| 出品公司: | -- |
|---|---|
| 载体名称: | pHAGE-CMV-MCS-IRES-ZsGreen |
| 质粒类型: | 慢病毒载体 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | CMV |
| 载体大小: | 7742 bp |
| 5' 测序引物及序列: | -- |
| 3' 测序引物及序列: | -- |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | -- |
| 克隆菌株: | -- |
| 宿主细胞（系）: | -- |
| 备注: | 具有内部CMV启动子的慢病毒载体，具有IRES-ZsGreen（IRES荧光标签）；安培电阻 限制酶克隆。 |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pHAGE-CMV-MCS-IRES-ZsGreen载体质粒基本信息
pHAGE-CMV-MCS-IRES-ZsGreen质粒图谱载体图谱和pHAGE-CMV-MCS-IRES-ZsGreen载体序列质粒序列多克隆位点信息
pHAGE-CMV-MCS-IRES-ZsGreen质粒载体简介
pHAGE-CMV-MCS-IRES-ZsGreen质粒序列载体序列
LOCUS pHAGE-CMV-MCS-IRES-ZsGreen 7742 bp ds-DNA circular SYN 25-OCT-2021
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7742)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Monday, Oct 25, 2021 from SnapGene Viewer 5.1.4.1 to Vector
NTI(R) format
https://www.biofeng.com
COMMENT VNTDATE|964310400|
COMMENT VNTDBDATE|964310400|
COMMENT VNTNAME|pHAGE-CMV-MCS-IRES-ZsGreen|
FEATURES Location/Qualifiers
LTR 1..634
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 681..806
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 1303..1536
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 1721..1765
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
misc_feature 2058..2175
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 2227..2530
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 2531..2734
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
misc_feature 2842..3409
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 2859..3411
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
CDS 3423..4118
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
misc_feature 4132..4720
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
CDS complement(4603..4614)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
LTR 4795..5028
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
promoter 5233..5337
/gene="bla"
/label=AmpR promoter
CDS 5338..6198
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
rep_origin 6369..6957
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
protein_bind 7368..7389
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 7404..7434
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 7442..7458
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 7466..7482
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 tggaagggct aattcactcc caaagaagac aagatatcct tgatctgtgg atctaccaca
61 cacaaggcta cttccctgat tagcagaact acacaccagg gccaggggtc agatatccac
121 tgacctttgg atggtgctac aagctagtac cagttgagcc agataaggta gaagaggcca
181 ataaaggaga gaacaccagc ttgttacacc ctgtgagcct gcatgggatg gatgacccgg
241 agagagaagt gttagagtgg aggtttgaca gccgcctagc atttcatcac gtggcccgag
301 agctgcatcc ggagtacttc aagaactgct gatatcgagc ttgctacaag ggactttccg
361 ctggggactt tccagggagg cgtggcctgg gcgggactgg ggagtggcga gccctcagat
421 cctgcatata agcagctgct ttttgcctgt actgggtctc tctggttaga ccagatctga
481 gcctgggagc tctctggcta actagggaac ccactgctta agcctcaata aagcttgcct
541 tgagtgcttc aagtagtgtg tgcccgtctg ttgtgtgact ctggtaacta gagatccctc
601 agaccctttt agtcagtgtg gaaaatctct agcagtggcg cccgaacagg gacttgaaag
661 cgaaagggaa accagaggag ctctctcgac gcaggactcg gcttgctgaa gcgcgcacgg
721 caagaggcga ggggcggcga ctggtgagta cgccaaaaat tttgactagc ggaggctaga
781 aggagagaga tgggtgcgag agcgtcagta ttaagcgggg gagaattaga tcgcgatggg
841 aaaaaattcg gttaaggcca gggggaaaga aaaaatataa attaaaacat atagtatggg
901 caagcaggga gctagaacga ttcgcagtta atcctggcct gttagaaaca tcagaaggct
961 gtagacaaat actgggacag ctacaaccat cccttcagac aggatcagaa gaacttagat
1021 cattatataa tacagtagca accctctatt gtgtgcatca aaggatagag ataaaagaca
1081 ccaaggaagc tttagacaag atagaggaag agcaaaacaa aagtaagacc accgcacagc
