| 质粒名称: | FU-tet-o-hKLF4 |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 19777 |
| 存储实验室: | Konrad Hochedlinger |
|
载体骨架基本信息 |
|
| 载体名称: | FUdeltaGW |
| 空载体大小: | 8400 bp |
| 完整质粒大小: | 9801 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
| 拷贝数: | 高拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | Klf4 |
| 物种来源: | 人源 |
| 插入大小: | 1425bp |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | EcoRI(未破坏) |
| 3’克隆位点: | EcoRI (未破坏) |
| 5’测序引物 | CMV-F |
|
文献引用方法 |
|
| 材料和方法部分: | FU-tet-o-hKLF4 was a gift from Konrad Hochedlinger (Addgene plasmid # 19777 ; http://n2t.net/addgene:19777 ; RRID:Addgene_19777) |
| 参考文献部分: | A high-efficiency system for the generation and study of human induced pluripotent stem cells. Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger K. Cell Stem Cell. 2008 Sep 11. 3(3):340-5. 10.1016/j.stem.2008.08.003 PubMed 18786420 |
买家导航
FU-tet-o-hKLF4载体质粒基本信息
FU-tet-o-hKLF4质粒图谱载体图谱和FU-tet-o-hKLF4载体序列质粒序列多克隆位点信息
FU-tet-o-hKLF4质粒载体简介
FU-tet-o-hKLF4质粒序列载体序列
LOCUS FU-tet-o-hKLF4 9801 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS FU-tet-o-hKLF4
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9801)
AUTHORS Maherali N, Ahfeldt T, Rigamonti A, Utikal J, Cowan C, Hochedlinger
K
TITLE A high-efficiency system for the generation and study of human
induced pluripotent stem cells.
JOURNAL Cell Stem Cell. 2008 Sep 11. 3(3):340-5.
PUBMED 18786420
REFERENCE 2 (bases 1 to 9801)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9801
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind complement(47..66)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
enhancer 238..617
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 619..817
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 772..792
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
LTR 835..1015
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 1062..1187
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1680..1913
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 2098..2142
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 2440..2557
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
protein_bind 2610..2880
/label=tetracycline response element
/bound_moiety="tetracycline repressor TetR"
/note="contains seven copies of the tetracycline operator
tetO"
promoter 2913..2950
/label=minimal CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 2947..2971
/label=LNCX
/note="Human CMV promoter, forward primer"
regulatory 3050..3059
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
misc_feature 4493..5081
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4546..4566)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4964..4975)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(5084..5100)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(5085..5101)
/label=pBluescriptKS
/note="For pBluescript vector"
LTR 5606..5786
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
primer_bind complement(5812..5829)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5818..6042
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 6088..6516
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(6175..6194)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 6385..6406
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(6525..6545)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
promoter 6530..6859
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 6710..6845
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 6772..6791
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
promoter 6907..6954
/label=EM7 promoter
/note="synthetic bacterial promoter "
CDS 6973..7347
/codon_start=1
/gene="Sh ble from Streptoalloteichus hindustanus"
/product="antibiotic-binding protein"
/label=BleoR
/note="confers resistance to bleomycin, phleomycin, and
Zeocin(TM)"
/translation="MAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRDD
VTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWGR
EFALRDPAGNCVHFVAEEQD"
polyA_signal 7477..7598
