pSL1533

LOCUS       pSL1533             7209 bp DNA     circular SYN 11-FEB-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7209)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 30, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7209
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR (alternate)
                     /note="Functional equivalent of wild-type AAV2 ITR"
     primer_bind     123..164
                     /label=oSCL1800
     misc_feature    143..725
                     /label=CMV
     primer_bind     complement(699..730)
                     /label=OXB018
     primer_bind     706..749
                     /label=oSCL1791
     primer_bind     709..729
                     /label=oSCL1013
     misc_feature    731..732
                     /label=linker
     primer_bind     complement(734..748)
                     /label=Oscl28-2
     primer_bind     complement(735..748)
                     /label=8x R
     misc_feature    749..761
                     /label=linker
     misc_feature    767..785
                     /label=MS2 box
     misc_feature    791..800
                     /label=linker
     misc_feature    806..824
                     /label=MS2 box
     misc_feature    830..839
                     /label=linker
     misc_feature    830..839
                     /label=linker
     misc_feature    838..868
                     /label=hairpin
     misc_feature    844..862
                     /label=MS2 box
     misc_feature    867..879
                     /label=linker
     misc_feature    878..908
                     /label=hairpin
     misc_feature    884..902
                     /label=MS2 box
     misc_feature    912..917
                     /label=linker
     primer_bind     922..943
                     /label=oSCL1848
     misc_feature    926..941
                     /label=linker
     misc_feature    968..980
                     /label=linker
     misc_feature    986..1004
                     /label=MS2 box
     misc_feature    1010..1019
                     /label=linker
     misc_feature    1025..1043
                     /label=MS2 box
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1049..1058
                     /label=linker
     misc_feature    1057..1087
                     /label=hairpin
     misc_feature    1063..1081
                     /label=MS2 box
     misc_feature    1086..1098
                     /label=linker
     misc_feature    1097..1127
                     /label=hairpin
     misc_feature    1103..1121
                     /label=MS2 box
     misc_feature    1131..1136
                     /label=linker
     primer_bind     1141..1166
                     /label=oSCL1848
     misc_feature    1145..1160
                     /label=linker
     primer_bind     1161..1195
                     /label=oSCL2527
     misc_feature    1173..2855
                     /label=nVEGFi
     CDS             1173..1250
                     /codon_start=1
                     /label=Signal peptide
                     /translation="MVSYWDTGVLLCALLSCLLLTGSSSG"
     CDS             1251..1541
                     /codon_start=1
                     /label=VEGFR1 IgG1
                     /translation="SKLKDPELSLKGTQHIMQAGQTLHLQCRGEAAHKWSLPEMVSKES
                     ERLSITKSACGRNGKQFCSTLTLNTAQANHTGFYSCKYLAVPTSKKKETESA"
     CDS             1542..1805
                     /codon_start=1
                     /label=VEGFR1 IgG2
                     /translation="IYIFISDTGRPFVEMYSEIPEIIHMTEGRELVIPCRVTSPNITVT
                     LKKFPLDTLIPDGKRIIWDSRKGFIISNATYKEIGLLTCEATV"
     CDS             1806..2171
                     /codon_start=1
                     /label=VEGFR2 IgG3
                     /translation="NGHLYKTNYLTHRQTNTIIDVVLSPSHGIELSVGEKLVLNCTART
                     ELNVGIDFNWEYPSSKHQHKKLVNRDLKTQSGSEMKKFLSTLTIDGVTRSDQGLYTCAA
                     SSGLMTKKNSTFVRVHEK"
     CDS             2172..2852
                     /codon_start=1
                     /label= IgG FC
                     /translation="DKTHTCPPCPAPELLGGPSVFLFPPKPKDTLMISRTPEVTCVVVD
                     VSHEDPEVKFNWYVDGVEVHNAKTKPREEQYNSTYRVVSVLTVLHQDWLNGKEYKCKVS
                     NKALPAPIEKTISKAKGQPREPQVYTLPPSRDELTKNQVSLTCLVKGFYPSDIAVEWES
                     NGQPENNYKTTPPVLDSDGSFFLYSKLTVDKSRWQQGNVFSCSVMHEALHNHYTQKSLS
                     LSPGK"
     primer_bind     complement(2211..2221)
                     /label=oSCL2412
     primer_bind     complement(2836..2862)
