| 质粒名称: | pAAV-CAG-mScarlet-I3-P2A-EGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000714 |
| 产品用途: | AAV, experess mScarlet-I3 and EGFP in cytoplasm of mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 腺病毒，哺乳动物表达 |
| 启动子: | CAG |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | mScarlet-I3, EGFP |
| 物种来源: | Synthetic |
| 密码子优化: | 未优化 |
买家导航
pAAV-CAG-mScarlet-I3-P2A-EGFP载体质粒基本信息
pAAV-CAG-mScarlet-I3-P2A-EGFP质粒图谱载体图谱和pAAV-CAG-mScarlet-I3-P2A-EGFP载体序列质粒序列多克隆位点信息
pAAV-CAG-mScarlet-I3-P2A-EGFP质粒载体简介
pAAV-CAG-mScarlet-I3-P2A-EGFP质粒序列载体序列
LOCUS pAAV-CAG-mScarlet-I3-P2A-EGFP 6220 bp DNA circular SYN 26-APR-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6220)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6220
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
primer_bind 919..941
/label=Seq-F
primer_bind 955..995
/label=I3-F1
regulatory 977..986
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
misc_feature 983..1669
/label=mScarlet-I3
primer_bind complement(1113..1131)
/label=mCherry-R
/note="mCherry, reverse primer"
primer_bind complement(1646..1669)
/label=CAG-mScarlet-I3-R
primer_bind 1652..1690
/label=CAG-mScarlet-I3-F
primer_bind complement(1654..1692)
/label=I3-R1
CDS 1694..1750
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1763..2479
/codon_start=1
/product="the original enhanced GFP (Yang et al., 1996)"
/label=EGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 2504..3092
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2975..2986)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 3135..3256
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3784..4372)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4543..5403)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5404..5508)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5534..5989)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggatag caccgaggca gtgatcaagg agttcatgcg
1021 gttcaaggtg cacatggagg gctccatgaa cggccacgag ttcgagatcg agggcgaggg
1081 cgagggccgc ccctacgagg gcacccagac cgccaagctg aaggtgacca agggtggccc
1141 cctgcccttc tcctgggaca tcctgtcccc tcagttcatg tacggctcca gggccttcat
1201 caagcacccc gccgacatcc ccgactactg gaagcagtcc ttccccgagg gcttcaagtg
1261 ggagcgcgtg atgatcttcg aggacggcgg caccgtgtcc gtgacccagg acacctccct
1321 ggaggacggc accctgatct acaaggtgaa gctccgcggc ggcaacttcc ctcctgacgg
1381 ccccgtaatg cagaagagga caatgggctg ggaagcatcc accgagcggt tgtaccccga
1441 ggacgtcgtg ctgaagggcg acattaagat ggccctgcgc ctgaaggacg gcggccgcta
1501 cctggcggac ttcaagacca cctacaaggc caagaagccc gtgcagatgc ccggcgcctt
1561 caacatcgac cgcaagttgg acatcacctc ccacaacgag gactacaccg tggtggaaca
1621 gtacgaacgc tccgtggccc gccactccac cggcggctcc ggtggctccc gaccggttca
1681 cgtgggaagt ggggctacaa acttctcact gctgaaacag gccggggatg tggaggaaaa
1741 cccaggacca cacgtgacta gtgtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
1801 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ccggcgaggg
1861 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
1921 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
1981 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
2041 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
2101 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
2161 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
2221 ggccgacaag cagaagaacg gcatcaaggt gaacttcaag atccgccaca acatcgagga
2281 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
2341 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
2401 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
2461 ggacgagctg tacaagtaag aattcgatat caagcttatc gataatcaac ctctggatta
2521 caaaatttgt gaaagattga ctggtattct taactatgtt gctcctttta cgctatgtgg
2581 atacgctgct ttaatgcctt tgtatcatgc tattgcttcc cgtatggctt tcattttctc
2641 ctccttgtat aaatcctggt tgctgtctct ttatgaggag ttgtggcccg ttgtcaggca
2701 acgtggcgtg gtgtgcactg tgtttgctga cgcaaccccc actggttggg gcattgccac
2761 cacctgtcag ctcctttccg ggactttcgc tttccccctc cctattgcca cggcggaact
2821 catcgccgcc tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc
2881 cgtggtgttg tcggggaaat catcgtcctt tccttggctg ctcgcctgtg ttgccacctg
2941 gattctgcgc gggacgtcct tctgctacgt cccttcggcc ctcaatccag cggaccttcc
3001 ttcccgcggc ctgctgccgg ctctgcggcc tcttccgcgt cttcgccttc gccctcagac
3061 gagtcggatc tccctttggg ccgcctcccc gcatcgatac cgtcgacccg ggcggccgct
3121 tcgagcagac atgataagat acattgatga gtttggacaa accacaacta gaatgcagtg
3181 aaaaaaatgc tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag
3241 ctgcaataaa caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga
3301 gatgtgggag gttttttaaa gcaagtaaaa cctctacaaa tgtggtaaaa tcgataagga
3361 tcttcctaga gcatggctac gtagataagt agcatggcgg gttaatcatt aactacaagg
3421 aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc actgaggccg
3481 ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag
3541 cgcgcagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
3601 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
3661 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
3721 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
3781 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
3841 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
3901 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
3961 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
4021 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
4081 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
4141 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
4201 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
4261 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
4321 tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc aagaagatcc
4381 tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt aagggatttt
4441 ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa aatgaagttt
4501 taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat gcttaatcag
4561 tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct gactccccgt
4621 cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg caatgatacc
4681 gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag ccggaagggc
4741 cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta attgttgccg
4801 ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg ccattgctac
4861 aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg gttcccaacg
4921 atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc
4981 tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta tggcagcact
5041 gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg gtgagtactc
5101 aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaat
5161 acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg gaaaacgttc
5221 ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga tgtaacccac
5281 tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg ggtgagcaaa
5341 aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat gttgaatact
5401 catactcttc ctttttcaat attattgaag catttatcag ggttattgtc tcatgagcgg
5461 atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca catttccccg
5521 aaaagtgcca cctaaattgt aagcgttaat attttgttaa aattcgcgtt aaatttttgt
5581 taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta taaatcaaaa
5641 gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc actattaaag
5701 aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg cccactacgt
5761 gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact aaatcggaac
5821 cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt ggcgagaaag
5881 gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc ggtcacgctg
5941 cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc ccattcgcca
6001 ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct attacgccag
6061 ctgcgcgctc gctcgctcac tgaggccgcc cgggcaaagc ccgggcgtcg ggcgaccttt
6121 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
6181 aggggttcct tgtagttaat gattaacccg ccatgctact
//
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