cagcttggcc gctgaagaaa ccgagcgccg ccgtctaaaa
1141 aggtgatgtg tatttgagta aaacagcttg cgtcatgcgg tcgctgcgta tatgatgcga
1201 tgagtaaata aacaaatacg caaggggaac gcatgaaggt tatcgctgta cttaaccaga
1261 aaggcgggtc aggcaagacg accatcgcaa cccatctagc ccgcgccctg caactcgccg
1321 gggccgatgt tctgttagtc gattccgatc cccagggcag tgcccgcgat tgggcggccg
1381 tgcgggaaga tcaaccgcta accgttgtcg gcatcgaccg cccgacgatt gaccgcgacg
1441 tgaaggccat cggccggcgc gacttcgtag tgatcgacgg agcgccccag gcggcggact
1501 tggctgtgtc cgcgatcaag gcagccgact tcgtgctgat tccggtgcag ccaagccctt
1561 acgacatatg ggccaccgcc gacctggtgg agctggttaa gcagcgcatt gaggtcacgg
1621 atggaaggct acaagcggcc tttgtcgtgt cgcgggcgat caaaggcacg cgcatcggcg
1681 gtgaggttgc cgaggcgctg gccgggtacg agctgcccat tcttgagtcc cgtatcacgc
1741 agcgcgtgag ctacccaggc actgccgccg ccggcacaac cgttcttgaa tcagaacccg
1801 agggcgacgc tgcccgcgag gtccaggcgc tggccgctga aattaaatca aaactcattt
1861 gagttaatga ggtaaagaga aaatgagcaa aagcacaaac acgctaagtg ccggccgtcc
1921 gagcgcacgc agcagcaagg ctgcaacgtt ggccagcctg gcagacacgc cagccatgaa
1981 gcgggtcaac tttcagttgc cggcggagga tcacaccaag ctgaagatgt acgcggtacg
2041 ccaaggcaag accattaccg agctgctatc tgaatacatc gcgcagctac cagagtaaat
2101 gagcaaatga ataaatgagt agatgaattt tagcggctaa aggaggcggc atggaaaatc
2161 aagaacaacc aggcaccgac gccgtggaat gccccatgtg tggaggaacg ggcggttggc
2221 caggcgtaag cggctgggtt gcctgccggc cctgcaatgg cactggaacc cccaagcccg
2281 aggaatcggc gtgagcggtc gcaaaccatc cggcccggta caaatcggcg cggcgctggg
2341 tgatgacctg gtggagaagt tgaaggccgc gcaggccgcc cagcggcaac gcatcgaggc
2401 agaagcacgc cccggtgaat cgtggcaagc ggccgctgat cgaatccgca aagaatcccg
2461 gcaaccgccg gcagccggtg cgccgtcgat taggaagccg cccaagggcg acgagcaacc
2521 agattttttc gttccgatgc tctatgacgt gggcacccgc gatagtcgca gcatcatgga
2581 cgtggccgtt ttccgtctgt cgaagcgtga ccgacgagct ggcgaggtga tccgctacga
2641 gcttccagac gggcacgtag aggtttccgc agggccggcc ggcatggcca gtgtgtggga
2701 ttacgacctg gtactgatgg cggtttccca tctaaccgaa tccatgaacc gataccggga
2761 agggaaggga gacaagcccg gccgcgtgtt ccgtccacac gttgcggacg tactcaagtt
2821 ctgccggcga gccgatggcg gaaagcagaa agacgacctg gtagaaacct gcattcggtt
2881 aaacaccacg cacgttgcca tgcagcgtac gaagaaggcc aagaacggcc gcctggtgac
2941 ggtatccgag ggtgaagcct tgattagccg ctacaagatc gtaaagagcg aaaccgggcg
3001 gccggagtac atcgagatcg agctagctga ttggatgtac cgcgagatca cagaaggcaa
3061 gaacccggac gtgctgacgg ttcaccccga ttactttttg atcgatcccg gcatcggccg
3121 ttttctctac cgcctggcac gccgcgccgc aggcaaggca gaagccagat ggttgttcaa
3181 gacgatctac gaacgcagtg gcagcgccgg agagttcaag aagttctgtt tcaccgtgcg
3241 caagctgatc gggtcaaatg acctgccgga gtacgatttg aaggaggagg cggggcaggc
3301 tggcccgatc ctagtcatgc gctaccgcaa cctgatcgag ggcgaagcat ccgccggttc
3361 ctaatgtacg gagcagatgc tagggcaaat tgccctagca ggggaaaaag gtcgaaaagg
3421 cctctttcct gtggatagca cgtacattgg gaacccaaag ccgtacattg ggaaccggaa
3481 cccgtacatt gggaacccaa agccgtacat tgggaaccgg tcacacatgt aagtgactga
3541 tataaaagag aaaaaaggcg atttttccgc ctaaaactct ttaaaactta ttaaaactct
3601 taaaacccgc ctggcctgtg cataactgtc tggccagcgc acagcccaag agctgcaaaa
3661 agcgcctacc cttcggtcgc tgcgctccct acgccccgcc gcttcgcgtc ggcctatcgc
