pCAG-VSVL

LOCUS       pCAG-VSVL               11125 bp ds-DNA     circular SYN 12-MAY-2021
DEFINITION  Expression vector for Vesicular Stomatitis Virus Indiana strain 
            polymerase gene.
ACCESSION   .
VERSION     .
KEYWORDS    pCAG-VSVL
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11125)
  TITLE     Genetic Neuroengineering Group Plasmids
  JOURNAL   Unpublished
REFERENCE   2  (bases 1 to 11125)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported May 12, 2021 from SnapGene Server 1.1.58
            http://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..11125
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     enhancer        4..383
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        386..661
                     /label=chicken beta-actin promoter
     intron          662..1670
                     /label=chimeric intron
                     /note="chimera between introns from chicken beta-actin and 
                     rabbit beta-globin"
     primer_bind     1594..1616
                     /label=pCAGGS-5
                     /note="Chimeric intron in CAG promoter, forward primer"
     primer_bind     1678..1697
                     /label=pCAG-F
                     /note="Rabbit beta-globin intron, for pCAG plasmids, 
                     forward primer"
     regulatory      1728..1737
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     primer_bind     complement(8149..8168)
                     /label=Bglob-pA-R
                     /note="Rabbit beta-globin polyA region, reverse primer"
     polyA_signal    8214..8269
                     /label=-beta-globin poly(A) signal
                     /note="rabbit beta-globin polyadenylation signal (Gil and 
                     Proudfoot, 1987)"
     primer_bind     complement(8268..8287)
                     /label=rbglobpA-R
                     /note="Rabbit beta-globin polyA, reverse primer. Also 
                     called rb-glob-pA-term-R"
     primer_bind     complement(8628..8644)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(8628..8644)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(8641..8663)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    8652..8668
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(8676..8706)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8721..8742
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        8801..8996
                     /label=SV40 promoter
                     /note="SV40 early promoter"
     rep_origin      8847..8982
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     8909..8928
                     /label=SV40pro-F
                     /note="SV40 promoter/origin, forward primer"
     polyA_signal    9002..9136
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     complement(9039..9058)
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     9093..9112
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     complement(9204..9221)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9375..9963)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9455..9474)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(10134..10994)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10757..10776
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10995..11099)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 gtcgacattg attattgact agttattaat agtaatcaat tacggggtca ttagttcata