                     /label=oSCL2528
     primer_bind     2855..2890
                     /label=oSCL1795
     primer_bind     complement(2855..2875)
                     /label=oSCL1849
     polyA_signal    2862..2983
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     primer_bind     2862..2890
                     /label=OXB007
     primer_bind     complement(2873..2892)
                     /label=EBV-rev
                     /note="SV40 polyA terminator, reverse primer"
     primer_bind     2886..2906
                     /label=PSG5-R(SV40-R)
     primer_bind     2927..2946
                     /label=SV40pA-R
                     /note="SV40 polyA, reverse primer"
     primer_bind     complement(2954..2983)
                     /label=OXB016
     primer_bind     complement(2958..2983)
                     /label=OXB008
     primer_bind     complement(2967..2989)
                     /label=oSCL1801
     primer_bind     complement(2967..2983)
                     /label=oSCL1174
     primer_bind     complement(2967..2983)
                     /label=oSCL1796
     misc_feature    2988..3575
                     /label=CMV
     primer_bind     2988..3009
                     /label=oSCL1800
     primer_bind     complement(3544..3575)
                     /label=OXB018
     primer_bind     3551..3575
                     /label=oSCL1791
     primer_bind     3554..3574
                     /label=oSCL1013
     primer_bind     3576..3595
                     /label=T7
     primer_bind     3582..3631
                     /label=oSCL2413
     primer_bind     3605..3631
                     /label=oSCL2381
     CDS             3614..3766
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGIAEWISSNSRSQAYKVT
                     CSVRQS"
     CDS             3767..4177
                     /codon_start=1
                     /label=LOV2
                     /translation="LERIEKNFVITDPRLPDNPIIFASDSFLQLTEYSREEILGRNCRF
                     LQGPETDRATVRKIRDAIDNQTEVTVQLINYTKSGKKFWNLFHLQPMRDQKGDVQYFIG
                     VQLDGTEHVRDAAEREGVMLIKKTAENIDEAAG"
     primer_bind     3771..3813
                     /label=oSCL2408
     primer_bind     complement(3771..3790)
                     /label=oSCL2407
     CDS             3791..3793
                     /codon_start=1
                     /label=V416I
                     /translation="V"
     primer_bind     4107..4149
                     /label=oSCL2410
     primer_bind     complement(4107..4126)
                     /label=oSCL2409
     CDS             4127..4129
                     /codon_start=1
                     /label=G528A
                     /translation="G"
     primer_bind     4137..4180
                     /label=oSCL2412
     primer_bind     complement(4137..4156)
                     /label=oSCL2411
     CDS             4157..4159
                     /codon_start=1
                     /label=N538E
                     /translation="N"
     CDS             4178..4405
                     /codon_start=1
                     /label=MS2-N55K
                     /translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
                     SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
     primer_bind     complement(4386..4428)
                     /label=oSCL2415
     primer_bind     complement(4386..4408)
                     /label=oSCL2383
     primer_bind     complement(4386..4405)
                     /label=oSCL2382
     primer_bind     4386..4405
                     /label=oSCL2384
     misc_feature    4415..4463
                     /label=HSV TK poly(A) signal
     primer_bind     complement(4439..4471)
                     /label=oSCL1799
     repeat_region   4472..4612
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     repeat_region   4472..4601
                     /label=AAV2 ITR
     rep_origin      4687..5142
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(4774..4793)
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     4984..5005
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     complement(5159..5178)
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     5278..5300
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
     primer_bind     complement(5338..5356)
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     promoter        5424..5528
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5529..6389
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(5747..5766)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      6560..7148
