pAAVLINK-EF1alpha-HA-5'-SCN9A

LOCUS       pAAVLINK-EF1alpha-HA-5'-SCN9A               7125 bp DNA     circular SYN 10-AUG-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7125)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7125
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..4265
                     /codon_start=1
                     /label=SCN9A
                     /translation="MAMLPPPGPQSFVHFTKQSLALIEQRIAERKSKEPKEEKKDDDEE
                     APKPSSDLEAGKQLPFIYGDIPPGMVSEPLEDLDPYYADKKTFIVLNKGKTIFRFNATP
                     ALYMLSPFSPLRRISIKILVHSLFSMLIMCTILTNCIFMTMNNPPDWTKNVEYTFTGIY
                     TFESLVKILARGFCVGEFTFLRDPWNWLDFVVIVFAYLTEFVNLGNVSALRTFRVLRAL
                     KTISVIPGLKTIVGALIQSVKKLSDVMILTVFCLSVFALIGLQLFMGNLKHKCFRNSLE
                     NNETLESIMNTLESEEDFRKYFYYLEGSKDALLCGFSTDSGQCPEGYTCVKIGRNPDYG
                     YTSFDTFSWAFLALFRLMTQDYWENLYQQTLRAAGKTYMIFFVVVIFLGSFYLINLILA
                     VVAMAYEEQNQANIEEAKQKELEFQQMLDRLKKEQEEAEAIAAAAAEYTSIRRSRIMGL
                     SESSSETSKLSSKSAKERRNRRKKKNQKKLSSGEEKGDAEKLSKSESEDSIRRKSFHLG
                     VEGHRRAHEKRLSTPNQSPLSIRGSLFSARRSSRTSLFSFKGRGRDIGSETEFADDEHS
                     IFGDNESRRGSLFVPHRPQERRSSNISQASRSPPMLPVNGKMHSAVDCNGVVSLVDGRS
                     ALMLPNGQLLPEVIIDKATSDDSGTTNQIHKKRRCSSYLLSEDMLNDPNLRQRAMSRAS
                     ILTNTVEELEESRQKCPPWWYRFAHKFLIWNCSPYWIKFKKCIYFIVMDPFVDLAITIC
                     IVLNTLFMAMEHHPMTEEFKNVLAIGNLVFTGIFAAEMVLKLIAMDPYEYFQVGWNIFD
                     SLIVTLSLVELFLADVEGLSVLRSFRLLRVFKLAKSWPTLNMLIKIIGNSVGALGNLTL
                     VLAIIVFIFAVVGMQLFGKSYKECVCKINDDCTLPRWHMNDFFHSFLIVFRVLCGEWIE
                     TMWDCMEVAGQAMCLIVYMMVMVIGNLVVLNLFLALLLSSFSSDNLTAIEEDPDANNLQ
                     IAVTRIKKGINYVKQTLREFILKAFSKKPKISREIRQAEDLNTKKENYISNHTLAEMSK
                     GHNFLKEKDKISGFGSSVDKHLMEDSDGQSFIHNPSLTVTVPIAPGESDLENMNAEELS
                     SDSDSEYSKVRLNRSSSSECSTVDNPLPGEGEEAEAEPMNSDEPEACFTDGCVRRFSCC
                     QVNIES"
     intron          4266..4306
                     /label=Splicing Donor
     misc_feature    4310..4343
                     /label=lox2272-JT1571
     repeat_region   4388..4528
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4388..4517
                     /label=AAV2 ITR
     repeat_region   4389..4529
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4603..5058
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5340..5444
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5445..6305
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6476..7064
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctat ggcaatgttg cctcccccag gacctcagag
      781 ctttgtccat ttcacaaaac agtctcttgc cctcattgaa caacgcattg ctgaaagaaa
      841 atcaaaggaa cccaaagaag aaaagaaaga tgatgatgaa gaagccccaa agccaagcag
      901 tgacttggaa gctggcaaac agctgccctt catctatggg gacattcctc ccggcatggt
      961 gtcagagccc ctggaggact tggaccccta ctatgcagac aaaaagactt tcatagtatt
     1021 gaacaaaggg aaaacaatct tccgtttcaa tgccacacct gctttatata tgctttctcc
     1081 tttcagtcct ctaagaagaa tatctattaa gattttagta cactccttat tcagcatgct
     1141 catcatgtgc actattctga caaactgcat atttatgacc atgaataacc caccggactg
     1201 gaccaaaaat gtcgagtaca cttttactgg aatatatact tttgaatcac ttgtaaaaat
     1261 ccttgcaaga ggcttctgtg taggagaatt cacttttctt cgtgacccgt ggaactggct
     1321 ggattttgtc gtcattgttt ttgcgtattt aacagaattt gtaaacctag gcaatgtttc
     1381 agctcttcga actttcagag tattgagagc tttgaaaact atttctgtaa tcccaggcct
