pAAVLINK-EF1alpha-HA-5'-ABCA10

LOCUS       pAAVLINK-EF1alpha-HA-5'-ABCA10               5807 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 5807)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..5807
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..2947
                     /codon_start=1
                     /gene="ABCA10"
                     /gene_synonym="EST698739"
                     /product="ATP-binding cassette sub-family A member 10"
                     /label=ABCA10
                     /note="ATP-binding cassette A10; ATP-binding cassette, 
                     sub-family A (ABC1), member 10"
                     /db_xref="GI:1789691080"
                     /db_xref="CCDS:CCDS11684.1"
                     /db_xref="GeneID:10349"
                     /db_xref="HGNC:HGNC:30"
                     /db_xref="MIM:612508"
                     /protein_id="NP_001364250.1"
                     /translation="NKMALASFMKGRTVIGTPDEETMDIELPKKYHEMVGVIFSDTFSY
                     RLKFNWGYRIPVIKEHSEYTEHCWAMHGEIFCYLAKYWLKGFVAFQAAINAAIIEVTTN
                     HSVMEELTSVIGINMKIPPFISKGEIMNEWFHFTCLVSFSSFIYFASLNVARERGKFKK
                     LMTVMGLRESAFWLSWGLTYICFIFIMSIFMALVITSISIVFHTGFMVIFTLYSLYGLS
                     LIALAFLMSVLIRKPMLAGLAGFLFTVFWGCLGFTVLYRQLPLSLGWVLSLLSPFAFTA
                     GMAQITHLDNYLSGVIFPDPSGDSYKMIATFFILAFDTLFYLIFTLYFERVLPDKDGHG
                     DSPLFFLKSSFWSKHQNTHHEIFENEINPEHSSDDSFEPVSPEFHGKEAIRIRNVIKEY
                     NGKTGKVEALQGIFFDIYEGQITAILGHNGAGKSTLLNILSGLSVSTEGSATIYNTQLS
                     EITDMEEIRKNIGFCPQFNFQFDFLTVRENLRVFAKIKGIQPKEVEQEVKRIIMELDMQ
                     SIQDIIAKKLSGGQKRKLTLGIAILGDPQVLLLDEPTAGLDPFSRHRVWSLLKEHKVDR
                     LILFSTQFMDEADILADRKVFLSNGKLKCAGSSLFLKRKWGIGYHLSLHRNEMCDTEKI
                     TSLIKQHIPDAKLTTESEEKLVYSLPLEKTNKFPDLYSDLDKCSDQGIRNYAVSVTSLN
                     EVFLNLEGKSAIDEPDFDIGKQEKIHVTRNTGDESEMEQ"
     misc_feature    2449..2454
                     /label=SDS
     intron          2948..2988
                     /label=Splicing Donor
     misc_feature    2992..3025
                     /label=lox2272-JT1571
     repeat_region   3070..3210
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3070..3199
                     /label=AAV2 ITR
     repeat_region   3071..3211
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      3285..3740
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4022..4126
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4127..4987
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5158..5746
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctaa taagatggcc ttggcttcct ttatgaaagg
      781 aagaacagtc attgggacac cagatgaaga gaccatggat atagaacttc caaaaaaata
      841 ccatgaaatg gtgggagtta tatttagtga tactttctca tatcgcctga agtttaattg
      901 gggatataga atcccagtta taaaggagca ctctgaatac acagaacact gttgggccat
      961 gcatggtgaa attttttgtt acttggcaaa gtactggcta aaagggtttg tagcttttca
     1021 agctgcaatt aatgctgcaa ttatagaagt cacaacaaat cattctgtaa tggaggagtt
     1081 gacatcagtt attggaataa atatgaagat accacctttc atttctaagg gagaaattat
     1141 gaatgaatgg tttcatttta cttgcttagt ttctttctct tcttttatat actttgcatc
     1201 attaaatgtt gcaagggaaa gaggaaaatt taagaaactg atgacagtaa tgggtctccg
     1261 agagtcagca ttctggctct cctggggatt gacatacatt tgcttcatct tcattatgtc
     1321 catttttatg gctctggtca taacatcaat ctcaattgta tttcatactg gcttcatggt
     1381 gatattcaca ctctatagct tatatggcct ttctttgata gcattggctt tcctcatgag
     1441 tgttttaata aggaaaccta tgctcgctgg tttggctgga tttctcttca ctgtattttg
     1501 gggatgtctg ggattcactg tgttatatag acaacttcct ttatctttgg gatgggtatt
     1561 aagtcttctt agcccttttg ccttcactgc tggaatggcc cagattacac acctggataa
     1621 ttacttaagt ggtgttattt ttcctgatcc ctctggggat tcatacaaaa tgatagccac
     1681 ttttttcatt ttggcatttg atactctttt ctatttgata ttcacattat attttgagcg
     1741 agttttacct gataaagatg gccatgggga ttctccatta tttttcctta agtcctcatt
     1801 ttggtccaaa catcaaaata ctcatcatga aatctttgag aatgaaataa atcctgagca
     1861 ttcctctgat gattcttttg aaccggtgtc tccagaattc catggaaaag aagccataag
     1921 aatcagaaat gttataaaag aatataatgg aaagactgga aaagtagaag cattgcaagg
     1981 catatttttt gacatatatg aaggacagat cactgcaata cttgggcata atggagctgg
     2041 taaatcaaca ctgctaaaca ttcttagtgg attgtctgtt tctacagaag gatcagccac
     2101 tatttataat actcaactct ctgaaataac tgacatggaa gaaattagaa agaatattgg
     2161 attttgtcca cagttcaatt ttcaatttga cttcctcact gtgagagaaa acctcagggt
     2221 atttgctaaa ataaaaggga ttcagccaaa ggaagtggaa caagaggtaa aaagaattat
