T7-VEE-OKS-iM

LOCUS       T7-VEE-OKS-iM               16336 bp ds-DNA     circular SYN 06-AUG-2018
DEFINITION  RNA-based iPSC generation. VEE RNA replicon 3'ORF encoding OCT4, 
            KLF4, SOX2, separated by 2A peptides followed by an IRES then c-myc 
            and a second IRES and PuroR ORF.
ACCESSION   .
VERSION     .
KEYWORDS    T7-VEE-OKS-iM
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 16336)
  AUTHORS   Yoshioka N, Gros E, Li HR, Kumar S, Deacon DC, Maron C, Muotri AR, 
            Chi NC, Fu XD, Yu BD, Dowdy SF
  TITLE     Efficient generation of human iPSCs by a synthetic self-replicative 
            RNA.
  JOURNAL   Cell Stem Cell. 2013 Aug 1;13(2):246-54. doi: 
            10.1016/j.stem.2013.06.001.
  PUBMED    23910086
REFERENCE   2  (bases 1 to 16336)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 6, 2018 from SnapGene Server 1.1.58
            http://www.snapgene.com
FEATURES             Location/Qualifiers
     source          1..16336
                     /organism="synthetic DNA construct"
                     /mol_type="other DNA"
     RBS             1535..1543
                     /label=Shine-Dalgarno sequence
                     /note="full consensus sequence for ribosome-binding sites 
                     upstream of start codons in E. coli; complementary to a 
                     region in the 3' end of the 16S rRNA (Chen et al., 1994)"
     regulatory      7586..7595
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             8678..8731
                     /codon_start=1
                     /product="2A peptide from Thosea asigna virus capsid 
                     protein"
                     /label=T2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="EGRGSLLTCGDVEENPGP"
     CDS             10154..10213
                     /codon_start=1
                     /product="2A peptide from equine rhinitis A virus 
                     polyprotein"
                     /label=E2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="QCTNYALLKLAGDVESNPGP"
     regulatory      11812..11821
                     /regulatory_class="other"
                     /note="vertebrate consensus sequence for strong initiation 
                     of translation (Kozak, 1987)"
     CDS             11818..13140
                     /codon_start=1
                     /product="human c-Myc proto-oncogene"
                     /label=c-Myc
                     /translation="MGPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPA
                     PSEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVT
                     ELLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSP
                     NPARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSD
                     SLLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESG
                     SPSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNN
                     RKCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKK
                     ATAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
     CDS             13777..14376
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     primer_bind     complement(13777..13796)
                     /label=Puro-R
                     /note="Puromycin resistance gene, reverse primer. Also 
