| 质粒名称: | Ai6 |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 22798 |
| 存储实验室: | Hongkui Zeng |
|
载体骨架基本信息 |
|
| 载体名称: | Rosa26 Targeting Vector |
| 载体出品公司: | -- |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 9862 bp |
| 完整质粒大小: | -- |
| 载体修饰: | -- |
| 载体类型: | Mouse Targeting |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | CAG-Floxed ZsGreen |
| 物种来源: | n/a |
| 插入大小: | 6319 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | -- |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | PacI(未破坏) |
| 3' 克隆位点: | AscI (未破坏) |
| 5' 测序引物 | n/a |
| 3' 测序引物 | n/a |
|
文献引用方法 |
|
| 材料和方法部分: | Ai6 was a gift from Hongkui Zeng (Addgene plasmid # 22798 ; http://n2t.net/addgene:22798 ; RRID:Addgene_22798) |
| 参考文献部分: | A robust and high-throughput Cre reporting and characterization system for the whole mouse brain. Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL, Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H. Nat Neurosci. 2010 Jan . 13(1):133-40. 10.1038/nn.2467 PubMed 20023653 |
买家导航
Ai6载体质粒基本信息
Ai6质粒图谱载体图谱和Ai6载体序列质粒序列多克隆位点信息
Ai6质粒载体简介
Ai6质粒序列载体序列
LOCUS Ai6 16015 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS Ai6
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 16015)
AUTHORS Madisen L, Zwingman TA, Sunkin SM, Oh SW, Zariwala HA, Gu H, Ng LL,
Palmiter RD, Hawrylycz MJ, Jones AR, Lein ES, Zeng H
TITLE A robust and high-throughput Cre reporting and characterization
system for the whole mouse brain.
JOURNAL Nat Neurosci. 2010 Jan . 13(1):133-40.
PUBMED 20023653
REFERENCE 2 (bases 1 to 16015)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..16015
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 947..1250
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 1252..1529
/label=chicken beta-actin promoter
intron 1530..2546
/label=chimeric intron
/note="chimera between introns from chicken beta-actin and
rabbit beta-globin"
primer_bind 2470..2492
/label=pCAGGS-5
/note="Chimeric intron in CAG promoter, forward primer"
primer_bind 2554..2573
/label=pCAG-F
/note="Rabbit beta-globin intron, for pCAG plasmids,
forward primer"
protein_bind 2622..2655
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
protein_bind complement(2679..2712)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
polyA_signal 2879..3013
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3133..3267
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
polyA_signal 3387..3521
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
protein_bind complement(3550..3583)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
regulatory 3644..3653
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 3650..4345
/codon_start=1
/product="Zoanthus green fluorescent protein"
/label=ZsGreen1
/note="mammalian codon-optimized"
/translation="MAQSKHGLTKEMTMKYRMEGCVDGHKFVITGEGIGYPFKGKQAIN
LCVVEGGPLPFAEDILSAAFMYGNRVFTEYPQDIVDYFKNSCPAGYTWDRSFLFEDGAV
CICNADITVSVEENCMYHESKFYGVNFPADGPVMKKMTDNWEPSCEKIIPVPKQGILKG
DVSMYLLLKDGGRLRCQFDTVYKAKSVPRKMPDWHFIQHKLTREDRSDAKNQKWHLTEH
AIASGSALP"
misc_feature 4393..4981
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(4446..4466)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(4864..4875)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