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(7514..7533)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 7568..7587
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
primer_bind complement(7647..7663)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(7647..7663)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(7660..7682)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 7671..7687
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(7695..7725)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7740..7761
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(7878..7895)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(8049..8637)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(8129..8148)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(8808..9668)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 9431..9450
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(9669..9773)
/gene="bla"
/label=AmpR promoter
ORIGIN
1 gtcgacggat cgggagatct cccgatcccc tatggtgcac tctcagtaca atctgctctg
61 atgccgcata gttaagccag tatctgctcc ctgcttgtgt gttggaggtc gctgagtagt
121 gcgcgagcaa aatttaagct acaacaaggc aaggcttgac cgacaattgc atgaagaatc
181 tgcttagggt taggcgtttt gcgctgcttc gcgatgtacg ggccagatat acgcgttgac
241 attgattatt gactagttat taatagtaat caattacggg gtcattagtt catagcccat
301 atatggagtt ccgcgttaca taacttacgg taaatggccc gcctggctga ccgcccaacg
361 acccccgccc attgacgtca ataatgacgt atgttcccat agtaacgcca atagggactt
421 tccattgacg tcaatgggtg gagtatttac ggtaaactgc ccacttggca gtacatcaag
481 tgtatcatat gccaagtacg ccccctattg acgtcaatga cggtaaatgg cccgcctggc
541 attatgccca gtacatgacc ttatgggact ttcctacttg gcagtacatc tacgtattag
601 tcatcgctat taccatggtg atgcggtttt ggcagtacat caatgggcgt ggatagcggt
661 ttgactcacg gggatttcca agtctccacc ccattgacgt caatgggagt ttgttttggc
721 accaaaatca acgggacttt ccaaaatgtc gtaacaactc cgccccattg acgcaaatgg
781 gcggtaggcg tgtacggtgg gaggtctata taagcagcgc gttttgcctg tactgggtct
841 ctctggttag accagatctg agcctgggag ctctctggct aactagggaa cccactgctt
901 aagcctcaat aaagcttgcc ttgagtgctt caagtagtgt gtgcccgtct gttgtgtgac
961 tctggtaact agagatccct cagacccttt tagtcagtgt ggaaaatctc tagcagtggc
1021 gcccgaacag ggacttgaaa gcgaaaggga aaccagagga gctctctcga cgcaggactc
1081 ggcttgctga agcgcgcacg gcaagaggcg aggggcggcg actggtgagt acgccaaaaa
1141 ttttgactag cggaggctag aaggagagag atgggtgcga gagcgtcagt attaagcggg
1201 ggagaattag atcgcgatgg gaaaaaattc ggttaaggcc agggggaaag aaaaaatata
1261 aattaaaaca tatagtatgg gcaagcaggg agctagaacg attcgcagtt aatcctggcc
1321 tgttagaaac atcagaaggc tgtagacaaa tactgggaca gctacaacca tcccttcaga
1381 caggatcaga agaacttaga tcattatata atacagtagc aaccctctat tgtgtgcatc
1441 aaaggataga gataaaagac accaaggaag ctttagacaa gatagaggaa gagcaaaaca
1501 aaagtaagac caccgcacag caagcggccg ctgatcttca gacctggagg aggagatatg
1561 agggacaatt ggagaagtga attatataaa tataaagtag taaaaattga accattagga
1621 gtagcaccca ccaaggcaaa gagaagagtg gtgcagagag aaaaaagagc agtgggaata
1681 ggagctttgt tccttgggtt cttgggagca gcaggaagca ctatgggcgc agcgtcaatg
1741 acgctgacgg tacaggccag acaattattg tctggtatag tgcagcagca gaacaatttg
1801 ctgagggcta ttgaggcgca acagcatctg ttgcaactca cagtctgggg catcaagcag
1861 ctccaggcaa gaatcctggc tgtggaaaga tacctaaagg atcaacagct cctggggatt
1921 tggggttgct ctggaaaact catttgcacc actgctgtgc cttggaatgc tagttggagt
1981 aataaatctc tggaacagat ttggaatcac acgacctgga tggagtggga cagagaaatt
2041 aacaattaca caagcttaat acactcctta attgaagaat cgcaaaacca gcaagaaaag
2101 aatgaacaag aattattgga attagataaa tgggcaagtt tgtggaattg gtttaacata
2161 acaaattggc tgtggtatat aaaattattc ataatgatag taggaggctt ggtaggttta
2221 agaatagttt ttgctgtact ttctatagtg aatagagtta ggcagggata ttcaccatta
2281 tcgtttcaga cccacctccc aaccccgagg ggacccgaca ggcccgaagg aatagaagaa
2341 gaaggtggag agagagacag agacagatcc attcgattag tgaacggatc ggcactgcgt
2401 gcgccaattc tgcagacaaa tggcagtatt catccacaat tttaaaagaa aaggggggat
2461 tggggggtac agtgcagggg aaagaatagt agacataata gcaacagaca tacaaactaa
2521 agaattacaa aaacaaatta caaaaattca aaattttcgg gtttattaca gggacagcag
2581 agatccagtt tggttatcga gtttaccact ccctatcagt gatagagaaa agtgaaagtc