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     7049..7068
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggcctcta gagttgacat tgattattga ctagttatta atagtaatca
      181 attacggggt cattagttca tagcccatat atggagttcc gcgttacata acttacggta
      241 aatggcccgc ctggctgacc gcccaacgac ccccgcccat tgacgtcaat aatgacgtat
      301 gttcccatag taacgccaat agggactttc cattgacgtc aatgggtgga gtatttacgg
      361 taaactgccc acttggcagt acatcaagtg tatcatatgc caagtacgcc ccctattgac
      421 gtcaatgacg gtaaatggcc cgcctggcat tatgcccagt acatgacctt atgggacttt
      481 cctacttggc agtacatcta cgtattagtc atcgctatta ccatggtgat gcggttttgg
      541 cagtacatca atgggcgtgg atagcggttt gactcacggg gatttccaag tctccacccc
      601 attgacgtca atgggagttt gttttggcac caaaatcaac gggactttcc aaaatgtcgt
      661 aacaactccg ccccattgac gcaaatgggc ggtaggcgtg tacggtggga ggtctatata
      721 agcagagctc cttgaaccgg taagaattgc aacaaacaaa tcaccaccgt gaggatcacc
      781 cacggtggtg aaagccttag cctagccgtg aggatcaccc acggtaggca caagaaattc
      841 tacccgtgag gatcacccac gggtagaatc acaaagacta cagccgtgag gatcacccac
      901 ggctgtagac tcaaacttaa aaaggatatc ttcagcaaac tgatcggaga cgagtccgtc
      961 tcaaattgca acaaacaaat caccaccgtg aggatcaccc acggtggtga aagccttagc
     1021 ctagccgtga ggatcaccca cggtaggcac aagaaattct acccgtgagg atcacccacg
     1081 ggtagaatca caaagactac agccgtgagg atcacccacg gctgtagact caaacttaaa
     1141 aaggatatct tcagcaaact gaattcgcca ccatggtttc ttactgggat accggcgtgc
     1201 tgctgtgtgc cctgctgtct tgtctgctgc tgaccggctc tagcagcggc agcaagctga
     1261 aggatcccga gctgagcctg aagggcaccc agcatattat gcaggccggc cagacactgc
     1321 acctccagtg tagaggcgaa gccgctcaca aatggtccct gcctgagatg gtgtccaaag
     1381 agagcgagcg gctgagcatc accaagagcg cctgtggcag aaacggcaag cagttctgca
     1441 gcaccctgac actgaatact gcccaggcca accacaccgg cttctacagc tgcaagtatc
     1501 tggccgtgcc taccagcaag aagaaagaaa ccgagagcgc catctacatc ttcatctctg
     1561 ataccggcag acccttcgtg gaaatgtaca gcgagatccc cgagatcatc cacatgaccg
     1621 agggcagaga gctggtcatc ccctgcagag tgacaagccc caacatcacc gtgactctga
     1681 agaagttccc tctggacaca ctgatccccg acggcaagag aatcatctgg gacagccgga
     1741 agggcttcat catcagcaac gccacctaca aagagatcgg cctgctgacc tgtgaagcca
     1801 ccgtgaatgg ccacctgtac aagaccaact acctgacaca cagacagacc aacaccatca
     1861 tcgacgtggt gctgagccct agccacggca ttgaactgtc tgtgggcgag aagctggtgc
     1921 tgaactgtac cgccagaacc gagctgaacg tgggcatcga cttcaactgg gagtacccca
     1981 gcagcaagca ccagcacaag aaactggtca accgggacct gaaaacccag agcggcagcg
     2041 agatgaagaa attcctgagc accctgacca tcgacggcgt gaccagatct gaccagggcc
     2101 tgtatacttg tgccgccagc tctggcctga tgaccaagaa aaacagcacc ttcgtgcggg
     2161 tgcacgagaa ggacaagacc cacacctgtc ctccatgtcc tgctccagaa ctgctcggcg
     2221 gaccttccgt gttcctgttt cctccaaagc ctaaggacac cctgatgatc agcagaaccc
     2281 ctgaagtgac ctgcgtggtg gtggatgtgt cccacgagga tcccgaagtg aagttcaatt
     2341 ggtacgtgga cggcgtggaa gtgcacaacg ccaagaccaa gcctagagag gaacagtaca
     2401 atagcaccta cagagtggtg tccgtgctga ccgtgctgca ccaggattgg ctgaacggca
     2461 aagagtacaa gtgcaaggtg tccaacaagg ccctgcctgc tcctatcgag aaaaccatct
     2521 ccaaggccaa gggccagcct agggaacccc aggtttacac actgcctcca agcagggacg
     2581 agctgacaaa gaaccaggtg tccctgacct gcctggtcaa gggcttctac ccttccgata
     2641 tcgccgtgga atgggagagc aatggccagc ctgagaacaa ctacaagaca acccctcctg
     2701 tgctggacag cgacggctca ttcttcctgt acagcaagct gacagtggac aagagcagat
     2761 ggcagcaggg aaacgtgttc agctgcagcg tgatgcacga ggccctgcac aaccactaca
     2821 cccagaagtc cctgagcctg tctcctggca aataactcga gtaagataca ttgatgagtt
     2881 tggacaaacc acaactagaa tgcagtgaaa aaaatgcttt atttgtgaaa tttgtgatgc
     2941 tattgcttta tttgtaacca ttataagctg caataaacaa gttacgcgtt gacattgatt
     3001 attgactagt tattaatagt aatcaattac ggggtcatta gttcatagcc catatatgga
     3061 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
     3121 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
     3181 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
     3241 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
     3301 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
     3361 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
     3421 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
     3481 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
     3541 gcgtgtacgg tgggaggtct atataagcag agctctaata cgactcacta tagggaagct
     3601 ttaagccacc atggctagca acttcacaca gttcgtgctg gtggacaacg gcggcaccgg
