pAAVLINKv1-pCALM1-Cre-FLNC-C-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-FLNC-C-FLAG              6735 bp DNA     circular SYN 22-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6735)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6735
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3448
                     /codon_start=1
                     /label=FLNC
                     /translation="VMGAWG*VLKAQARWTSTVRTWRTGHAKSPTAPPSPAPTSSTSSL
                     LTSTCLEAPSL*R*PARAA*RRASPGGDRHLPSPPSAAPVTSTSRSQETGSRWCLPRSA
                     *HAPSHAAATPTPARSARRSARRGAGRQSARCGWRSPPRSAGTPSLLCLGTSWAGSAWD
                     PSAASPGSRRVRPALRT*LHR*PAHRARWKPQRSSRARTAPTACALCPRKWGPIRSLSS
                     TVASTCPAAPFSSLWGRWVKVVPTRCGPEAQGWSEVWPACQPSSASGPGRLALGACPLL
                     WRVLAKRRLHLRIAKMAPAASPMSSRNQVTMRSPSSSMMSTSQTAPLWCLWPPSRMTLA
                     VSLSPASRRRGSR*TSQRPLPCS*TVPGA*LMPGCTHPRGLWRSATSLSWTVTSTPSAS
                     SPTRMASTPSMSSSTVPTSLEVPSRSALGSRARLGTQAWCQPTVLGSREALPVCHQSSS
                     *TP*MPARGPCLSPLMAPPRCSWTVGSVLRAMWSLILPWPLATTSLPSSTVAPSTSWAA
                     PSRPRSLVRGCPEATAFTKHPRFWWRL*PSPPQAGAPATAPSPSSPQMPARW*LGALGC
                     PRPSWARRTPSPWTAAKQAPT**WWACTAPRPPVRRCT*STWGTGCTMSPTLSRRKGTT
                     SSLSSGVTKVSLEAPSKSRS"
     misc_feature    1995..2000
                     /label=SDS
     misc_feature    3123..3128
                     /label=SDS
     misc_feature    3312..3317
                     /label=SDS
     CDS             3455..3520
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3524..3955
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3668..3673
                     /label=SDS
     repeat_region   3998..4138
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3998..4127
                     /label=AAV2 ITR
     rep_origin      4213..4668
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4950..5054
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5055..5915
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6086..6674
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca ggttatgggg gcttggggct
     1501 gagtattgaa ggcccaagca aggtggacat caactgtgag gacatggagg acgggacatg
     1561 caaagtcacc tactgcccca ccgagcccgg cacctacatc atcaacatca agtttgctga
     1621 caagcacgtg cctggaagcc ccttcactgt gaaggtgacc ggcgagggcc gcatgaagga
     1681 gagcatcacc cggcggagac aggcaccttc catcgccacc atcggcagca cctgtgacct
     1741 caacctcaag atcccaggaa actggttcca gatggtgtct gcccaggagc gcctgacacg
     1801 caccttcaca cgcagcagcc acacctacac ccgcacggag cgcacggaga tcagcaagac
     1861 gcggggcggg gagacaaagc gcgaggtgcg ggtggaggag tccacccagg tcggcgggga
     1921 ccccttccct gctgtgtttg gggacttcct gggccgggag cgcctgggat ccttcggcag
     1981 catcacccgg cagcaggagg gtgaggccag ctctcaggac atgactgcac aggtgaccag
     2041 cccatcgggc aaggtggaag ccgcagagat cgtcgagggc gaggacagcg cctacagcgt
     2101 gcgctttgtg ccccaggaaa tggggcccca tacggtcgct gtcaagtacc gtggccagca
     2161 cgtgcccggc agcccctttc agttcactgt ggggccgctg ggtgaaggtg gtgcccacaa
     2221 ggtgcgggcc ggaggcacag ggctggagcg aggtgtggcc ggcgtgccag ccgagttcag
     2281 catctggacc cgggaggctg gcgctggggg cctgtccatt gctgtggagg gtcctagcaa
     2341 agcggagatt gcatttgagg atcgcaaaga tggctcctgc ggcgtctcct atgtcgtcca
     2401 ggaaccaggt gactatgagg tctccatcaa gttcaatgat gagcacatcc cagacagccc
     2461 ctttgtggtg cctgtggcct ccctctcgga tgacgctcgc cgtctcactg tcaccagcct
     2521 ccaggagacg gggctcaagg tgaaccagcc agcgtccttt gccgtgcagc tgaacggtgc
     2581 ccggggcgtg attgatgccc gggtgcacac accctcgggg gctgtggagg agtgctacgt
     2641 ctctgagctg gacagtgaca agcacaccat ccgcttcatc ccccacgaga atggcgtcca
     2701 ctccatcgat gtcaagttca acggtgccca catccctgga agtcccttca agatccgcgt
     2761 tggggagcag agccaggctg gggacccagg cttggtgtca gcctacggtc ctgggctcga
     2821 gggaggcact accggtgtgt catcagagtt catcgtgaac accctgaatg ccggctcggg
     2881 ggccttgtct gtcaccattg atggcccctc caaggtgcag ctggactgtc gggagtgtcc
     2941 tgagggccat gtggtcactt atactcccat ggcccctggc aactacctca ttgccatcaa
     3001 gtacggtggc ccccagcaca tcgtgggcag ccccttcaag gccaaggtca ctggtccgag
     3061 gctgtccgga ggccacagcc ttcacgaaac atccacggtt ctggtggaga ctgtgaccaa
