pSJX024

LOCUS       pSJX024               14318 bp ds-DNA     circular SYN 23-DEC-2024
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 14318)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Monday, Dec 23, 2024 from SnapGene 4.3.6
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..14318
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     misc_feature    458..1252
                     /standard_name="kanamycin resistance determinant"
                     /label=neo
                     /label=KanR
     misc_feature    1580..1614
                     /label=PJ23119
     misc_feature    1615..1634
                     /label=sgRNA2
     misc_feature    1635..1717
                     /label=gRNA scaffold
     misc_feature    1718..3717
                     /label=UA
     gene            1718..1824
                     /locus_tag="CCNA_02257"
                     /label=CCNA_02257
                     /db_xref="GeneID:7332622"
     gene            1910..1994
                     /locus_tag="CCNA_R0052"
                     /label=CCNA_R0052
                     /db_xref="GeneID:7332621"
     gene            2143..3612
                     /locus_tag="CCNA_02256"
                     /label=CCNA_02256
                     /db_xref="GeneID:7332620"
                     /experiment="gene is essential according to Tn-seq 
                     experiments of Christen, et al. (2011) Mol Syst Biol. 
                     30;7:528. doi: 10.1038/msb.2011.58"
     primer_bind     2829..2845
                     /label=SJX1326
     misc_feature    3718..5717
                     /label=DA
     gene            3919..5079
                     /locus_tag="CCNA_02254"
                     /label=CCNA_02254
                     /db_xref="GeneID:7332618"
     gene            complement(5188..5274)
                     /locus_tag="CCNA_03965"
                     /label=CCNA_03965
                     /db_xref="GeneID:18668899"
     gene            complement(5330..5717)
                     /locus_tag="CCNA_02253"
                     /label=CCNA_02253
                     /db_xref="GeneID:7332616"
     misc_feature    5738..5797
                     /label=rrnB T1T2
     primer_bind     5738..5754
                     /label=SJX1327
     primer_bind     complement(5771..5809)
                     /label=SJX1327
     primer_bind     5786..5815
                     /label=SJX1326
     misc_feature    5798..6157
                     /label=Pxyl
     primer_bind     complement(6140..6172)
                     /label=SJX1329
     primer_bind     6143..6180
                     /label=SJX1328
     CDS             6158..10261
                     /codon_start=1
                     /label=SpCas9M
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     primer_bind     complement(10244..10271)
                     /label=SJX1577
     primer_bind     10252..10297
                     /label=SJX1580
     misc_feature    10262..10276
                     /label=HRSAT-linker
     CDS             10277..10993
                     /codon_start=1
                     /label=sfgfp
