| 质粒名称: | pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001729 |
| 产品用途: | 3' AAVLINK plasmid for MAP1A expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | MAP1A |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-MAP1A-C-FLAG 6850 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6850)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6850
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3554
/codon_start=1
/label=MAP1A
/translation="GAREQPEKEAQSPSPPHPIPMGSPTLWPETEAHVSPPLDSHLGPA
RPSLDFPASAFGFSSLQPAPPQLPSPAEPRSAPCGSLAFSGDRALALAPGPPTRTRHDE
YLEVTKAPSLDSSLPQLPSPSSPGAPLLSNLPRPASPALSEGSSSEATTPVISSVAERF
SPSLEAAEQESGELDPGMEPAAHSLWDLTPLSPAPPASLDLALAPAPSLPGDMGDGILP
CHLECSEAATEKPSPFQVPSEDCAANGPTETSPNPPGPAPAKAENEEAAACPAWERGAW
PEGAERSSRPDTLLSPEQPVCPAGGSGGPPSSASPEVEAGPQGCATEPRPHRGELSPSF
LNPPLPPSIDDRDLSTEEVRLVGRGGRRRVGGPGTTGGPCPVTDETPPTSASDSGSSQS
DSDVPPETEECPSITAEAALDSDEDGDFLPVDKAGGVSGTHHPRPGHDPPPLPQPDPRP
SPPRPDVCMADPEGLSSESGRVERLREKEKVQGRVGRRAPGKAKPASPARRLDLRGKRS
PTPGKGPADRASRAPPRPRSTTSQVTPAEEKDGHSPMSKGLVNGLKAGPMALSSKGSSG
APVYVDLAYIPNHCSGKTADLDFFRRVRASYYVVSGNDPANGEPSRAVLDALLEGKAQW
GENLQVTLIPTHDTEVTREWYQQTHEQQQQLNVLVLASSSTVVMQDESFPACKIEF"
misc_feature 1524..1529
/label=SDS
misc_feature 1921..1926
/label=SDS
misc_feature 2826..2831
/label=SDS
CDS 3558..3623
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3639..4070
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3783..3788
/label=SDS
repeat_region 4113..4253
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4113..4242
/label=AAV2 ITR
rep_origin 4328..4783
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5065..5169
/gene="bla"
/label=AmpR promoter
CDS 5170..6030
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6201..6789
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca gggggctcgg gaacagccag
1501 aaaaagaggc ccaatcccca agtcctcctc accccattcc tatggggtcc cccacattat
1561 ggccagaaac tgaggcacat gttagccctc ccttggactc acacctgggg cctgcccgac
1621 ccagtctgga cttccctgct tcagcctttg gcttctcctc attgcagcca gctcccccac
1681 agctgccctc tccagctgaa ccccgctcgg caccctgtgg ctcccttgcc ttctctgggg
1741 atcgagctct ggctctggct ccaggacccc ccaccagaac ccggcatgat gaatacctgg
1801 aagtgaccaa ggcccccagc ctggattcct cactgcccca gctcccatca cccagttctc
1861 ctggggcccc tctcctctcc aatctgccac gacctgcctc accagccctg tctgagggct
1921 cctcctctga ggctaccacg cctgtgattt caagtgtggc ggagcgcttc tctccaagcc
1981 ttgaggctgc agaacaggag tctggagagc tggacccagg aatggaacca gctgcccaca
2041 gcctctggga cctcactcct ctgagcccag cacccccagc ttcactggac ttggccctag
2101 ctccagctcc aagcctgcct ggagacatgg gtgatggcat cctgccgtgc cacctggagt
2161 gctcagaggc agccacggag aagccaagcc ccttccaggt tccctctgag gattgtgcag
2221 ccaatggccc aactgaaacc agccctaacc ccccaggccc tgccccagcc aaggctgaaa
2281 atgaagaggc tgcggcttgc cctgcctggg aacgtggggc ctggcctgaa ggagctgaga
2341 ggagctcccg gcctgacaca ttgctctccc ctgagcagcc agtgtgtcct gcagggggct
2401 ccgggggccc acccagcagt gcctctcctg aggtcgaagc tgggccccag ggatgtgcca
2461 ctgagcctcg gccccatcgt ggggagctct ccccatcctt cctgaaccca cctctgcccc
2521 catccataga tgatagggac ctctcaactg aggaagttcg gctagtagga agaggggggc
2581 ggcgccgggt aggggggcca gggaccactg ggggcccatg ccctgtgact gatgagacac
2641 cccctacatc agccagtgac tcaggctcct cacagtcaga ttctgatgtc ccgccagaaa
2701 ctgaggagtg tccgtccatc acagctgagg cagccctcga ctcagatgaa gatggagact
2761 tcctacctgt ggacaaagct gggggtgtca gtggtactca ccaccccagg cctggccatg
2821 acccacctcc tctcccacag ccagaccccc gcccatcccc tccccgccct gatgtgtgca
2881 tggctgaccc cgaggggctc agctcagagt ctgggagagt agagaggcta cgggagaagg
2941 aaaaggttca ggggcgagta gggcgcaggg ccccaggcaa ggccaagcca gcgtcccctg
3001 cacggcgtct ggatcttcgg ggaaaacgct cacccacccc tggtaaaggg cctgcagatc
3061 gagcatcccg ggccccacct cgaccacgca gcaccacaag ccaggtcacc ccagcagagg
3121 aaaaggatgg acacagcccc atgtccaaag gcctagtcaa tggactcaag gcaggaccaa
3181 tggccttgag ttccaagggc agctctggtg cccctgtata tgtggatctc gcctacatcc
3241 cgaatcattg cagtggcaag actgctgacc ttgacttctt ccgtcgagtg cgtgcatcct
