pRosa26-EF1a-hCas9IRESneo

LOCUS       pRosa26-EF1a-hCas9IRESneo               19396 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  Mouse Rosa26 targeting vector carrying the EF1a-hCas9-IRES-neo 
            cassette.
ACCESSION   .
VERSION     .
KEYWORDS    pRosa26-EF1a-hCas9IRESneo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 19396)
  AUTHORS   Tzelepis K, Koike-Yusa H, De Braekeleer E, Li Y, Metzakopian E, 
            Dovey OM, Mupo A, Grinkevich V, Li M, Mazan M, Gozdecka M, Ohnishi 
            S, Cooper J, Patel M, McKerrell T, Chen B, Domingues AF, Gallipoli 
            P, Teichmann S, Ponstingl H, McDermott U, Saez-Rodriguez J, Huntly 
            BJ, Iorio F, Pina C, Vassiliou GS, Yusa K
  TITLE     A CRISPR Dropout Screen Identifies Genetic Vulnerabilities and 
            Therapeutic Targets in Acute Myeloid Leukemia.
  JOURNAL   Cell Rep. 2016 Oct 18;17(4):1193-1205. doi: 
            10.1016/j.celrep.2016.09.079.
  PUBMED    27760321
REFERENCE   2  (bases 1 to 19396)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..19396
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     rep_origin      complement(3..458)
                     /direction=LEFT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     primer_bind     complement(140..161)
                     /label=F1ori-F
                     /note="F1 origin, forward primer"
     primer_bind     352..371
                     /label=F1ori-R
                     /note="F1 origin, reverse primer"
     primer_bind     585..607
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     599..616
                     /label=M13 Forward
                     /note="In lacZ gene. Also called M13-F20 or M13 (-21) 
                     Forward"
     primer_bind     600..616
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     626..645
                     /label=T7
                     /note="T7 promoter, forward primer"
     promoter        626..644
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
     misc_feature    2969..4051
                     /label=Rosa26 left arm
                     /note="left homology arm from intron 1 of the mouse Rosa26 
                     gene (Soriano, 1999)"
     misc_feature    4058..4161
                     /label=Ad SA
                     /note="adenovirus major late transcript splice acceptor 
                     (Friedrich and Soriano, 1991)"
     protein_bind    4181..4214
                     /label=loxP
                     /bound_moiety="Cre recombinase"
                     /note="Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     protein_bind    4215..4239
                     /gene="mutant version of attB"
                     /label=attB1
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     promoter        4691..5871
                     /label=EF-1-alpha promoter
                     /note="strong constitutive promoter for human elongation 
                     factor EF-1-alpha"
     intron          4921..5863
                     /label=EF-1-alpha intron A
                     /note="intron upstream of the start codon of human 
                     EF-1-alpha"
     primer_bind     5820..5840
                     /label=EF1a-F
                     /note="Human elongation factor-1a promoter, forward primer"
     CDS             5890..9993
                     /codon_start=1
                     /product="Cas9 (Csn1) endonuclease from the Streptococcus 
                     pyogenes Type II CRISPR/Cas system"
                     /label=Cas9
                     /note="generates RNA-guided double strand breaks in DNA"
                     /translation="MDKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKK
                     NLIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEES
                     FLVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIK
                     FRGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRL
                     ENLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQ
                     IGDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVR
                     QQLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLL
                     RKQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARG
                     NSRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEY
                     FTVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFD
                     SVEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEER
                     LKTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFM
                     QLIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGR
                     HKPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLY
                     LYYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVP
                     SEEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKH
                     VAQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYL
                     NAVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKT
                     EITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSK
                     ESILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGI
                     TIMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNE
                     LALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILAD
                     ANLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVL
                     DATLIHQSITGLYETRIDLSQLGGD"
     CDS             10006..10026
                     /codon_start=1
                     /product="nuclear localization signal of SV40 (simian virus
                     40) large T antigen"
                     /label=SV40 NLS
                     /translation="PKKKRKV"
     misc_feature    10076..10662
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(10243..10260)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     10470..10489
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             10663..11466
                     /codon_start=1
                     /gene="aph(3')-II (or nptII)"
                     /product="aminoglycoside phosphotransferase from Tn5"
                     /label=NeoR/KanR
                     /note="confers resistance to neomycin, kanamycin, and G418 
                     (Geneticin(R))"
                     /translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
                     GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
                     LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
                     EHQGLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
                     DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
     primer_bind     complement(10726..10745)
                     /label=Neo-R
                     /note="Neomycin resistance gene, reverse primer"
     primer_bind     11336..11355
                     /label=Neo-F
                     /note="Neomycin resistance gene, forward primer"
     primer_bind     complement(11498..11515)
                     /label=BGH-rev
                     /note="Bovine growth hormone terminator, reverse primer. 