                     /translation="MRKGEELFTGVVPILVELDGDVNGHKFSVRGEGEGDATNGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFARYPDHMKQHDFFKSAMPEGYVQERTISFKDDG
                     TYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNFNSHNVYITADKQKNGIKA
                     NFKIRHNVEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSVLSKDPNEKRDHMVLLE
                     FVTAAGITHGMDELYK"
     primer_bind     complement(10964..11003)
                     /label=SJX1582
     primer_bind     10984..11013
                     /label=SJX1581
     misc_feature    11864..12526
                     /standard_name="rep"
                     /label=rep
ORIGIN
        1 accttcggga gcgcctgaag cccgttctgg acgccctggg gccgttgaat cgggatatgc
       61 aggccaaggc cgccgcgatc atcaaggccg tgggcgaaaa gctgctgacg gaacagcggg
      121 aagtccagcg ccagaaacag gcccagcgcc agcaggaacg cgggcgcgca catttccccg
      181 aaaagtgcca cctgggatga atgtcagcta ctgggctatc tggacaaggg aaaacgcaag
      241 cgcaaagaga aagcaggtag cttgcagtgg gcttacatgg cgatagctag actgggcggt
      301 tttatggaca gcaagcgaac cggaattgcc agctggggcg ccctctggta aggttgggaa
      361 gccctgcaaa gtaaactgga tggctttctt gccgccaagg atctgatggc gcaggggatc
      421 aagatctgat caagagacag gatgaggatc gtttcgcatg attgaacaag atggattgca
      481 cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg cacaacagac
      541 aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc cggttctttt
      601 tgtcaagacc gacctgtccg gtgccctgaa tgaactgcag gacgaggcag cgcggctatc
      661 gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca ctgaagcggg
      721 aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat ctcaccttgc
      781 tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata cgcttgatcc
      841 ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac gtactcggat
      901 ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc tcgcgccagc
      961 cgaactgttc gccaggctca aggcgcgcat gcccgacggc gaggatctcg tcgtgaccca
     1021 tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg gattcatcga
     1081 ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta cccgtgatat
     1141 tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg gtatcgccgc
     1201 tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct gagcgggact
     1261 ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga tttcgattcc
     1321 accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc cggctggatg
     1381 atcctccagc gcggggatct catgctggag ttcttcgccc acccccatgg gcaaatatta
     1441 tacgcaaggc gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga
     1501 tggcttccat gtcggcagaa tgcttaatga attacaacag tttttatata agctttcagt
     1561 cggcgcgaat gctccacgct tgacagctag ctcagtccta ggtataatac tagttctctt
     1621 gatgagatcg acgggtttta gagctagaaa tagcaagtta aaataaggct agtccgttat
     1681 caacttgaaa aagtggcacc gagtcggtgc tttttttaca atcacccgat cgcccgcggc
     1741 gaaatccaga tcagcgacga tcgcatcgct atccaggtga cccgcgcggc tgacgtctac
     1801 gactacatgg ccggcggcag ctgatccgtc gcgggcttga gttcgacatt ctcgaacttc
     1861 cggcttccca aagccgattc agtttgctac cagccgcccc tcaccacgag cggtcgtggc