primer_bind complement(4984..5000)
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4985..5001)
/label=pBluescriptKS
/note="For pBluescript vector"
polyA_signal 5008..5215
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind 5223..5256
/label=attB
/bound_moiety="phage phi-C31 integrase"
/note="minimal attB site for the phi-C31 integrase (Groth
et al., 2000)"
promoter 5278..5777
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
protein_bind 5797..5830
/label=FRT (minimal)
/bound_moiety="FLP recombinase from the Saccharomyces
cerevisiae 2u plasmid"
/note="supports FLP-mediated excision but not integration
(Turan and Bode, 2011)"
regulatory 5831..5840
/regulatory_class="other"
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
CDS 5837..6640
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MGSAIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQ
GRPVLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQD
LLSSHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDE
EHQGLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQ
DIALATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(5900..5919)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 6510..6529
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
polyA_signal 6650..7111
/label=PGK poly(A) signal
/note="mouse phosphoglycerate kinase 1 polyadenylation
signal"
misc_feature 7177..11517
/label=Rosa26 right arm
/note="right homology arm from intron 1 of the mouse Rosa26
gene (Soriano, 1999)"
promoter 11567..12066
/label=PGK promoter
/note="mouse phosphoglycerate kinase 1 promoter"
CDS 12093..12665
/codon_start=1
/gene="Corynebacterium diphtheriae tox"
/product="diphtheria toxin fragment A"
/label=DTA
/translation="DDVVDSSKSFVMENFSSYHGTKPGYVDSIQKGIQKPKSGTQGNYD
DDWKGFYSTDNKYDAAGYSVDNENPLSGKAGGVVKVTYPGLTKVLALKVDNAETIKKEL
GLSLTEPLMEQVGTEEFIKRFGDGASRVVLSLPFAEGSSSVEYINNWEQAKALSVELEI
NFETRGKRGQDAMYEYMAQACAGNRVRR"
intron 12742..12807
/label=small t intron
/note="SV40 (simian virus 40) small t antigen intron"
polyA_signal 12881..13105
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
primer_bind complement(13167..13187)
/label=T3
/note="T3 promoter, forward primer"
promoter complement(13167..13185)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(13206..13222)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(13206..13222)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(13219..13241)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 13230..13246
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(13254..13284)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 13299..13320
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(13437..13454)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(13608..14196)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(13688..13707)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(14367..15227)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 14990..15009
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(15228..15332)
/gene="bla"
/label=AmpR promoter
rep_origin 15359..15814