                     Also called BGH reverse"
     polyA_signal    11504..11728
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     protein_bind    complement(11773..11797)
                     /gene="mutant version of attB"
                     /label=attB2
                     /bound_moiety="BP Clonase(TM)"
                     /note="recombination site for the Gateway(R) BP reaction"
     polyA_signal    11807..12031
                     /label=bGH poly(A) signal
                     /note="bovine growth hormone polyadenylation signal"
     misc_feature    12044..12854
                     /label=Rosa26 right arm
                     /note="right homology arm from intron 1 of the mouse Rosa26
                     gene (Soriano, 1999)"
     CDS             15928..16500
                     /codon_start=1
                     /gene="Corynebacterium diphtheriae tox"
                     /product="diphtheria toxin fragment A"
                     /label=DTA
                     /translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
                     DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
                     GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
                     NFETRGKRGQDAMYEYMAQACAGNRVRR"
     intron          16577..16642
                     /label=small t intron
                     /note="SV40 (simian virus 40) small t antigen intron"
     primer_bind     complement(17208..17228)
                     /label=T3
                     /note="T3 promoter, forward primer"
     promoter        complement(17208..17226)
                     /label=T3 promoter
                     /note="promoter for bacteriophage T3 RNA polymerase"
     primer_bind     complement(17247..17263)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     primer_bind     complement(17247..17263)
                     /label=M13 Reverse
                     /note="In lacZ gene. Also called M13-rev"
     primer_bind     complement(17260..17282)
                     /label=M13/pUC Reverse
                     /note="In lacZ gene"
     protein_bind    17271..17287
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(17295..17325)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    17340..17361
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(17478..17495)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(17649..18237)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(17729..17748)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(18408..19268)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     19031..19050
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(19269..19373)
                     /gene="bla"
                     /label=AmpR promoter
ORIGIN
        1 ctaaattgta agcgttaata ttttgttaaa attcgcgtta aatttttgtt aaatcagctc
       61 attttttaac caataggccg aaatcggcaa aatcccttat aaatcaaaag aatagaccga
      121 gatagggttg agtgttgttc cagtttggaa caagagtcca ctattaaaga acgtggactc
      181 caacgtcaaa gggcgaaaaa ccgtctatca gggcgatggc ccactacgtg aaccatcacc
      241 ctaatcaagt tttttggggt cgaggtgccg taaagcacta aatcggaacc ctaaagggag
      301 cccccgattt agagcttgac ggggaaagcc ggcgaacgtg gcgagaaagg aagggaagaa
      361 agcgaaagga gcgggcgcta gggcgctggc aagtgtagcg gtcacgctgc gcgtaaccac
      421 cacacccgcc gcgcttaatg cgccgctaca gggcgcgtcc cattcgccat tcaggctgcg
      481 caactgttgg gaagggcgat cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg
      541 gggatgtgct gcaaggcgat taagttgggt aacgccaggg ttttcccagt cacgacgttg
      601 taaaacgacg gccagtgagc gcgcgtaata cgactcacta tagggcgaat tggagctcgt
      661 ttaaacggcg cgccggtacc ttaattaaac cagcaatgtg gatataagca ttaagtttta
      721 gttttaaaaa ggtcaatttt ccaaacattc agcaatcata tttaaattta cagctaggaa
      781 caagagcctt gggtcatgtc ctaccaaaga acataactca atattctaca catgacaatc
      841 tgaataacct taaagcctct aatcccataa caggccacaa attttggaca gagaactaat