     2281 aatggaatta gacatgcaaa gcattcaaga cattattgct aaaaaattaa gtggtgggca
     2341 gaagagaaaa ctaacactag ggattgccat cttaggagat cctcaggttt tgctgctaga
     2401 tgaaccaact gctggattgg atcccttttc aagacaccga gtgtggagcc tcctgaagga
     2461 gcataaagta gaccgactta tcctcttcag tacccaattc atggatgagg ctgacatctt
     2521 ggctgatagg aaagtatttc tgtctaatgg gaagttgaaa tgtgcaggat catctttgtt
     2581 tctgaagcga aagtggggta ttggatatca tttaagttta cacaggaatg aaatgtgtga
     2641 cacagaaaaa atcacatccc ttattaagca gcacattcct gatgccaagt taacaacaga
     2701 aagtgaagaa aaacttgtat atagtttgcc tttggaaaaa acgaacaaat ttccagatct
     2761 ttacagtgac cttgataagt gttctgacca gggcataagg aattatgctg tttcagtgac
     2821 atctctgaat gaagtattct tgaacctaga aggaaaatca gcaattgatg aaccagattt
     2881 tgacattggg aaacaagaga aaatacatgt gacaagaaat actggagatg agtctgaaat
     2941 ggaacaggta agtatcaagg ttacaagaca ggtttaagga gaccaataag ctattattcg
     3001 tataggatac tttatacgaa gttattaagt tatggagacc ggtgtggcac gtgcggaccg
     3061 agcggccgca ggaaccccta gtgatggagt tggccactcc ctctctgcgc gctcgctcgc
     3121 tcactgaggc cgggcgacca aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag
     3181 tgagcgagcg agcgcgcagc tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc
     3241 atctgtgcgg tatttcacac cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg
     3301 cgcattaagc gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc
     3361 cttagcgccc gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc
     3421 ccgtcaagct ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct
     3481 cgaccccaaa aaacttgatt tgggtgatgg ttcacgtagt gggccatcgc cctgatagac
     3541 ggtttttcgc cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac
     3601 tggaacaaca ctcaactcta tctcgggcta ttcttttgat ttataaggga ttttgccgat
     3661 ttcggtctat tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa
     3721 aatattaacg tttacaattt tatggtgcac tctcagtaca atctgctctg atgccgcata
     3781 gttaagccag ccccgacacc cgccaacacc cgctgacgcg ccctgacggg cttgtctgct
     3841 cccggcatcc gcttacagac aagctgtgac cgtctccggg agctgcatgt gtcagaggtt
     3901 ttcaccgtca tcaccgaaac gcgcgagacg aaagggcctc gtgatacgcc tatttttata
     3961 ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc ggggaaatgt
     4021 gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc cgctcatgag
     4081 acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga gtattcaaca
     4141 tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt ttgctcaccc
     4201 agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag tgggttacat
     4261 cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag aacgttttcc
     4321 aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgta ttgacgccgg
     4381 gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg agtactcacc
     4441 agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca gtgctgccat
     4501 aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag gaccgaagga
     4561 gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc gttgggaacc
     4621 ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg tagcaatggc
     4681 aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc ggcaacaatt
     4741 aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg cccttccggc
     4801 tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg gtatcattgc
     4861 agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga cggggagtca
     4921 ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac tgattaagca
     4981 ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa aacttcattt
     5041 ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca aaatccctta
     5101 acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg
     5161 agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac cgctaccagc
     5221 ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa ctggcttcag
     5281 cagagcgcag ataccaaata ctgttcttct agtgtagccg tagttaggcc accacttcaa
     5341 gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag tggctgctgc
     5401 cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac cggataaggc
     5461 gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc gaacgaccta
     5521 caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc ccgaagggag
     5581 aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca cgagggagct
     5641 tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc tctgacttga
     5701 gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc
     5761 ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgt
//