pAAVLINKv2-SCP1-Cre-UDeg3a-APC-C-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-APC-C-FLAG              6851 bp DNA     circular SYN 26-JAN-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6851)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6851
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3564
                     /codon_start=1
                     /label=APC
                     /translation="GRKVHWKLKR*NLKVKESKEEKKFIKV*LLEKFDLIQKFQAK*NS
                     PFKQTCLQSLEAGQ*FIFQEFEIAPQVQVLFLKKAHPLRLQPPKALVKVKQPPLLLEEP
                     SHL*NQN*ALLPGRHPK*VGQVKHLLDQDLEIRPLQDLPSNH*VDLYSLLAETQFPLVE
                     ME*VLLTNYLNFQGHHPLVLLQLSPQVLEKCHIHLQVDR*ANRTLPNKQVYPRMPVVFQ
                     EVSLPPKD*IR*IMVMEPIKR*NFLECLQLNQVEVNLIDQKDLY*YASQLSSKKLQAQP
                     *EENWRNLLHLNLFLHHLDQLLPLGPRHKLQF*VLPFLICLYPHIRLFRLVDGENSHLI
                     SVPL*SIMMEDQQSAMILHGLILKVLLDFQSIGQEPGNVSTANIHHPFLE*ALGEELEV
                     HLQFFLLHQNPVKKQKVRMKNM*TLFQEPNKVKKTKYPQKEHGEK*KKMNFLPQIVLLR
                     PFPQVLQMVLNQRL*FIKWHLLFLKQRMFG*ELRTVPLTILDLEDLPQVILPR*LTVFQ
                     KRQIQTLKIQKIIRQNKMWVMAVFPCVPWVWKIA*TPLFRWMPLTKKELR*NQDKIILS
                     LYQRLMKVL*WNVPHSVLAAQANTVHLVGLLLPE*LLLITTQALGKAAQIALQLGHLRS
                     QLQ*ITTQRSEIPKLTAQNPVEPKVLSAILGLTL*HL"
     misc_feature    1602..1607
                     /label=SDS
     misc_feature    2047..2052
                     /label=SDS
     CDS             3571..3636
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3640..4071
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3784..3789
                     /label=SDS
     repeat_region   4114..4254
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4114..4243
                     /label=AAV2 ITR
     rep_origin      4329..4784
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5066..5170
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5171..6031
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6202..6790
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacagggga gaaaagtaca
     1561 ttggaaacta aaaagataga atctgaaagt aaaggaatca aaggaggaaa aaaagtttat
     1621 aaaagtttga ttactggaaa agttcgatct aattcagaaa tttcaggcca aatgaaacag
     1681 ccccttcaag caaacatgcc ttcaatctct cgaggcagga caatgattca tattccagga
     1741 gttcgaaata gctcctcaag tacaagtcct gtttctaaaa aaggcccacc ccttaagact
     1801 ccagcctcca aaagccctag tgaaggtcaa acagccacca cttctcctag aggagccaag
     1861 ccatctgtga aatcagaatt aagccctgtt gccaggcaga catcccaaat aggtgggtca
     1921 agtaaagcac cttctagatc aggatctaga gattcgaccc cttcaagacc tgcccagcaa
     1981 ccattaagta gacctataca gtctcctggc cgaaactcaa tttcccctgg tagaaatgga
     2041 ataagtcctc ctaacaaatt atctcaactt ccaaggacat catcccctag tactgcttca
     2101 actaagtcct caggttctgg aaaaatgtca tatacatctc caggtagaca gatgagccaa
     2161 cagaacctta ccaaacaaac aggtttatcc aagaatgcca gtagtattcc aagaagtgag
     2221 tctgcctcca aaggactaaa tcagatgaat aatggtaatg gagccaataa aaaggtagaa
     2281 ctttctagaa tgtcttcaac taaatcaagt ggaagtgaat ctgatagatc agaaagacct
     2341 gtattagtac gccagtcaac tttcatcaaa gaagctccaa gcccaacctt aagaagaaaa
     2401 ttggaggaat ctgcttcatt tgaatctctt tctccatcat ctagaccagc ttctcccact
     2461 aggtcccagg cacaaactcc agttttaagt ccttcccttc ctgatatgtc tctatccaca
     2521 cattcgtctg ttcaggctgg tggatggcga aaactcccac ctaatctcag tcccactata
     2581 gagtataatg atggaagacc agcaaagcgc catgatattg cacggtctca ttctgaaagt
     2641 ccttctagac ttccaatcaa taggtcagga acctggaaac gtgagcacag caaacattca
     2701 tcatcccttc ctcgagtaag cacttggaga agaactggaa gttcatcttc aattctttct
     2761 gcttcatcag aatccagtga aaaagcaaaa agtgaggatg aaaaacatgt gaactctatt
     2821 tcaggaacca aacaaagtaa agaaaaccaa gtatccgcaa aaggaacatg gagaaaaata
     2881 aaagaaaatg aattttctcc cacaaatagt acttctcaga ccgtttcctc aggtgctaca
     2941 aatggtgctg aatcaaagac tctaatttat caaatggcac ctgctgtttc taaaacagag
     3001 gatgtttggg tgagaattga ggactgtccc attaacaatc ctagatctgg aagatctccc
     3061 acaggtaata ctcccccggt gattgacagt gtttcagaaa aggcaaatcc aaacattaaa
     3121 gattcaaaag ataatcaggc aaaacaaaat gtgggtaatg gcagtgttcc catgcgtacc
     3181 gtgggtttgg aaaatcgcct gaactccttt attcaggtgg atgcccctga ccaaaaagga
     3241 actgagataa aaccaggaca aaataatcct gtccctgtat cagagactaa tgaaagttct