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(15446..15465)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 15656..15677
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 15940..15962
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 15954..15971
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 15955..15971
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 15981..16000
/label=T7
/note="T7 promoter, forward primer"
promoter 15981..15999
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
1 ccgcggcagg ccctccgagc gtggtggagc cgttctgtga gacagccggg tacgagtcgt
61 gacgctggaa ggggcaagcg ggtggtgggc aggaatgcgg tccgccctgc agcaaccgga
121 gggggaggga gaagggagcg gaaaagtctc caccggacgc ggccatggct cggggggggg
181 gggggggagg gagggtcagc gaaagtagct cgcgcgcgag cggccgccca ccctcccctt
241 cctctggggg agtcgtttta cccgccgccg gccgggcctc gtcgtctgat tggctctcgg
301 ggcccagaaa actggccctt gccattggct cgtgttcgtg caagttgagt ccatccgccg
361 gccagcgggg gcggcgagga ggcgctccca ggttccggcc ctcccctcgg ccccgcgccg
421 cagagtctgg ccgcgcgccc ctgcgcaacg tggcaggaag cgcgcgctgg gggcggggac
481 gggcagtagg gctgagcggc tgcggggcgg gtgcaagcac gtttccgact tgagttgcct
541 caagaggggc gtgctgagcc agacctccat cgcgcactcc ggggagtgga gggaaggagc
601 gagggctcag ttgggctgtt ttggaggcag gaagcacttg ctctcccaaa gtcgctctga
661 gttgttatca gtaagggagc tgcagtggag taggcgggga gaaggccgca cccttctccg
721 gaggggggag gggagtgttg caataccttt ctgggagttc tctgctgcct cctggcttct
781 gaggaccgcc ctgggcctgg gagaatccct tccccctctt ccctcgtgat ctgcaactcc
841 agtctttcta gccttaatta accgtttaaa caattctgca ggaatctagt tattaatagt
901 aatcaattac ggggtcatta gttcatagcc catatatgga gttccgcgtt acataactta
961 cggtaaatgg cccgcctggc tgaccgccca acgacccccg cccattgacg tcaataatga
1021 cgtatgttcc catagtaacg ccaataggga ctttccattg acgtcaatgg gtggagtatt
1081 tacggtaaac tgcccacttg gcagtacatc aagtgtatca tatgccaagt acgcccccta
1141 ttgacgtcaa tgacggtaaa tggcccgcct ggcattatgc ccagtacatg accttatggg
1201 actttcctac ttggcagtac atctacgtat tagtcatcgc tattaccatg gtcgaggtga
1261 gccccacgtt ctgcttcact ctccccatct cccccccctc cccaccccca attttgtatt
1321 tatttatttt ttaattattt tgtgcagcga tgggggcggg gggggggggg gggcgcgcgc
1381 caggcggggc ggggcggggc gaggggcggg gcggggcgag gcggagaggt gcggcggcag
1441 ccaatcagag cggcgcgctc cgaaagtttc cttttatggc gaggcggcgg cggcggcggc
1501 cctataaaaa gcgaagcgcg cggcgggcgg gagtcgctgc gcgctgcctt cgccccgtgc
1561 cccgctccgc cgccgcctcg cgccgcccgc cccggctctg actgaccgcg ttactcccac
1621 aggtgagcgg gcgggacggc ccttctcctc cgggctgtaa ttagcgcttg gtttaatgac
1681 ggcttgtttc ttttctgtgg ctgcgtgaaa gccttgaggg gctccgggag ggccctttgt
1741 gcggggggag cggctcgggg ggtgcgtgcg tgtgtgtgtg cgtggggagc gccgcgtgcg
1801 gctccgcgct gcccggcggc tgtgagcgct gcgggcgcgg cgcggggctt tgtgcgctcc
1861 gcagtgtgcg cgaggggagc gcggccgggg gcggtgcccc gcggtgcggg gggggctgcg
1921 aggggaacaa aggctgcgtg cggggtgtgt gcgtgggggg gtgagcaggg ggtgtgggcg
1981 cgtcggtcgg gctgcaaccc cccctgcacc cccctccccg agttgctgag cacggcccgg
2041 cttcgggtgc ggggctccgt acggggcgtg gcgcggggct cgccgtgccg ggcggggggt
2101 ggcggcaggt gggggtgccg ggcggggcgg ggccgcctcg ggccggggag ggctcggggg
2161 aggggcgcgg cggcccccgg agcgccggcg gctgtcgagg cgcggcgagc cgcagccatt
2221 gccttttatg gtaatcgtgc gagagggcgc agggacttcc tttgtcccaa atctgtgcgg