       61 gcccatatat ggagttccgc gttacataac ttacggtaaa tggcccgcct ggctgaccgc
      121 ccaacgaccc ccgcccattg acgtcaataa tgacgtatgt tcccatagta acgccaatag
      181 ggactttcca ttgacgtcaa tgggtggact atttacggta aactgcccac ttggcagtac
      241 atcaagtgta tcatatgcca agtacgcccc ctattgacgt caatgacggt aaatggcccg
      301 cctggcatta tgcccagtac atgaccttat gggactttcc tacttggcag tacatctacg
      361 tattagtcat cgctattacc atgggtcgag gtgagcccca cgttctgctt cactctcccc
      421 atctcccccc cctccccacc cccaattttg tatttattta ttttttaatt attttgtgca
      481 gcgatggggg cggggggggg gggggcgcgc gccaggcggg gcggggcggg gcgaggggcg
      541 gggcggggcg aggcggagag gtgcggcggc agccaatcag agcggcgcgc tccgaaagtt
      601 tccttttatg gcgaggcggc ggcggcggcg gccctataaa aagcgaagcg cgcggcgggc
      661 gggagtcgct gcgttgcctt cgccccgtgc cccgctccgc gccgcctcgc gccgcccgcc
      721 ccggctctga ctgaccgcgt tactcccaca ggtgagcggg cgggacggcc cttctcctcc
      781 gggctgtaat tagcgcttgg tttaatgacg gctcgtttct tttctgtggc tgcgtgaaag
      841 ccttaaaggg ctccgggagg gccctttgtg cgggggggag cggctcgggg ggtgcgtgcg
      901 tgtgtgtgtg cgtggggagc gccgcgtgcg gcccgcgctg cccggcggct gtgagcgctg
      961 cgggcgcggc gcggggcttt gtgcgctccg cgtgtgcgcg aggggagcgc ggccgggggc
     1021 ggtgccccgc ggtgcggggg ggctgcgagg ggaacaaagg ctgcgtgcgg ggtgtgtgcg
     1081 tgggggggtg agcagggggt gtgggcgcgg cggtcgggct gtaacccccc cctgcacccc
     1141 cctccccgag ttgctgagca cggcccggct tcgggtgcgg ggctccgtgc ggggcgtggc
     1201 gcggggctcg ccgtgccggg cggggggtgg cggcaggtgg gggtgccggg cggggcgggg
     1261 ccgcctcggg ccggggaggg ctcgggggag gggcgcggcg gccccggagc gccggcggct
     1321 gtcgaggcgc ggcgagccgc agccattgcc ttttatggta atcgtgcgag agggcgcagg
     1381 gacttccttt gtcccaaatc tggcggagcc gaaatctggg aggcgccgcc gcaccccctc
     1441 tagcgggcgc gggcgaagcg gtgcggcgcc ggcaggaagg aaatgggcgg ggagggcctt
     1501 cgtgcgtcgc cgcgccgccg tccccttctc catctccagc ctcggggctg ccgcaggggg
     1561 acggctgcct tcggggggga cggggcaggg cggggttcgg cttctggcgt gtgaccggcg
     1621 gctctagagc ctctgctaac catgttcatg ccttcttctt tttcctacag ctcctgggca
     1681 acgtgctggt tattgtgctg tctcatcatt ttggcaaaga attcgccgcc accatggaag
     1741 tccacgattt tgagaccgac gagttcaatg atttcaatga agatgactat gccacaagag
     1801 aattcctgaa tcccgatgag cgcatgacgt acttgaatca tgctgattac aatttgaatt
     1861 ctcctctaat tagtgatgat attgacaatt tgatcaggaa attcaattct cttccgattc
     1921 cctcgatgtg ggatagtaag aactgggatg gagttcttga gatgttaaca tcatgtcaag
     1981 ccaatcccat ctcaacatct cagatgcata aatggatggg aagttggtta atgtctgata
     2041 atcatgatgc cagtcaaggg tatagttttt tacatgaagt ggacaaagag gcagaaataa
     2101 catttgacgt ggtggagacc ttcatccgcg gctggggcaa caaaccaatt gaatacatca
     2161 aaaaggaaag atggactgac tcattcaaaa ttctcgctta tttgtgtcaa aagtttttgg
     2221 acttacacaa gttgacatta atcttaaatg ctgtctctga ggtggaattg ctcaacttgg
     2281 cgaggacttt caaaggcaaa gtcagaagaa gttctcatgg aacgaacata tgcaggatta
     2341 gggttcccag cttgggtcct acttttattt cagaaggatg ggcttacttc aagaaacttg
     2401 atattctaat ggaccgaaac tttctgttaa tggtcaaaga tgtgattata gggaggatgc
     2461 aaacggtgct atccatggta tgtagaatag acaacctgtt ctcagagcaa gacatcttct
     2521 cccttctaaa tatctacaga attggagata aaattgtgga gaggcaggga aatttttctt
     2581 atgacttgat taaaatggtg gaaccgatat gcaacttgaa gctgatgaaa ttagcaagag
     2641 aatcaaggcc tttagtccca caattccctc attttgaaaa tcatatcaag acttctgttg
     2701 atgaaggggc aaaaattgac cgaggtataa gattcctcca tgatcagata atgagtgtga
