| 质粒名称: | pAAVLINK-3'-CHD8-FLAG-bGHpA |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001700 |
| 产品用途: | 3' AAVLINK plasmid for CHD8 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: |
Promoterless
|
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | CHD8 |
| 物种来源: |
H.sapiens
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-CHD8-FLAG-bGHpA
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-CHD8-FLAG-bGHpA载体质粒基本信息
pAAVLINK-3'-CHD8-FLAG-bGHpA质粒图谱载体图谱和pAAVLINK-3'-CHD8-FLAG-bGHpA载体序列质粒序列多克隆位点信息
pAAVLINK-3'-CHD8-FLAG-bGHpA质粒载体简介
pAAVLINK-3'-CHD8-FLAG-bGHpA质粒序列载体序列
LOCUS pAAVLINK-3'-CHD8-FLAG-bGHpA 7314 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7314)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 3, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7314
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..4254
/codon_start=1
/label=CHD8
/translation="VYRLITRNSYEREMFDKASLKLGLDKAVLQSMSGRDGNITGIQQF
SKKEIEDLLRKGAYAAIMEEDDEGSKFCEEDIDQILLRRTTTITIESEGKGSTFAKASF
VASENRTDISLDDPNFWQKWAKKADLDMDLLNSKNNLVIDTPRVRKQTRHFSTLKDDDL
VEFSDLESEDDERPRSRRHDRHHAYGRTDCFRVEKHLLVYGWGRWRDILSHGRFKRRMT
ERDVETICRAILVYCLLHYRGDENIKGFIWDLISPAENGKTKELQNHSGLSIPVPRGRK
GKKVKSQSTFDIHKADWIRKYNPDTLFQDESYKKHLKHQCNKVLLRVRMLYYLRQEVIG
DQAEKVLGGAIASEIDIWFPVVDQLEVPTTWWDSEADKSLLIGVFKHGYEKYNTMRADP
ALCFLEKAGRPDDKAIAAEHRVLDNFSDIVEGVDFDKDCEDPEYKPLQGPPKDQDDEGD
PLMMMDEEISVIDGDEAQVTQQPGHLFWPPGSALTARLRRLVTAYQRSYKREQMKIEAA
ERGDRRRRRCEAAFKLKEIARREKQQRWTRREQTDFYRVVSTFGVEYDPDTMQFHWDRF
RTFARLDKKTDESLTKYFHGFVAMCRQVCRLPPAAGDEPPDPNLFIEPITEERASRTLY
RIELLRRLREQVLCHPLLEDRLALCQPPGPELPKWWEPVRHDGELLRGAARHGVSQTDC
NIMQDPDFSFLAARMNYMQNHQAGAPAPSLSRCSTPLLHQQYTSRTASPLPLRPDAPVE
KSPEETATQVPSLESLTLKLEHEVVARSRPTPQDYEMRVSPSDTTPLVSRSVPPVKLED
EDDSDSELDLSKLSPSSSSSSSSSSSSSSTDESEDEKEEKLTDQSRSKLYDEESLLSLT
MSQDGFPNEDGEQMTPELLLLQERQRASEWPKDRVLINRIDLVCQAVLSGKWPSSRRSQ
EMVTGGILGPGNHLLDSPSLTPGEYGDSPVPTPRSSSAASMAEEEASAVSTAAAQFTKL
RRGMDEKEFTVQIKDEEGLKLTFQKHKLMANGVMGDGHPLFHKKKGNRKKLVELEVECM
EEPNHLDVDLETRIPVINKVDGTLLVGEDAPRRAELEMWLQGHPEFAVDPRFLAYMEDR
RKQKWQRCKKNNKAELNCLGMEPVQTANSRNGKKGHHTETVFNRVLPGPIAPESSKKRA
RRMRPDLSKMMALMQGGSTGSLSLHNTFQHSSSGLQSVSSLGHSSATSASLPFMPFVMG
GAPSSPHVDSSTMLHHHHHHPHPHHHHHHHPGLRAPGYPSSPVTTASGTTLRLPPLQPE
EDDDEDEEDDDDLSQGYDSSERDFSLIDDPMMPANSDSSEDADD"
misc_feature 1277..1282
/label=SDS
misc_feature 2254..2259
/label=SDS
misc_feature 3072..3077
/label=SDS
misc_feature 3188..3193
/label=SDS
CDS 3988..4005
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 4015..4035
/codon_start=1
/product="6xHis affinity tag"
/label=7xHis
/translation="HHHHHHH"
misc_feature 4121..4126
/label=SDS
CDS 4261..4326
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
polyA_signal 4330..4554
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 4577..4717
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4577..4706
/label=AAV2 ITR
rep_origin 4792..5247
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5529..5633
/gene="bla"
/label=AmpR promoter
CDS 5634..6494
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6665..7253
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtgtaccgcc tcatcactcg taattcctac
301 gagagagaga tgtttgataa ggccagcctc aagttggggt tggataaggc tgtgcttcaa
361 tccatgagtg gtcgggatgg caacattact ggaatccaac agttctctaa gaaggagatt
421 gaagatcttt taagaaaagg agcatatgca gccatcatgg aggaagatga tgaaggctcc
481 aagttttgtg aagaggacat tgaccagatc ttgttaagac gaactacaac catcaccatt
541 gaatctgaag gaaaaggttc cacctttgct aaggcaagct ttgttgcttc tgaaaacagg
601 acagatattt ctttggatga ccccaacttt tggcaaaagt gggccaaaaa ggctgaccta
661 gacatggatc tgctcaacag caagaataat ttggtaattg acacacctag agtacgaaaa
721 caaacgcgcc actttagcac tctgaaagat gatgacctgg tggaattctc tgatttggaa