     3661 cgacgtgacc gtggccccta gcaacttcgc caacggcatc gccgagtgga tcagcagcaa
     3721 cagcagaagc caagcctaca aggtgacctg cagcgtgaga cagagcctgg agagaatcga
     3781 gaagaacttc gtgatcaccg accctagact gcccgacaac cccatcatct tcgctagcga
     3841 cagcttcctg cagctgaccg agtacagcag agaggagatc ctgggcagaa actgcagatt
     3901 cctgcaaggc cccgagaccg acagagccac cgtgagaaag atcagagacg ccatcgacaa
     3961 tcagaccgag gtgaccgtgc agctgatcaa ctacaccaag agcggcaaga agttctggaa
     4021 cctgttccac ctgcagccca tgagagatca gaagggcgac gtgcagtact tcatcggcgt
     4081 gcagctggac ggcaccgagc acgtgagaga cgccgccgag agagagggcg tgatgctgat
     4141 caagaagacc gccgagaaca tcgacgaggc cgccgggcag aagagaaagt acaccatcaa
     4201 agtcgaggtg cccaaggtgg ccacccaaac cgtgggcggc gtggagctgc ccgtggccgc
     4261 ctggagaagc tacctgaaca tggagctgac catccccatc ttcgccacca acagcgactg
     4321 cgagctgatc gtgaaggcca tgcaaggcct gctgaaggac ggcaacccca ttcctagcgc
     4381 catcgctgct aacagcggca tttattaaca attgcggcaa taaaaagaca gaataaaacg
     4441 cacgggtgtt gggtcgtttg ttcgcggccg caggaacccc tagtgatgga gttggccact
     4501 ccctctctgc gcgctcgctc gctcactgag gccgggcgac caaaggtcgc ccgacgcccg
     4561 ggctttgccc gggcggcctc agtgagcgag cgagcgcgca gctgcctgca ggggcgcctg
     4621 atgcggtatt ttctccttac gcatctgtgc ggtatttcac accgcatacg tcaaagcaac
     4681 catagtacgc gccctgtagc ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg
     4741 tgaccgctac acttgccagc gccttagcgc ccgctccttt cgctttcttc ccttcctttc
     4801 tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg ggggctccct ttagggttcc
     4861 gatttagtgc tttacggcac ctcgacccca aaaaacttga tttgggtgat ggttcacgta
     4921 gtgggccatc gccctgatag acggtttttc gccctttgac gttggagtcc acgttcttta
     4981 atagtggact cttgttccaa actggaacaa cactcaactc tatctcgggc tattcttttg
     5041 atttataagg gattttgccg atttcggtct attggttaaa aaatgagctg atttaacaaa
     5101 aatttaacgc gaattttaac aaaatattaa cgtttacaat tttatggtgc actctcagta
     5161 caatctgctc tgatgccgca tagttaagcc agccccgaca cccgccaaca cccgctgacg
     5221 cgccctgacg ggcttgtctg ctcccggcat ccgcttacag acaagctgtg accgtctccg
     5281 ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa acgcgcgaga cgaaagggcc
     5341 tcgtgatacg cctattttta taggttaatg tcatgataat aatggtttct tagacgtcag
     5401 gtggcacttt tcggggaaat gtgcgcggaa cccctatttg tttatttttc taaatacatt
     5461 caaatatgta tccgctcatg agacaataac cctgataaat gcttcaataa tattgaaaaa
     5521 ggaagagtat gagtattcaa catttccgtg tcgcccttat tccctttttt gcggcatttt
     5581 gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt aaaagatgct gaagatcagt
     5641 tgggtgcacg agtgggttac atcgaactgg atctcaacag cggtaagatc cttgagagtt
     5701 ttcgccccga agaacgtttt ccaatgatga gcacttttaa agttctgcta tgtggcgcgg
     5761 tattatcccg tattgacgcc gggcaagagc aactcggtcg ccgcatacac tattctcaga
     5821 atgacttggt tgagtactca ccagtcacag aaaagcatct tacggatggc atgacagtaa
     5881 gagaattatg cagtgctgcc ataaccatga gtgataacac tgcggccaac ttacttctga
     5941 caacgatcgg aggaccgaag gagctaaccg cttttttgca caacatgggg gatcatgtaa
     6001 ctcgccttga tcgttgggaa ccggagctga atgaagccat accaaacgac gagcgtgaca
     6061 ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact attaactggc gaactactta
     6121 ctctagcttc ccggcaacaa ttaatagact ggatggaggc ggataaagtt gcaggaccac
     6181 ttctgcgctc ggcccttccg gctggctggt ttattgctga taaatctgga gccggtgagc
     6241 gtggaagccg cggtatcatt gcagcactgg ggccagatgg taagccctcc cgtatcgtag
     6301 ttatctacac gacggggagt caggcaacta tggatgaacg aaatagacag atcgctgaga
     6361 taggtgcctc actgattaag cattggtaac tgtcagacca agtttactca tatatacttt
     6421 agattgattt aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata
     6481 atctcatgac caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag
     6541 aaaagatcaa aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa
     6601 caaaaaaacc accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt
     6661 ttccgaaggt aactggcttc agcagagcgc agataccaaa tactgttctt ctagtgtagc
     6721 cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa
     6781 tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa
     6841 gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc
     6901 ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa
     6961 gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa
     7021 caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg
     7081 ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc
     7141 tatggaaaaa cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg
     7201 ctcacatgt
//