     3121 gtcctcctca agccggggct ccagctacag ctccatcccc aagttctcct cagatgccag
     3181 caaggtggtg actcggggcc ctgggctgtc ccaggccttc gtgggccaga agaactcctt
     3241 caccgtggac tgcagcaaag caggcaccaa catgatgatg gtgggcgtgc acggccccaa
     3301 gaccccctgt gaggaggtgt acgtgaagca catggggaac cgggtgtaca atgtcaccta
     3361 cactgtcaag gagaaagggg actacatcct cattgtcaag tggggtgacg aaagtgtccc
     3421 tggaagcccc ttcaaagtca aggtcccttg tacagactac aaagaccatg acggtgatta
     3481 taaagatcat gacatcgact acaaggatga cgatgacaag taactcgaga taatcaacct
     3541 ctggattaca aaatttgtga aagattgact ggtattctta actatgttgc tccttttacg
     3601 ctatgtggat acgctgcttt aatgcctttg tatcatgcta ttgcttcccg tatggctttc
     3661 attttctcct ccttgtataa atcctggtta gttcttgcca cggcggaact catcgccgcc
     3721 tgccttgccc gctgctggac aggggctcgg ctgttgggca ctgacaattc cgtggtgttt
     3781 atttgtgaaa tttgtgatgc tattgcttta tttgtaacca tctagcttta tttgtgaaat
     3841 ttgtgatgct attgctttat ttgtaaccat tataagctgc aataaacaag ttaacaacaa
     3901 caattgcatt cattttatgt ttcaggttca gggggagatg tgggaggttt tttaaagatc
     3961 tggagaccgg tgtggcacgt gcggaccgag cggccgcagg aacccctagt gatggagttg
     4021 gccactccct ctctgcgcgc tcgctcgctc actgaggccg ggcgaccaaa ggtcgcccga
     4081 cgcccgggct ttgcccgggc ggcctcagtg agcgagcgag cgcgcagctg cctgcagggg
     4141 cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     4201 agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     4261 gcagcgtgac cgctacactt gccagcgcct tagcgcccgc tcctttcgct ttcttccctt
     4321 cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     4381 ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     4441 cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     4501 tctttaatag tggactcttg ttccaaactg gaacaacact caactctatc tcgggctatt
     4561 cttttgattt ataagggatt ttgccgattt cggtctattg gttaaaaaat gagctgattt
     4621 aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     4681 tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     4741 ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     4801 tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     4861 agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     4921 cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     4981 tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     5041 gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     5101 cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     5161 atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     5221 agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     5281 gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     5341 ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     5401 cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     5461 ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     5521 atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     5581 gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     5641 tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     5701 gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     5761 gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     5821 tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     5881 ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     5941 tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     6001 ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     6061 ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     6121 tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     6181 ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gttcttctag
     6241 tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     6301 tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     6361 actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     6421 cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     6481 gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     6541 tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     6601 ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     6661 ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     6721 cttttgctca catgt
//