| 质粒名称: | pSL1343 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002433 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for a LIRP variant where amino acid residues S53, A54 and G55 were deleted from MCP(LOV2 53/54) (PhCMV-MCP(LOV2 53/54 ΔSAG)-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
PhCMV
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP(LOV2 53/54 SAG) |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1343
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1343载体质粒基本信息
pSL1343质粒图谱载体图谱和pSL1343载体序列质粒序列多克隆位点信息
pSL1343质粒载体简介
pSL1343质粒序列载体序列
LOCUS pSL1343 6204 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6204)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 30, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6204
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 880..923
/label=oSCL2210
primer_bind 886..918
/label=Oscl32-1
primer_bind 886..918
/label=oSCL1118
primer_bind 901..918
/label=Oscl42-1
regulatory 901..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 902..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 910..1062
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQS"
primer_bind complement(1004..1079)
/label=oSCL2215
misc_feature 1023^1024
/label=Feature 16
primer_bind 1024..1043
/label=oSCL2216
primer_bind 1043..1080
/label=oSCL2328
primer_bind 1043..1080
/label=oSCL2329
primer_bind 1043..1080
/label=oSCL2330
primer_bind complement(1043..1062)
/label=oSCL2327
primer_bind complement(1051..1079)
/label=oSCL2217
primer_bind 1062..1080
/label=oSCL1757
primer_bind complement(1062..1079)
/label=oSCL2219
misc_feature 1063..1473
/label=LOV2
primer_bind complement(1452..1473)
/label=oSCL1758
primer_bind 1454..1500
/label=oSCL2218
primer_bind 1454..1500
/label=oSCL2358
primer_bind 1454..1500
/label=oSCL2359
primer_bind 1454..1500
/label=oSCL2360
primer_bind 1454..1473
/label=oSCL2216
primer_bind 1454..1473
/label=oSCL2220
CDS 1474..1701
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 1474
/label=Feature 17
primer_bind complement(1575..1596)
/label=oSCL2219
primer_bind 1596..1616
/label=oSCL2220
misc_feature 1596^1597
/label=Feature 18
primer_bind complement(1681..1724)
/label=oSCL2211
primer_bind complement(1681..1710)
/label=Oscl32-2
primer_bind complement(1681..1701)
/label=oSCL093
primer_bind complement(1681..1701)
/label=oSCL396
terminator 1801..2028
/label=bGH PA
polyA_signal 1804..2028
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2074..2502
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2516..2845
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2696..2831
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2696..2773
/label=SV40 ORI
CDS 2912..3706
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2915..3706
/label=Kan/neoR
polyA_signal 3880..4001
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4046..4066)
/label=M13-rev
primer_bind complement(4050..4066)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4072..4094)
/label=LacO
protein_bind 4074..4090
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4098..4128)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4099..4128)
/label=lac
protein_bind 4143..4164
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4452..5037)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5208..6068)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(5211..5870)
/label=AmpR
promoter complement(6069..6173)
/gene="bla"
/label=AmpR promoter
promoter complement(6110..6138)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat
961 gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca
1021 cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctttggaacg tatcgaaaag
1081 aattttgtca tcacggaccc gcgtctgccc gacaatccga ttatcttcgc gtcagactct
1141 ttcttacaac tgactgagta tagtagagag gagatattgg ggcgtaactg tagatttctt
1201 caggggccag aaactgatcg ggctaccgtt cgcaagatac gtgacgcaat agacaaccag
1261 accgaggtga cggtgcagct gattaactac acaaagtctg ggaagaagtt ctggaacctg
1321 tttcatttac aacctatgag ggaccaaaaa ggtgacgttc aatatttcat cggggttcag
1381 ttagatggga ctgagcacgt gagagatgca gcagaaagag agggtgtaat gcttattaaa
1441 aaaacagccg agaatatcga cgaagccgct ggtcagaaga gaaagtatac catcaaggtg
1501 gaggtcccca aagtggctac ccagacagtg ggcggagtcg aactgcctgt cgccgcttgg
1561 aggtcctacc tgaacatgga gctcactatc ccaattttcg ctaccaattc tgactgtgaa
1621 ctcatcgtga aggcaatgca ggggctcctc aaagacggta atcctatccc ttccgccatc
1681 gccgctaact caggtatcta ctaaggatcc actagtccag tgtggtggaa ttctgcagat
1741 atccagcaca gtggcggccg ctcgagtcta gagggcccgt ttaaacccgc tgatcagcct
1801 cgactgtgcc ttctagttgc cagccatctg ttgtttgccc ctcccccgtg ccttccttga
1861 ccctggaagg tgccactccc actgtccttt cctaataaaa tgaggaaatt gcatcgcatt
1921 gtctgagtag gtgtcattct attctggggg gtggggtggg gcaggacagc aagggggagg
1981 attgggaaga caatagcagg catgctgggg atgcggtggg ctctatggct tctgaggcgg