      901 gatcctcctg agaaaactgg aagaaatcca gggaaaagaa attcctgtgt cctccaaact
      961 cagaaatctc taattatgtc agtattctct gctttagtcc taggtcagat tgcacacatc
     1021 taaaataacc tcttaaagtt ttcctcctag cgacctaaac cattattaat atcaaattaa
     1081 ccatcaaaac actttcctct caatatgctg cacacaaacc tcctcctgga acctcctcca
     1141 tctggatcct ccccaatcaa aagtataggt atttaacata taagcaagga agtaatgtaa
     1201 acatgacctt ggtcacaaat atgtcatcta aaaacaattt agtcaaggta tggaggaaat
     1261 tcgagaacct gaatcttttt aagtattttg agcacaggaa caattggcaa aaggaatcca
     1321 ggtatagaca aaacccagag cccagagctc tgggcgaaaa atgagttgct ggtgaagacg
     1381 ttacacaagt aacatgagaa agcagaaaat gcaggtcatc cacgcacccc tgacccaggc
     1441 cagcagggcg ggctgcagca tcagtacaca ggagaaagat ccttattcct aagaatgaga
     1501 aaggcaaagg cgcccgatag aataaattag catagaaggg gctttcccag gagttaaaac
     1561 tttccttctg agcgattacc tactaaaacc agggcttttg cccactacca tttacctagg
     1621 atcttggctt gcacggattc ataggggcat atccctcccc ctcttcttta gagtcgttct
     1681 taaaagatcg ctctccacgc cctaggcagg gaaaacgaca aaatctggct caattccagg
     1741 ctagaaccct acaaattcaa cagggatatc gcaaggatac tggggcatac gccacaggga
     1801 gtccaagaat gtgaggtggg ggtggcgaag gtaatgtctt tggtgtggga aaagcagcag
     1861 ccatctgaga taggaactgg aaaaccagag gagaggcgtt caggaagatt atggagggga
     1921 ggactgggcc cccacgagcg accagagttg tcacaaggcc gcaagaacag gggaggtggg
     1981 gggctcaggg acagaaaaaa aagtatgtgt attttgagag cagggttggg aggcctctcc
     2041 tgaaaagggt ataaacgtgg agtaggcaat acccaggcaa aaaggggaga ccagagtagg
     2101 gggaggggaa gagtcctgac ccagggaaga cattaaaaag gtagtggggt cgactagatg
     2161 aaggagagcc tttctctctg ggcaagagcg gtgcaatggt gtgtaaaggt agctgagaag
     2221 acgaaaaggg caagcatctt cctgctacca ggctggggag gcccaggccc acgaccccga
     2281 ggagagggaa cgcagggaga ctgaggtgac ccttctttcc cccggggccc ggtcgtgtgg
     2341 ttcggtgtct cttttctgtt ggacccttac cttgacccag gcgctgccgg ggcctgggcc
     2401 cgggctgcgg cgcacggcac tcccgggagg cagcgagact cgagttaggc ccaacgcggc
     2461 gccacggcgt ttcctggccg ggaatggccc gtacccgtga ggtgggggtg gggggcagaa
     2521 aaggcggagc gagcccgagg cggggrgggg gagggccagg ggcggagggg gccggcacta
     2581 ctgtgttggc ggactggcgg gactagggct gcgtgagtct ctgagcgcag gcgggcggcg
     2641 gccgcccctc ccccggcggc ggcagcggcg gcagcggcgg cagctcactc agcccgctgc
     2701 ccgagcggaa acgccactga ccgcacgggg attcccagtg ccggcgccag gggcacgcgg
     2761 gacacgcccc ctcccgccgc gccattggcc tctccgccca ccgccccaca cttattggcc
     2821 ggtgcgccgc caatcagcgg aggctgccgg ggccgcctaa agaagaggct gtgctttggg
     2881 gctccggctc ctcagagagc ctcggctagg taggggatcg ggactctggc gggagggcgg
     2941 cttggtgcgt ttgcggggat gggcggccgc ggcaggccct ccgagcgtgg tggagccgtt
     3001 ctgtgagaca gccgggtacg agtcgtgacg ctggaagggg caagcgggtg gtgggcagga
     3061 atgcggtccg ccctgcagca accggagggg gagggagaag ggagcggaaa agtctccacc
     3121 ggacgcggcc atggctcggg gggggggggg cagcggagga gcgcttccgg ccgacgtctc
     3181 gtcgctgatt ggcttctttt cctcccgccg tgtgtgaaaa cacaaatggc gtgttttggt
     3241 tggcgtaagg cgcctgtcag ttaacggcag ccggagtgcg cagccgccgg cagcctcgct
     3301 ctgcccactg ggtggggcgg gaggtaggtg gggtgaggcg agctggacgt gcgggcgcgg
     3361 tcggcctctg gcggggcggg ggrggggagg gagggtcagc gaaagtagct cgcgcgcgag
     3421 cggccgccca ccctcccctt cctctggggg agtcgtttta cccgccgccg gccgggcctc
     3481 gtcgtctgat tggctctcgg ggcccagaaa actggccctt gccattggct cgtgttcgtg
     3541 caagttgagt ccatccgccg gccagcgggg gcggcgagga ggcgctccca ggttccggcc
     3601 ctcccctcgg ccccgcgccg cagagtctgg ccgcgcgccc ctgcgcaacg tggcaggaag
     3661 cgcgcgctgg gggcggggac gggcagtagg gctgagcggc tgcggggcgg gtgcaagcac
     3721 gtttccgact tgagttgcct caagaggggc gtgctgagcc agacctccat cgcgcactcc
     3781 ggggagtgga gggaaggagc gagggctcag ttgggctgtt ttggaggcag gaagcacttg
     3841 ctctcccaaa gtcgctctga gttgttatca gtaagggagc tgcagtggag taggcgggga
     3901 gaaggccgca cccttctccg gaggggggag gggagtgttg caataccttt ctgggagttc