     2761 aaacagtgga tctcacactg gtgatttatg gatcgttcag acattggggt catcctttta
     2821 tagattatta cactggacta gaaaaattac attcccaagt aaccatgaag aaagatattg
     2881 atgtgtcata tgcaaaagca cttgcaagtg atttagctcg gattgttcta tttcaacagt
     2941 tcaatgatca taaaaagtgg ttcgtgaatg gagacttgct ccctcatgat catcccttta
     3001 aaagtcatgt taaagaaaat acatggccca cagctgctca agttcaagat tttggagata
     3061 aatggcatga acttccgctg attaaatgtt ttgaaatacc cgacttacta gacccatcga
     3121 taatatactc tgacaaaagt cattcaatga ataggtcaga ggtgttgaaa catgtccgaa
     3181 tgaatccgaa cactcctatc cctagtaaaa aggtgttgca gactatgttg gacacaaagg
     3241 ctaccaattg gaaagaattt cttaaagaga ttgatgagaa gggcttagat gatgatgatc
     3301 taattattgg tcttaaagga aaggagaggg aactgaagtt ggcaggtaga tttttctccc
     3361 taatgtcttg gaaattgcga gaatactttg taattaccga atatttgata aagactcatt
     3421 tcgtccctat gtttaaaggc ctgacaatgg cggacgatct aactgcagtc attaaaaaga
     3481 tgttagattc ctcatccggc caaggattga agtcatatga ggcaatttgc atagccaatc
     3541 acattgatta cgaaaaatgg aataaccacc aaaggaagtt atcaaacggc ccagtgttcc
     3601 gagttatggg ccagttctta ggttatccat ccttaatcga gagaactcat gaattttttg
     3661 agaaaagtct tatatactac aatggaagac cagacttgat gcgtgttcac aacaacacac
     3721 tgatcaattc aacctcccaa cgagtttgtt ggcaaggaca agagggtgga ctggaaggtc
     3781 tacggcaaaa aggatggagt atcctcaatc tactggttat tcaaagagag gctaaaatca
     3841 gaaacactgc tgtcaaagtc ttggcacaag gtgataatca agttatttgc acacagtata
     3901 aaacgaagaa atcgagaaac gttgtagaat tacagggtgc tctcaatcaa atggtttcta
     3961 ataatgagaa aattatgact gcaatcaaaa tagggacagg gaagttagga cttttgataa
     4021 atgacgatga gactatgcaa tctgcagatt acttgaatta tggaaaaata ccgattttcc
     4081 gtggagtgat tagagggtta gagaccaaga gatggtcacg agtgacttgt gtcaccaatg
     4141 accaaatacc cacttgtgct aatataatga gctcagtttc cacaaatgct ctcaccgtag
     4201 ctcattttgc tgagaaccca atcaatgcca tgatacagta caattatttt gggacatttg
     4261 ctagactctt gttgatgatg catgatcctg ctcttcgtca atcattgtat gaagttcaag
     4321 ataagatacc gggcttgcac agttctactt tcaaatacgc catgttgtat ttggaccctt
     4381 ccattggagg agtgtcgggc atgtctttgt ccaggttttt gattagagcc ttcccagatc
     4441 ccgtaacaga aagtctctca ttctggagat tcatccatgt acatgctcga agtgagcatc
     4501 tgaaggagat gagtgcagta tttggaaacc ccgagatagc caagtttcga ataactcaca
     4561 tagacaagct agtagaagat ccaacctctc tgaacatcgc tatgggaatg agtccagcga
     4621 acttgttaaa gactgaggtt aaaaaatgct taatcgaatc aagacaaacc atcaggaacc
     4681 aggtgattaa ggatgcaacc atatatttgt atcatgaaga ggatcggctc agaagtttct
     4741 tatggtcaat aaatcctctg ttccctagat ttttaagtga attcaaatca ggcacttttt
     4801 tgggagtcgc agacgggctc atcagtctat ttcaaaattc tcgtactatt cggaactcct
     4861 ttaagaaaaa gtatcatagg gaattggatg atttgattgt gaggagtgag gtatcctctt
     4921 tgacacattt agggaaactt catttgagaa ggggatcatg taaaatgtgg acatgttcag
     4981 ctactcatgc tgacacatta agatacaaat cctggggccg tacagttatt gggacaactg
     5041 taccccatcc attagaaatg ttgggtccac aacatcgaaa agagactcct tgtgcaccat
     5101 gtaacacatc agggttcaat tatgtttctg tgcattgtcc agacgggatc catgacgtct
     5161 ttagttcacg gggaccattg cctgcttatc tagggtctaa aacatctgaa tctacatcta
     5221 ttttgcagcc ttgggaaagg gaaagcaaag tcccactgat taaaagagct acacgtctta
     5281 gagatgctat ctcttggttt gttgaacccg actctaaact agcaatgact atactttcta
     5341 acatccactc tttaacaggc gaagaatgga ccaaaaggca gcatgggttc aaaagaacag
     5401 ggtctgccct tcataggttt tcgacatctc ggatgagcca tggtgggttc gcatctcaga