2041 aaagaaccag ctggggctct agggggtatc cccacgcgcc ctgtagcggc gcattaagcg
2101 cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg
2161 ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc
2221 taaatcgggg gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa
2281 aacttgatta gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc
2341 ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac
2401 tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt tcggcctatt
2461 ggttaaaaaa tgagctgatt taacaaaaat ttaacgcgaa ttaattctgt ggaatgtgtg
2521 tcagttaggg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
2581 tctcaattag tcagcaacca ggtgtggaaa gtccccaggc tccccagcag gcagaagtat
2641 gcaaagcatg catctcaatt agtcagcaac catagtcccg cccctaactc cgcccatccc
2701 gcccctaact ccgcccagtt ccgcccattc tccgccccat ggctgactaa ttttttttat
2761 ttatgcagag gccgaggccg cctctgcctc tgagctattc cagaagtagt gaggaggctt
2821 ttttggaggc ctaggctttt gcaaaaagct cccgggagct tgtatatcca ttttcggatc
2881 tgatcaagag acaggatgag gatcgtttcg catgattgaa caagatggat tgcacgcagg
2941 ttctccggcc gcttgggtgg agaggctatt cggctatgac tgggcacaac agacaatcgg
3001 ctgctctgat gccgccgtgt tccggctgtc agcgcagggg cgcccggttc tttttgtcaa
3061 gaccgacctg tccggtgccc tgaatgaact gcaggacgag gcagcgcggc tatcgtggct
3121 ggccacgacg ggcgttcctt gcgcagctgt gctcgacgtt gtcactgaag cgggaaggga
3181 ctggctgcta ttgggcgaag tgccggggca ggatctcctg tcatctcacc ttgctcctgc
3241 cgagaaagta tccatcatgg ctgatgcaat gcggcggctg catacgcttg atccggctac
3301 ctgcccattc gaccaccaag cgaaacatcg catcgagcga gcacgtactc ggatggaagc
3361 cggtcttgtc gatcaggatg atctggacga agagcatcag gggctcgcgc cagccgaact
3421 gttcgccagg ctcaaggcgc gcatgcccga cggcgaggat ctcgtcgtga cccatggcga
3481 tgcctgcttg ccgaatatca tggtggaaaa tggccgcttt tctggattca tcgactgtgg
3541 ccggctgggt gtggcggacc gctatcagga catagcgttg gctacccgtg atattgctga
3601 agagcttggc ggcgaatggg ctgaccgctt cctcgtgctt tacggtatcg ccgctcccga
3661 ttcgcagcgc atcgccttct atcgccttct tgacgagttc ttctgagcgg gactctgggg
3721 ttcgaaatga ccgaccaagc gacgcccaac ctgccatcac gagatttcga ttccaccgcc
3781 gccttctatg aaaggttggg cttcggaatc gttttccggg acgccggctg gatgatcctc
3841 cagcgcgggg atctcatgct ggagttcttc gcccacccca acttgtttat tgcagcttat
3901 aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
3961 cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg tataccgtcg
4021 acctctagct agagcttggc gtaatcatgg tcatagctgt ttcctgtgtg aaattgttat
4081 ccgctcacaa ttccacacaa catacgagcc ggaagcataa agtgtaaagc ctggggtgcc
4141 taatgagtga gctaactcac attaattgcg ttgcgctcac tgcccgcttt ccagtcggga
4201 aacctgtcgt gccagctgca ttaatgaatc ggccaacgcg cggggagagg cggtttgcgt
4261 attgggcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
4321 cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
4381 gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
4441 ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
4501 agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
4561 tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
4621 ccttcgggaa gcgtggcgct ttctcatagc tcacgctgta ggtatctcag ttcggtgtag
4681 gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
4741 ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
4801 gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
4861 aagtggtggc ctaactacgg ctacactaga agaacagtat ttggtatctg cgctctgctg
4921 aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
4981 ggtagcggtt tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa
5041 gatcctttga tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg
5101 attttggtca tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga
5161 agttttaaat caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta
5221 atcagtgagg cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc
5281 cccgtcgtgt agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg
5341 ataccgcgag acccacgctc accggctcca gatttatcag caataaacca gccagccgga
5401 agggccgagc gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt
5461 tgccgggaag ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt
5521 gctacaggca tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc
5581 caacgatcaa ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc
5641 ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca
5701 gcactgcata attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag
5761 tactcaacca agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg
5821 tcaatacggg ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa
5881 cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa
5941 cccactcgtg cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga
6001 gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga
6061 atactcatac tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg
6121 agcggataca tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt
6181 ccccgaaaag tgccacctga cgtc
//
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