     1921 ggaattggta gacgcgcagc gttgaggtcg ctgtggggca acccgtggaa gttcgagtct
     1981 tctcgaccgc accaaggtga acaagagtag ggcgcgcttg gcgaaatcgg tttccgactc
     2041 gctgggggcg ctctaatcgt ttctggccct gacttcgcct cagggtcgct tgatcggacc
     2101 agcccaacga acccacgcgc tctgagagct caggaggccc ccatgacccg ggacaccgcc
     2161 gaactgaacg aagacgtgct ggacaacatc ccgctggccc atatcccgct tcccaagtcc
     2221 gagacgcccg aggagctgga agacctcgcc ctcggcgacg actacgcgct taatcccgaa
     2281 tatgtcgaga tggtgatcga cgccgccgac cgcggcgacg ccaagcgcct gcgcgagctg
     2341 gtgggggccc tggaccccgc cgacgtcgcc gacctgatgg gctttctgac ggccgactat
     2401 cgcgaggaag tgatcccgct gctcgacccc gaggcgctgg gcgagatcat ttccgagctg
     2461 gaggacaaca tccgcgagga agtgctggag gccaccccgt cggtgaccct ggcccgggcc
     2521 ctcgaggagc tggacaccga cgacgccgcc gacgttatcg acgacctgga cgacaccaag
     2581 cgcttccagg tgctggccgc catgggcgag accgaccgcg ccgccatcga gacgaccctg
     2641 tcctacgagg acgagaccgc cggccgcctg atgcagcgcg agttcctggc cgcgccggaa
     2701 ttctggaccg tcggccagac gatcgaccat gtccgcgagg ccggcgactc gctgcccgag
     2761 ctgttcttcg acgtctatgt cgtcgacccg accttcaagc ctgtcggggc gatcccgatc
     2821 agcatcctgc tgcgcagccg tcgtcagacg ccgctggccg agctgatgga ggcggtcacc
     2881 gaaatcccgg tcgagatgga ccaggaagag gtcgcctaca ttttcgacaa gtaccacctg
     2941 atcagcgccc cggtgattga accgggcggg cggctggtgg gtcagatcac tgtcgacgac
     3001 atcgtcgagg ttatccgcga cgaggccgag gaagacattc tggccctggc cggtgtgtcg
     3061 gacgccggcc gtgacgcctc ggtgatcgag atcgtcaagt cgcgcctgcc gtggctgatg
     3121 ctgaacctgg cgaccgccac cctggccgtc agcggcgtgg ccatcttcca ggccgagatc
     3181 gctcaattgg tggccctggc catcctgatg ccgatcgtct cgtccctggg cggcaacgcc
     3241 ggcacccaga ccctggccgt ggccgtgcgg gctctggcca gcaaggagct gacccccgcc
     3301 aacgcccgcc gcatcgtggc gcgggaactg ctagtgggcc tggttaacgg gctttgcctg
     3361 gcgctggtga tgggggcggc gacctacatg ttcttcgggc cgtcggatca acacgacaag
     3421 ctggcgatca tcgtcggcgc ggccctgatc accaacatct tcaccgcggc cctgggcggc
     3481 atcctgatgc ccctggcgct ggagaagatg ggccgtgacc cggcggtgtc gtcctcgatc
     3541 ttcgtcacct tcctgaccga cttcaccggc ttcttcgcgg tgctgctgat cgcctcgctg
     3601 ctgctgcact gaggttttct cgctggccac gaccctcgcc aggggaaact ggcgccgacc
     3661 ttgcgccggg acaggcgaac gcatccgcgt ctgtctcaaa ctgggtcaat ctaggcggtt
     3721 tcttgcctcg ccaccccgag gcatgagcaa gaacgcccgc tggtcccaaa gactggcggg
     3781 cgttttttgc tatcgtgcgg ctgttttctg gtggaaatcg accgagatcg gatgcacacg
     3841 ctctctcagt gttgagccag agcgtgacaa gcgctgaaac cgcccacatc ttccgctcgg
     3901 gcgttcggga aggaaaccat gtcgatgact ttcgcaccct cgatggactt cgcgctgggc
     3961 gagaccgctg acgcgatccg tgagaccacg gcccgtttcg ccgccgacaa gatcgcaccg
     4021 atcgccgcca agatcgacga gaccaacagc ttcccccgcg aactctgggt cccgatgggg
     4081 gatctgggtc tgcatggcat caccgtcgag gaagagttcg gggggctggg tctgggctat
     4141 ctcgaacacg tcgtggcgat ggaagaggtc agccgcgcct ccgcctccgt ggggctcagc
     4201 tacggcgccc actccaatct ctgcgtgaac cagatccgtc ggtgggccac gccggagcag
     4261 aaggcgcgct atctgcccaa gctgatcagc ggcgagcatg tgggctccct ggcgatgagc
     4321 gaagccggcg cgggctctga tgtcgtctcg atgaagctgc gcgccgagca ggtgggcgac
     4381 cgctacatcc tcaacggcac gaagttctgg atcaccaacg ccccgcacgc cgatacgctg