     5461 gcactgcagc attgaccagg ttgatggcaa ctacagacac catgagggat ctgggagatc
     5521 agaatttcga ctttttattc caagcaacgt tgctctatgc tcaaattacc accactgttg
     5581 caagagacgg atggatcacc agttgtacag atcattatca tattgcctgt aagtcctgtt
     5641 tgagacccat agaagagatc accctggact caagtatgga ctacacgccc ccagatgtat
     5701 cccatgtgct gaagacatgg aggaatgggg aaggttcgtg gggacaagag ataaaacaga
     5761 tctatccttt agaagggaat tggaagaatt tagcacctgc tgagcaatcc tatcaagtcg
     5821 gcagatgtat aggttttcta tatggagact tggcgtatag aaaatctact catgccgagg
     5881 acagttctct atttcctcta tctatacaag gtcgtattag aggtcgaggt ttcttaaaag
     5941 ggttgctaga cggattaatg agagcaagtt gctgccaagt aatacaccgg agaagtctgg
     6001 ctcatttgaa gaggccggcc aacgcagtgt acggaggttt gatttacttg attgataaat
     6061 tgagtgtatc acctccattc ctttctctta ctagatcagg acctattaga gacgaattag
     6121 aaacgattcc ccacaagatc ccaacctcct atccgacaag caaccgtgat atgggggtga
     6181 ttgtcagaaa ttacttcaaa taccaatgcc gtctaattga aaagggaaaa tacagatcac
     6241 attattcaca attatggtta ttctcagatg tcttatccat agacttcatt ggaccattct
     6301 ctatttccac caccctcttg caaatcctat acaagccatt tttatctggg aaagataaga
     6361 atgagttgag agagctggca aatctttctt cattgctaag atcaggagag gggtgggaag
     6421 acatacatgt gaaattcttc accaaggaca tattattgtg tccagaggaa atcagacatg
     6481 cttgcaagtt cgggattgct aaggataata ataaagacat gagctatccc ccttggggaa
     6541 gggaatccag agggacaatt acaacaatcc ctgtttatta tacgaccacc ccttacccaa
     6601 agatgctaga gatgcctcca agaatccaaa atcccctgct gtccggaatc aggttgggcc
     6661 aattaccaac tggcgctcat tataaaattc ggagtatatt acatggaatg ggaatccatt
     6721 acagggactt cttgagttgt ggagacggct ccggagggat gactgctgca ttactacgag
     6781 aaaatgtgca tagcagagga atattcaata gtctgttaga attatcaggg tcagtcatgc
     6841 gaggcgcctc tcctgagccc cccagtgccc tagaaacttt aggaggagat aaatcgagat
     6901 gtgtaaatgg tgaaacatgt tgggaatatc catctgactt atgtgaccca aggacttggg
     6961 actatttcct ccgactcaaa gcaggcttgg ggcttcaaat tgatttaatt gtaatggata
     7021 tggaagttcg ggattcttct actagcctga aaattgagac gaatgttaga aattatgtgc
     7081 accggatttt ggatgagcaa ggagttttaa tctacaagac ttatggaaca tatatttgtg
     7141 agagcgaaaa gaatgcagta acaatccttg gtcccatgtt caagacggtc gacttagttc
     7201 aaacagaatt tagtagttct caaacgtctg aagtatatat ggtatgtaaa ggtttgaaga
     7261 aattaatcga tgaacccaat cccgattggt cttccatcaa tgaatcctgg aaaaacctgt
     7321 acgcattcca gtcatcagaa caggaatttg ccagagcaaa gaaggttagt acatacttta
     7381 ccttgacagg tattccctcc caattcattc ctgatccttt tgtaaacatt gagactatgc
     7441 tacaaatatt cggagtaccc acgggtgtgt ctcatgcggc tgccttaaaa tcatctgata
     7501 gacctgcaga tttattgacc attagccttt tttatatggc gattatatcg tattataaca
     7561 tcaatcatat cagagtagga ccgatacctc cgaacccccc atcagatgga attgcacaaa
     7621 atgtggggat cgctataact ggtataagct tttggctgag tttgatggag aaagacattc
     7681 cactatatca acagtgtttg gcagttatcc agcaatcatt tccgattagg tgggaggcta
     7741 tttcagtaaa aggaggatac aagcagaagt ggagtactag aggtgatggg ctcccaaaag
     7801 atacccgaat ttcagactcc ttggccccaa tcgggaactg gatcagatct ttggaattgg
     7861 tccgaaacca agttcgtcta aatccattca ataagatctt gttcaatcag ctatgtcgta
     7921 cagtggataa tcatttgaag tggtcaaatt tgcgaaaaaa cacaggaatg attgaatgga
     7981 tcaatgggcg aatttcaaaa gaagaccggt ctatactgat gttgaagagt gacctacatg
     8041 aggaaaactc ttggagagat taagcggccg cactcctcag gtgcaggctg cctatcagaa
     8101 ggtggtggct ggtgtggcca atgccctggc tcacaaatac cactgagatc tttttccctc