3721 ggccgctggc cgctcaaaaa tggctggcct acggccaggc aatctaccag ggcgcggaca
3781 agccgcgccg tcgccactcg accgccggcg cccacatcaa ggcaccctgc ctcgcgcgtt
3841 tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggaaacggtc acagcttgtc
3901 tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt gttggcgggt
3961 gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact ggcttaacta
4021 tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag
4081 atgcgtaagg agaaaatacc gcatcaggcc ctcttccgct tcctcgctca ctgactcgct
4141 gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt
4201 atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc
4261 caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga
4321 gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata
4381 ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac
4441 cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata gctcacgctg
4501 taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc
4561 cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag
4621 acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt
4681 aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt
4741 atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg
4801 atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac
4861 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
4921 gtggaacgaa aactcacgtt aagggatttt ggtcatgcat tctaggtact aaaacaattc
4981 atccagtaaa atataatatt ttattttctc ccaatcaggc ttgatcccca gtaagtcaaa
5041 aaatagctcg acatactgtt cttccccgat atcctccctg atcgaccgga cgcagaaggc
5101 aatgtcatac cacttgtccg ccctgccgct tctcccaaga tcaataaagc cacttacttt
5161 gccatctttc acaaagatgt tgctgtctcc caggtcgccg tgggaaaaga caagttcctc
5221 ttcgggcttt tccgtcttta aaaaatcata cagctcgcgc ggatctttaa atggagtgtc
5281 ttcttcccag ttttcgcaat ccacatcggc cagatcgtta ttcagtaagt aatccaattc
5341 ggctaagcgg ctgtctaagc tattcgtata gggacaatcc gatatgtcga tggagtgaaa
5401 gagcctgatg cactccgcat acagctcgat aatcttttca gggctttgtt catcttcata
5461 ctcttccgag caaaggacgc catcggcctc actcatgagc agattgctcc agccatcatg
5521 ccgttcaaag tgcaggacct ttggaacagg cagctttcct tccagccata gcatcatgtc
5581 cttttcccgt tccacatcat aggtggtccc tttataccgg ctgtccgtca tttttaaata
5641 taggttttca ttttctccca ccagcttata taccttagca ggagacattc cttccgtatc
5701 ttttacgcag cggtattttt cgatcagttt tttcaattcc ggtgatattc tcattttagc
5761 catttattat ttccttcctc ttttctacag tatttaaaga taccccaaga agctaattat
5821 aacaagacga actccaattc actgttcctt gcattctaaa accttaaata ccagaaaaca
5881 gctttttcaa agttgttttc aaagttggcg tataacatag tatcgacgga gccgattttg
5941 aaaccgcggt gatcacaggc agcaacgctc tgtcatcgtt acaatcaaca tgctaccctc
6001 cgcgagatca tccgtgtttc aaacccggca gcttagttgc cgttcttccg aatagcatcg
6061 gtaacatgag caaagtctgc cgccttacaa cggctctccc gctgacgccg tcccggactg
6121 atgggctgcc tgtatcgagt ggtgattttg tgccgagctg ccggtcgggg agctgttggc
6181 tggctggtgg caggatatat tgtggtgtaa acaaattgac gcttagacaa cttaataaca
6241 cattgcggac gtttttaatg tactgaatta acgccgaatt aattcggggg atctggattt
6301 tagtactgga ttttggtttt aggaattaga aattttattg atagaagtat tttacaaata