     4441 gtggtctacg ccaagaccgg cgagggcagc cgcggcatca ccgccttcat cgtcgagaag
     4501 ggcatgaagg gcttcagcgt ctccaagaag ctggacaaga tgggcatgcg cgggtcggac
     4561 accgccgagc tggtgttcga agactgcgag atccccgaag agaacgtcat gggcccggtc
     4621 ggcggtggcg tcggggtgct gatgagcggc ctcgactacg agcgggccgt gctggcggcc
     4681 ggtccgctgg gcatcatgca ggcttgcctc gatgtcgtgc tgccctacgt ccgcgaccgc
     4741 aagcagttcg gccagccgat cggctcgttc cagctgatgc agggcaagat cgccgacatg
     4801 tacgtggccc tgaactcggc ccgggcttac gtctacgcgg tggccaaggc ttgtgacgcg
     4861 ggcaagacca cgcgcttcga cgccgccggc gcgatcctga tggcgtcgga aaacgccgtg
     4921 aaggtgtcgc tggaggcgat ccaggccctc ggtggcgctg gctataccaa ggagtggccg
     4981 gtcgagcgcc tgctgcgcga cgccaagctc tatgacatcg gcgccggcac caacgagatc
     5041 cgccgcttcc tgatcggacg cgagctgatc ggcggctgat cctgggctgc gcgacatcaa
     5101 aagggccgcc ccgatcgctc ggggcggccc tccatcgtcg tagccgctct cgcagccagt
     5161 gactcgaaac ccttggcgct caacgcctta ggcggagacc tcgctcttcg ggctccaggt
     5221 cacgacttgc gaccgcggtt ggagacaatg agacactgga ccacctcctt tcagttgttg
     5281 aacaacacag gaaggatatt ggaccggttt tcgcgaaagg aaagggtcgt tatcgcgacc
     5341 aagcgggact aaacgccgcg cttcgctcgg ccaggctggg ccgcttgcga agatcggtga
     5401 tggggcgatg taccccgtcg agggcgaagc cggcggcgac gtagccggcc ttcacggcgg
     5461 gttcgaaggc gcgccagtca cggatatcga tgccttccag cttcggggtg atcagaacgt
     5521 cggtcgcctc gcgagcggcg gccagatcgc gcccggtcgt gacggtcgca gcacgcatca
     5581 gcaaggccac gatcggcggg cccttgcgcc attcgccgga gagaatccag ttccacaggg
     5641 atgggggcgt gacgatgtca gcgctggtga tgctgcggcc acgggtgacg tcgaccccga
     5701 cgatcggtcc aagctggcgc gcccagcggc gcggatccaa ataaaacgaa aggctcagtc
     5761 gaaagactgg gcctttcgtt ttatctgttg tttgtcgctg cagccagccg tggtcgggca
     5821 gcaggtagaa ggcgccctcg tcctgatcct cgcccgaaac ctccagcccc cggtcgatgg
     5881 cttcgacgac atagccggcc gcgcggcagg tgtcggtgag cgcggccagc agggcggctt
     5941 cctggtcagg ggtcaggtcg gtcatgggca agaggtccag gtcgtggttt gtcggcggct
     6001 tctagcatgg accgcccgcg cccgtgaggc cgaggatttc gcgctggtca gacaacctac
     6061 ttgccgtccc cacatgttag cgctaccaag tgccgacgaa cgcgcgccgc cgacggtgtc
     6121 ggcgcttcag acgctcgagt tttggggaga cgaccatatg gacaagaagt actcgatcgg
     6181 cctggatatc gggacgaaca gcgtcgggtg ggccgtgatc acggacgaat acaaggtgcc
     6241 ctccaagaag ttcaaggtcc tggggaatac cgaccggcac tccatcaaga agaacctcat
     6301 cggggccctg ctctttgact ccggggaaac ggccgaagcg acgcggctga agcggaccgc
     6361 gcgtcgtcgc tacacgcgcc ggaagaatcg catctgttat ctccaggaaa tcttcagcaa
     6421 cgagatggcc aaggtggatg attccttctt ccaccgcctg gaggagtcct ttctggtcga
     6481 agaggataag aagcatgagc gccacccgat ctttgggaac atcgtcgacg aagtggccta
     6541 ccacgaaaag tatcccacga tctaccacct gcgtaagaag ctcgtggact ccaccgataa
     6601 ggcggatctg cggctgatct acctcgccct ggcccacatg atcaagtttc ggggccattt
     6661 tctgatcgaa ggcgatctga atccggacaa tagcgacgtc gataagctct ttatccagct
     6721 ggtgcagacc tacaatcaac tcttcgagga gaatccgatc aatgcctccg gggtggatgc
     6781 caaggcgatc ctgagcgcgc gtctgtcgaa gtcccgccgc ctggagaacc tgatcgccca
     6841 gctcccgggc gaaaagaaga acgggctctt tgggaatctg atcgccctca gcctggggct