1141 aagcggccgg ccgctgatct tcagacctgg aggaggagat atgagggaca attggagaag
1201 tgaattatat aaatataaag tagtaaaaat tgaaccatta ggagtagcac ccaccaaggc
1261 aaagagaaga gtggtgcaga gagaaaaaag agcagtggga ataggagctt tgttccttgg
1321 gttcttggga gcagcaggaa gcactatggg cgcagcgtca atgacgctga cggtacaggc
1381 cagacaatta ttgtctggta tagtgcagca gcagaacaat ttgctgaggg ctattgaggc
1441 gcaacagcat ctgttgcaac tcacagtctg gggcatcaag cagctccagg caagaatcct
1501 ggctgtggaa agatacctaa aggatcaaca gctcctgggg atttggggtt gctctggaaa
1561 actcatttgc accactgctg tgccttggaa tgctagttgg agtaataaat ctctggaaca
1621 gatttggaat cacacgacct ggatggagtg ggacagagaa attaacaatt acacaagctt
1681 aatacactcc ttaattgaag aatcgcaaaa ccagcaagaa aagaatgaac aagaattatt
1741 ggaattagat aaatgggcaa gtttgtggaa ttggtttaac ataacaaatt ggctgtggta
1801 tataaaatta ttcataatga tagtaggagg cttggtaggt ttaagaatag tttttgctgt
1861 actttctata gtgaatagag ttaggcaggg atattcacca ttatcgtttc agacccacct
1921 cccaaccccg aggggacccg acaggcccga aggaatagaa gaagaaggtg gagagagaga
1981 cagagacaga tccattcgat tagtgaacgg atctcgacgg tatcgccgaa ttcacaaatg
2041 gcagtattca tccacaattt taaaagaaaa ggggggattg gggggtacag tgcaggggaa
2101 agaatagtag acataatagc aacagacata caaactaaag aattacaaaa acaaattaca
2161 aaaattcaaa attttcgggt ttattacagg gacagcagag atccagtttg gactagtgga
2221 gttccgcgtt acataactta cggtaaatgg cccgcctggc tgaccgccca acgacccccg
2281 cccattgacg tcaataatga cgtatgttcc catagtaacg ccaataggga ctttccattg
2341 acgtcaatgg gtggagtatt tacggtaaac tgcccacttg gcagtacatc aagtgtatca
2401 tatgccaagt acgcccccta ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc
2461 ccagtacatg accttatggg actttcctac ttggcagtac atctacgtat tagtcatcgc
2521 tattaccatg gtgatgcggt tttggcagta catcaatggg cgtggatagc ggtttgactc
2581 acggggattt ccaagtctcc accccattga cgtcaatggg agtttgtttt ggcaccaaaa
2641 tcaacgggac tttccaaaat gtcgtaacaa ctccgcccca ttgacgcaaa tgggcggtag
2701 gcgtgtacgg tgggaggtct atataagcag agctcgttta gtgaaccgtc agatcgcctg
2761 gagacgccat ccacgctgtt ttgacctcca tagaagacac cggcggccgc accggtctgc
2821 agctagctcg agtctagagg atccctcccc cccccctaac gttactggcc gaagccgctt
2881 ggaataaggc cggtgtgcgt ttgtctatat gttattttcc accatattgc cgtcttttgg
2941 caatgtgagg gcccggaaac ctggccctgt cttcttgacg agcattccta ggggtctttc
3001 ccctctcgcc aaaggaatgc aaggtctgtt gaatgtcgtg aaggaagcag ttcctctgga
3061 agcttcttga agacaaacaa cgtctgtagc gaccctttgc aggcagcgga accccccacc
3121 tggcgacagg tgcctctgcg gccaaaagcc acgtgtataa gatacacctg caaaggcggc
3181 acaaccccag tgccacgttg tgagttggat agttgtggaa agagtcaaat ggctctcctc
3241 aagcgtattc aacaaggggc tgaaggatgc ccagaaggta ccccattgta tgggatctga
3301 tctggggcct cggtgcacat gctttacatg tgtttagtcg aggttaaaaa aacgtctagg
3361 ccccccgaac cacggggacg tggttttcct ttgaaaaaca cgatgataat atggccacac
3421 atatggccca gtccaagcac ggcctgacca aggagatgac catgaagtac cgcatggagg
3481 gctgcgtgga cggccacaag ttcgtgatca ccggcgaggg catcggctac cccttcaagg
3541 gcaagcaggc catcaacctg tgcgtggtgg agggcggccc cttgcccttc gccgaggaca
3601 tcttgtccgc cgccttcatg tacggcaacc gcgtgttcac cgagtacccc caggacatcg
3661 tcgactactt caagaactcc tgccccgccg gctacacctg ggaccgctcc ttcctgttcg
3721 aggacggcgc cgtgtgcatc tgcaacgccg acatcaccgt gagcgtggag gagaactgca
3781 tgtaccacga gtccaagttc tacggcgtga acttccccgc cgacggcccc gtgatgaaga
3841 agatgaccga caactgggag ccctcctgcg agaagatcat ccccgtgccc aagcagggca
3901 tcttgaaggg cgacgtgagc atgtacctgc tgctgaagga cggtggccgc ttgcgctgcc
3961 agttcgacac cgtgtacaag gccaagtccg tgccccgcaa gatgcccgac tggcacttca
4021 tccagcacaa gctgacccgc gaggaccgca gcgacgccaa gaaccagaag tggcacctga
4081 ccgagcacgc catcgcctcc ggctccgcct tgccctgaat cgatagatcc taatcaacct
4141 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
4201 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
4261 attttctcct ccttgtataa atcctggttg ctgtctcttt atgaggagtt gtggcccgtt