781 agtgaggatg atgagcggcc acgctcccgc agacatgacc gtcatcatgc ctatgggcgc
841 actgactgct ttcgggtgga aaagcatctc ctggtatatg gttggggacg atggcgagat
901 attttatctc atggacgctt caagcgacgt atgactgaac gagatgtgga gaccatttgt
961 cgggccattc tcgtgtactg tcttctacac taccgtgggg atgaaaatat taaaggcttc
1021 atctgggact tgattagccc agctgaaaat ggcaagacaa aagaattgca gaatcattca
1081 ggtctatcta tccctgtgcc tcgtggacgc aaaggaaaaa aagtaaagtc acaaagcact
1141 tttgatatcc ataaggcaga ttggatccgg aaatataacc ctgacacttt gttccaagat
1201 gaaagttata agaagcactt gaaacatcag tgtaacaagg tactgttgcg ggtacgaatg
1261 ctatactacc tgaggcagga ggttattgga gaccaagcag aaaaggtgtt agggggtgcg
1321 attgccagtg agattgacat atggttccca gtagtggatc aactggaggt tccaacaact
1381 tggtgggaca gtgaggctga caagtcgctg ctcattggag tctttaaaca tggctatgag
1441 aaatataata ccatgagggc agacccagcc ttatgtttcc tagaaaaggc tggccgacca
1501 gatgacaaag caattgcagc agaacatcga gtgttggata acttctctga catagtagaa
1561 ggggttgact ttgataaaga ttgtgaagat cctgaatata aaccactcca aggtccccca
1621 aaggaccaag atgatgaggg tgatcccttg atgatgatgg atgaggagat ctcagtgatt
1681 gatggagatg aagcccaggt gacccaacag ccaggccatt tattctggcc tccgggctct
1741 gccctaacag ctaggcttcg gcgtctagta acagcgtatc agcgcagcta caagagagaa
1801 caaatgaaga tagaggctgc agaacgtggg gaccggcgaa ggcggcgttg tgaagcagcc
1861 ttcaagctga aagaaattgc acggcgggag aaacaacaac gatggacaag gcgtgaacaa
1921 actgattttt atcgagtggt gtctacgttt ggtgtggaat atgaccctga caccatgcag
1981 ttccattggg atcgcttccg cacttttgct cgactagaca aaaagacaga tgaaagcctt
2041 accaagtact tccatggctt tgtggccatg tgccgccaag tatgccgcct tcccccagca
2101 gctggagatg aaccccccga ccctaacctg ttcattgagc ccatcactga ggagagagcc
2161 tcacggactc tctaccgtat agaattgctt cggcgcttac gggaacaagt tttatgccac
2221 ccccttttgg aagatcggct ggcattgtgt cagcctcctg gtcctgaatt gcccaaatgg
2281 tgggagcctg ttcggcatga tggggagctt ctaagagggg cagcccgcca tggggtgagc
2341 caaacagact gcaacatcat gcaggaccca gacttctctt ttctggctgc ccgtatgaat
2401 tatatgcaga accatcaagc aggagcacca gctccatcct tgtcacgctg ctctactcca
2461 ctgctgcacc agcagtatac ctcacgcact gcctcaccac tgcccctgcg cccagatgct
2521 cctgttgaaa agtcacccga ggagacagct acccaggtcc ccagtctgga gagtctgact
2581 ttaaagctag agcacgaggt ggtggccagg agccgaccaa ccccacaaga ctatgagatg
2641 cgagtatccc cctctgatac tacccctctg gtttcccgga gtgttccacc agtcaaactg
2701 gaggatgagg atgattcgga ctctgagctg gacttgagca agctgtcacc atcttcttct
2761 tcttcctcat cctcatccag ctccagctcc agcactgatg agagtgagga tgagaaggaa
2821 gagaagctaa ctgaccagtc ccgctcaaag ctctatgatg aagagagtct cctgtccctc
2881 actatgtccc aagatggatt cccaaatgaa gatggagaac aaatgacccc tgagcttctg
2941 ctactgcagg aaagacaaag agcctctgag tggcccaagg atcgtgtcct gataaaccgt
3001 attgacctcg tctgccaggc tgtactctca gggaagtggc cttctagccg taggagccag
3061 gaaatggtaa caggaggaat tttggggcca ggcaaccact tgctagacag tccctcattg
3121 actcctggag aatatggtga ctctccagtc cccacaccac gaagtagtag tgcagcttcc
3181 atggcagagg aggaagcatc tgcagtcagc acagcggcag cccagttcac caaacttcgc
3241 cgaggcatgg atgaaaagga gtttacagtt caaatcaaag atgaggaagg attgaagtta
3301 acattccaga agcacaagtt gatggcgaat ggagtaatgg gagatggaca tccactgttt
3361 cataagaaga aggggaacag aaagaagcta gtagagctgg aggtggagtg catggaagag
3421 cctaatcacc ttgatgtgga cctggagacc cggatccctg tcatcaataa ggtggatggt
3481 actttgctgg tgggtgagga tgcccctcgc cgggctgaac tggagatgtg gttacagggt
3541 catccagagt ttgctgttga tccccgattt ctagcgtata tggaggatcg cagaaaacag
3601 aagtggcaaa gatgtaaaaa aaataataag gcagaattga actgtttggg aatggaacca
3661 gtacagacag ctaactctag aaatgggaaa aagggtcatc acactgaaac ggtgttcaac
3721 cgggttttgc cagggcctat tgcaccagag agcagcaaga agcgggcccg taggatgcga
3781 ccagaccttt ctaagatgat ggccctcatg cagggtggaa gcactgggtc tctatctctg