2281 agccgaaatc tgggaggcgc cgccgcaccc cctctagcgg gcgcggggcg aagcggtgcg
2341 gcgccggcag gaaggaaatg ggcggggagg gccttcgtgc gtcgccgcgc cgccgtcccc
2401 ttctccctct ccagcctcgg ggctgtccgc ggggggacgg ctgccttcgg gggggacggg
2461 gcagggcggg gttcggcttc tggcgtgtga ccggcggctc tagagcctct gctaaccatg
2521 ttcatgcctt cttctttttc ctacagctcc tgggcaacgt gctggttatt gtgctgtctc
2581 atcattttgg caaagaattg atttgatacc gcgggcccta agaagttcct attctctaga
2641 aagtatagga acttcgtcga catttaaatc atttaaatat aacttcgtat aatgtatgct
2701 atacgaagtt attcgcgatg aataaatgaa agcttgcaga tctgcgactc tagaggatct
2761 gcgactctag aggatcataa tcagccatac cacatttgta gaggttttac ttgctttaaa
2821 aaacctccca cacctccccc tgaacctgaa acataaaatg aatgcaattg ttgttgttaa
2881 cttgtttatt gcagcttata atggttacaa ataaagcaat agcatcacaa atttcacaaa
2941 taaagcattt ttttcactgc attctagttg tggtttgtcc aaactcatca atgtatctta
3001 tcatgtctgg atctgcgact ctagaggatc ataatcagcc ataccacatt tgtagaggtt
3061 ttacttgctt taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca
3121 attgttgttg ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc
3181 acaaatttca caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc
3241 atcaatgtat cttatcatgt ctggatctgc gactctagag gatcataatc agccatacca
3301 catttgtaga ggttttactt gctttaaaaa acctcccaca cctccccctg aacctgaaac
3361 ataaaatgaa tgcaattgtt gttgttaact tgtttattgc agcttataat ggttacaaat
3421 aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat tctagttgtg
3481 gtttgtccaa actcatcaat gtatcttatc atgtctggat ccccatcaag ctgatccgga
3541 acccttaata taacttcgta taatgtatgc tatacgaagt tattaggtcc ctcgacctgc
3601 agcccaagct agatcgaatt cggccggccg atatcggcgc gccgccacca tggcccagtc
3661 caagcacggc ctgaccaagg agatgaccat gaagtaccgc atggagggct gcgtggacgg
3721 ccacaagttc gtgatcaccg gcgagggcat cggctacccc ttcaagggca agcaggccat
3781 caacctgtgc gtggtggagg gcggcccctt gcccttcgcc gaggacatct tgtccgccgc
3841 cttcatgtac ggcaaccgcg tgttcaccga gtacccccag gacatcgtcg actacttcaa
3901 gaactcctgc cccgccggct acacctggga ccgctccttc ctgttcgagg acggcgccgt
3961 gtgcatctgc aacgccgaca tcaccgtgag cgtggaggag aactgcatgt accacgagtc
4021 caagttctac ggcgtgaact tccccgccga cggccccgtg atgaagaaga tgaccgacaa
4081 ctgggagccc tcctgcgaga agatcatccc cgtgcccaag cagggcatct tgaagggcga
4141 cgtgagcatg tacctgctgc tgaaggacgg tggccgcttg cgctgccagt tcgacaccgt
4201 gtacaaggcc aagtccgtgc cccgcaagat gcccgactgg cacttcatcc agcacaagct
4261 gacccgcgag gaccgcagcg acgccaagaa ccagaagtgg cacctgaccg agcacgccat
4321 cgcctccggc tccgccttgc cctgataaga tatcggccgg ccctgcagga attcgatatc
4381 aagcttatcg ataatcaacc tctggattac aaaatttgtg aaagattgac tggtattctt
4441 aactatgttg ctccttttac gctatgtgga tacgctgctt taatgccttt gtatcatgct
4501 attgcttccc gtatggcttt cattttctcc tccttgtata aatcctggtt gctgtctctt
4561 tatgaggagt tgtggcccgt tgtcaggcaa cgtggcgtgg tgtgcactgt gtttgctgac
4621 gcaaccccca ctggttgggg cattgccacc acctgtcagc tcctttccgg gactttcgct
4681 ttccccctcc ctattgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca
4741 ggggctcggc tgttgggcac tgacaattcc gtggtgttgt cggggaaatc atcgtccttt
4801 ccttggctgc tcgcctgtgt tgccacctgg attctgcgcg ggacgtcctt ctgctacgtc
4861 ccttcggccc tcaatccagc ggaccttcct tcccgcggcc tgctgccggc tctgcggcct
4921 cttccgcgtc ttcgccttcg ccctcagacg agtcggatct ccctttgggc cgcctccccg