2641 gagtttacca ctccctatca gtgatagaga aaagtgaaag tcgagtttac cactccctat
2701 cagtgataga gaaaagtgaa agtcgagttt accactccct atcagtgata gagaaaagtg
2761 aaagtcgagt ttaccactcc ctatcagtga tagagaaaag tgaaagtcga gtttaccact
2821 ccctatcagt gatagagaaa agtgaaagtc gagtttacca ctccctatca gtgatagaga
2881 aaagtgaaag tcgagctcgg tacccgggtc gagtaggcgt gtacggtggg aggcctatat
2941 aagcagagct cgtttagtga accgtcagat cgcctggaga cgccatccac gctgttttga
3001 cctccataga agacaccggg accgatccag cctccgcggc cccgaattcg ccaccatggc
3061 tgtcagcgac gcgctgctcc catctttctc cacgttcgcg tctggcccgg cgggaaggga
3121 gaagacactg cgtcaagcag gtgccccgaa taaccgctgg cgggaggagc tctcccacat
3181 gaagcgactt cccccagtgc ttcccggccg cccctatgac ctggcggcgg cgaccgtggc
3241 cacagacctg gagagcggcg gagccggtgc ggcttgcggc ggtagcaacc tggcgcccct
3301 acctcggaga gagaccgagg agttcaacga tctcctggac ctggacttta ttctctccaa
3361 ttcgctgacc catcctccgg agtcagtggc cgccaccgtg tcctcgtcag cgtcagcctc
3421 ctcttcgtcg tcgccgtcga gcagcggccc tgccagcgcg ccctccacct gcagcttcac
3481 ctatccgatc cgggccggga acgacccggg cgtggcgccg ggcggcacgg gcggaggcct
3541 cctctatggc agggagtccg ctccccctcc gacggctccc ttcaacctgg cggacatcaa
3601 cgacgtgagc ccctcgggcg gcttcgtggc cgagctcctg cggccagaat tggacccggt
3661 gtacattccg ccgcagcagc cgcagccgcc aggtggcggg ctgatgggca agttcgtgct
3721 gaaggcgtcg ctgagcgccc ctggcagcga gtacggcagc ccgtcggtca tcagcgtcag
3781 caaaggcagc cctgacggca gccacccggt ggtggtggcg ccctacaacg gcgggccgcc
3841 gcgcacgtgc cccaagatca agcaggaggc ggtctcttcg tgcacccact tgggcgctgg
3901 accccctctc agcaatggcc accggccggc tgcacacgac ttccccctgg ggcggcagct
3961 ccccagcagg actaccccga ccctgggtct tgaggaagtg ctgagcagca gggactgtca
4021 ccctgccctg ccgcttcctc ccggcttcca tccccacccg gggcccaatt acccatcctt
4081 cctgcccgat cagatgcagc cgcaagtccc gccgctccat taccaagagc tcatgccacc
4141 cggttcctgc atgccagagg agcccaagcc aaagagggga agacgatcgt ggccccggaa
4201 aaggaccgcc acccacactt gtgattacgc gggctgcggc aaaacctaca caaagagttc
4261 ccatctcaag gcacacctgc gaacccacac aggtgagaaa ccttaccact gtgactggga
4321 cggctgtgga tggaaattcg cccgctcaga tgaactgacc aggcactacc gtaaacacac
4381 ggggcaccgc ccgttccagt gccaaaaatg cgaccgagca ttttccaggt cggaccacct
4441 cgccttacac atgaagaggc atttttaaga attcgatatc aagcttatcg ataatcaacc
4501 tctggattac aaaatttgtg aaagattgac tggtattctt aactatgttg ctccttttac
4561 gctatgtgga tacgctgctt taatgccttt gtatcatgct attgcttccc gtatggcttt
4621 cattttctcc tccttgtata aatcctggtt gctgtctctt tatgaggagt tgtggcccgt
4681 tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac gcaaccccca ctggttgggg
4741 cattgccacc acctgtcagc tcctttccgg gactttcgct ttccccctcc ctattgccac
4801 ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc tgttgggcac
4861 tgacaattcc gtggtgttgt cggggaaatc atcgtccttt ccttggctgc tcgcctgtgt
4921 tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc ccttcggccc tcaatccagc
4981 ggaccttcct tcccgcggcc tgctgccggc tctgcggcct cttccgcgtc ttcgccttcg
5041 ccctcagacg agtcggatct ccctttgggc cgcctccccg catcgatacc gtcgacctcg
5101 agacctagaa aaacatggag caatcacaag tagcaataca gcagctacca atgctgattg
5161 tgcctggcta gaagcacaag aggaggagga ggtgggtttt ccagtcacac ctcaggtacc
5221 tttaagacca atgacttaca aggcagctgt agatcttagc cactttttaa aagaaaaggg
5281 gggactggaa gggctaattc actcccaacg aagacaagat atccttgatc tgtggatcta
5341 ccacacacaa ggctacttcc ctgattggca gaactacaca ccagggccag ggatcagata
5401 tccactgacc tttggatggt gctacaagct agtaccagtt gagcaagaga aggtagaaga
5461 agccaatgaa ggagagaaca cccgcttgtt acaccctgtg agcctgcatg ggatggatga
5521 cccggagaga gaagtattag agtggaggtt tgacagccgc ctagcatttc atcacatggc
5581 ccgagagctg catccggact gtactgggtc tctctggtta gaccagatct gagcctggga
5641 gctctctggc taactaggga acccactgct taagcctcaa taaagcttgc cttgagtgct
5701 tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac tagagatccc tcagaccctt
5761 ttagtcagtg tggaaaatct ctagcagggc ccgtttaaac ccgctgatca gcctcgactg
5821 tgccttctag ttgccagcca tctgttgttt gcccctcccc cgtgccttcc ttgaccctgg
5881 aaggtgccac tcccactgtc ctttcctaat aaaatgagga aattgcatcg cattgtctga
5941 gtaggtgtca ttctattctg gggggtgggg tggggcagga cagcaagggg gaggattggg
6001 aagacaatag caggcatgct ggggatgcgg tgggctctat ggcttctgag gcggaaagaa