6361 caaatacata ctaagggttt cttatatgct caacacatga gcgaaaccct ataggaaccc
6421 taattccctt atctgggaac tactcacaca ttattatgga gaaactcgag cttgtcgatc
6481 gacagatccc ggtcggcatc tactctattt ctttgccctc ggacgagtgc tggggcgtcg
6541 gtttccacta tcggcgagta cttctacaca gccatcggtc cagacggccg cgcttctgcg
6601 ggcgatttgt gtacgcccga cagtcccggc tccggatcgg acgattgcgt cgcatcgacc
6661 ctgcgcccaa gctgcatcat cgaaattgcc gtcaaccaag ctctgataga gttggtcaag
6721 accaatgcgg agcatatacg cccggagtcg tggcgatcct gcaagctccg gatgcctccg
6781 ctcgaagtag cgcgtctgct gctccataca agccaaccac ggcctccaga agaagatgtt
6841 ggcgacctcg tattgggaat ccccgaacat cgcctcgctc cagtcaatga ccgctgttat
6901 gcggccattg tccgtcagga cattgttgga gccgaaatcc gcgtgcacga ggtgccggac
6961 ttcggggcag tcctcggccc aaagcatcag ctcatcgaga gcctgcgcga cggacgcact
7021 gacggtgtcg tccatcacag tttgccagtg atacacatgg ggatcagcaa tcgcgcatat
7081 gaaatcacgc catgtagtgt attgaccgat tccttgcggt ccgaatgggc cgaacccgct
7141 cgtctggcta agatcggccg cagcgatcgc atccatagcc tccgcgaccg gttgtagaac
7201 agcgggcagt tcggtttcag gcaggtcttg caacgtgaca ccctgtgaac ggcgggagat
7261 gcaataggtc aggctctcgc taaactcccc aatgtcaagc acttccggaa tcgggagcgc
7321 ggccgatgca aagtgccgat aaacataacg atctttgtag aaaccatcgg cgcagctatt
7381 tacccgcagg acatatccac gccctcctac atcgaagctg aaagcacgag attcttcgcc
7441 ctccgagagc tgcatcaggt cggacacgct gtcgaacttt tcgatcagaa acttctcgac
7501 agacgtcgcg gtgagttcag gctttttcat aagcttctgc aaaagagaac cagacaacag
7561 ggtaagtgcc tagcagtaaa caaacagaac tcatcacaag caaacagcaa catcatattc
7621 ataccaacag gtcatgtgtg ttcatcacat cattagtact aagcatgcca tcatccaagt
7681 atatcaaagt aagggcaaag agcatgcatg atcatcaggt gcacaaaaga atcatcaaat
7741 tgtagcagta catatcttca tctatcatgc atatctatcc ataacaggac gatgcatgtt
7801 gaccaggtaa aagctacagg atcctatagg aacagcagcg tatatatctt caccaatctt
7861 agcatcttaa tcatgtggca catgcagttt caatttaagc acatgagcta gttgattatg
7921 aggtaccaga gaatcatcaa actgatgtag cagcatatat cctcatctat catgcaatct
7981 aatctaatct aactaaacag gaaaggtgtg ctattcagtt aaaagctacc gcatcatata
8041 caaacggcag cataagaaaa agcataatca tcttaatcat gcaacaaacg cagattcata
8101 ataagcccaa gagctagctt gtgatgatct tattctactc tgatctacag caatcagata
8161 acgacctaac cttgcacatg gcaacaaaac aatcgatcgg acgaatcagt tgtttgttcc
8221 tagctagcac catcgaacca gataatagat gcacgtacag atcccgaaaa cgaacccaaa
8281 aacagggcag acctagctga acctaggcag cgacccagca gatcgtgaga acgatctcat
8341 ctacgaacag cctagaagca accccacgat tcccggacaa acgacctaaa atccccacaa
8401 atcacatgag catgacagga taaacagcgg aaccgatcag atctacacga aaaccccacc
8461 tcccagccac ccacgatcag gaaacacgcg gatctagcat gatttcgtca acgcctcagc
8521 ctagttccta gccacagacc aagcagaacc accaaaccac gccgagcgag gagatggggc
8581 aagaggacgg gggagacgat cgccgtacct tgaagcgggg gaaggatcgc cgagggtcgc
8641 gaggagagca atttggattt ggaacccggg ggttgtgcgc tccgaacgat gagacgatgt
8701 gagattgtgg gaagaggcgc ggagggccct gtatttatgg gctgcgacgg ggggaggaga
8761 ggtggggagg gttggggaag gaatccccca cccgtgccgt gacggttccg ggccgtgtga
8821 gaggagcccg ctcgtctccg ccacgcaatt tccgcgatcg gagcggagct ttcgagaggc
8881 ggctggatgg ttggtggccg ttagatttgt agacgccgtt aacgcctcgc ctccaccggg