     6901 gaccccgaac tttaagtcca atttcgacct cgcggaagat gccaagctcc aactctcgaa
     6961 ggatacgtac gacgatgacc tcgacaacct gctggcccaa atcggcgacc agtacgcgga
     7021 cctgttcctg gcggccaaga acctgtcgga cgccatcctc ctgagcgaca tcctccgtgt
     7081 caatacggaa atcacgaagg cgccgctgtc cgccagcatg atcaagcggt atgatgagca
     7141 tcatcaagac ctcaccctgc tgaaggcgct ggtccggcaa cagctgcccg agaagtacaa
     7201 ggagatcttt ttcgatcaaa gcaagaacgg ctacgccggg tatatcgacg ggggggcctc
     7261 gcaagaagag ttctacaagt ttatcaagcc catcctggag aagatggatg ggacggagga
     7321 gctcctcgtc aagctcaacc gggaagatct cctccgcaag caacgcacct ttgataatgg
     7381 gtccatcccg catcaaatcc acctcggcga gctccatgcg atcctgcggc ggcaagagga
     7441 cttctacccg tttctcaagg acaaccgcga gaagatcgag aagatcctga cgttccgcat
     7501 cccctattat gtcggccccc tggcccgggg gaattcgcgg tttgcctgga tgacccgcaa
     7561 gtccgaagaa acgatcaccc cctggaattt tgaagaggtg gtggacaagg gggcgagcgc
     7621 gcagtccttt atcgaacgga tgaccaattt cgacaagaac ctccccaacg agaaggtgct
     7681 cccgaagcat tccctcctgt atgagtactt taccgtctac aacgagctca cgaaggtcaa
     7741 gtatgtcacc gagggcatgc gcaagcccgc cttcctctcg ggggaacaaa agaaggccat
     7801 cgtggacctg ctcttcaaga ccaaccgcaa ggtcaccgtg aagcaactca aggaggacta
     7861 tttcaagaag atcgaatgct tcgatagcgt ggagatctcc ggggtcgaag accggtttaa
     7921 cgcctcgctc ggcacgtacc atgatctcct caagatcatc aaggacaagg acttcctcga
     7981 caacgaggag aacgaagaca tcctggaaga catcgtgctg accctgaccc tcttcgagga
     8041 tcgcgagatg atcgaggagc gtctcaagac gtacgcgcat ctcttcgatg ataaggtgat
     8101 gaagcaactc aagcggcggc gttacacggg ctgggggcgc ctctcccgta agctcatcaa
     8161 cggcatccgg gacaagcaat cggggaagac catcctggac ttcctgaagt cggacggctt
     8221 tgccaatcgc aattttatgc aactcatcca cgacgattcc ctcaccttca aggaagatat
     8281 ccagaaggcc caggtgtccg ggcaagggga ctccctgcat gaacatatcg ccaacctggc
     8341 cgggagcccc gcgatcaaga aggggatcct ccagaccgtg aaggtggtcg atgagctcgt
     8401 gaaggtcatg ggccggcata agcccgaaaa tatcgtcatc gagatggcgc gcgagaacca
     8461 gaccacccaa aagggccaga agaattcgcg ggagcgcatg aagcggatcg aagaaggcat
     8521 caaggaactc ggcagccaga tcctgaagga acatccggtc gaaaacacgc agctccagaa
     8581 cgagaagctc tacctctact atctccaaaa tggccgggat atgtacgtgg accaggagct
     8641 cgacatcaat cggctcagcg actatgatgt ggatcatatc gtcccccaat cctttctcaa
     8701 ggacgacagc atcgataaca aggtcctgac ccggtcggac aagaatcgtg gcaagagcga
     8761 taacgtcccc tccgaggagg tcgtgaagaa gatgaagaac tattggcgcc aactgctcaa
     8821 cgccaagctg atcacgcaac ggaagttcga caacctcacc aaggccgagc gcgggggcct
     8881 ctcggaactg gacaaggcgg ggttcatcaa gcgccagctc gtcgaaaccc gtcaaatcac
     8941 caagcacgtg gcgcaaatcc tggatagccg catgaacacc aagtacgatg agaacgacaa
     9001 gctgatccgt gaagtgaagg tgatcaccct gaagtccaag ctcgtgagcg atttccgcaa
     9061 ggatttccaa ttttataagg tgcgggaaat caataattac catcacgcgc atgatgcgta
     9121 tctcaacgcc gtggtcggca cggccctgat caagaagtat cccaagctgg agtcggaatt
     9181 cgtctacggc gattacaagg tgtatgacgt ccgtaagatg atcgcgaaga gcgagcaaga
     9241 aatcgggaag gccacggcga agtacttctt ctacagcaat atcatgaatt tcttcaagac
     9301 ggagatcacg ctggcgaatg gcgaaatccg taagcgcccg ctgatcgaaa cgaatggcga
