| 质粒名称: | pAAV-CAG-CreiLOV-P2A-FusionRed |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000234 |
| 产品用途: | Expresses CreiLOV and FusionRed in mammalian cells |
| 存储实验室: | KIRYL PIATKEVICH Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
CAG
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5a，TOP10 |
|
插入基因信息 |
|
| 插入基因名称: | CreiLOV, FusionRed |
| 物种来源: | Chlamydomonas reinhardtii |
| 密码子优化: | 哺乳动物表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Enhanced small green fluorescent proteins as a multisensing platform for biosensor development
Liang G., Lai C., Yue Z., Zhang H., Li D., Chen Z., Lu X., Tao L., Subach F.V., Piatkevich K.D.
Front. Bioeng. Biotechnol. 10, 1039317 (2022)
|
pAAV-CAG-CreiLOV-P2A-FusionRed
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAV-CAG-CreiLOV-P2A-FusionRed载体质粒基本信息
pAAV-CAG-CreiLOV-P2A-FusionRed质粒图谱载体图谱和pAAV-CAG-CreiLOV-P2A-FusionRed载体序列质粒序列多克隆位点信息
pAAV-CAG-CreiLOV-P2A-FusionRed质粒载体简介
pAAV-CAG-CreiLOV-P2A-FusionRed质粒序列载体序列
LOCUS pAAV-CAG-CreiLOV-P2A-FusionRed 5742 bp DNA circular SYN 26-MAY-2023
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5742)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Jul 1, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..5742
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 279..556
/label=chicken beta-actin promoter
misc_feature 977..982
/label=Kozak sequence
misc_feature 983..1339
/label=CreiLOV
CDS 1364..1420
/codon_start=1
/product="2A peptide from porcine teschovirus-1
polyprotein"
/label=P2A
/note="Eukaryotic ribosomes fail to insert a peptide bond
between the Gly and Pro residues, yielding separate
polypeptides."
/translation="ATNFSLLKQAGDVEENPGP"
CDS 1433..2128
/codon_start=1
/product="monomeric red fluorescent protein optimized for
use as a fusion tag (Shemiakina et al., 2012)"
/label=FusionRed
/note="mammalian codon-optimized"
/translation="MVSELIKENMPMKLYMEGTVNNHHFKCTSEGEGKPYEGTQTMRIK
VVEGGPLPFAFDILATSFMYGSRTFIKHPPGIPDFFKQSFPEGFTWERVTTYEDGGVLT
ATQDTSLQDGCLIYNVKVRGVNFPANGPVMQKKTLGWEASTETMYPADGGLEGACDMAL
KLVGGGHLICNLETTYRSKKPATNLKMPGVYNVDHRLERIKEADDETYVEQHEVAVARY
STGGAGDGGK"
misc_feature 2156..2744
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(2627..2638)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
polyA_signal 2787..2908
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin complement(3306..3894)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(4065..4925)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(4926..5030)
/gene="bla"
/label=AmpR promoter
rep_origin complement(5056..5511)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
repeat_region 5583..5712
/label=AAV2 ITR
ORIGIN
1 tctacgtagc catgctctag gaagagtacc attgacgtca ataatgacgt atgttcccat
61 agtaacgcca atagggactt tccattgacg tcaatgggtg gagtatttac ggtaaactgc
121 ccacttggca gtacatcaag tgtatcatat gccaagtacg ccccctattg acgtcaatga
181 cggtaaatgg cccgcctggc attatgccca gtacatgacc ttatgggact ttcctacttg
241 gcagtacatc tacgtattag tcatcgctat taccatggtc gaggtgagcc ccacgttctg
301 cttcactctc cccatctccc ccccctcccc acccccaatt ttgtatttat ttatttttta
361 attattttgt gcagcgatgg gggcgggggg gggggggggg cgcgcgccag gcggggcggg
421 gcggggcgag gggcggggcg gggcgaggcg gagaggtgcg gcggcagcca atcagagcgg