8941 aagagttttg agcagccgct tatgacaatg gcttaacgac gttagacgga gcgttagtgg
9001 caggccatga tagaatagac gtattgcaat gggattataa ttaaataaat aagaatataa
9061 taagatatgg caagtcggca ctcatgacat ggtcttcgaa atgatagtgc tcactttctt
9121 agccgagaaa gttgacgcga ctgatttaga agttaagata ttatttctct cttcttttct
9181 ttcctcgtca tataaggatg aaataaactt tagagattgc cggtagtgat tttggatttc
9241 ggcgatcagg cttggtttgc cggtttcgga cggtgtgcct taggccaccc gcagtgtatc
9301 ttgtaatgtt caaccgataa gcaagggtgg ggctcaagca agtagtaaac aactatgtca
9361 aatgtcacca tggttatggt cttgtttagt tggcttcttg gccgattcat taatgcagct
9421 ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
9481 agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
9541 gaattgtgag cggataacaa tttcacacag gaaacagcta tgacatgatt acgaattcga
9601 gctcggtacc cggggatctg caggcgcgtg cagcgtgacc cggtcgtgcc cctctctaga
9661 gataatgagc attgcatgtc taagttataa aaaattacca catatttttt ttgtcacact
9721 tgtttgaagt gcagtttatc tatctttata catatattta aactttactc tacgaataat
9781 ataatctata gtactacaat aatatcagtg ttttagagaa tcatataaat gaacagttag
9841 acatggtcta aaggacaatt gagtattttg acaacaggac tctacagttt tatcttttta
9901 gtgtgcatgt gttctccttt ttttttgcaa atagcttcac ctatataata cttcatccat
9961 tttattagta catccattta gggtttaggg ttaatggttt ttatagacta atttttttag
10021 tacatctatt ttattctatt ttagcctcta aattaagaaa actaaaactc tattttagtt
10081 tttttattta ataatttaga tataaaatag aataaaataa agtgactaaa aattaaacaa
10141 atacccttta agaaattaaa aaaactaagg aaacattttt cttgtttcga gtagataatg
10201 ccagcctgtt aaacgccgtc gacgagtcta acggacacca accagcgaac cagcagcgtc
10261 gcgtcgggcc aagcgaagca gacggcacgg catctctgtc gctgcctctg gacccctctc
10321 gagagttccg ctccaccgtt ggacttgctc cgctgtcggc atccagaaat tgcgtggcgg
10381 agcggcagac gtgagccggc acggcaggcg gcctcctcct cctctcacgg caccggcagc
10441 tacgggggat tcctttccca ccgctccttc gctttccctt cctcgcccgc cgtaataaat
10501 agacaccccc tccacaccct ctttccccaa cctcgtgttg ttcggagcgc acacacacac
10561 aaccagatct cccccaaatc cacccgtcgg cacctccgct tcaaggtacg ccgctcgtcc
10621 tccccccccc cccctctcta ccttctctag atcggcgttc cggtccatgg ttagggcccg
10681 gtagttctac ttctgttcat gtttgtgtta gatccgtgtt tgtgttagat ccgtgctgct
10741 agcgttcgta cacggatgcg acctgtacgt cagacacgtt ctgattgcta acttgccagt
10801 gtttctcttt ggggaatcct gggatggctc tagccgttcc gcagacggga tcgatttcat
10861 gatttttttt gtttcgttgc atagggtttg gtttgccctt ttcctttatt tcaatatatg
10921 ccgtgcactt gtttgtcggg tcatcttttc atgctttttt ttgtcttggt tgtgatgatg
10981 tggtctggtt gggcggtcgt tctagatcgg agtagaattc tgtttcaaac tacctggtgg
11041 atttattaat tttggatctg tatgtgtgtg ccatacatat tcatagttac gaattgaaga
11101 tgatggatgg aaatatcgat ctaggatagg tatacatgtt gatgcgggtt ttactgatgc
11161 atatacagag atgctttttg ttcgcttggt tgtgatgatg tggtgtggtt gggcggtcgt
11221 tcattcgttc tagatcggag tagaatactg tttcaaacta cctggtgtat ttattaattt
11281 tggaactgta tgtgtgtgtc atacatcttc atagttacga gtttaagatg gatggaaata
11341 tcgatctagg ataggtatac atgttgatgt gggttttact gatgcatata catgatggca
11401 tatgcagcat ctattcatat gctctaacct tgagtaccta tctattataa taaacaagta
11461 tgttttataa ttattttgat cttgatatac ttggatgatg gcatatgcag cagctatatg