3841 cataacacgt tccaacacag cagtagtggc ctacagtctg tgtcatcttt gggtcacagc
3901 agtgccactt ctgcatcttt gccttttatg ccatttgtga tgggtggtgc accatcatcc
3961 cctcatgtag actccagcac catgcttcat caccaccacc accaccccca cccccaccat
4021 caccaccatc accatccagg cttgagagcc cctggctacc cctcttcacc agtgactacc
4081 gcctctggta ctaccttgcg gttgccacca ctgcaacctg aggaggatga cgatgaggat
4141 gaagaagatg atgatgactt atctcagggc tatgatagct cagaaaggga cttctcactc
4201 attgatgatc ctatgatgcc agctaactca gactccagtg aagatgctga tgactgtaca
4261 gactacaaag accatgacgg tgattataaa gatcatgaca tcgactacaa ggatgacgat
4321 gacaagtaac tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt
4381 ccttgaccct ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat
4441 cgcattgtct gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg
4501 gggaggattg ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcacgtg
4561 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
4621 cgctcgctca ctgaggccgc ccgggcaaag cccgggcgtc gggcgacctt tggtcgcccg
4681 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
4741 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
4801 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4861 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4921 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4981 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
5041 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
5101 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
5161 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
5221 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
5281 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
5341 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
5401 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
5461 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
5521 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
5581 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
5641 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
5701 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
5761 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
5821 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
5881 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
5941 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
6001 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
6061 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
6121 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
6181 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
6241 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
6301 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
6361 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
6421 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
6481 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
6541 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
6601 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
6661 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
6721 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
6781 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
6841 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
6901 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
6961 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
7021 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
7081 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
7141 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
7201 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
7261 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
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