481 cgcgctccga aagtttcctt ttatggcgag gcggcggcgg cggcggccct ataaaaagcg
541 aagcgcgcgg cgggcgggag tcgctgcgcg ctgccttcgc cccgtgcccc gctccgccgc
601 cgcctcgcgc cgcccgcccc ggctctgact gaccgcgtta ctcccacagg tgagcgggcg
661 ggacggccct tctcctccgg gctgtaatta gcgcttggtt taatgacggc ttgtttcttt
721 tctgtggctg cgtgaaagcc ttgaggggct ccgggagggc cctttgtgcg gggggagcgg
781 ctcggggctg tccgcggggg gacggctgcc ttcggggggg acggggcagg gcggggttcg
841 gcttctggcg tgtgaccggc ggctctagag cctctgctaa ccatgttcat gccttcttct
901 ttttcctaca gctcctgggc aacgtgctgg ttattgtgct gtctcatcat tttggcaaag
961 aattggatcc ggtaccgcca ccatggccgg cctgagacac accttcgtgg tggccgacgc
1021 caccctgccc gactgccccc tggtgtacgc cagcgagggc ttctacgcca tgaccggcta
1081 cggccccgac gaggtgctgg gccacaacgc cagattcctg cagggcgagg gcaccgaccc
1141 caaggaggtg cagaagatca gagacgccat caagaagggc gaggcctgca gcgtgagact
1201 gctgaactac agaaaggacg gcaccccctt ctggaacctg ctgaccgtga cccccatcaa
1261 gacccccgac ggcagagtga gcaagttcgt gggcgtgcag gtggacgtga ccagcaagac
1321 cgagggcaag gccctggccc gaccggttca cgtgggaagt ggggctacaa acttctcact
1381 gctgaaacag gccggggatg tggaggaaaa cccaggacca cacgtgacta gtatggtgag
1441 cgagctgatt aaggagaaca tgcccatgaa gctgtacatg gagggcaccg tgaacaacca
1501 ccacttcaag tgcacatccg agggcgaagg caagccctac gagggcaccc agaccatgag
1561 aatcaaggtc gtcgagggcg gccctctccc cttcgccttc gacatcctgg ctaccagctt
1621 catgtacggc agcagaacct tcatcaagca ccctccgggc atccccgact tctttaagca
1681 gtccttccct gagggcttca catgggagag agtcaccaca tacgaagacg ggggcgtgct
1741 gaccgctacc caggacacca gcctccagga cggctgcctc atctacaacg tcaaggttag
1801 aggggtgaac ttcccagcca acggccctgt gatgcagaag aaaacactcg gctgggaggc
1861 ctccaccgag acgatgtacc ctgctgacgg aggactggaa ggcgcatgtg acatggctct
1921 gaagctcgtg ggaggaggac acctgatctg caaccttgag acaacataca gatccaagaa
1981 acccgctacg aacctcaaga tgcccggcgt ctacaacgtg gaccacagac tggaaagaat
2041 caaggaggcc gacgatgaga catacgtcga gcagcacgag gtggctgtgg ccagatactc
2101 tactggtggc gctggtgatg gaggtaaata agaattcgat atcaagctta tcgataatca
2161 acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg ttgctccttt
2221 tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt cccgtatggc
2281 tttcattttc tcctccttgt ataaatcctg gttgctgtct ctttatgagg agttgtggcc
2341 cgttgtcagg caacgtggcg tggtgtgcac tgtgtttgct gacgcaaccc ccactggttg
2401 gggcattgcc accacctgtc agctcctttc cgggactttc gctttccccc tccctattgc
2461 cacggcggaa ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg
2521 cactgacaat tccgtggtgt tgtcggggaa atcatcgtcc tttccttggc tgctcgcctg
2581 tgttgccacc tggattctgc gcgggacgtc cttctgctac gtcccttcgg ccctcaatcc
2641 agcggacctt ccttcccgcg gcctgctgcc ggctctgcgg cctcttccgc gtcttcgcct
2701 tcgccctcag acgagtcgga tctccctttg ggccgcctcc ccgcatcgat accgtcgacc
2761 cgggcggccg cttcgagcag acatgataag atacattgat gagtttggac aaaccacaac
2821 tagaatgcag tgaaaaaaat gctttatttg tgaaatttgt gatgctattg ctttatttgt
2881 aaccattata agctgcaata aacaagttaa caacaacaat tgcattcatt ttatgtttca
2941 ggttcagggg gagatgtggg aggtttttta aagcaagtaa aacctctaca aatgtggtaa
3001 aatcgataag gatcttccta gagcatggct acgtagataa gtagcatggc gggttaatca
3061 ttaactacac tgcattaatg aatcggccaa cgcgcgggga gaggcggttt gcgtattggg
3121 cgctcttccg cttcctcgct cactgactcg ctgcgctcgg tcgttcggct gcggcgagcg