4321 gtcaggcaac gtggcgtggt gtgcactgtg tttgctgacg caacccccac tggttggggc
4381 attgccacca cctgtcagct cctttccggg actttcgctt tccccctccc tattgccacg
4441 gcggaactca tcgccgcctg ccttgcccgc tgctggacag gggctcggct gttgggcact
4501 gacaattccg tggtgttgtc ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt
4561 gccacctgga ttctgcgcgg gacgtccttc tgctacgtcc cttcggccct caatccagcg
4621 gaccttcctt cccgcggcct gctgccggct ctgcggcctc ttccgcgtct tcgccttcgc
4681 cctcagacga gtcggatctc cctttgggcc gcctccccgc ctgagatcct ttaagaccaa
4741 tgacttacaa ggcagctgta gatcttagcc actttttaaa agaaaagggg ggactggaag
4801 ggctaattca ctcccaacga agacaagatc tgctttttgc ttgtactggg tctctctggt
4861 tagaccagat ctgagcctgg gagctctctg gctaactagg gaacccactg cttaagcctc
4921 aataaagctt gccttgagtg cttcaagtag tgtgtgcccg tctgttgtgt gactctggta
4981 actagagatc cctcagaccc ttttagtcag tgtggaaaat ctctagcagt agtagttcat
5041 gtcatcttat tattcagtat ttataacttg caaagaaatg aatatcagag agtgagaggc
5101 ccgggttaat taaggaaagg gctagatcat tcttgaagac gaaagggcct cgtgatacgc
5161 ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
5221 cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
5281 ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
5341 agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
5401 tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
5461 gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
5521 gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
5581 gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
5641 gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
5701 agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
5761 ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
5821 cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
5881 gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
5941 cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
6001 gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
6061 ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
6121 acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
6181 ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
6241 aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
6301 aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
6361 ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
6421 ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
6481 actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc gtagttaggc
6541 caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
6601 gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
6661 ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
6721 cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
6781 cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
6841 acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
6901 ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
6961 gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
7021 tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
7081 accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
7141 cgcccaatac gcaaaccgcc tctccccgcg cgttggccga ttcattaatg cagcaagctc
7201 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
7261 tccagaagta gtgaggaggc ttttttggag gcctaggctt ttgcaaaaag ctccccgtgg
7321 cacgacaggt ttcccgactg gaaagcgggc agtgagcgca acgcaattaa tgtgagttag
7381 ctcactcatt aggcacccca ggctttacac tttatgcttc cggctcgtat gttgtgtgga
7441 attgtgagcg gataacaatt tcacacagga aacagctatg acatgattac gaatttcaca
7501 aataaagcat ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct
7561 tatcatgtct ggatcaactg gataactcaa gctaaccaaa atcatcccaa acttcccacc
7621 ccatacccta ttaccactgc caattacctg tggtttcatt tactctaaac ctgtgattcc
7681 tctgaattat tttcatttta aagaaattgt atttgttaaa tatgtactac aaacttagta
7741 gt
//