     9361 gaccggcgaa atcgtgtggg ataaggggcg cgatttcgcg acggtccgca aggtcctctc
     9421 gatgccgcag gtcaacatcg tcaagaagac cgaagtccag accggggggt tttccaagga
     9481 gagcatcctg ccgaagcgca acagcgacaa gctcatcgcc cgcaagaagg actgggatcc
     9541 caagaagtat ggcgggttcg atagcccgac cgtggcgtac tcggtgctgg tggtcgcgaa
     9601 ggtcgaaaag gggaagtcca agaagctcaa gagcgtcaag gaactgctgg ggatcacgat
     9661 catggaacgc tcctcctttg agaagaatcc catcgacttc ctggaggcga agggctataa
     9721 ggaggtgaag aaggatctca tcatcaagct gccgaagtat tcgctcttcg aactcgaaaa
     9781 tgggcgcaag cgcatgctgg cgtccgccgg cgagctccaa aagggcaatg agctggccct
     9841 gccctccaag tacgtcaatt ttctgtacct ggcctcccac tatgagaagc tgaagggcag
     9901 ccccgaggat aatgaacaga agcaactctt tgtcgagcaa cataagcatt acctggatga
     9961 aatcatcgag caaatcagcg aattttccaa gcgggtgatc ctcgccgatg ccaacctgga
    10021 caaggtcctc agcgcgtata acaagcaccg ggacaagccc atccgtgaac aagcggagaa
    10081 catcatccat ctgttcacgc tcaccaacct gggcgccccc gccgcgttta agtattttga
    10141 taccaccatc gatcgcaagc gttatacgtc caccaaggaa gtcctcgatg ccacgctgat
    10201 ccatcaaagc atcaccgggc tctacgagac ccggatcgat ctctcccagc tgggcggcga
    10261 tcaccggtcg gccaccatgc gtaaaggcga agagctgttc actggtgtcg tccctattct
    10321 ggtggaactg gatggtgatg tcaacggtca taagttttcc gtgcgtggcg agggtgaagg
    10381 tgacgcaact aatggtaaac tgacgctgaa gttcatctgt actactggta aactgccggt
    10441 accttggccg actctggtaa cgacgctgac ttatggtgtt cagtgctttg ctcgttatcc
    10501 ggaccatatg aagcagcatg acttcttcaa gtccgccatg ccggaaggct atgtgcagga
    10561 acgcacgatt tcctttaagg atgacggcac gtacaaaacg cgtgcggaag tgaaatttga
    10621 aggcgatacc ctggtaaacc gcattgagct gaaaggcatt gactttaaag aagacggcaa
    10681 tatcctgggc cataagctgg aatacaattt taacagccac aatgtttaca tcaccgccga
    10741 taaacaaaaa aatggcatta aagcgaattt taaaattcgc cacaacgtgg aggatggcag
    10801 cgtgcagctg gctgatcact accagcaaaa cactccaatc ggtgatggtc ctgttctgct
    10861 gccagacaat cactatctga gcacgcaaag cgttctgtct aaagatccga acgagaaacg
    10921 cgatcatatg gttctgctgg agttcgtaac cgcagcgggc atcacgcatg gtatggatga
    10981 actgtacaaa tgaatgaaaa cgggcccccc ctcgaggtcg acggtatcga taagcttgat
    11041 atcgaattcc tgcagcccgg gggatccact agttctagag cggccgccac cgcggtggag
    11101 ctccaattcg ccctatagtg agtcgtatta cgcgcgctca ctggccgtcg ttttacaacg
    11161 tcgtgactgg gaaaaccctg gcgttaccca acttaatcgc cttgcagcac atcccccttt
    11221 cgccagctgg cgtaatagcg aagaggcccg caccgatcgc ccttcccaac agttgcgcag
    11281 cctgaatggc gaatggaaat tgtaagcgtt aatattttgt taaaattcgc gttaaatttt
    11341 tgttaaatca gctcattttt taaccaatag gccgtactgc gatgagtggc agggcggggc
    11401 gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggtgctacgc ctgaataagt
    11461 gataataagc ggatgaatgg cagaaattcg aaagcaaatt cgacccggtc gtcggttcag
    11521 ggcagggtcg ttaaatagcc gcttatgtct attgctggtt taccggttta ttgactaccg
    11581 gaagcagtgt gaccgtgtgc ttctcaaatg cctgaggcca gtttgctcag gctctccccg
    11641 tggaggtaat aattgacgat atgatcattt attctgcctc ccagagcctg ataaaaacgg
    11701 tgaatccgtt agcgaggtgc cgccggcttc cattcaggtc gaggtggccc ggctccatgc
    11761 accgcgacgc aacgcgggga ggcagacaag gtatagggcg gcgaggcggc tacagccgat