                     called puro-variant-R"
     primer_bind     14273..14293
                     /label=Puro-F
                     /note="Puromycin resistance gene, forward primer"
     promoter        14574..14678
                     /gene="bla"
                     /label=AmpR promoter
     CDS             14679..15539
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     complement(14897..14916)
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     rep_origin      15710..16298
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     16199..16218
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     promoter        16319..16336
                     /label=T7 promoter
                     /note="promoter for bacteriophage T7 RNA polymerase"
ORIGIN
        1 atgggcggcg catgagagaa gcccagacca attacctacc caaaatggag aaagttcacg
       61 ttgacatcga ggaagacagc ccattcctca gagctttgca gcggagcttc ccgcagtttg
      121 aggtagaagc caagcaggtc actgataatg accatgctaa tgccagagcg ttttcgcatc
      181 tggcttcaaa actgatcgaa acggaggtgg acccatccga cacgatcctt gacattggaa
      241 gtgcgcccgc ccgcagaatg tattctaagc acaagtatca ttgtatctgt ccgatgagat
      301 gtgcggaaga tccggacaga ttgtataagt atgcaactaa gctgaagaaa aactgtaagg
      361 aaataactga taaggaattg gacaagaaaa tgaaggagct ggccgccgtc atgagcgacc
      421 ctgacctgga aactgagact atgtgcctcc acgacgacga gtcgtgtcgc tacgaagggc
      481 aagtcgctgt ttaccaggat gtatacgcgg ttgacggacc gacaagtctc tatcaccaag
      541 ccaataaggg agttagagtc gcctactgga taggctttga caccacccct tttatgttta
      601 agaacttggc tggagcatat ccatcatact ctaccaactg ggccgacgaa accgtgttaa
      661 cggctcgtaa cataggccta tgcagctctg acgttatgga gcggtcacgt agagggatgt
      721 ccattcttag aaagaagtat ttgaaaccat ccaacaatgt tctattctct gttggctcga
      781 ccatctacca cgagaagagg gacttactga ggagctggca cctgccgtct gtatttcact
      841 tacgtggcaa gcaaaattac acatgtcggt gtgagactat agttagttgc gacgggtacg
      901 tcgttaaaag aatagctatc agtccaggcc tgtatgggaa gccttcaggc tatgctgcta
      961 cgatgcaccg cgagggattc ttgtgctgca aagtgacaga cacattgaac ggggagaggg
     1021 tctcttttcc cgtgtgcacg tatgtgccag ctacattgtg tgaccaaatg actggcatac
     1081 tggcaacaga tgtcagtgcg gacgacgcgc aaaaactgct ggttgggctc aaccagcgta
     1141 tagtcgtcaa cggtcgcacc cagagaaaca ccaataccat gaaaaattac cttttgcccg
     1201 tagtggccca ggcatttgct aggtgggcaa aggaatataa ggaagatcaa gaagatgaaa
     1261 ggccactagg actacgagat agacagttag tcatggggtg ttgttgggct tttagaaggc
     1321 acaagataac atctatttat aagcgcccgg atacccaaac catcatcaaa gtgaacagcg
     1381 atttccactc attcgtgctg cccaggatag gcagtaacac attggagatc gggctgagaa
     1441 caagaatcag gaaaatgtta gaggagcaca aggagccgtc acctctcatt accgccgagg
     1501 acgtacaaga agctaagtgc gcagccgatg aggctaagga ggtgcgtgaa gccgaggagt
     1561 tgcgcgcagc tctaccacct ttggcagctg atgttgagga gcccactctg gaagccgatg
     1621 tcgacttgat gttacaagag gctggggccg gctcagtgga gacacctcgt ggcttgataa
     1681 aggttaccag ctacgatggc gaggacaaga tcggctctta cgctgtgctt tctccgcagg
     1741 ctgtactcaa gagtgaaaaa ttatcttgca tccaccctct cgctgaacaa gtcatagtga
     1801 taacacactc tggccgaaaa gggcgttatg ccgtggaacc ataccatggt aaagtagtgg
     1861 tgccagaggg acatgcaata cccgtccagg actttcaagc tctgagtgaa agtgccacca
     1921 ttgtgtacaa cgaacgtgag ttcgtaaaca ggtacctgca ccatattgcc acacatggag