     1441 gaagacaatt gtaggggctt tgatccagtc agtgaagaag ctttctgatg tcatgatcct
     1501 gactgtgttc tgtctgagtg tgtttgcact aattggacta cagctgttca tgggaaacct
     1561 gaagcataaa tgttttcgaa attcacttga aaataatgaa acattagaaa gcataatgaa
     1621 taccctagag agtgaagaag actttagaaa atatttttat tacttggaag gatccaaaga
     1681 tgctctcctt tgtggtttca gcacagattc aggtcagtgt ccagaggggt acacctgtgt
     1741 gaaaattggc agaaaccctg attatggcta cacgagcttt gacactttca gctgggcctt
     1801 cttagccttg tttaggctaa tgacccaaga ttactgggaa aacctttacc aacagacgct
     1861 gcgtgctgct ggcaaaacct acatgatctt ctttgtcgta gtgattttcc tgggctcctt
     1921 ttatctaata aacttgatcc tggctgtggt tgccatggca tatgaagaac agaaccaggc
     1981 aaacattgaa gaagctaaac agaaagaatt agaatttcaa cagatgttag accgtcttaa
     2041 aaaagagcaa gaagaagctg aggcaattgc agcggcagcg gctgaatata caagtattag
     2101 gagaagcaga attatgggcc tctcagagag ttcttctgaa acatccaaac tgagctctaa
     2161 aagtgctaaa gaaagaagaa acagaagaaa gaaaaagaat caaaagaagc tctccagtgg
     2221 agaggaaaag ggagatgctg agaaattgtc gaaatcagaa tcagaggaca gcatcagaag
     2281 aaaaagtttc caccttggtg tcgaagggca taggcgagca catgaaaaga ggttgtctac
     2341 ccccaatcag tcaccactca gcattcgtgg ctccttgttt tctgcaaggc gaagcagcag
     2401 aacaagtctt tttagtttca aaggcagagg aagagatata ggatctgaga ctgaatttgc
     2461 cgatgatgag cacagcattt ttggagacaa tgagagcaga aggggctcac tgtttgtgcc
     2521 ccacagaccc caggagcgac gcagcagtaa catcagccaa gccagtaggt ccccaccaat
     2581 gctgccggtg aacgggaaaa tgcacagtgc tgtggactgc aacggtgtgg tctccctggt
     2641 tgatggacgc tcagccctca tgctccccaa tggacagctt ctgccagagg tgataataga
     2701 taaggcaact tctgatgaca gcggcacgac caatcaaata cacaagaaaa ggcgttgtag
     2761 ttcctatctc ctttcagagg atatgctgaa tgatcccaac ctcagacaga gagcaatgag
     2821 tagagcaagc atattaacaa acactgtgga agaacttgaa gagtccagac aaaaatgtcc
     2881 accttggtgg tacagatttg cacacaaatt cttgatctgg aattgctctc catattggat
     2941 aaaattcaaa aagtgtatct attttattgt aatggatcct tttgtagatc ttgcaattac
     3001 catttgcata gttttaaaca cattatttat ggctatggaa caccacccaa tgactgagga
     3061 attcaaaaat gtacttgcta taggaaattt ggtctttact ggaatctttg cagctgaaat
     3121 ggtattaaaa ctgattgcca tggatccata tgagtatttc caagtaggct ggaatatttt
     3181 tgacagcctt attgtgactt taagtttagt ggagctcttt ctagcagatg tggaaggatt
     3241 gtcagttctg cgatcattca gactgctccg agtcttcaag ttggcaaaat cctggccaac
     3301 attgaacatg ctgattaaga tcattggtaa ctcagtaggg gctctaggta acctcacctt
     3361 agtgttggcc atcatcgtct tcatttttgc tgtggtcggc atgcagctct ttggtaagag
     3421 ctacaaagaa tgtgtctgca agatcaatga tgactgtacg ctcccacggt ggcacatgaa
     3481 cgacttcttc cactccttcc tgattgtgtt ccgcgtgctg tgtggagagt ggatagagac
     3541 catgtgggac tgtatggagg tcgctggtca agctatgtgc cttattgttt acatgatggt
     3601 catggtcatt ggaaacctgg tggtcctaaa cctatttctg gccttattat tgagctcatt
     3661 tagttcagac aatcttacag caattgaaga agaccctgat gcaaacaacc tccagattgc
     3721 agtgactaga attaaaaagg gaataaatta tgtgaaacaa accttacgtg aatttattct
     3781 aaaagcattt tccaaaaagc caaagatttc cagggagata agacaagcag aagatctgaa
     3841 tactaagaag gaaaactata tttctaacca tacacttgct gaaatgagca aaggtcacaa
     3901 tttcctcaag gaaaaagata aaatcagtgg ttttggaagc agcgtggaca aacacttgat
     3961 ggaagacagt gatggtcaat catttattca caatcccagc ctcacagtga cagtgccaat
     4021 tgcacctggg gaatccgatt tggaaaatat gaatgctgag gaacttagca gtgattcgga
     4081 tagtgaatac agcaaagtga gattaaaccg gtcaagctcc tcagagtgca gcacagttga
     4141 taaccctttg cctggagaag gagaagaagc agaggctgaa cctatgaatt ccgatgagcc
     4201 agaggcctgt ttcacagatg gttgtgtacg gaggttctca tgctgccaag ttaacataga