4981 catcgatacc gtcgacctcg acctcgactg tgccttctag ttgccagcca tctgttgttt
5041 gcccctcccc cgtgccttcc ttgaccctgg aaggtgccac tcccactgtc ctttcctaat
5101 aaaatgagga aattgcatcg cattgtctga gtaggtgtca ttctattctg gggggtgggg
5161 tggggcagga cagcaagggg gaggattggg aagacaatgg caggcatgct ggggaactag
5221 tggtgccagg gcgtgccctt gggctccccg ggcgcggcgg ccgcatcgaa ttctaccggg
5281 taggggaggc gcttttccca aggcagtctg gagcatgcgc tttagcagcc ccgctgggca
5341 cttggcgcta cacaagtggc ctctggcctc gcacacattc cacatccacc ggtaggcgcc
5401 aaccggctcc gttctttggt ggccccttcg cgccaccttc tactcctccc ctagtcagga
5461 agttcccccc cgccccgcag ctcgcgtcgt gcaggacgtg acaaatggaa gtagcacgtc
5521 tcactagtct cgtgcagatg gacagcaccg ctgagcaatg gaagcgggta ggcctttggg
5581 gcagcggcca atagcagctt tgctccttcg ctttctgggc tcagaggctg ggaaggggtg
5641 ggtccggggg cgggctcagg ggcgggctca ggggcggggc gggcgcccga aggtcctccg
5701 gaggcccggc attctgcacg cttcaaaagc gcacgtctgc cgcgctgttc tcctcttcct
5761 catctccggg cctttcgacc tgcaatcgcc gctagcgaag ttcctattct ctagaaagta
5821 taggaacttc gccaccatgg gatcggccat tgaacaagat ggattgcacg caggttctcc
5881 ggccgcttgg gtggagaggc tattcggcta tgactgggca caacagacaa tcggctgctc
5941 tgatgccgcc gtgttccggc tgtcagcgca ggggcgcccg gttctttttg tcaagaccga
6001 cctgtccggt gccctgaatg aactgcagga cgaggcagcg cggctatcgt ggctggccac
6061 gacgggcgtt ccttgcgcag ctgtgctcga cgttgtcact gaagcgggaa gggactggct
6121 gctattgggc gaagtgccgg ggcaggatct cctgtcatct caccttgctc ctgccgagaa
6181 agtatccatc atggctgatg caatgcggcg gctgcatacg cttgatccgg ctacctgccc
6241 attcgaccac caagcgaaac atcgcatcga gcgagcacgt actcggatgg aagccggtct
6301 tgtcgatcag gatgatctgg acgaagagca tcaggggctc gcgccagccg aactgttcgc
6361 caggctcaag gcgcgcatgc ccgacggcga tgatctcgtc gtgacccatg gcgatgcctg
6421 cttgccgaat atcatggtgg aaaatggccg cttttctgga ttcatcgact gtggccggct
6481 gggtgtggcg gaccgctatc aggacatagc gttggctacc cgtgatattg ctgaagagct
6541 tggcggcgaa tgggctgacc gcttcctcgt gctttacggt atcgccgctc ccgattcgca
6601 gcgcatcgcc ttctatcgcc ttcttgacga gttcttctga ggggatccgc tgtaagtctg
6661 cagaaattga tgatctatta aacaataaag atgtccacta aaatggaagt ttttcctgtc
6721 atactttgtt aagaagggtg agaacagagt acctacattt tgaatggaag gattggagct
6781 acgggggtgg gggtggggtg ggattagata aatgcctgct ctttactgaa ggctctttac
6841 tattgcttta tgataatgtt tcatagttgg atatcataat ttaaacaagc aaaaccaaat
6901 taagggccag ctcattcctc ccactcatga tctatagatc tatagatctc tcgtgggatc
6961 attgtttttc tcttgattcc cactttgtgg ttctaagtac tgtggtttcc aaatgtgtca
7021 gtttcatagc ctgaagaacg agatcagcag cctctgttcc acatacactt cattctcagt
7081 attgttttgc caagttctaa ttccatcaga aagcttgcag aagatctccc caactggggt
7141 aacctttgag ttctctcagt tgggggggcg cgccggctag aagatgggcg ggagtcttct
7201 gggcaggctt aaaggctaac ctggtgtgtg ggcgttgtcc tgcaggggaa ttgaacaggt
7261 gtaaaattgg agggacaaga cttcccacag attttcggtt ttgtcgggaa gttttttaat
7321 aggggcaaat aaggaaaatg ggaggatagg tagtcatctg gggttttatg cagcaaaact
7381 acaggttatt attgcttgtg atccgcctcg gagtattttc catcgaggta gattaaagac
7441 atgctcaccc gagttttata ctctcctgct tgagatcctt actacagtat gaaattacag
7501 tgtcgcgagt tagactatgt aagcagaatt ttaatcattt ttaaagagcc cagtacttca
7561 tatccatttc tcccgctcct tctgcagcct tatcaaaagg tattttagaa cactcatttt