     1981 gagcgctgaa cactgatgaa gaatattaca aaactgtcaa gcccagcgag cacgacggcg
     2041 aatacctgta cgacatcgac aggaaacagt gcgtcaagaa agaactagtc actgggctag
     2101 ggctcacagg cgagctggtg gatcctccct tccatgaatt cgcctacgag agtctgagaa
     2161 cacgaccagc cgctccttac caagtaccaa ccataggggt gtatggcgtg ccaggatcag
     2221 gcaagtctgg catcattaaa agcgcagtca ccaaaaaaga tctagtggtg agcgccaaga
     2281 aagaaaactg tgcagaaatt ataagggacg tcaagaaaat gaaagggctg gacgtcaatg
     2341 ccagaactgt ggactcagtg ctcttgaatg gatgcaaaca ccccgtagag accctgtata
     2401 ttgacgaagc ttttgcttgt catgcaggta ctctcagagc gctcatagcc attataagac
     2461 ctaaaaaggc agtgctctgc ggggatccca aacagtgcgg tttttttaac atgatgtgcc
     2521 tgaaagtgca ttttaaccac gagatttgca cacaagtctt ccacaaaagc atctctcgcc
     2581 gttgcactaa atctgtgact tcggtcgtct caaccttgtt ttacgacaaa aaaatgagaa
     2641 cgacgaatcc gaaagagact aagattgtga ttgacactac cggcagtacc aaacctaagc
     2701 aggacgatct cattctcact tgtttcagag ggtgggtgaa gcagttgcaa atagattaca
     2761 aaggcaacga aataatgacg gcagctgcct ctcaagggct gacccgtaaa ggtgtgtatg
     2821 ccgttcggta caaggtgaat gaaaatcctc tgtacgcacc cacctcagaa catgtgaacg
     2881 tcctactgac ccgcacggag gaccgcatcg tgtggaaaac actagccggc gacccatgga
     2941 taaaaacact gactgccaag taccctggga atttcactgc cacgatagag gagtggcaag
     3001 cagagcatga tgccatcatg aggcacatct tggagagacc ggaccctacc gacgtcttcc
     3061 agaataaggc aaacgtgtgt tgggccaagg ctttagtgcc ggtgctgaag accgctggca
     3121 tagacatgac cactgaacaa tggaacactg tggattattt tgaaacggac aaagctcact
     3181 cagcagagat agtattgaac caactatgcg tgaggttctt tggactcgat ctggactccg
     3241 gtctattttc tgcacccact gttccgttat ccattaggaa taatcactgg gataactccc
     3301 cgtcgcctaa catgtacggg ctgaataaag aagtggtccg tcagctctct cgcaggtacc
     3361 cacaactgcc tcgggcagtt gccactggaa gagtctatga catgaacact ggtacactgc
     3421 gcaattatga tccgcgcata aacctagtac ctgtaaacag aagactgcct catgctttag
     3481 tcctccacca taatgaacac ccacagagtg acttttcttc attcgtcagc aaattgaagg
     3541 gcagaactgt cctggtggtc ggggaaaagt tgtccgtccc aggcaaaatg gttgactggt
     3601 tgtcagaccg gcctgaggct accttcagag ctcggctgga tttaggcatc ccaggtgatg
     3661 tgcccaaata tgacataata tttgttaatg tgaggacccc atataaatac catcactatc
     3721 agcagtgtga agaccatgcc attaagctta gcatgttgac caagaaagct tgtctgcatc
     3781 tgaatcccgg cggaacctgt gtcagcatag gttatggtta cgctgacagg gccagcgaaa
     3841 gcatcattgg tgctatagcg cggcagttca agttttcccg ggtatgcaaa ccgaaatcct
     3901 cacttgaaga gacggaagtt ctgtttgtat tcattgggta cgatcgcaag gcccgtacgc
     3961 acaattctta caagctttca tcaaccttga ccaacattta tacaggttcc agactccacg
     4021 aagccggatg tgcaccctca tatcatgtgg tgcgagggga tattgccacg gccaccgaag
     4081 gagtgattat aaatgctgct aacagcaaag gacaacctgg cggaggggtg tgcggagcgc
     4141 tgtataagaa attcccggaa agcttcgatt tacagccgat cgaagtagga aaagcgcgac
     4201 tggtcaaagg tgcagctaaa catatcattc atgccgtagg accaaacttc aacaaagttt
     4261 cggaggttga aggtgacaaa cagttggcag aggcttatga gtccatcgct aagattgtca
     4321 acgataacaa ttacaagtca gtagcgattc cactgttgtc caccggcatc ttttccggga
     4381 acaaagatcg actaacccaa tcattgaacc atttgctgac agctttagac accactgatg
     4441 cagatgtagc catatactgc agggacaaga aatgggaaat gactctcaag gaagcagtgg
     4501 ctaggagaga agcagtggag gagatatgca tatccgacga ctcttcagtg acagaacctg