     3961 tctgctgcct cctggcttct gaggaccgcc ctgggcctgg gagaatccct tccccctctt
     4021 ccctcgtgat ctgcaactcc agtcttacta gtatctgtag ggcgcagtag tccagggttt
     4081 ccttgatgat gtcatactta tcctgtccct tttttttcca cagctcgcgg ttgaggacaa
     4141 actcttcgcg gtctttccag tggggatcga cggtggatcc ataacttcgt atagcataca
     4201 ttatacgaag ttatacaagt ttgtacaaaa aagcaggctg gcgccggaac caattcagtc
     4261 gactggatcc acgcgtgagg ggcgcggaag ccggggcgga ggaggagcca accccgggct
     4321 gggctgagac ccgcagagga agacgctcta gggatttgtc ccggactagc gagatggcaa
     4381 ggctgaggac gggaggctga ttgagaggcg aaggtacacc ctaatctcaa tacaaccttt
     4441 ggagctaagc cagcaatggt agagggaaga ttctgcacgt cccttccagg cggcctcccc
     4501 gtcaccaccc cccccaaccc gccccgaccg gagctgagag taattcatac aaaaggactc
     4561 gcccctgcct tggggaatcc cagggaccgt cgttaaactc ccactaacgt agaacccaga
     4621 gatcgctgcg ttcccgcccc ctcacccgcc cgctctcgtc atcactgagg tggagaagag
     4681 catgcgtgag gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag
     4741 aagttggggg gaggggtcgg caattgaacc ggtgcctaga gaaagtggcg cggggtaaac
     4801 tgggaaagtg atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat
     4861 ataagtgcag tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag
     4921 gtaagtgccg tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg
     4981 ccttgaatta cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt
     5041 tggaagtggg tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg
     5101 agttgaggcc tggcctgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc
     5161 ctgtctcgct gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac
     5221 gctttttttc tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc
     5281 ggtttttggg gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag
     5341 gcggggcctg cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc
     5401 tgctctggtg cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc
     5461 ccggtcggca ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag
     5521 ctcaaaatgg aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa
     5581 aagggccttt ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc
     5641 caggcacctc gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg
     5701 gttttatgcg atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg
     5761 gcacttgatg taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct
     5821 caagcctcag acagtggttc aaagtttttt tcttccattt caggtgtcgt ggaagcttgg
     5881 tcgaccacca tggacaagaa gtactccatt gggctcgata tcggcacaaa cagcgtcggc
     5941 tgggccgtca ttacggacga gtacaaggtg ccgagcaaaa aattcaaagt tctgggcaat
     6001 accgatcgcc acagcataaa gaagaacctc attggcgccc tcctgttcga ctccggggag
     6061 acggccgaag ccacgcggct caaaagaaca gcacggcgca gatatacccg cagaaagaat
     6121 cggatctgct acctgcagga gatctttagt aatgagatgg ctaaggtgga tgactctttc
     6181 ttccataggc tggaggagtc ctttttggtg gaggaggata aaaagcacga gcgccaccca
     6241 atctttggca atatcgtgga cgaggtggcg taccatgaaa agtacccaac catatatcat
     6301 ctgaggaaga agcttgtaga cagtactgat aaggctgact tgcggttgat ctatctcgcg
     6361 ctggcgcata tgatcaaatt tcggggacac ttcctcatcg agggggacct gaacccagac
     6421 aacagcgatg tcgacaaact ctttatccaa ctggttcaga cttacaatca gcttttcgaa
     6481 gagaacccga tcaacgcatc cggagttgac gccaaagcaa tcctgagcgc taggctgtcc
     6541 aaatcccggc ggctcgaaaa cctcatcgca cagctccctg gggagaagaa gaacggcctg
     6601 tttggtaatc ttatcgccct gtcactcggg ctgaccccca actttaaatc taacttcgac
     6661 ctggccgaag atgccaagct tcaactgagc aaagacacct acgatgatga tctcgacaat
     6721 ctgctggccc agatcggcga ccagtacgca gacctttttt tggcggcaaa gaacctgtca
     6781 gacgccattc tgctgagtga tattctgcga gtgaacacgg agatcaccaa agctccgctg
     6841 agcgctagta tgatcaagcg ctatgatgag caccaccaag acttgacttt gctgaaggcc
     6901 cttgtcagac agcaactgcc tgagaagtac aaggaaattt tcttcgatca gtctaaaaat
