| 质粒名称: | pSF11-wmiRFP680-T2A-HO1 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000102 |
| 产品用途: | Expresses miRFP680 in C. elegans neurons |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pSF11 |
| 表达系统: | 线虫表达 |
| 启动子: | tag168 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | miRFP680-T2A-HO1 |
| 物种来源: | Synthetic, Homo sapiens |
| 密码子优化: | 蠕虫表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Quantitative assessment of near-infrared fluorescent proteins
Zhang H., Papadaki S., Sun X., Wang X., Drobizhev M., Yao L., Rehbock M., Köster R.W., Wu L., Namikawa K., Piatkevich K.D.
Nat. Methods 2023 2010 20, 1605–1616 (2023)
|
买家导航
pSF11-wmiRFP680-T2A-HO1载体质粒基本信息
pSF11-wmiRFP680-T2A-HO1质粒图谱载体图谱和pSF11-wmiRFP680-T2A-HO1载体序列质粒序列多克隆位点信息
pSF11-wmiRFP680-T2A-HO1质粒载体简介
pSF11-wmiRFP680-T2A-HO1质粒序列载体序列
LOCUS pSF11-wmiRFP680-T2A-HO1 8239 bp DNA circular SYN 21-APR-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8239)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 23, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8239
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 54..2717
/label=tag-168 promoter
primer_bind 2796..2816
/label=pSF11-Fw-Seq
primer_bind 2808..2857
/label=0507-621-F
misc_feature 2834..2837
/label=optimized sequence for ribosomal binding site
misc_feature 2834..2837
/label=codon adapted insert for smGFP-myc
/note="codon adapted by
https://worm.mpi-cbg.de/codons/cgi-bin/optimize.py
Intron * 2 (3 requested, but 1 is inside myc regions and
thus removed)
CAI set to 0.60
Check all 3 boxes
"
CDS 2838..3887
/codon_start=1
/label=wmiRFP680
/translation="MAEGSVARQPDLLTCDDEPIHIPGAIQPHGLLLALAADMTIVAGS
DNLPELTGLAIGALIGRSAADVFDSETHNRLTIALAEPGAAVGAPITVGFTMRKVSLNI
YILTNPDYLNFQDAGFIGSWHRHDQLIFLELEPPQRDVAEPQAFFRRTNSAIRRLQAAE
TLESACAAAAQEVRKVSLNSSVLTNHTYLNFQITGFDRVMIYRFASDFSGEVIAEDRCA
EVESKLGLHYPASTVPAQARRLYTINPVRIIPDINYRPVPVTPDLNPVTGRPIDLSFAI
LRSVSPCHLEFMRNIGMHGTMSISILRGERLWGLIVCHHRTPYYVDLDGRQACKRVAER
LATQIGVMEE"
misc_feature 3135..3185
/label=intron-1
misc_feature 3372..3422
/label=intron-2
primer_bind complement(3865..3908)
/label=0507-621-R
CDS 3888..3908
/codon_start=1
/label=Linker
/translation="GYDIGSG"
CDS 3909..3962
/codon_start=1
/product="2A peptide from Thosea asigna virus capsid
protein"
/label=T2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="EGRGSLLTCGDVEENPGP"
CDS 3963..4982
/codon_start=1
/label=HO1
/translation="MERPQPDSMPQDLSEALKEATKEVHTQAENAEFMRNFQKGQVTRD
GFKLVMASLYHIYVALEEEIERNKVSLNIYILTNPDYLNFQESPVFAPVYFPEELHRKA
ALEQDLAFWYGPRWQEVIPYTPAMQRYVKRLHEVGRTEPELLVAHAYTRYLGDLSGGQV
LKVSLNSSVLTNHTYLNFQKIAQKALDLPSSGEGLAFFTFPNIASATKFKQLYRSRMNS
LEMTPAVRQRVIEEAKVSLNMILLTN*SDLNFQTAFLLNIQLFEELQELLTHDTKDQSP
SRAPGLRQRASNKVQDSAPVETPRGKPPLNTRSQAPLLRWVLTLSFLVATVAVGLYAM"
misc_feature 4170..4220
/label=Intron-1
primer_bind complement(4235..4257)
/label=wHO1-R
misc_feature 4458..4508
/label=Intron-2
misc_feature 4677..4727
/label=Intron-3
primer_bind complement(5024..5040)
/label=pSF11-Rv-Seq
promoter 6118..6222
/gene="bla"
/label=AmpR promoter
CDS 6223..7083
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 7178..7860
/label=ColE1 origin
protein_bind 8130..8151
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8166..8196
/label=lac promoter
/note="promoter for the E. coli lac operon"
misc_binding 8200..8222
/label=LacO
protein_bind 8204..8220
