pAAVLINKv1-pCALM1-Cre-SRCAP-C-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-SRCAP-C-FLAG             7169 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7169)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 17, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7169
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3873
                     /codon_start=2
                     /gene="SRCAP"
                     /gene_synonym="DEHMBA; DOMO1; EAF1; FLHS; SWR1"
                     /product="helicase SRCAP"
                     /label=SRCAP
                     /note="Snf2-related CBP activator protein;Swi2/Snf2-related
                     ATPase homolog, domino homolog 1; domino homolog 2"
                     /db_xref="GI:146219843"
                     /db_xref="CCDS:CCDS10689.2"
                     /db_xref="GeneID:10847"
                     /db_xref="HGNC:HGNC:16974"
                     /db_xref="MIM:611421"
                     /protein_id="NP_006653.2"
                     /translation="ERVPRPAPRPRPTPASAPAAIPALVPVPVSAPVPISAPNPITILP
                     VHILPSPPPPSQIPPCSSPACTPPPACTPPPAHTPPPAQTCLVTPSSPLLLGPPSVPIS
                     ASVTNLPLGLRPEAELCAQALASPESLELASVASSETSSLSLVPPKDLLPVAVEILPVS
                     EKNLSLTPSAPSLTLEAGSIPNGQEQEAPDSAEGTTLTVLPEGEELPLCVSESNGLELP
                     PSAASDEPLQEPLEADRTSEELTEAKTPTSSPEKPQELVTAEVAAPSTSSSATSSPEGP
                     SPARPPRRRTSADVEIRGQGTGRPGQPPGPKVLRKLPGRLVTVVEEKELVRRRRQQRGA
                     ASTLVPGVSETSASPGSPSVRSMSGPESSPPIGGPCEAAPSSSLPTPPQQPFIARRHIE
                     LGVTGGGSPENGDGALLAITPPAVKRRRGRPPKKNRSPADAGRGVDEAPSSTLKGKTNG
                     ADPVPGPETLIVADPVLEPQLIPGPQPLGPQPVHRPNPLLSPVEKRRRGRPPKARDLPI
                     PGTISSAGDGNSESRTQPPPHPSPLTPLPPLLVCPTATVANTVTTVTISTSPPKRKRGR
                     PPKNPPSPRPSQLPVLDRDSTSVLESCGLGRRRQPQGQGESEGSSSDEDGSRPLTRLAR
                     LRLEAEGMRGRKSGGSMVVAVIQDDLDLADSGPGGLELTPPVVSLTPKLRSTRLRPGSL
                     VPPLETEKLPRKRAGAPVGGSPGLAKRGRLQPPSPLGPEGSVEESEAEASGEEEEGDGT
                     PRRRPGPRRLVGTTNQGDQRILRSSAPPSLAGPAVSHRGRKAKT"
     misc_feature    1642..1647
                     /label=SDS
     misc_feature    1657..1662
                     /label=SDS
     misc_feature    1684..1689
                     /label=SDS
     misc_feature    1687..1692
                     /label=SDS
     misc_feature    1904..1909
                     /label=SDS
     misc_feature    2048..2053
                     /label=SDS
     CDS             complement(2137..2148)
                     /codon_start=1
                     /product="WELQut protease recognition and cleavage site"
                     /label=WELQut site
                     /translation="WELQ"
     misc_feature    2338..2343
                     /label=SDS
     misc_feature    2970..2975
                     /label=SDS
     misc_feature    3719..3724
                     /label=SDS
     misc_feature    3722..3727
                     /label=SDS
     CDS             3877..3942
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3958..4389
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4102..4107
                     /label=SDS
     repeat_region   4432..4572
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4432..4561
                     /label=AAV2 ITR
     repeat_region   4433..4573
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4647..5102
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5384..5488
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5489..6349
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6520..7108