     6961 ggctacgccg gatacattga cggcggagca agccaggagg aattttacaa atttattaag
     7021 cccatcttgg aaaaaatgga cggcaccgag gagctgctgg taaagcttaa cagagaagat
     7081 ctgttgcgca aacagcgcac tttcgacaat ggaagcatcc cccaccagat tcacctgggc
     7141 gaactgcacg ctatcctcag gcggcaagag gatttctacc cctttttgaa agataacagg
     7201 gaaaagattg agaaaatcct cacatttcgg ataccctact atgtaggccc cctcgcccgg
     7261 ggaaattcca gattcgcgtg gatgactcgc aaatcagaag agaccatcac tccctggaac
     7321 ttcgaggaag tcgtggataa gggggcctct gcccagtcct tcatcgaaag gatgactaac
     7381 tttgataaaa atctgcctaa cgaaaaggtg cttcctaaac actctctgct gtacgagtac
     7441 ttcacagttt ataacgagct caccaaggtc aaatacgtca cagaagggat gagaaagcca
     7501 gcattcctgt ctggagagca gaagaaagct atcgtggacc tcctcttcaa gacgaaccgg
     7561 aaagttaccg tgaaacagct caaagaagac tatttcaaaa agattgaatg tttcgactct
     7621 gttgaaatca gcggagtgga ggatcgcttc aacgcatccc tgggaacgta tcacgatctc
     7681 ctgaaaatca ttaaagacaa ggacttcctg gacaatgagg agaacgagga cattcttgag
     7741 gacattgtcc tcacccttac gttgtttgaa gatagggaga tgattgaaga acgcttgaaa
     7801 acttacgctc atctcttcga cgacaaagtc atgaaacagc tcaagaggcg ccgatataca
     7861 ggatgggggc ggctgtcaag aaaactgatc aatgggatcc gagacaagca gagtggaaag
     7921 acaatcctgg attttcttaa gtccgatgga tttgccaacc ggaacttcat gcagttgatc
     7981 catgatgact ctctcacctt taaggaggac atccagaaag cacaagtttc tggccagggg
     8041 gacagtcttc acgagcacat cgctaatctt gcaggtagcc cagctatcaa aaagggaata
     8101 ctgcagaccg ttaaggtcgt ggatgaactc gtcaaagtaa tgggaaggca taagcccgag
     8161 aatatcgtta tcgagatggc ccgagagaac caaactaccc agaagggaca gaagaacagt
     8221 agggaaagga tgaagaggat tgaagagggt ataaaagaac tggggtccca aatccttaag
     8281 gaacacccag ttgaaaacac ccagcttcag aatgagaagc tctacctgta ctacctgcag
     8341 aacggcaggg acatgtacgt ggatcaggaa ctggacatca atcggctctc cgactacgac
     8401 gtggatcata tcgtgcccca gtcttttctc aaagatgatt ctattgataa taaagtgttg
     8461 acaagatccg ataaaaatag agggaagagt gataacgtcc cctcagaaga agttgtcaag
     8521 aaaatgaaaa attattggcg gcagctgctg aacgccaaac tgatcacaca acggaagttc
     8581 gataatctga ctaaggctga acgaggtggc ctgtctgagt tggataaagc cggcttcatc
     8641 aaaaggcagc ttgttgagac acgccagatc accaagcacg tggcccaaat tctcgattca
     8701 cgcatgaaca ccaagtacga tgaaaatgac aaactgattc gagaggtgaa agttattact
     8761 ctgaagtcta agctggtctc agatttcaga aaggactttc agttttataa ggtgagagag
     8821 atcaacaatt accaccatgc gcatgatgcc tacctgaatg cagtggtagg cactgcactt
     8881 atcaaaaaat atcccaagct tgaatctgaa tttgtttacg gagactataa agtgtacgat
     8941 gttaggaaaa tgatcgcaaa gtctgagcag gaaataggca aggccaccgc taagtacttc
     9001 ttttacagca atattatgaa ttttttcaag accgagatta cactggccaa tggagagatt
     9061 cggaagcgac cacttatcga aacaaacgga gaaacaggag aaatcgtgtg ggacaagggt
     9121 agggatttcg cgacagtccg gaaggtcctg tccatgccgc aggtgaacat cgttaaaaag
     9181 accgaagtac agaccggagg cttctccaag gaaagtatcc tcccgaaaag gaacagcgac
     9241 aagctgatcg cacgcaaaaa agattgggac cccaagaaat acggcggatt cgattctcct
     9301 acagtcgctt acagtgtact ggttgtggcc aaagtggaga aagggaagtc taaaaaactc
     9361 aaaagcgtca aggaactgct gggcatcaca atcatggagc gatcaagctt cgaaaaaaac
     9421 cccatcgact ttctcgaggc gaaaggatat aaagaggtca aaaaagacct catcattaag
     9481 cttcccaagt actctctctt tgagcttgaa aacggccgga aacgaatgct cgctagtgcg
     9541 ggcgagctgc agaaaggtaa cgagctggca ctgccctcta aatacgttaa tttcttgtat
     9601 ctggccagcc actatgaaaa gctcaaaggg tctcccgaag ataatgagca gaagcagctg
     9661 ttcgtggaac aacacaaaca ctaccttgat gagatcatcg agcaaataag cgaattctcc
     9721 aaaagagtga tcctcgccga cgctaacctc gataaggtgc tttctgctta caataagcac
     9781 agggataagc ccatcaggga gcaggcagaa aacattatcc acttgtttac tctgaccaac
     9841 ttgggcgcgc ctgcagcctt caagtacttc gacaccacca tagacagaaa gcggtacacc
     9901 tctacaaagg aggtcctgga cgccacactg attcatcagt caattacggg gctctatgaa
     9961 acaagaatcg acctctctca gctcggtgga gacagcaggg ctgaccccaa gaagaagagg