     8161 tgccaaaaat tatggggaca tcatgaagcc ccttgagcat ctgacttctg gctaataaag
     8221 gaaatttatt ttcattgcaa tagtgtgttg gaattttttg tgtctctcac tcggaaggac
     8281 atatgggagg gcaaatcatt taaaacatca gaatgagtat ttggtttaga gtttggcaac
     8341 atatgccata tgctggctgc catgaacaaa ggtggctata aagaggtcat cagtatatga
     8401 aacagccccc tgctgtccat tccttattcc atagaaaagc cttgacttga ggttagattt
     8461 tttttatatt ttgttttgtg ttattttttt ctttaacatc cctaaaattt tccttacatg
     8521 ttttactagc cagatttttc ctcctctcct gactactccc agtcatagct gtccctcttc
     8581 tcttatgaag atccctcgac ctgcagccca agcttggcgt aatcatggtc atagctgttt
     8641 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
     8701 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
     8761 cccgctttcc agtcgggaaa cctgtcgtgc cagcggatcc gcatctcaat tagtcagcaa
     8821 ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt tccgcccatt
     8881 ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc gcctcggcct
     8941 ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt tgcaaaaagc
     9001 taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac
     9061 aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc
     9121 ttatcatgtc tggatccgct gcattaatga atcggccaac gcgcggggag aggcggtttg
     9181 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
     9241 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
     9301 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
     9361 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
     9421 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
     9481 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
     9541 ctcccttcgg gaagcgtggc gctttctcaa tgctcacgct gtaggtatct cagttcggtg
     9601 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
     9661 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
     9721 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
     9781 ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat ctgcgctctg
     9841 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
     9901 gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct
     9961 caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt
    10021 taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa
    10081 aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa
    10141 tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc
    10201 tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct
    10261 gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca
    10321 gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt
    10381 aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt
    10441 gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc
    10501 ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc
    10561 tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt
    10621 atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact
    10681 ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc
    10741 ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt
    10801 ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg
    10861 atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct
    10921 gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa
    10981 tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt
    11041 ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc
    11101 acatttcccc gaaaagtgcc acctg
//