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind join(8228..8239,1..5)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 atgaccatga ttacgccaag cttgcatgcg cggccgcact gactgggccg gccggatcca
61 taggtatgcc tgtcattctc tatttttaaa cgttcctgca gcagtagcac aacagagcca
121 gcagttttcc tcttcttctc catcttcttc tgcttcttcc tctcttccca aaaaaaaaaa
181 aaaaaaaaaa gtgagggggc tcactgcccc cccccccctc ccccccccct cttctccttg
241 aagctcatcc agacgtcgct gtcctcctcc tcctcgtccc cggggcgctg tgtgtgtgtg
301 tgtgtgctcc gtgagcatgt agcagcttca gcccatttaa aggcgcacac tctctgcatc
361 tctgcgtgtc tcgacactcg ttttttctct tcttttctgc tgcacacaca cacacacaca
421 cacacacgct catgccagag cacttcttgc tccggggggc tccgcgcgga gcccgagagg
481 acctcctcct cctttttctt cttcgctttt tagttcttct tgcctcggtc tcgccccttg
541 ttttgctctc catgtagtat atacatctgg gtgtgtgtgt gtgtgtgcac ctttggagca
601 ttttttgaac ttttccagaa ctttggagca acattttcag cttgttcagt gcacttttta
661 ggctgaaaat cctattttta ggttttcgaa agtttttttg gagcattttt cgtgcaattt
721 aggtgcaaaa ttaaaaaaaa gggttttgta gtgaattaaa atttttttga aactttcgcc
781 tttttaaaaa aatgtttcta atatctttaa aaattggaat attttggaaa acaatttgtt
841 ttcaattggc aatttattaa ttctgagctt ctgagcgtaa attttttttt attttggagc
901 aattctgaga tgtcttagtg catttttcgg gcatacataa aaaaaaattt atcttaataa
961 taaaaacctt aaatttaggc ttcaccgtct ggaaattcaa ttttttcagc tgaaaaactt
1021 tcaaaaaaaa aaaaattaaa ttttcacatt tattttctct ggaattttgg agcaatttca
1081 aagctgtctt agtgcatttt tagtacacaa atattttttc atttttctta aattttaata
1141 atctaagctt aaataaaggc ttcaccgtct gaaaatgcaa cttttttcag atgaaatatt
1201 aaaaaaaaaa acctctttta ttaattttgt gctccaaact ttcccacggt acgcaaatca
1261 tgtaatatgt acttttaaac attatttttc caatcaaaaa tatccaacag cttttcctta
1321 caatttgaaa atttttatta aaatttcaaa aaaatttttt ttttttttta atttcgaatt
1381 tttttaaact tgttgtaact tttttccctg tcggcgccaa ttaacaaaat acgtagaatt
1441 ttgcgcttat tgttttgtta gttaacaaat ttttgacaac tttcttatca gctgagattt
1501 aggtgtttca gttcgcaatc tgcgtctctc cttgctctcc tctattttaa aacccccata
1561 actttgttct tgtgagagtt atcaaaaaat tgtcaactaa caaaatatag agcacaaaaa
1621 tatgcacatt ttgtatgttg acaatttttt gatacctttt ttagttttcg agatatctta
1681 gttttagttt agctgtacct ttttctggca aaaaacttcc aaatttcggc aatttttcgg
1741 caaatttccc ggcaaatttc gccaagtaca tcgggctctc agacaagtgg tctgtgagtg
1801 gaagtcgata aaagtagttt acgtgctctc cgtccccccg cttcttctcc gtctctgctt
1861 ctatttctcc attgactagt cccactactt tttacggggc ggagggctct cttgcactcc
1921 agcttcttct ctttttgtgc tcttttttgc ccgtatgtgg gtgctcattt atttttcttt
1981 ggttttaaaa taaattcggt ttattaggct actcgtaaaa atgaagaaaa aacttcaaat
2041 tcaaacgaca ttaaaaaaaa atttaataaa aattttttga attgctcaaa agccccacct
2101 atatatatat atagctccgc ctacttctcg cacagacccc gcccactttt tgactccgcc
2161 cccttattgg caaaagttct tgaaaaaaat aaaaataaaa attcaaattg aaacaatttt
2221 tttttaatga agtagttttt ttctaaattg atgaaaattt ctaaacagtt tacaagcacc
2281 gcccactttt tgactccgcc cctttgttgg aaaaattctt gtaaaaaaaa ataaaaaata
2341 aaagcttctc tttcaaacga tttttagttg gtttttgtat gaaaagtttt tttcctaaaa
2401 tagaaatgtc tgaataattt gcaagctccg cccttttctt acccagactc cgccccttta
2461 ttgactaatt tttttttgtc catttacctg aagttcaaaa aaaaaattta cagtttcata
2521 gagtaataat ttttcaaaaa tctctttttc caatgtttaa tgtgtcatta taaacccgcc
2581 cagttttaag ctccgcccat ttaggccccg cccatttatg acccaaatat ttttttctct
2641 attcacccgt ggatttccct ccaacaagtt accccaaaaa taaaaataaa aattcctgca