    10021 aaggtgtgaa agggttcgat ccctaccggt atcgatgtgc ggccgccgcc ccccccccct
    10081 ctccctcccc cccccctaac gttactggcc gaagccgctt ggaataaggc cggtgtgcgt
    10141 ttgtctatat gttattttcc accatattgc cgtcttttgg caatgtgagg gcccggaaac
    10201 ctggccctgt cttcttgacg agcattccta ggggtctttc ccctctcgcc aaaggaatgc
    10261 aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga agcttcttga agacaaacaa
    10321 cgtctgtagc gaccctttgc aggcagcgga accccccacc tggcgacagg tgcctctgcg
    10381 gccaaaagcc acgtgtataa gatacacctg caaaggcggc acaaccccag tgccacgttg
    10441 tgagttggat agttgtggaa agagtcaaat ggctctcctc aagcgtattc aacaaggggc
    10501 tgaaggatgc ccagaaggta ccccattgta tgggatctga tctggggcct cggtgcacat
    10561 gctttacatg tgtttagtcg aggttaaaaa aacgtctagg ccccccgaac cacggggacg
    10621 tggttttcct ttgaaaaaca cgatgataat atggccacaa ccatgggatc ggccattgaa
    10681 caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt cggctatgac
    10741 tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc agcgcagggg
    10801 cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact gcaggacgag
    10861 gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt
    10921 gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca ggatctcctg
    10981 tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat gcggcggctg
    11041 catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg catcgagcga
    11101 gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga agagcatcag
    11161 gggctcgcgc cagccgaact gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat
    11221 ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt
    11281 tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga catagcgttg
    11341 gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt
    11401 tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct tgacgagttc
    11461 ttctgacgta cgcgatgatc tagagctcgc tgatcagcct cgactgtgcc ttctagttgc
    11521 cagccatctg ttgtttgccc ctcccccgtg ccttccttga ccctggaagg tgccactccc
    11581 actgtccttt cctaataaaa tgaggaaatt gcatcgcatt gtctgagtag gtgtcattct
    11641 attctggggg gtggggtggg gcaggacagc aagggggagg attgggaaga caatagcagg
    11701 catgctgggg atgcggtggg ctctatggct tctgaggcgg aaagaacctg caactagtct
    11761 cgagatatct agacccagct ttcttgtaca aagtggtgaa ttccgactgt gccttctagt
    11821 tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga aggtgccact
    11881 cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag taggtgtcat
    11941 tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga agacaatagc
    12001 aggcatgctg gggatgcggt gggctctatg ggtcgacctc gagagatggg cgggagtctt
    12061 ctgggcaggc ttaaaggcta acctggtgtg tgggcgttgt cctgcagggg aattgaacag
    12121 gtgtaaaatt ggagggacaa gacttcccac agattttcgg ttttgtcggg aagtttttta
    12181 ataggggcaa ataaggaaaa tgggaggata ggtagtcatc tggggtttta tgcagcaaaa
    12241 ctacaggtta ttattgcttg tgatccgcct cggagtattt tccatcgagg tagattaaag
    12301 acatgctcac ccgagtttta tactctcctg cttgagatcc ttactacagt atgaaattac
    12361 agtgtcgcga gttagactat gtaagcagaa ttttaatcat ttttaaagag cccagtactt
    12421 catatccatt tctcccgctc cttctgcagc cttatcaaaa ggtattttag aacactcatt
    12481 ttagccccat tttcatttat tatactggct tatccaaccc ctagacagag cattggcatt
    12541 ttccctttcc tgatcttaga agtctgatga ctcatgaaac cagacagatt agttacatac
    12601 accacaaatc gaggctgtag ctggggcctc aacactgcag ttcttttata actccttagt
    12661 acactttttg ttgatccttt gccttgatcc ttaattttca gtgtctatca cctctcccgt
    12721 caggtggtgt tccacatttg ggcctattct cagtccaggg agttttacaa caatagatgt
    12781 attgagaatc caacctaaag cttaactttc cactcccatg aatgcctctc tcctttttct
    12841 ccatttataa actgagctat taaccattaa tggtttccag gtggatgtct cctcccccaa
    12901 tattacctga tgtatcttac atattgccag gctgatattt taagacatta aaaggtatat
    12961 ttcattattg agccacatgg tattgattac tgcttactaa aattttgtca ttgtacacat
    13021 ctgtaaaagg tggttccttt tggaatgcaa agttcaggtg tttgttgtct ttcctgacct