3181 gtatcagctc actcaaaggc ggtaatacgg ttatccacag aatcagggga taacgcagga
3241 aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc gtaaaaaggc cgcgttgctg
3301 gcgtttttcc ataggctccg cccccctgac gagcatcaca aaaatcgacg ctcaagtcag
3361 aggtggcgaa acccgacagg actataaaga taccaggcgt ttccccctgg aagctccctc
3421 gtgcgctctc ctgttccgac cctgccgctt accggatacc tgtccgcctt tctcccttcg
3481 ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc tcagttcggt gtaggtcgtt
3541 cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc ccgaccgctg cgccttatcc
3601 ggtaactatc gtcttgagtc caacccggta agacacgact tatcgccact ggcagcagcc
3661 actggtaaca ggattagcag agcgaggtat gtaggcggtg ctacagagtt cttgaagtgg
3721 tggcctaact acggctacac tagaagaaca gtatttggta tctgcgctct gctgaagcca
3781 gttaccttcg gaaaaagagt tggtagctct tgatccggca aacaaaccac cgctggtagc
3841 ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa aaaaaggatc tcaagaagat
3901 cctttgatct tttctacggg gtctgacgct cagtggaacg aaaactcacg ttaagggatt
3961 ttggtcatga gattatcaaa aaggatcttc acctagatcc ttttaaatta aaaatgaagt
4021 tttaaatcaa tctaaagtat atatgagtaa acttggtctg acagttacca atgcttaatc
4081 agtgaggcac ctatctcagc gatctgtcta tttcgttcat ccatagttgc ctgactcccc
4141 gtcgtgtaga taactacgat acgggagggc ttaccatctg gccccagtgc tgcaatgata
4201 ccgcgagacc cacgctcacc ggctccagat ttatcagcaa taaaccagcc agccggaagg
4261 gccgagcgca gaagtggtcc tgcaacttta tccgcctcca tccagtctat taattgttgc
4321 cgggaagcta gagtaagtag ttcgccagtt aatagtttgc gcaacgttgt tgccattgct
4381 acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt cattcagctc cggttcccaa
4441 cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa aagcggttag ctccttcggt
4501 cctccgatcg ttgtcagaag taagttggcc gcagtgttat cactcatggt tatggcagca
4561 ctgcataatt ctcttactgt catgccatcc gtaagatgct tttctgtgac tggtgagtac
4621 tcaaccaagt cattctgaga atagtgtatg cggcgaccga gttgctcttg cccggcgtca
4681 atacgggata ataccgcgcc acatagcaga actttaaaag tgctcatcat tggaaaacgt
4741 tcttcggggc gaaaactctc aaggatctta ccgctgttga gatccagttc gatgtaaccc
4801 actcgtgcac ccaactgatc ttcagcatct tttactttca ccagcgtttc tgggtgagca
4861 aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg cgacacggaa atgttgaata
4921 ctcatactct tcctttttca atattattga agcatttatc agggttattg tctcatgagc
4981 ggatacatat ttgaatgtat ttagaaaaat aaacaaatag gggttccgcg cacatttccc
5041 cgaaaagtgc cacctaaatt gtaagcgtta atattttgtt aaaattcgcg ttaaattttt
5101 gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct tataaatcaa
5161 aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt ccactattaa
5221 agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat ggcccactac
5281 gtgaaccatc accctaatca agttttttgg ggtcgaggtg ccgtaaagca ctaaatcgga
5341 accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac gtggcgagaa
5401 aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta gcggtcacgc
5461 tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg tcccattcgc
5521 cattcaggct gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc
5581 agctgcgcgc tcgctcgctc actgaggccg cccgggcaaa gcccgggcgt cgggcgacct
5641 ttggtcgccc ggcctcagtg agcgagcgag cgcgcagaga gggagtggcc aactccatca
5701 ctaggggttc cttgtagtta atgattaacc cgccatgcta ct
//
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