3301 actatgtggt cagtgggaat gaccctgcca atggcgagcc aagccgggct gtgctggatg
3361 ccctgctgga gggcaaggcc cagtgggggg agaatcttca ggtgactctg atccctactc
3421 atgacacgga ggtgactcgt gagtggtacc aacaaactca tgagcagcag caacaactga
3481 atgtcctggt cctggctagc agcagcaccg tggtgatgca ggatgagtcc ttccctgcct
3541 gcaagattga gttcaaggac tacaaagacc atgacggtga ttataaagat catgacatcg
3601 actacaagga tgacgatgac aagtaagaat tctgatgact cgagataatc aacctctgga
3661 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
3721 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
3781 ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg ccgcctgcct
3841 tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg tgtttatttg
3901 tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt gaaatttgtg
3961 atgctattgc tttatttgta accattataa gctgcaataa acaagttaac aacaacaatt
4021 gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa agatctggag
4081 accggtgtgg cacgtgcgga ccgagcggcc gcaggaaccc ctagtgatgg agttggccac
4141 tccctctctg cgcgctcgct cgctcactga ggccgggcga ccaaaggtcg cccgacgccc
4201 gggctttgcc cgggcggcct cagtgagcga gcgagcgcgc agctgcctgc aggggcgcct
4261 gatgcggtat tttctcctta cgcatctgtg cggtatttca caccgcatac gtcaaagcaa
4321 ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
4381 gtgaccgcta cacttgccag cgccttagcg cccgctcctt tcgctttctt cccttccttt
4441 ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
4501 cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga tggttcacgt
4561 agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
4621 aatagtggac tcttgttcca aactggaaca acactcaact ctatctcggg ctattctttt
4681 gatttataag ggattttgcc gatttcggtc tattggttaa aaaatgagct gatttaacaa
4741 aaatttaacg cgaattttaa caaaatatta acgtttacaa ttttatggtg cactctcagt
4801 acaatctgct ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac
4861 gcgccctgac gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc
4921 gggagctgca tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc
4981 ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca
5041 ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat
5101 tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa
5161 aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt
5221 tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag
5281 ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt
5341 tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg
5401 gtattatccc gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag
5461 aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta
5521 agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg
5581 acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta
5641 actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac
5701 accacgatgc ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt
5761 actctagctt cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca
5821 cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag
5881 cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta
5941 gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag
6001 ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt
6061 tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat
6121 aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta
6181 gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa
6241 acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt
6301 tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag
6361 ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta
6421 atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca
6481 agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag
6541 cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa
6601 agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga
6661 acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc
6721 gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc
6781 ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt
6841 gctcacatgt
//