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggagcgagt tcccaggcca
     1501 gcacctaggc ctcgacccac tccagcttca gctccggctg caattcctgc ccttgttcct
     1561 gtcccagttt ctgccccagt acccatttca gccccaaatc caataaccat tctccctgtc
     1621 catatcttgc cttctcctcc ccctccttca cagattcctc cttgttcttc tcctgcctgc
     1681 acccctcctc ctgcctgtac ccctccacca gctcatacac cgcctccagc ccaaacctgt
     1741 cttgtaactc cttcctctcc tctcttgctt ggtccacctt ctgtgcccat ctctgcctca
     1801 gtcactaatc tccccttggg cttgaggcct gaggcagagc tgtgtgccca ggcattggca
     1861 tctccagagt ccctggagct ggcttctgtg gccagttcag aaacctcctc actttctctt
     1921 gtgcccccta aagatctgtt gccagttgct gtggagatcc tgcctgtgtc agagaagaac
     1981 ctttctctca ccccttctgc acccagcctg accttggagg ctggcagcat ccccaatggt
     2041 caagagcagg aggcaccaga ttctgctgag gggaccaccc ttacagtgct gcctgaaggt
     2101 gaggagttgc ccctgtgtgt gagtgagagc aatggcctgg agctcccacc ctcagcagca
     2161 tctgatgagc cacttcagga gccactggag gctgacagga cctcggaaga gctgacagag
     2221 gccaagaccc caacctccag cccagagaag ccacaggaac tcgttacagc tgaggttgca
     2281 gctccatcca cctcatcttc agccacttcc tcgcctgagg gtccttcacc tgcccgacct
     2341 cctcggcgtc gcaccagtgc tgatgtggaa attaggggtc aagggactgg tcggccagga
     2401 caaccaccag gccccaaagt gcttcgaaag ctgccaggac ggctggtaac tgtggtagag
     2461 gaaaaggaac tggtgcggcg gcggcggcag cagcggggag ctgccagcac cctagtgcct
     2521 ggggtctctg agactagtgc cagcccggga agcccgtctg tccgcagcat gtcagggcca
     2581 gaatcctccc ctcccattgg tgggccctgt gaagctgctc cttcatcctc actgcccact
     2641 ccaccccagc agcccttcat tgctcgccgt cacattgagc tgggggtgac tggtggtggc
     2701 agccccgaga atggagacgg agcactgctc gccatcaccc cacctgctgt gaaacgtcgg
     2761 agggggaggc cccccaagaa gaacaggtct ccagcagatg ctgggagagg tgtggatgag
     2821 gcaccctcat ccaccttgaa gggaaaaacc aatggggctg acccagtccc tgggcctgag
     2881 accctaattg ttgcagatcc tgtcctggaa ccacagctta ttcctgggcc ccagcctctt
     2941 ggaccccagc cagttcacag acccaatccc ctcctgtcac ctgtggagaa aagaaggcga
     3001 ggacgacccc ctaaagcacg agatttgccc atccctggga ccatttcctc tgcaggggat
     3061 ggcaactccg aaagtcggac acagccaccc ccacacccat cacccctaac cccactccca
     3121 ccactgctag tttgtcccac tgctactgtt gccaacactg tcaccactgt caccatttca
     3181 acgtccccac ccaaacggaa gaggggccga cctcccaaga atcctccatc acctcggccc
     3241 agccagctcc ccgtcttgga ccgtgacagc acttctgttc tcgagagctg tggattgggg
     3301 aggcgacggc aaccccaggg ccaaggggag agtgagggta gttcctctga tgaggatgga
     3361 agccgccccc tcacccgcct ggcccgcctt cggcttgaag cagaaggaat gcgaggacgg
     3421 aagagtggag ggtccatggt ggtggctgta attcaggatg acctggactt agcagatagc
     3481 gggccaggcg ggttggaatt gacaccacct gtggtctcac taaccccaaa actgcgctcg
     3541 acccggctgc gtccagggtc tctagtcccc ccactagaga ctgagaagtt gcctcgcaaa
     3601 cgagcagggg ccccagttgg tgggagtcct gggctggcaa agcggggccg cctacagccc
     3661 ccaagtcccc tggggcctga gggttcagta gaggagtctg aggctgaagc ctcaggtgag
     3721 gaggaggaag gggatgggac cccacgccga cgtcctggcc cccgccggct tgttgggacc
     3781 accaaccaag gggaccagcg catcctgcgc agcagcgccc ctccctccct ggctggccct
     3841 gctgttagtc acagaggccg caaggccaag acgaaggact acaaagacca tgacggtgat
     3901 tataaagatc atgacatcga ctacaaggat gacgatgaca agtaagaatt ctgatgactc
     3961 gagataatca acctctggat tacaaaattt gtgaaagatt gactggtatt cttaactatg
     4021 ttgctccttt tacgctatgt ggatacgctg ctttaatgcc tttgtatcat gctattgctt
     4081 cccgtatggc tttcattttc tcctccttgt ataaatcctg gttagttctt gccacggcgg
     4141 aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg ggcactgaca
     4201 attccgtggt gtttatttgt gaaatttgtg atgctattgc tttatttgta accatctagc
