| 质粒名称: | pYK194 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000798 |
| 产品用途: | Mammalian PhCMV-driven constitutive NLS-NS3a(H1)-AG-FTH1 expression vector (PhCMV-NLS-NS3a(H1)-AG-FTH1-pA). |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | NLS-NS3a(H1)-AG-FTH1 |
| 物种来源: | synthetic |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Programmable solid-state condensates for spatiotemporal control of mammalian gene expression
Wang Y., Jiang J., Xiong Q., Li S., Shao J., Xie M., Zeng A.P.
Nat. Chem. Biol. 2025 1–10 (2025)
|
pYK194
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pYK194载体质粒基本信息
pYK194质粒图谱载体图谱和pYK194载体序列质粒序列多克隆位点信息
pYK194质粒载体简介
pYK194质粒序列载体序列
LOCUS pYK194 7600 bp DNA circular SYN 11-FEB-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7600)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Jul 27, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7600
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
primer_bind 863..882
/label=T7
primer_bind 881..900
/label=oYK06-ANR-mEGFP-F
primer_bind 881..900
/label=oYK36-AG-Link_s-F
primer_bind 881..900
/label=oYK30-NS3a_Head-F
misc_feature 905..964
/label=NLS2
primer_bind 932..985
/label=oYK30-NS3a_Head-F
primer_bind complement(943..987)
/label=oYK163_NLS_R
primer_bind complement(945..967)
/label=oYK54-NLS-R
primer_bind complement(945..967)
/label=oYK57-NLS-R
CDS 968..1555
/codon_start=1
/label=NS3a H1
/translation="KKKGSVVIVGRINLSGDTAYSQQTRGLEGCQETSQTGRDKNQVEG
EVQVVSTATQSFLATSINGVLWTVYHGAGTRTIASPKGPVTQMYTNVDKDLVGWQAPQG
SRSLTPCTCGSSDLYLVTRHADVIPVRGRGDSRGSLLSPRPISYLKGSSGGPLLCPAGH
AVGIFRAAVSTRGVAKAVDFIPVESLETTMRSP"
primer_bind 968..1003
/label=oSCL1082
primer_bind 968..988
/label=oyk03-03-F
primer_bind 968..988
/label=oYK04-ns3a-F
primer_bind 968..986
/label=oYK25-NS3a-F
primer_bind 985..1043
/label=oSCL1083
primer_bind 1024..1082
/label=oSCL1084
primer_bind 1061..1119
/label=oSCL1085
primer_bind 1098..1156
/label=oSCL1086
primer_bind complement(1126..1145)
/label=oSCL1106
primer_bind 1127..1165
/label=oSCL1104
primer_bind complement(1362..1420)
/label=oSCL1105
primer_bind complement(1402..1473)
/label=oSCL1087
primer_bind 1404..1425
/label=oSCL1107
primer_bind 1455..1473
/label=oSCL1088
primer_bind complement(1537..1561)
/label=oSCL1089
primer_bind complement(1540..1572)
/label=oYK30-NS3a_head-R
primer_bind complement(1543..1584)
/label=oYK66-NS3a-R
misc_feature 1562..1572
/label=linker
CDS 1565..1639
/codon_start=1
/label=5xGS Linker
/translation="GSGTGSGTGSGTGTTSGTGTGGSTG"
primer_bind 1565..1585
/label=oYK66-AG-lplA-F
primer_bind 1565..1583
/label=Primer 1
primer_bind complement(1614..1642)
/label=olyl212
primer_bind complement(1614..1639)
/label=olyl234
primer_bind complement(1623..1659)
/label=oYK45-46 ANR1-R
primer_bind 1639..1658
/label=oYK36-AG-Link_s-F
primer_bind 1640..1659
/label=oYK41-44 AG-LplA-F
primer_bind 1640..1659
/label=oYK45-46 AG-lplA-F
misc_feature 1640..1642
/label=Long_Linker
misc_feature 1643..2317
/label=AG
primer_bind 2091..2101
/label=oSCL1104
primer_bind 2298..2334
/label=oYK37-Long_Link-F
primer_bind complement(2302..2317)
/label=oYK37-AG-R
primer_bind complement(2303..2317)
/label=oYK224_AG_R
misc_feature 2318..2545
/label=Long_Linker
primer_bind complement(2525..2545)
/label=oYK81_ANR_AG_R
primer_bind 2526..2562
/label=oYK81_FTH_F
primer_bind complement(2526..2548)
/label=oYK37-Long_Link-R
/note="AGGCGTAATGTGGAgcCCATCATGGATCCTCCTTGGGTCGATC"
gene 2545..3098
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/label=FTH1
/note="ferritin heavy chain 1"
/db_xref="GeneID:2495"
/db_xref="HGNC:HGNC:3976"
/db_xref="MIM:134770"
primer_bind 2545..2562
/label=oCW57
primer_bind 2545..2559
/label=oCW29
CDS 2546..3097
/codon_start=1
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/product="ferritin heavy chain"
/EC_number="1.16.3.1"
/label=FTH1
/note="apoferritin; placenta immunoregulatory
factor;ferritin, heavy polypeptide 1;
proliferation-inducing protein 15; ferritin H subunit; cell
proliferation-inducing gene 15 protein"
/db_xref="CCDS:CCDS41655.1"
/db_xref="GeneID:2495"
/db_xref="HGNC:HGNC:3976"
/db_xref="MIM:134770"
/protein_id="NP_002023.2"
/translation="MTTASTSQVRQNYHQDSEAAINRQINLELYASYVYLSMSYYFDRD
DVALKNFAKYFLHQSHEEREHAEKLMKLQNQRGGRIFLQDIKKPDCDDWESGLNAMECA
LHLEKNVNQSLLELHKLATDKNDPHLCDFIETHYLNEQVKAIKELGDHVTNLRKMGAPE
SGLAEYLFDKHTLGDSDNES"
misc_feature 2546..2548
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="N-acetylmethionine.