11521 tggatttttt tagccctgcc ttcatacgct atttatttgc ttggtactgt ttcttttgtc
11581 gatgctcacc ctgttgtttg gtgttacttc tgcagactag agttatcaac aagtttgtac
11641 aaaaaagctg ccaccatggc gatgccgtat gcctctcttt ccccggcagg cgaccgccgc
11701 tcctccccgg ccgccaccgc caccgcctcc ctcctcccct tctgccgctc ctcccccttc
11761 tccgccggcg gcaatggcgg catgggggag gaggcgcgga tggacgggag gtggatggcg
11821 aggccggtgc ccttcacggc ggcgcagtac gaggagctgg agcaccaggc gctcatatac
11881 aagtacctgg tggccggcgt gtccgtcccg ccggatctcg tgctccccat ccgccgcggc
11941 atcgagtccc tcgccgcccg cttctaccac aaccccctcg ccatcgggta cggatcgtac
12001 ctgggcaaga aggtggatcc ggagccgggc cggtgccggc gcacggacgg caagaagtgg
12061 cggtgcgcca aggaggccgc ctccgactcc aagtattgcg agcgccacat gcaccgcggc
12121 cgcaaccgtt caagaaagcc tgtggaaacg cagctcgtct cgcactccca gccgccggcc
12181 gcctccgtcg tgccgcccct cgccaccggc ttccacaacc actccctcta ccccgccatc
12241 ggcggcacca acggtggtgg aggcgggggg aacaacggca tgtccatgcc cggcacgttc
12301 tcctccgcgc tggggccgcc tcagcagcac atgggcaaca atgccgcctc tccctacgcg
12361 gctctcggcg gcgccggaac atgcaaagat ttcaggtata ccgcatatgg aataagatct
12421 ttggcagacg agcagagtca gctcatgaca gaagccatga acacctccgt ggagaaccca
12481 tggcgcctgc cgccatcttc tcaaacgact acattcccgc tctcaagcta ctctcctcag
12541 cttggagcaa cgagtgacct gggtcagaac aacagcagca acaacaacag cggcgtcaag
12601 gccgagggac agcagcagca gcagccgctc tccttcccgg ggtgcggcga cttcggcagc
12661 ggcgactccg cgaagcagga gaaccagacg ctgcggccgt tcttcgacga gtggccgaag
12721 acgagggact cgtggtcgga cctgaccgac gacaactcga acgtcgcctc cttctcggcc
12781 acccagctgt cgatctcgat acccatgacg tcctccgact tctccgccgc cagctcccag
12841 tcgcccaacg gcatgctgtt cgccggcgaa atgtacgcgg ccgctgccat gcagcagcaa
12901 cacctgatgc agatgaacca gagcatgatg gggggctacg cttcctctac cactgccacc
12961 actgatctca ttcagcagta cctggatgag aacaagcagc tgatcctggc catcctcgac
13021 aaccagaaca acggcaaggt ggaggagtgc gcacggaacc aagctaagct ccagcagaac
13081 ctcatgtacc tcgccgccat cgccgacagc cagcctccgc agacggcatc gctgtctcag
13141 tacccgtcca acctgatgat gcagtccggg ccgcggtaca tgcagcagca gtcggcgcag
13201 atgatgtcgc cgcagtcgct gatggcggcg cggtcgtcga tgatgtacgc gcagcaggcc
13261 atgtcgccgc tccagcagca gcagcagcag cagcagcacc aggcggccgc gcacggccag
13321 ctggggatgt cctccggcgc gaccaccggg ttcaacctcc tgcacggtga ggccagcatg
13381 ggcggcggcg gcggcgccac tggcaacagc atgatgaacg ccagcgtctt ctcggactat
13441 ggccgcggcg gcagcggcgc caaggagggg tcgacctcgc tgtcggccga cgctcgcggc
13501 gccaactctg gcgcgcacag cggcgacggg gagtacctca agggcaccga ggaggaagga
13561 agctagcgtt cagctttctt gtacaaagtg gttgataaca gcgggttaat taacatcttt
13621 tacccatacg atgttcctga ctatgcgggc tatccctatg acgtcccgga ctatgcagga
13681 tcctatccat atgacgttcc agattacgct gctcagtgca gcgcttagag ctcgaatttc
13741 cccgatcgtt caaacatttg gcaataaagt ttcttaagat tgaatcctgt tgccggtctt
13801 gcgatgatta tcatataatt tctgttgaat tacgttaagc atgtaataat taacatgtaa
13861 tgcatgacgt tatttatgag atgggttttt atgattagag tcccgcaatt atacatttaa
13921 tacgcgatag aaaacaaaat atagcgcgca aactaggata aattatcgcg cgcggtgtca
13981 tctatgttac tagatcgggg attagcgcgc ctgcagtgca gcgtgacccg gtcgtgcccc
14041 tctctagaga taatgagcat tgcatgtcta agttataaaa aattaccaca tatttttttt