     3301 atagtggaac gtaccccatt cagttctagc agctcaagca aacacagttc acctagtggg
     3361 actgttgctg ccagagtgac tccttttaat tacaacccaa gccctaggaa aagcagcgca
     3421 gatagcactt cagctcggcc atctcagatc ccaactccag tgaataacaa cacaaagaag
     3481 cgagattcca aaactgacag cacagaatcc agtggaaccc aaagtcctaa gcgccattct
     3541 gggtcttacc ttgtgacatc tgtttgtaca gactacaaag accatgacgg tgattataaa
     3601 gatcatgaca tcgactacaa ggatgacgat gacaagtaac tcgagataat caacctctgg
     3661 attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct tttacgctat
     3721 gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg gctttcattt
     3781 tctcctcctt gtataaatcc tggttagttc ttgccacggc ggaactcatc gccgcctgcc
     3841 ttgcccgctg ctggacaggg gctcggctgt tgggcactga caattccgtg gtgtttattt
     3901 gtgaaatttg tgatgctatt gctttatttg taaccatcta gctttatttg tgaaatttgt
     3961 gatgctattg ctttatttgt aaccattata agctgcaata aacaagttaa caacaacaat
     4021 tgcattcatt ttatgtttca ggttcagggg gagatgtggg aggtttttta aagatctgga
     4081 gaccggtgtg gcacgtgcgg accgagcggc cgcaggaacc cctagtgatg gagttggcca
     4141 ctccctctct gcgcgctcgc tcgctcactg aggccgggcg accaaaggtc gcccgacgcc
     4201 cgggctttgc ccgggcggcc tcagtgagcg agcgagcgcg cagctgcctg caggggcgcc
     4261 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata cgtcaaagca
     4321 accatagtac gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag
     4381 cgtgaccgct acacttgcca gcgccttagc gcccgctcct ttcgctttct tcccttcctt
     4441 tctcgccacg ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt
     4501 ccgatttagt gctttacggc acctcgaccc caaaaaactt gatttgggtg atggttcacg
     4561 tagtgggcca tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt
     4621 taatagtgga ctcttgttcc aaactggaac aacactcaac tctatctcgg gctattcttt
     4681 tgatttataa gggattttgc cgatttcggt ctattggtta aaaaatgagc tgatttaaca
     4741 aaaatttaac gcgaatttta acaaaatatt aacgtttaca attttatggt gcactctcag
     4801 tacaatctgc tctgatgccg catagttaag ccagccccga cacccgccaa cacccgctga
     4861 cgcgccctga cgggcttgtc tgctcccggc atccgcttac agacaagctg tgaccgtctc
     4921 cgggagctgc atgtgtcaga ggttttcacc gtcatcaccg aaacgcgcga gacgaaaggg
     4981 cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc
     5041 aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     5101 ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     5161 aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt
     5221 ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca
     5281 gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag
     5341 ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc
     5401 ggtattatcc cgtattgacg ccgggcaaga gcaactcggt cgccgcatac actattctca
     5461 gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt
     5521 aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct
     5581 gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt
     5641 aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga
     5701 caccacgatg cctgtagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact
     5761 tactctagct tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc
     5821 acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga
     5881 gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt
     5941 agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga
     6001 gataggtgcc tcactgatta agcattggta actgtcagac caagtttact catatatact
     6061 ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga
     6121 taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt
     6181 agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca
     6241 aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct
     6301 ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgttc ttctagtgta
     6361 gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct
     6421 aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc
     6481 aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca
     6541 gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga
     6601 aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg
     6661 aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt
     6721 cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag
     6781 cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt
     6841 tgctcacatg t
//