     4261 gtcaggtaag tatcaaggtt acaagacagg tttaaggaga ccaataagct attattcgta
     4321 taggatactt tatacgaagt tattaagtta tggagaccgg tgtggcacgt gcggaccgag
     4381 cggccgcagg aacccctagt gatggagttg gccactccct ctctgcgcgc tcgctcgctc
     4441 actgaggccg ggcgaccaaa ggtcgcccga cgcccgggct ttgcccgggc ggcctcagtg
     4501 agcgagcgag cgcgcagctg cctgcagggg cgcctgatgc ggtattttct ccttacgcat
     4561 ctgtgcggta tttcacaccg catacgtcaa agcaaccata gtacgcgccc tgtagcggcg
     4621 cattaagcgc ggcgggtgtg gtggttacgc gcagcgtgac cgctacactt gccagcgcct
     4681 tagcgcccgc tcctttcgct ttcttccctt cctttctcgc cacgttcgcc ggctttcccc
     4741 gtcaagctct aaatcggggg ctccctttag ggttccgatt tagtgcttta cggcacctcg
     4801 accccaaaaa acttgatttg ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg
     4861 tttttcgccc tttgacgttg gagtccacgt tctttaatag tggactcttg ttccaaactg
     4921 gaacaacact caactctatc tcgggctatt cttttgattt ataagggatt ttgccgattt
     4981 cggtctattg gttaaaaaat gagctgattt aacaaaaatt taacgcgaat tttaacaaaa
     5041 tattaacgtt tacaatttta tggtgcactc tcagtacaat ctgctctgat gccgcatagt
     5101 taagccagcc ccgacacccg ccaacacccg ctgacgcgcc ctgacgggct tgtctgctcc
     5161 cggcatccgc ttacagacaa gctgtgaccg tctccgggag ctgcatgtgt cagaggtttt
     5221 caccgtcatc accgaaacgc gcgagacgaa agggcctcgt gatacgccta tttttatagg
     5281 ttaatgtcat gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc
     5341 gcggaacccc tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac
     5401 aataaccctg ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt
     5461 tccgtgtcgc ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag
     5521 aaacgctggt gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg
     5581 aactggatct caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa
     5641 tgatgagcac ttttaaagtt ctgctatgtg gcgcggtatt atcccgtatt gacgccgggc
     5701 aagagcaact cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag
     5761 tcacagaaaa gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa
     5821 ccatgagtga taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc
     5881 taaccgcttt tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg
     5941 agctgaatga agccatacca aacgacgagc gtgacaccac gatgcctgta gcaatggcaa
     6001 caacgttgcg caaactatta actggcgaac tacttactct agcttcccgg caacaattaa
     6061 tagactggat ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg
     6121 gctggtttat tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag
     6181 cactggggcc agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg
     6241 caactatgga tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt
     6301 ggtaactgtc agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt
     6361 aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac
     6421 gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag
     6481 atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg
     6541 tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca
     6601 gagcgcagat accaaatact gttcttctag tgtagccgta gttaggccac cacttcaaga
     6661 actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca
     6721 gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc
     6781 agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca
     6841 ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa
     6901 aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc
     6961 cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc
     7021 gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg
     7081 cctttttacg gttcctggcc ttttgctggc cttttgctca catgt
//