     4261 tttatttgtg aaatttgtga tgctattgct ttatttgtaa ccattataag ctgcaataaa
     4321 caagttaaca acaacaattg cattcatttt atgtttcagg ttcaggggga gatgtgggag
     4381 gttttttaaa gatctggaga ccggtgtggc acgtgcggac cgagcggccg caggaacccc
     4441 tagtgatgga gttggccact ccctctctgc gcgctcgctc gctcactgag gccgggcgac
     4501 caaaggtcgc ccgacgcccg ggctttgccc gggcggcctc agtgagcgag cgagcgcgca
     4561 gctgcctgca ggggcgcctg atgcggtatt ttctccttac gcatctgtgc ggtatttcac
     4621 accgcatacg tcaaagcaac catagtacgc gccctgtagc ggcgcattaa gcgcggcggg
     4681 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccttagcgc ccgctccttt
     4741 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
     4801 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
     4861 tttgggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
     4921 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaactc
     4981 tatctcgggc tattcttttg atttataagg gattttgccg atttcggtct attggttaaa
     5041 aaatgagctg atttaacaaa aatttaacgc gaattttaac aaaatattaa cgtttacaat
     5101 tttatggtgc actctcagta caatctgctc tgatgccgca tagttaagcc agccccgaca
     5161 cccgccaaca cccgctgacg cgccctgacg ggcttgtctg ctcccggcat ccgcttacag
     5221 acaagctgtg accgtctccg ggagctgcat gtgtcagagg ttttcaccgt catcaccgaa
     5281 acgcgcgaga cgaaagggcc tcgtgatacg cctattttta taggttaatg tcatgataat
     5341 aatggtttct tagacgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     5401 tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     5461 gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg tcgcccttat
     5521 tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc tggtgaaagt
     5581 aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg atctcaacag
     5641 cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga gcacttttaa
     5701 agttctgcta tgtggcgcgg tattatcccg tattgacgcc gggcaagagc aactcggtcg
     5761 ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag aaaagcatct
     5821 tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga gtgataacac
     5881 tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg cttttttgca
     5941 caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga atgaagccat
     6001 accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt tgcgcaaact
     6061 attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact ggatggaggc
     6121 ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt ttattgctga
     6181 taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg ggccagatgg
     6241 taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta tggatgaacg
     6301 aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac tgtcagacca
     6361 agtttactca tatatacttt agattgattt aaaacttcat ttttaattta aaaggatcta
     6421 ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt tttcgttcca
     6481 ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt tttttctgcg
     6541 cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt gtttgccgga
     6601 tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc agataccaaa
     6661 tactgttctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc
     6721 tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg
     6781 tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac
     6841 ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct
     6901 acagcgtgag ctatgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc
     6961 ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg
     7021 gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg
     7081 ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcggcctttt tacggttcct
     7141 ggccttttgc tggccttttg ctcacatgt
//