7621 agccccattt tcatttatta tactggctta tccaacccct agacagagca ttggcatttt
7681 ccctttcctg atcttagaag tctgatgact catgaaacca gacagattag ttacatacac
7741 cacaaatcga ggctgtagct ggggcctcaa cactgcagtt cttttataac tccttagtac
7801 actttttgtt gatcctttgc cttgatcctt aattttcagt gtctatcacc tctcccgtca
7861 ggtggtgttc cacatttggg cctattctca gtccagggag ttttacaaca atagatgtat
7921 tgagaatcca acctaaagct taactttcca ctcccatgaa tgcctctctc ctttttctcc
7981 atttataaac tgagctatta accattaatg gtttccaggt ggatgtctcc tcccccaata
8041 ttacctgatg tatcttacat attgccaggc tgatatttta agacattaaa aggtatattt
8101 cattattgag ccacatggta ttgattactg cttactaaaa ttttgtcatt gtacacatct
8161 gtaaaaggtg gttccttttg gaatgcaaag ttcaggtgtt tgttgtcttt cctgacctaa
8221 ggtcttgtga gcttgtattt tttctattta agcagtgctt tctcttggac tggcttgact
8281 catggcattc tacacgttat tgctggtcta aatgtgattt tgccaagctt cttcaggacc
8341 tataattttg cttgacttgt agccaaacac aagtaaaatg attaagcaac aaatgtattt
8401 gtgaagcttg gtttttaggt tgttgtgttg tgtgtgcttg tgctctataa taatactatc
8461 caggggctgg agaggtggct cggagttcaa gagcacagac tgctcttcca gaagtcctga
8521 gttcaattcc cagcaaccac atggtggctc acaaccatct gtaatgggat ctgatgccct
8581 cttctggtgt gtctgaagac cacaagtgta ttcacattaa ataaataaat cctccttctt
8641 cttctttttt ttttttttaa agagaatact gtctccagta gaatttactg aagtaatgaa
8701 atactttgtg tttgttccaa tatggtagcc aataatcaaa ttactcttta agcactggaa
8761 atgttaccaa ggaactaatt tttatttgaa gtgtaactgt ggacagagga gccataactg
8821 cagacttgtg ggatacagaa gaccaatgca gactttaatg tcttttctct tacactaagc
8881 aataaagaaa taaaaattga acttctagta tcctatttgt ttaaactgct agctttactt
8941 aacttttgtg cttcatctat acaaagctga aagctaagtc tgcagccatt actaaacatg
9001 aaagcaagta atgataattt tggatttcaa aaatgtaggg ccagagttta gccagccagt
9061 ggtggtgctt gcctttatgc ctttaatccc agcactctgg aggcagagac aggcagatct
9121 ctgagtttga gcccagcctg gtctacacat caagttctat ctaggatagc caggaataca
9181 cacagaaacc ctgttgggga ggggggctct gagatttcat aaaattataa ttgaagcatt
9241 ccctaatgag ccactatgga tgtggctaaa tccgtctacc tttctgatga gatttgggta
9301 ttattttttc tgtctctgct gttggttggg tcttttgaca ctgtgggctt tctttaaagc
9361 ctccttcctg ccatgtggtc tcttgtttgc tactaacttc ccatggctta aatggcatgg
9421 ctttttgcct tctaagggca gctgctgaga tttgcagcct gatttccagg gtggggttgg
9481 gaaatctttc aaacactaaa attgtccttt aatttttttt ttaaaaaatg ggttatataa
9541 taaacctcat aaaatagtta tgaggagtga ggtggactaa tattaaatga gtccctcccc
9601 tataaaagag ctattaaggc tttttgtctt atacttaact ttttttttaa atgtggtatc
9661 tttagaacca agggtcttag agttttagta tacagaaact gttgcatcgc ttaatcagat
9721 tttctagttt caaatccaga gaatccaaat tcttcacagc caaagtcaaa ttaagaattt
9781 ctgactttta atgttaattt gcttactgtg aatataaaaa tgatagcttt tcctgaggca
9841 gggtctcact atgtatctct gcctgatctg caacaagata tgtagactaa agttctgcct
9901 gcttttgtct cctgaatact aaggttaaaa tgtagtaata cttttggaac ttgcaggtca
9961 gattctttta taggggacac actaagggag cttgggtgat agttggtaaa atgtgtttca
10021 agtgatgaaa acttgaatta ttatcaccgc aacctacttt ttaaaaaaaa aagccaggcc
10081 tgttagagca tgcttaaggg atccctagga cttgctgagc acacaagagt agttacttgg
10141 caggctcctg gtgagagcat atttcaaaaa acaaggcaga caaccaagaa actacagtta
10201 aggttacctg tctttaaacc atctgcatat acacagggat attaaaatat tccaaataat
10261 atttcattca agttttcccc catcaaattg ggacatggat ttctccggtg aataggcaga