2701 ggcgcccacc gggatccggc gcgcctctag aggatccccg ggattggcca aaggacccaa
2761 aggtatgttt cgaatgatac taacataaca tagaacattt tcaggaggac ccttggctag
2821 cgtcgacggt accaaaaatg gccgagggat ccgtcgcccg tcaaccagac cttcttacct
2881 gcgacgacga gccaatccac atcccaggag ccatccagcc acacggactt cttcttgccc
2941 ttgccgccga catgaccatc gtcgccggat ccgacaacct tccagagctt accggacttg
3001 ccatcggagc ccttatcgga cgttccgccg ccgacgtctt cgactccgag acccacaacc
3061 gccttaccat cgcccttgcc gagccaggag ccgccgtcgg agccccaatc accgtcggat
3121 tcaccatgcg caaggtaagt ttaaacatat atatactaac taaccctgat tatttaaatt
3181 ttcaggacgc cggattcatc ggatcctggc accgtcacga ccaacttatc ttccttgagc
3241 ttgagccacc acaacgcgac gtcgccgagc cacaagcctt cttccgccgt accaactccg
3301 ccatccgtcg tcttcaagcc gccgagaccc ttgagtccgc ctgcgccgcc gccgcccaag
3361 aggtccgcaa ggtaagttta aacagttcgg tactaactaa ccatacatat ttaaattttc
3421 agatcaccgg attcgaccgc gtcatgatct accgtttcgc ctccgacttc tccggagagg
3481 tcatcgccga ggaccgttgc gccgaggtcg agtccaagct tggacttcac tacccagcct
3541 ccaccgtccc agcccaagcc cgccgccttt acaccatcaa cccagtccgc atcatcccag
3601 acatcaacta ccgcccagtc ccagtcaccc cagaccttaa cccagtcacc ggacgcccaa
3661 tcgacctttc cttcgccatc cttcgttccg tctccccatg ccaccttgag ttcatgcgca
3721 acatcggaat gcacggaacc atgtccatct ccatccttcg cggagagcgt ctttggggac
3781 ttatcgtctg ccaccaccgc accccatact acgtcgacct tgacggacgc caagcctgca
3841 agcgtgtcgc cgagcgtctt gccacccaaa tcggagtcat ggaggaggga tacgacatcg
3901 gatccggaga gggacgcgga tcccttctta cctgcggaga tgttgaggaa aacccaggac
3961 caatggagcg cccacaacca gattctatgc cacaagacct ttccgaggcc cttaaggagg
4021 ctaccaagga ggttcacacc caagctgaga acgctgagtt catgcgcaac ttccaaaagg
4081 gacaagttac ccgtgatgga ttcaagcttg tcatggcttc cctctaccac atctacgtcg
4141 ctcttgagga ggagatcgag cgcaacaagg taagtttaaa catatatata ctaactaacc
4201 ctgattattt aaattttcag gagtctccag ttttcgctcc agtttacttc ccagaagagc
4261 tccaccgtaa ggctgccctt gagcaagacc ttgccttctg gtatggacca cgctggcaag
4321 aagttatccc atacactcca gctatgcaac gctacgttaa gcgtcttcac gaggtcggac
4381 gtaccgagcc agagcttctt gtcgctcacg cttacacccg ttaccttgga gacctttccg
4441 gaggacaagt ccttaaggta agtttaaaca gttcggtact aactaaccat acatatttaa
4501 attttcagaa gatcgctcaa aaggctcttg accttccatc ttccggagag ggacttgcct
4561 tcttcacttt cccaaacatc gcttctgcca ctaagttcaa gcaactttac cgctctcgta
4621 tgaactctct tgagatgacc ccagctgtcc gccaacgtgt catcgaggag gctaaggtaa
4681 gtttaaacat gattttacta actaactaat ctgatttaaa ttttcagacc gctttccttc
4741 ttaacatcca acttttcgaa gagcttcaag agcttcttac ccacgacacc aaggaccaat
4801 ctccatcccg tgccccagga cttcgtcaac gtgcctccaa caaggtccaa gattccgctc
4861 cagttgagac tccacgtgga aagccaccac ttaacacccg ttcccaagcc ccacttcttc
4921 gttgggtcct taccctctcc ttccttgtcg ccaccgtcgc tgtcggactt tacgccatgt
4981 aagagctccg catcggccgc tgtcatcaga tcgccatctc gcgcccgtgc ctctgacttc
5041 taagtccaat tactcttcaa catccctaca tgctctttct ccctgtgctc ccacccccta
5101 tttttgttat tatcaaaaaa acttcttctt aatttctttg ttttttagct tcttttaagt
5161 cacctctaac aatgaaattg tgtagattca aaaatagaat taattcgtaa taaaaagtcg
5221 aaaaaaattg tgctccctcc ccccattaat aataattcta tcccaaaatc tacacaatgt
5281 tctgtgtaca cttcttatgt tttttttact tctgataaat tttttttgaa acatcataga
5341 aaaaaccgca cacaaaatac cttatcatat gttacgtttc agtttatgac cgcaattttt
5401 atttcttcgc acgtctgggc ctctcatgac gtcaaatcat gctcatcgtg aaaaagtttt
5461 ggagtatttt tggaattttt caatcaagtg aaagtttatg aaattaattt tcctgctttt