6061 ccagctgggg ctctaggggg tatccccacg cgccctgtag cggcgcatta agcgcggcgg
6121 gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt
6181 tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc
6241 gggggctccc tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg
6301 attagggtga tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga
6361 cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc
6421 ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa
6481 aaaatgagct gatttaacaa aaatttaacg cgaattaatt ctgtggaatg tgtgtcagtt
6541 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
6601 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
6661 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
6721 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
6781 agaggccgag gccgcctctg cctctgagct attccagaag tagtgaggag gcttttttgg
6841 aggcctaggc ttttgcaaaa agctcccggg agcttgtata tccattttcg gatctgatca
6901 gcacgtgttg acaattaatc atcggcatag tatatcggca tagtataata cgacaaggtg
6961 aggaactaaa ccatggccaa gttgaccagt gccgttccgg tgctcaccgc gcgcgacgtc
7021 gccggagcgg tcgagttctg gaccgaccgg ctcgggttct cccgggactt cgtggaggac
7081 gacttcgccg gtgtggtccg ggacgacgtg accctgttca tcagcgcggt ccaggaccag
7141 gtggtgccgg acaacaccct ggcctgggtg tgggtgcgcg gcctggacga gctgtacgcc
7201 gagtggtcgg aggtcgtgtc cacgaacttc cgggacgcct ccgggccggc catgaccgag
7261 atcggcgagc agccgtgggg gcgggagttc gccctgcgcg acccggccgg caactgcgtg
7321 cacttcgtgg ccgaggagca ggactgacac gtgctacgag atttcgattc caccgccgcc
7381 ttctatgaaa ggttgggctt cggaatcgtt ttccgggacg ccggctggat gatcctccag
7441 cgcggggatc tcatgctgga gttcttcgcc caccccaact tgtttattgc agcttataat
7501 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
7561 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctgtat accgtcgacc
7621 tctagctaga gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
7681 ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
7741 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
7801 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
7861 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
7921 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
7981 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
8041 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
8101 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
8161 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
8221 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
8281 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
8341 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
8401 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
8461 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
8521 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
8581 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
8641 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
8701 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
8761 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
8821 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
8881 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
8941 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
9001 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
9061 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
9121 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
9181 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
9241 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
9301 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
9361 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
9421 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
9481 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
9541 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
9601 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
9661 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
9721 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
9781 ccccgaaaag tgccacctga c
//
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FU-tet-o-hKLF4质粒载体应用举例
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