14101 gtcacacttg tttgaagtgc agtttatcta tctttataca tatatttaaa ctttactcta
14161 cgaataatat aatctatagt actacaataa tatcagtgtt ttagagaatc atataaatga
14221 acagttagac atggtctaaa ggacaattga gtattttgac aacaggactc tacagtttta
14281 tctttttagt gtgcatgtgt tctccttttt ttttgcaaat agcttcacct atataatact
14341 tcatccattt tattagtaca tccatttagg gtttagggtt aatggttttt atagactaat
14401 ttttttagta catctatttt attctatttt agcctctaaa ttaagaaaac taaaactcta
14461 ttttagtttt tttatttaat aatttagata taaaatagaa taaaataaag tgactaaaaa
14521 ttaaacaaat accctttaag aaattaaaaa aactaaggaa acatttttct tgtttcgagt
14581 agataatgcc agcctgttaa acgccgtcga cgagtctaac ggacaccaac cagcgaacca
14641 gcagcgtcgc gtcgggccaa gcgaagcaga cggcacggca tctctgtcgc tgcctctgga
14701 cccctctcga gagttccgct ccaccgttgg acttgctccg ctgtcggcat ccagaaattg
14761 cgtggcggag cggcagacgt gagccggcac ggcaggcggc ctcctcctcc tctcacggca
14821 ccggcagcta cgggggattc ctttcccacc gctccttcgc tttcccttcc tcgcccgccg
14881 taataaatag acaccccctc cacaccctct ttccccaacc tcgtgttgtt cggagcgcac
14941 acacacacaa ccagatctcc cccaaatcca cccgtcggca cctccgcttc aaggtacgcc
15001 gctcgtcctc cccccccccc ctctctacct tctctagatc ggcgttccgg tccatggtta
15061 gggcccggta gttctacttc tgttcatgtt tgtgttagat ccgtgtttgt gttagatccg
15121 tgctgctagc gttcgtacac ggatgcgacc tgtacgtcag acacgttctg attgctaact
15181 tgccagtgtt tctctttggg gaatcctggg atggctctag ccgttccgca gacgggatcg
15241 atttcatgat tttttttgtt tcgttgcata gggtttggtt tgcccttttc ctttatttca
15301 atatatgccg tgcacttgtt tgtcgggtca tcttttcatg cttttttttg tcttggttgt
15361 gatgatgtgg tctggttggg cggtcgttct agatcggagt agaattaatt ctgtttcaaa
15421 ctacctggtg gatttattaa ttttggatct gtatgtgtgt gccatacata ttcatagtta
15481 cgaattgaag atgatggatg gaaatatcga tctaggatag gtatacatgt tgatgcgggt
15541 tttactgatg catatacaga gatgcttttt gttcgcttgg ttgtgatgat gtggtgtggt
15601 tgggcggtcg ttcattcgtt ctagatcgga gtagaatact gtttcaaact acctggtgta
15661 tttattaatt ttggaactgt atgtgtgtgt catacatctt catagttacg agtttaagat
15721 ggatggaaat atcgatctag gataggtata catgttgatg tgggttttac tgatgcatat
15781 acatgatggc atatgcagca tctattcata tgctctaacc ttgagtacct atctattata
15841 ataaacaagt atgttttata attattttga tcttgatata cttggatgat ggcatatgca
15901 gcagctatat gtggattttt ttagccctgc cttcatacgc tatttatttg cttggtactg
15961 tttcttttgt cgatgctcac cctgttgttt ggtgttactt cctgcagggc gatctattcg
16021 aatggacaag aagtactcga tcggcctcga catcgggacg aactcagttg gctgggccgt
16081 gatcaccgac gagtacaagg tgccctctaa gaagttcaag gtcctgggga acaccgaccg
16141 ccattccatc aagaagaacc tcatcggcgc tctcctgttc gacagcgggg agaccgctga
16201 ggctacgagg ctcaagagaa ccgctaggcg ccggtacacg agaaggaaga acaggatctg
16261 ctacctccaa gagattttct ccaacgagat ggccaaggtt gacgattcat tcttccaccg
16321 cctggaggag tctttcctcg tggaggagga taagaagcac gagcggcatc ccatcttcgg
16381 caacatcgtg gacgaggttg cctaccacga gaagtaccct acgatctacc atctgcggaa
16441 gaagctcgtg gactccaccg ataaggcgga cctcagactg atctacctcg ctctggccca
16501 catgatcaag ttccgcggcc atttcctgat cgagggggat ctcaacccag acaacagcga
16561 tgttgacaag ctgttcatcc aactcgtgca gacctacaac caactcttcg aggagaaccc
16621 gatcaacgcc tctggcgtgg acgcgaaggc tatcctgtcc gcgaggctct cgaagtccag