10321 gttggaaact aaacaaatgt tggttttgtg atttgtgaaa ttgttttcaa gtgatagtta
10381 aagcccatga gatacagaac aaagctgcta tttcgaggtc tcttggttta tactcagaag
10441 cacttctttg ggtttccctg cactatcctg atcatgtgct aggcctacct taggctgatt
10501 gttgttcaaa taaacttaag tttcctgtca ggtgatgtca tatgatttca tatatcaagg
10561 caaaacatgt tatatatgtt aaacatttgt acttaatgtg aaagttaggt ctttgtgggt
10621 ttgattttta attttcaaaa cctgagctaa ataagtcatt tttacatgtc ttacatttgg
10681 tggaattgta taattgtggt ttgcaggcaa gactctctga cctagtaacc ctacctatag
10741 agcactttgc tgggtcacaa gtctaggagt caagcatttc accttgaagt tgagacgttt
10801 tgttagtgta tactagttta tatgttggag gacatgttta tccagaagat attcaggact
10861 atttttgact gggctaagga attgattctg attagcactg ttagtgagca ttgagtggcc
10921 tttaggcttg aattggagtc acttgtatat ctcaaataat gctggccttt tttaaaaagc
10981 ccttgttctt tatcaccctg ttttctacat aatttttgtt caaagaaata cttgtttgga
11041 tctccttttg acaacaatag catgttttca agccatattt tttttccttt tttttttttt
11101 ttttggtttt tcgagacagg gtttctctgt atagccctgg ctgtcctgga actcactttg
11161 tagaccaggc tggcctcgaa ctcagaaatc cgcctgcctc tgcctcctga gtgccgggat
11221 taaaggcgtg caccaccacg cctggctaag ttggatattt tgttatataa ctataaccaa
11281 tactaactcc actgggtgga tttttaattc agtcagtagt cttaagtggt ctttattggc
11341 ccttcattaa aatctactgt tcactctaac agaggctgtt ggtactagtg gcacttaagc
11401 aacttcctac ggatatacta gcagattaag ggtcagggat agaaactagt ctagcgtttt
11461 gtatacctac cagctttata ctaccttgtt ctgatagaaa tatttcagga catctagctt
11521 atcgataccg tcgacggtat cgataagctt gatatcgaat tctaccgggt aggggaggcg
11581 cttttcccaa ggcagtctgg agcatgcgct ttagcagccc cgctgggcac ttggcgctac
11641 acaagtggcc tctggcctcg cacacattcc acatccaccg gtaggcgcca accggctccg
11701 ttctttggtg gccccttcgc gccaccttct actcctcccc tagtcaggaa gttccccccc
11761 gccccgcagc tcgcgtcgtg caggacgtga caaatggaag tagcacgtct cactagtctc
11821 gtgcagatgg acagcaccgc tgagcaatgg aagcgggtag gcctttgggg cagcggccaa
11881 tagcagcttt gctccttcgc tttctgggct cagaggctgg gaaggggtgg gtccgggggc
11941 gggctcaggg gcgggctcag gggcggggcg ggcgcccgaa ggtcctccgg aggcccggca
12001 ttctgcacgc ttcaaaagcg cacgtctgcc gcgctgttct cctcttcctc atctccgggc
12061 ctttcgacct gcaggtcctc gccatggatc ctgatgatgt tgttgattct tctaaatctt
12121 ttgtgatgga aaacttttct tcgtaccacg ggactaaacc tggttatgta gattccattc
12181 aaaaaggtat acaaaagcca aaatctggta cacaaggaaa ttatgacgat gattggaaag
12241 ggttttatag taccgacaat aaatacgacg ctgcgggata ctctgtagat aatgaaaacc
12301 cgctctctgg aaaagctgga ggcgtggtca aagtgacgta tccaggactg acgaaggttc
12361 tcgcactaaa agtggataat gccgaaacta ttaagaaaga gttaggttta agtctcactg
12421 aaccgttgat ggagcaagtc ggaacggaag agtttatcaa aaggttcggt gatggtgctt
12481 cgcgtgtagt gctcagcctt cccttcgctg aggggagttc tagcgttgaa tatattaata
12541 actgggaaca ggcgaaagcg ttaagcgtag aacttgagat taattttgaa acccgtggaa
12601 aacgtggcca agatgcgatg tatgagtata tggctcaagc ctgtgcagga aatcgtgtca
12661 ggcgatctct ttgtgaagga accttacttc tgtggtgtga cataattgga caaactacct
12721 acagagattt aaagctctaa ggtaaatata aaatttttaa gtgtataatg tgttaaacta
12781 ctgattctaa ttgtttgtgt attttagatt ccaacctatg gaactgatga atgggagcag
12841 tggtggaatg cagatcctag agctcgctga tcagcctcga ctgtgccttc tagttgccag
12901 ccatctgttg tttgcccctc ccccgtgcct tccttgaccc tggaaggtgc cactcccact
12961 gtcctttcct aataaaatga ggaaattgca tcgcattgtc tgagtaggtg tcattctatt
13021 ctggggggtg gggtggggca ggacagcaag ggggaggatt gggaagacaa tagcaggcat
13081 gctggggatg cggtgggctc tatggcttct gaggcggaaa gaaccagctg gggctcgagg
13141 gggggcccgg tacccagctt ttgttccctt tagtgagggt taattgcgcg cttggcgtaa
13201 tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
13261 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
13321 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
13381 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
13441 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
13501 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
13561 ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt ccataggctc
13621 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
13681 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
13741 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
13801 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
13861 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
13921 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
13981 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
14041 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
14101 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
14161 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
14221 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
14281 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
14341 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
14401 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
14461 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
14521 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
14581 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
14641 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
14701 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
14761 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
14821 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
14881 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
14941 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
15001 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
15061 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
15121 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
15181 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
15241 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
15301 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
15361 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
15421 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
15481 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
15541 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
15601 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
15661 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
15721 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
15781 gcgaatttta acaaaatatt aacgcttaca atttccattc gccattcagg ctgcgcaact
15841 gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg aaagggggat
15901 gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
15961 cgacggccag tgagcgcgcg taatacgact cactataggg cgaattggag ctcca
//