    13081 aaggtcttgt gagcttgtat tttttctatt taagcagtgc tttctcttgg actggcttga
    13141 ctcatggcat tctacacgtt attgctggtc taaatgtgat tttgccaagc ttcttcagga
    13201 cctataattt tgcttgactt gtagccaaac acaagtaaaa tgattaagca acaaatgtat
    13261 ttgtgaagct tggtttttag gttgttgtgt tgtgtgtgct tgtgctctat aataatacta
    13321 tccaggggct ggagaggtgg ctcggagttc aagagcacag actgctcttc cagaagtcct
    13381 gagttcaatt cccagcaacc acatggtggc tcacaaccat ctgtaatggg atctgatgcc
    13441 ctcttctggt gtgtctgaag accacaagtg tattcacatt aaataaataa atcctccttc
    13501 ttcttctttt tttttttttt aaagagaata ctgtctccag tagaatttac tgaagtaatg
    13561 aaatactttg tgtttgttcc aatatggtag ccaataatca aattactctt taagcactgg
    13621 aaatgttacc aaggaactaa tttttatttg aagtgtaact gtggacagag gagccataac
    13681 tgcagacttg tgggatacag aagaccaatg cagactttaa tgtcttttct cttacactaa
    13741 gcaataaaga aataaaaatt gaacttctag tatcctattt gtttaaactg ctagctttac
    13801 ttaacttttg tgcttcatct atacaaagct gaaagctaag tctgcagcca ttactaaaca
    13861 tgaaagcaag taatgataat tttggatttc aaaaatgtag ggccagagtt tagccagcca
    13921 gtggtggtgc ttgcctttat gcctttaatc ccagcactct ggaggcagag acaggcagat
    13981 ctctgagttt gagcccagcc tggtctacac atcaagttct atctaggata gccaggaata
    14041 cacacagaaa ccctgttggg gaggggggct ctgagatttc ataaaattat aattgaagca
    14101 ttccctaatg agccactatg gatgtggcta aatccgtcta cctttctgat gagatttggg
    14161 tattattttt tctgtctctg ctgttggttg ggtcttttga cactgtgggc tttctttaaa
    14221 gcctccttcc tgccatgtgg tctcttgttt gctactaact tcccatggct taaatggcat
    14281 ggctttttgc cttctaaggg cagctgctga gatttgcagc ctgatttcca gggtggggtt
    14341 gggaaatctt tcaaacacta aaattgtcct ttaatttttt ttttaaaaaa tgggttatat
    14401 aataaacctc ataaaatagt tatgaggagt gaggtggact aatattaaat gagtccctcc
    14461 cctataaaag agctattaag gctttttgtc ttatacttaa cttttttttt aaatgtggta
    14521 tctttagaac caagggtctt agagttttag tatacagaaa ctgttgcatc gcttaatcag
    14581 attttctagt ttcaaatcca gagaatccaa attcttcaca gccaaagtca aattaagaat
    14641 ttctgacttt taatgttaat ttgcttactg tgaatataaa aatgatagct tttcctgagg
    14701 cagggtctca ctatgtatct ctgcctgatc tgcaacaaga tatgtagact aaagttctgc
    14761 ctgcttttgt ctcctgaata ctaaggttaa aatgtagtaa tacttttgga acttgcaggt
    14821 cagattcttt tataggggac acactaaggg agcttgggtg atagttggta aaatgtgttt
    14881 caagtgatga aaacttgaat tattatcacc gcaacctact ttttaaaaaa aaaagccagg
    14941 cctgttagag catgcttaag ggatccctag gacttgctga gcacacaaga gtagttactt
    15001 ggcaggctcc tggtgagagc atatttcaaa aaacaaggca gacaaccaag aaactacagt
    15061 taaggttacc tgtctttaaa ccatctgcat atacacaggg atattaaaat attccaaata
    15121 atatttcatt caagttttcc cccatcaaat tgggacatgg atttctccgg tgaataggca
    15181 gagttggaaa ctaaacaaat gttggttttg tgatttgtga aattgttttc aagtgatagt
    15241 taaagcccat gagatacaga acaaagctgc tatttcgagg tctcttggtt tatactcaga
    15301 agcacttctt tgggtttccc tgcactatcc tgatcatgtg ctaggcctac cttaggctga
    15361 ttgttgttca aataaactta agtttcctgt caggtgatgt catatgattt catatatcaa
    15421 ggcaaaacat gttatatatg ttaaacattt gtacttaatg tgaaagttag gtctttgtgg
    15481 gtttgatttt taattttcaa aacctgagct aaataagtca tttttacatg tcttacattt
    15541 ggtggaattg tataattgtg gtttgcaggc aagactctct gacctagtaa ccctacctat
    15601 agagcacttt gctgggacta gtggatcctc tagagtcgag cagtgtggtt ttcaagagga
    15661 agcaaaaagc ctctccaccc aggcctggaa tgtttccacc caatgtcgag cagtgtggtt
    15721 ttgcaagagg aagcaaaaag cctctccacc caggcctgga atgtttccac ccaatgtcga
    15781 gcaaaccccg cccagcgtct tgtcattggc gaattcgaac acgcagatgc agtcggggcg
    15841 gcgcggtccc aggtccactt cgcatattaa ggtgacgcgt gtggcctcga acaccgagcg
    15901 accctgcagg tcctcgccat ggatcctgat gatgttgttg attcttctaa atcttttgtg
    15961 atggaaaact tttcttcgta ccacgggact aaacctggtt atgtagattc cattcaaaaa
    16021 ggtatacaaa agccaaaatc tggtacacaa ggaaattatg acgatgattg gaaagggttt
    16081 tatagtaccg acaataaata cgacgctgcg ggatactctg tagataatga aaacccgctc