    11821 agtctggaac agcgcactta cgggttgctg cgcaacccaa gtgctaccgg cgcggcagcg
    11881 tgacccgtgt cggcggctcc aacggctcgc catcgtccag aaaacacggc tcatcgggca
    11941 tcggcaggcg ctgctgcccg cgccgttccc attcctccgt ttcggtcaag gctggcaggt
    12001 ctggttccat gcccggaatg ccgggctggc tgggcggctc ctcgccgggg ccggtcggta
    12061 gttgctgctc gcccggatac agggtcggga tgcggcgcag gtcgccatgc cccaacagcg
    12121 attcgtcctg gtcgtcgtga tcaaccacca cggcggcact gaacaccgac aggcgcaact
    12181 ggtcgcgggg ctggccccac gccacgcggt cattgaccac gtaggccgac acggtgccgg
    12241 ggccgttgag cttcacgacg gagatccagc gctcggccac caagtccttg actgcgtatt
    12301 ggaccgtccg caaagaacgt ccgatgagct tggaaagtgt cttctggctg accaccacgg
    12361 cgttctggtg gcccatctgc gccacgaggt gatgcagcag cattgccgcc gtgggtttcc
    12421 tcgcaataag cccggcccac gcctcatgcg ctttgcgttc cgtttgcacc cagtgaccgg
    12481 gcttgttctt ggcttgaatg ccgatttctc tggactgcgt ggccatgctt atctccatgc
    12541 ggtagggtgc cgcacggttg cggcaccatg cgcaatcagc tgcaactttt cggcagcgcg
    12601 acaacaatta tgcgttgcgt aaaagtggca gtcaattaca gattttcttt aacctacgca
    12661 atgagctatt gcggggggtg ccgcaatgag ctgttgcgta cccccctttt ttaagttgtt
    12721 gatttttaag tctttcgcat ttcgccctat atctagttct ttggtgccca aagaagggca
    12781 cccctgcggg gttcccccac gccttcggcg cggctccccc tccggcaaaa agtggcccct
    12841 ccggggcttg ttgatcgact gcgcggcctt cggccttgcc caaggtggcg ctgccccctt
    12901 ggaacccccg cactcgccgc cgtgaggctc ggggggcagg cgggcgggct tcgccttcga
    12961 ctgcccccac tcgcataggc ttgggtcgtt ccaggcgcgt caaggccaag ccgctgcgcg
    13021 gtcgctgcgc gagccttgac ccgccttcca cttggtgtcc aaccggcaag cgaagcgcgc
    13081 aggccgcagg ccggaggctt ttccccagag aaaattaaaa aaattgatgg ggcaaggccg
    13141 caggccgcgc agttggagcc ggtgggtatg tggtcgaagg ctgggtagcc ggtgggcaat
    13201 ccctgtggtc aagctcgtgg gcaggcgcag cctgtccatc agcttgtcca gcagggttgt
    13261 ccacgggccg agcgaagcga gccagccggt ggccgctcgc ggccatcgtc cacatatcca
    13321 cgggctggca agggagcgca gcgaccgcgc agggcgaagc ccggagagca agcccgtagg
    13381 gcgccgcagc cgccgtaggc ggtcacgact ttgcgaagca aagtctagtg agtatactca
    13441 agcattgagt ggcccgccgg aggcaccgcc ttgcgctgcc cccgtcgagc cggttggaca
    13501 ccaaaaggga ggggcaggca tggcggcata cgcgatcatg cgatgcaaga agctggcgaa
    13561 aatgggcaac gtggcggcca gtctcaagca cgcctaccgc gagcgcgaga cgcccaacgc
    13621 tgacgccagc aggacgccag agaacgagca ctgggcggcc agcagcaccg atgaagcgat
    13681 gggccgactg cgcgagttgc tgccagagaa gcggcgcaag gacgctgtgt tggcggtcga
    13741 gtacgtcatg acggccagcc cggaatggtg gaagtcggcc agccaagaac agcaggcggc
    13801 gttcttcgag aaggcgcaca agtggctggc ggacaagtac ggggcggatc gcatcgtgac
    13861 ggccagcatc caccgtgacg aaaccagccc gcacatgacc gcgttcgtgg tgccgctgac
    13921 gcaggacggc aggctgtcgg ccaaggagtt catcggcaac aaagcgcaga tgacccgcga
    13981 ccagaccacg tttgcggccg ctgtggccga tctagggctg caacggggca tcgagggcag
    14041 caaggcacgt cacacgcgca ttcaggcgtt ctacgaggcc ctggagcggc caccagtggg
    14101 ccacgtcacc atcagcccgc aagcggtcga gccacgcgcc tatgcaccgc agggattggc
    14161 cgaaaagctg ggaatctcaa agcgcgttga gacgccggaa gccgtggccg accggctgac
    14221 aaaagcggtt cggcaggggt atgagcctgc cctacaggcc gccgcaggag cgcgtgagat
    14281 gcgcaagaag gccgatcaag cccaagagac ggcccgag
//