16681 gaggctggag aacctgatcg ctcagctccc aggcgagaag aagaacggcc tgttcgggaa
16741 cctcatcgct ctcagcctgg ggctcacccc gaacttcaag tcgaacttcg atctcgctga
16801 ggacgccaag ctgcaactct ccaaggacac ctacgacgat gacctcgata acctcctggc
16861 ccagatcggc gatcaatacg cggacctgtt cctcgctgcc aagaacctgt cggacgccat
16921 cctcctgtca gatatcctcc gcgtgaacac cgagatcacg aaggctccac tctctgcctc
16981 catgatcaag cgctacgacg agcaccatca ggatctgacc ctcctgaagg cgctggtccg
17041 ccaacagctc ccggagaagt acaaggagat tttcttcgat cagtcgaaga acggctacgc
17101 tgggtacatc gacggcgggg cctcacaaga ggagttctac aagttcatca agccaatcct
17161 ggagaagatg gacggcacgg aggagctcct ggtgaagctc aacagggagg acctcctgcg
17221 gaagcagaga accttcgata acggcagcat cccccaccaa atccatctcg gggagctgca
17281 cgccatcctg agaaggcaag aggacttcta ccctttcctc aaggataacc gggagaagat
17341 cgagaagatc ctgaccttca gaatcccata ctacgtcggc cctctcgcgc gggggaactc
17401 aagattcgct tggatgaccc gcaagtctga ggagaccatc acgccgtgga acttcgagga
17461 ggtggtggac aagggcgcta gcgctcagtc gttcatcgag aggatgacca acttcgacaa
17521 gaacctgccc aacgagaagg tgctccctaa gcactcgctc ctgtacgagt acttcaccgt
17581 ctacaacgag ctcacgaagg tgaagtacgt caccgagggc atgcgcaagc cagcgttcct
17641 gtccggggag cagaagaagg ctatcgtgga cctcctgttc aagaccaacc ggaaggtcac
17701 ggttaagcaa ctcaaggagg actacttcaa gaagatcgag tgcttcgatt cggtcgagat
17761 cagcggcgtt gaggaccgct tcaacgccag cctcgggacc taccacgatc tcctgaagat
17821 catcaaggat aaggacttcc tggacaacga ggagaacgag gatatcctgg aggacatcgt
17881 gctgaccctc acgctgttcg aggacaggga gatgatcgag gagcgcctga agacgtacgc
17941 ccatctcttc gatgacaagg tcatgaagca actcaagcgc cggagataca ccggctgggg
18001 gaggctgtcc cgcaagctca tcaacggcat ccgggacaag cagtccggga agaccatcct
18061 cgacttcctg aagagcgatg gcttcgccaa caggaacttc atgcaactga tccacgatga
18121 cagcctcacc ttcaaggagg atatccaaaa ggctcaagtg agcggccagg gggactcgct
18181 gcacgagcat atcgcgaacc tcgctggctc ccccgcgatc aagaagggca tcctccagac
18241 cgtgaaggtt gtggacgagc tcgtgaaggt catgggccgg cacaagcctg agaacatcgt
18301 catcgagatg gccagagaga accaaaccac gcagaagggg caaaagaact ctagggagcg
18361 catgaagcgc atcgaggagg gcatcaagga gctggggtcc caaatcctca aggagcaccc
18421 agtggagaac acccaactgc agaacgagaa gctctacctg tactacctcc agaacggcag
18481 ggatatgtac gtggaccaag agctggatat caaccgcctc agcgattacg acgtcgatca
18541 tatcgttccc cagtctttcc tgaaggatga ctccatcgac aacaaggtcc tcaccaggtc
18601 ggacaagaac cgcggcaagt cagataacgt tccatctgag gaggtcgtta agaagatgaa
18661 gaactactgg aggcagctcc tgaacgccaa gctgatcacg caaaggaagt tcgacaacct
18721 caccaaggct gagagaggcg ggctctcaga gctggacaag gccggcttca tcaagcggca
18781 gctggtcgag accagacaaa tcacgaagca cgttgcgcaa atcctcgact ctcggatgaa
18841 cacgaagtac gatgagaacg acaagctgat cagggaggtt aaggtgatca ccctgaagtc
18901 taagctcgtc tccgacttca ggaaggattt ccagttctac aaggttcgcg agatcaacaa
18961 ctaccaccat gcccatgacg cttacctcaa cgctgtggtc ggcaccgctc tgatcaagaa
19021 gtacccaaag ctggagtccg agttcgtgta cggggactac aaggtttacg atgtgcgcaa
19081 gatgatcgcc aagtcggagc aagagatcgg caaggctacc gccaagtact tcttctactc
19141 aaacatcatg aacttcttca agaccgagat cacgctggcc aacggcgaga tccggaagag