/evidence=ECO:0007744|PubMed:22814378; propagated from
UniProtKB/Swiss-Prot (P02794.2); acetylation site"
misc_feature 2549..2551
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="N-acetylthreonine, in Ferritin heavy
chain,N-terminally processed.
/evidence=ECO:0007744|PubMed:22814378; propagated from
UniProtKB/Swiss-Prot (P02794.2); acetylation site"
primer_bind complement(3067..3094)
/label=oYK435_FTH2_R
primer_bind complement(3070..3117)
/label=oYK81_FTH1_R
primer_bind complement(3074..3141)
/label=oCW30
misc_feature 3080..3082
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:17081983,
ECO:0007744|PubMed:18318008, ECO:0007744|PubMed:20068231,
ECO:0007744|PubMed:21406692, ECO:0007744|PubMed:24275569;
propagated from UniProtKB/Swiss-Prot (P02794.2);
phosphorylation site"
misc_feature 3092..3094
/gene="FTH1"
/gene_synonym="FHC; FTH; FTHL6; HFE5; PIG15; PLIF"
/note="Phosphoserine./evidence=ECO:0007744|PubMed:18318008;
propagated from UniProtKB/Swiss-Prot (P02794.2);
phosphorylation site"
primer_bind complement(3095..3117)
/label=oYK224_AG_R
primer_bind complement(3098..3120)
/label=oYK57-MCP-mCherry-R
primer_bind complement(3122..3141)
/label=oSCL1089
terminator 3194..3421
/label=bGH PA
misc_feature 3467..3895
/label=f1 origin
misc_feature 3900..4243
/label=SV40 early promoter and origin
misc_feature 4089..4166
/label=SV40 ORI
CDS 4308..5099
/label=Kan/neoR
misc_feature 5273..5403
/label=SV40 early polyadenylation signal
primer_bind complement(5439..5459)
/label=M13-rev
misc_binding complement(5465..5487)
/label=LacO
promoter complement(5492..5521)
/label=lac
misc_feature complement(5786..6459)
/label=pUC origin
CDS complement(6607..7266)
/label=AmpR
primer_bind 6923..6934
/label=oyk03-03-F
primer_bind 6923..6934
/label=oYK04-ns3a-F
promoter complement(7506..7534)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 caccatgccc aagaagaagc gcaaggtgga agctagcgct tccccgaaga aaaagcggaa
961 agtcatgaag aagaagggca gcgtggtgat cgtgggcaga atcaacctga gcggcgacac
1021 cgcctacagc cagcagacca gaggcctgga gggctgccag gagaccagcc agaccggcag
1081 agacaagaac caggtggagg gcgaggtgca ggtggtgagc accgccaccc agagcttcct
1141 ggccaccagc atcaacggcg tgctgtggac cgtgtaccac ggcgccggca ccagaaccat
1201 cgccagcccc aagggccccg tgacccagat gtacacgaac gtggacaagg acttagtggg
1261 ctggcaggcc ccccaaggca gcagaagcct gaccccctgc acctgcggca gcagcgacct
1321 gtacctggtg accagacacg ccgacgtgat ccccgtgaga ggaagaggcg acagcagagg
1381 cagcctgctg agccccagac ccatcagcta tctgaaaggc tcaagcggcg gccccctgtt
1441 gtgtcccgcc ggccatgccg tgggcatctt cagagccgcc gtgagcacca gaggcgtggc
1501 caaggccgtg gacttcatcc ccgtggagag cctggagacc accatgagaa gccccgctag
1561 cagcggaagt ggaactgggt ctgggactgg gtccggcacc gggactactt caggcaccgg
1621 cactgggggc tccactggca tggtgagcgt gatcaagccc gagatgaaga tcaagctgtg
1681 catgaggggc accgtgaacg gccacaactt cgtgatcgag ggcgagggca agggcaaccc
1741 ctacgagggc acccagatcc tggacctgaa cgtgaccgag ggcgcccccc tgcccttcgc
1801 ctacgacatc ctgaccaccg tgttccagta cggcaacagg gccttcacca agtaccccgc
1861 cgacatccag gactacttca agcagacctt ccccgagggc taccactggg agaggagcat
1921 gacctacgag gaccagggca tctgcaccgc caccagcaac atcagcatga ggggcgactg
1981 cttcttctac gacatcaggt tcgacggcgt gaacttcccc cccaacggcc ccgtgatgca
2041 gaagaagacc ctgaagtggg agcccagcac cgagaagatg tacgtgaggg acggcgtgct
2101 gaagggcgac gtgaacatgg ccctgctgct ggagggcggc ggccactaca ggtgcgactt
2161 caagaccacc tacaaggcca agaaggacgt gaggctgccc gactaccact tcgtggacca
2221 caggatcgag atcctgaagc acgacaagga ctacaacaag gtgaagctgt acgagaacgc
2281 cgtggccagg tacagcatgc tgcccagcca ggccaagacc ggttctggga gtggcggtag
2341 tggtgctgga ggcagcgcag gctctggagc aggcggcagt gccggaagtg gcgctggagg
2401 gtctgccgga tctggagccg gtggttctgc cggctccggc gctggtggga gcgcttccgg
2461 aagtagtccc ggtaattccg ctgacggcgg cggaggatcg ggtggtagtg gtggttcagg
2521 aggaggatcg acccaaggag gatccatgac gaccgcgtcc acctcgcagg tgcgccagaa
2581 ctaccaccag gactcagagg ccgccatcaa ccgccagatc aacctggagc tctacgcctc
2641 ctacgtttac ctgtccatgt cttactactt tgaccgcgat gatgtggctt tgaagaactt
2701 tgccaaatac tttcttcacc aatctcatga ggagagggaa catgctgaga aactgatgaa
2761 gctgcagaac caacgaggtg gccgaatctt ccttcaggat atcaagaaac cagactgtga
2821 tgactgggag agcgggctga atgcaatgga gtgtgcatta catttggaaa aaaatgtgaa
2881 tcagtcacta ctggaactgc acaaactggc cactgacaaa aatgaccccc atttgtgtga
2941 cttcattgag acacattacc tgaatgagca ggtgaaagcc atcaaagaat tgggtgacca
3001 cgtgaccaac ttgcgcaaga tgggagcgcc cgaatctggc ttggcggaat atctctttga
3061 caagcacacc ctgggagaca gtgataatga aagctaagga tccactagtc cagtgtggtg
3121 gaattctgca gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc
3181 cgctgatcag cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc
3241 gtgccttcct tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa
3301 attgcatcgc attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac
3361 agcaaggggg aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg
3421 gcttctgagg cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc
3481 ggcgcattaa gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc
3541 gccctagcgc ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt
3601 ccccgtcaag ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac
3661 ctcgacccca aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag
3721 acggtttttc gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa
3781 actggaacaa cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg
3841 atttcggcct attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc
3901 tgtggaatgt gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta
3961 tgcaaagcat gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag
4021 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa
4081 ctccgcccat cccgccccta actccgccca gttccgccca ttctccgccc catggctgac
4141 taattttttt tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt
4201 agtgaggagg cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat
4261 ccattttcgg atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg
4321 gattgcacgc aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac
4381 aacagacaat cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg
4441 ttctttttgt caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc
4501 ggctatcgtg gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg
4561 aagcgggaag ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc
4621 accttgctcc tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc
4681 ttgatccggc tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta
4741 ctcggatgga agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg
4801 cgccagccga actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg
4861 tgacccatgg cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat
4921 tcatcgactg tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc
4981 gtgatattgc tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta
5041 tcgccgctcc cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag
5101 cgggactctg gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt
5161 cgattccacc gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg
5221 ctggatgatc ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt
5281 tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc
5341 atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt
5401 ctgtataccg tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt
5461 gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
5521 agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
5581 tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
5641 aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
5701 cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
5761 atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
5821 taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
5881 aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
5941 tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
6001 gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
6061 cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
6121 cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
6181 atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
6241 tacagagttc ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat
6301 ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
6361 acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
6421 aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
6481 aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
6541 tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
6601 cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
6661 catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
6721 ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
6781 aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
6841 ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
6901 caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
6961 attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
7021 agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
7081 actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
7141 ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
7201 ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
7261 gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
7321 atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
7381 cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
7441 gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
7501 gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
7561 ggttccgcgc acatttcccc gaaaagtgcc acctgacgtc
//
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