    16141 tctggaaaag ctggaggcgt ggtcaaagtg acgtatccag gactgacgaa ggttctcgca
    16201 ctaaaagtgg ataatgccga aactattaag aaagagttag gtttaagtct cactgaaccg
    16261 ttgatggagc aagtcggaac ggaagagttt atcaaaaggt tcggtgatgg tgcttcgcgt
    16321 gtagtgctca gccttccctt cgctgagggg agttctagcg ttgaatatat taataactgg
    16381 gaacaggcga aagcgttaag cgtagaactt gagattaatt ttgaaacccg tggaaaacgt
    16441 ggccaagatg cgatgtatga gtatatggct caagcctgtg caggaaatcg tgtcaggcga
    16501 tctctttgtg aaggaacctt acttctgtgg tgtgacataa ttggacaaac tacctacaga
    16561 gatttaaagc tctaaggtaa atataaaatt tttaagtgta taatgtgtta aactactgat
    16621 tctaattgtt tgtgtatttt agattccaac ctatggaact gatgaatggg agcagtggtg
    16681 gaatgcagat ccactagagt cgagggatgc agaaattgat gatctattaa acaataaaga
    16741 tgtccactaa aatggaagtt tttcctgtca tactttgtta agaagggtga gaacagagta
    16801 cctacatttt gaatggaagg attggagcta cgggggtggg ggtggggtgg gattagataa
    16861 atgcctgctc tttactgaag gctctttact attgctttat gataatgttt catagttgga
    16921 tatcataatt taaacaagca aaaccaaatt aagggccagc tcattcctcc cactcatgat
    16981 ctatagatct atagatctct cgtgggatca ttgtttttct cttgattccc actttgtggt
    17041 tctaagtact gtggtttcca aatgtgtcag tttcatagcc tgaagaacga gatcagcagc
    17101 ctctgttcca catacacttc attctcagta ttgttttgcc aagttctaat tccatcagaa
    17161 gctggtcgat cgactctagc tagatgcggc gtacccagct tttgttccct ttagtgaggg
    17221 ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
    17281 ctcacaattc cacacaacat acgagccggg agcataaagt gtaaagcctg gggtgcctaa
    17341 tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
    17401 ctgtcgtgcc agctgcatta atgaatcggc caacgcgcgg ggagaggcgg tttgcgtatt
    17461 gggcgctctt ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga
    17521 gcggtatcag ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca
    17581 ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg
    17641 ctggcgtttt tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt
    17701 cagaggtggc gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc
    17761 ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct
    17821 tcgggaagcg tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc
    17881 gttcgctcca agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta
    17941 tccggtaact atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca
    18001 gccactggta acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag
    18061 tggtggccta actacggcta cactagaaga acagtatttg gtatctgcgc tctgctgaag
    18121 ccagttacct tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt
    18181 agcggtggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
    18241 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
    18301 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
    18361 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
    18421 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
    18481 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
    18541 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
    18601 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
    18661 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
    18721 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
    18781 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
    18841 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
    18901 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
    18961 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
    19021 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
    19081 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
    19141 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
    19201 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
    19261 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
    19321 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
    19381 ccccgaaaag tgccac
//