19201 accgctcatc gagaccaacg gcgagacggg ggagatcgtg tgggacaagg gcagggattt
19261 cgcgaccgtc cgcaaggttc tctccatgcc ccaggtgaac atcgtcaaga agaccgaggt
19321 ccaaacgggc gggttctcaa aggagtctat cctgcctaag cggaacagcg acaagctcat
19381 cgccagaaag aaggactggg acccaaagaa gtacggcggg ttcgacagcc ctaccgtggc
19441 ctactcggtc ctggttgtgg cgaaggttga gaagggcaag tccaagaagc tcaagagcgt
19501 gaaggagctc ctggggatca ccatcatgga gaggtccagc ttcgagaaga acccaatcga
19561 cttcctggag gccaagggct acaaggaggt gaagaaggac ctgatcatca agctcccgaa
19621 gtactctctc ttcgagctgg agaacggcag gaagagaatg ctggcttccg ctggcgagct
19681 ccagaagggg aacgagctcg cgctgccaag caagtacgtg aacttcctct acctggcttc
19741 ccactacgag aagctcaagg gcagcccgga ggacaacgag caaaagcagc tgttcgtcga
19801 gcagcacaag cattacctcg acgagatcat cgagcaaatc tccgagttca gcaagcgcgt
19861 gatcctcgcc gacgcgaacc tggataaggt cctctccgcc tacaacaagc accgggacaa
19921 gcccatcaga gagcaagcgg agaacatcat ccatctcttc accctgacga acctcggcgc
19981 tcctgctgct ttcaagtact tcgacaccac gatcgatcgg aagagataca cctccacgaa
20041 ggaggtcctg gacgcgaccc tcatccacca gtcgatcacc ggcctgtacg agacgaggat
20101 cgacctctca caactcggcg gggataagag acccgcagca accaagaagg cagggcaagc
20161 aaagaagaag aagtgactcg agatatgaag atgaagatga aatatttggt gtgtcaaata
20221 aaaagcttgt gtgcttaagt ttgtgttttt ttcttggctt gttgtgttat gaatttgtgg
20281 ctttttctaa tattaaatga atgtaagatc acattataat gaataaacaa atgtttctat
20341 aatccattgt gaatgttttg ttggatctct tctgcagcat ataactactg tatgtgctat
20401 ggtatggact atggaatatg attaaagata aggagctccg gtgacggacg accaagcccg
20461 ttattctgac agttctggtg ctcaacacat ttatatttat caaggagcac attgttactc
20521 actgctagga gggaatcgaa ctaggaatat tgatcagagg aactacgaga gagctgaaga
20581 taactgccct ctagctctca ctgatctggg tcgcatagtg agatgcagcc cacgtgagtt
20641 cagcaacggt ctagcgctgg gcttttaggc ccgcatgatc gggcttttgt cgggtggtcg
20701 acgtgttcac gattggggag agcaacgcag cagttcctct tagtttagtc ccacctcgcc
20761 tgtccagcag agttctgacc ggtttataaa ctcgcttgct gcatcagact tagaggcagg
20821 tgccgctaca gggcgcgtcc cattcgccat tcaggctgcg caactgttgg gaagggcgat
20881 cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
20941 taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgagc
21001 gcgcgtaata cgactcacta tagggcgaat tgggtaccgg gccccccctc gaggtcctcc
21061 agcttttgtt ccctttagtg agggttaatt gcgcgcttgg cgtaatcatg gtcatagctg
21121 tttcctgtgt gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata
21181 aagtgtaaag cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca
21241 ctgcccgctt tccacctgcg gtcgttttag agctagaaat agcaagttaa aataaggcta
21301 gtccgttatc aacttgaaaa agtggcaccg agtcggtgct ttttttcccg gtggcactgg
21361 ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt aatcgccttg
21421 cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc gatcgccctt
21481 cccaacagtt gcgcagcctg aatggcgaat gctagagcag cttgagcttg gatcagattg
21541 tcgtttcccg ccttcagttt aaactatcag tgtttgacag gatatattgg cgggtaaacc
21601 taagagaaaa gagcgtttat tagaataatc ggatatttaa aagggcgtga aaaggtttat
21661 ccgttcg
//
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JD633质粒载体应用举例
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