5521 gctttttggg ggtttcccct attgtttgtc aagagtttcg aggacggcgt ttttcttgct
5581 aaaatcacaa gtattgatga gcacgatgca agaaagatcg gaagaaggtt tgggtttgag
5641 gctcagtgga aggtgagtag aagttgataa tttgaaagtg gagtagtgtc tatggggttt
5701 ttgccttaaa tgacagaata cattcccaat ataccaaaca taactgtttc ctactagtcg
5761 gccgtacggg ccctttcgtc tcgcgcgttt cggtgatgac ggtgaaaacc tctgacacat
5821 gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca gacaagcccg
5881 tcagggcgcg tcagcgggtg ttggcgggtg tcggggctgg cttaactatg cggcatcaga
5941 gcagattgta ctgagagtgc accatatgcg gtgtgaaata ccgcacagat gcgtaaggag
6001 aaaataccgc atcaggcggc cttaattaag ggcctcgtga tacgcctatt tttataggtt
6061 aatgtcatga taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc
6121 ggaaccccta tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa
6181 taaccctgat aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc
6241 cgtgtcgccc ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa
6301 acgctggtga aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa
6361 ctggatctca acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg
6421 atgagcactt ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa
6481 gagcaactcg gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc
6541 acagaaaagc atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc
6601 atgagtgata acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta
6661 accgcttttt tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag
6721 ctgaatgaag ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca
6781 acgttgcgca aactattaac tggcgaacta cttactctag cttcccggca acaattaata
6841 gactggatgg aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc
6901 tggtttattg ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca
6961 ctggggccag atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca
7021 actatggatg aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg
7081 taactgtcag accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa
7141 tttaaaagga tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt
7201 gagttttcgt tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat
7261 cctttttttc tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg
7321 gtttgtttgc cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga
7381 gcgcagatac caaatactgt ccttctagtg tagccgtagt taggccacca cttcaagaac
7441 tctgtagcac cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt
7501 ggcgataagt cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag
7561 cggtcgggct gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc
7621 gaactgagat acctacagcg tgagcattga gaaagcgcca cgcttcccga agggagaaag
7681 gcggacaggt atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca
7741 gggggaaacg cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt
7801 cgatttttgt gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc
7861 tttttacggt tcctggcctt ttgctggcct tttgctcaca tgttctttcc tgcgttatcc
7921 cctgattctg tggataaccg tattaccgcc tttgagtgag ctgataccgc tcgccgcagc
7981 cgaacgaccg agcgcagcga gtcagtgagc gaggaagcgg aagagcgccc aatacgcaaa
8041 ccgcctctcc ccgcgcgttg gccgattcat taatgcagct ggcacgacag gtttcccgac
8101 tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt agctcactca ttaggcaccc
8161 caggctttac actttatgct tccggctcgt atgttgtgtg gaattgtgag cggataacaa
8221 tttcacacag gaaacagct
//
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