     4561 atgcagagct ggtgagggtg catccgaaga gttctttggc tggaaggaag ggctacagca
     4621 caagcgatgg caaaactttc tcatatttgg aagggaccaa gtttcaccag gcggccaagg
     4681 atatagcaga aattaatgcc atgtggcccg ttgcaacgga ggccaatgag caggtatgca
     4741 tgtatatcct cggagaaagc atgagcagta ttaggtcgaa atgccccgtc gaagagtcgg
     4801 aagcctccac accacctagc acgctgcctt gcttgtgcat ccatgccatg actccagaaa
     4861 gagtacagcg cctaaaagcc tcacgtccag aacaaattac tgtgtgctca tcctttccat
     4921 tgccgaagta tagaatcact ggtgtgcaga agatccaatg ctcccagcct atattgttct
     4981 caccgaaagt gcctgcgtat attcatccaa ggaagtatct cgtggaaaca ccaccggtag
     5041 acgagactcc ggagccatcg gcagagaacc aatccacaga ggggacacct gaacaaccac
     5101 cacttataac cgaggatgag accaggacta gaacgcctga gccgatcatc atcgaagagg
     5161 aagaagagga tagcataagt ttgctgtcag atggcccgac ccaccaggtg ctgcaagtcg
     5221 aggcagacat tcacgggccg ccctctgtat ctagctcatc ctggtccatt cctcatgcat
     5281 ccgactttga tgtggacagt ttatccatac ttgacaccct ggagggagct agcgtgacca
     5341 gcggggcaac gtcagccgag actaactctt acttcgcaaa gagtatggag tttctggcgc
     5401 gaccggtgcc tgcgcctcga acagtattca ggaaccctcc acatcccgct ccgcgcacaa
     5461 gaacaccgtc acttgcaccc agcagggcct gctcgagaac cagcctagtt tccaccccgc
     5521 caggcgtgaa tagggtgatc actagagagg agctcgaggc gcttaccccg tcacgcactc
     5581 ctagcaggtc ggtctcgaga accagcctgg tctccaaccc gccaggcgta aatagggtga
     5641 ttacaagaga ggagtttgag gcgttcgtag cacaacaaca atgacggttt gatgcgggtg
     5701 catacatctt ttcctccgac accggtcaag ggcatttaca acaaaaatca gtaaggcaaa
     5761 cggtgctatc cgaagtggtg ttggagagga ccgaattgga gatttcgtat gccccgcgcc
     5821 tcgaccaaga aaaagaagaa ttactacgca agaaattaca gttaaatccc acacctgcta
     5881 acagaagcag ataccagtcc aggaaggtgg agaacatgaa agccataaca gctagacgta
     5941 ttctgcaagg cctagggcat tatttgaagg cagaaggaaa agtggagtgc taccgaaccc
     6001 tgcatcctgt tcctttgtat tcatctagtg tgaaccgtgc cttttcaagc cccaaggtcg
     6061 cagtggaagc ctgtaacgcc atgttgaaag agaactttcc gactgtggct tcttactgta
     6121 ttattccaga gtacgatgcc tatttggaca tggttgacgg agcttcatgc tgcttagaca
     6181 ctgccagttt ttgccctgca aagctgcgca gctttccaaa gaaacactcc tatttggaac
     6241 ccacaatacg atcggcagtg ccttcagcga tccagaacac gctccagaac gtcctggcag
     6301 ctgccacaaa aagaaattgc aatgtcacgc aaatgagaga attgcccgta ttggattcgg
     6361 cggcctttaa tgtggaatgc ttcaagaaat atgcgtgtaa taatgaatat tgggaaacgt
     6421 ttaaagaaaa ccccatcagg cttactgaag aaaacgtggt aaattacatt accaaattaa
     6481 aaggaccaaa agctgctgct ctttttgcga agacacataa tttgaatatg ttgcaggaca
     6541 taccaatgga caggtttgta atggacttaa agagagacgt gaaagtgact ccaggaacaa
     6601 aacatactga agaacggccc aaggtacagg tgatccaggc tgccgatccg ctagcaacag
     6661 cgtatctgtg cggaatccac cgagagctgg ttaggagatt aaatgcggtc ctgcttccga
     6721 acattcatac actgtttgat atgtcggctg aagactttga cgctattata gccgagcact
     6781 tccagcctgg ggattgtgtt ctggaaactg acatcgcgtc gtttgataaa agtgaggacg
     6841 acgccatggc tctgaccgcg ttaatgattc tggaagactt aggtgtggac gcagagctgt
     6901 tgacgctgat tgaggcggct ttcggcgaaa tttcatcaat acatttgccc actaaaacta
     6961 aatttaaatt cggagccatg atgaaatctg gaatgttcct cacactgttt gtgaacacag
     7021 tcattaacat tgtaatcgca agcagagtgt tgagagaacg gctaaccgga tcaccatgtg
     7081 cagcattcat tggagatgac aatatcgtga aaggagtcaa atcggacaaa ttaatggcag
     7141 acaggtgcgc cacctggttg aatatggaag tcaagattat agatgctgtg gtgggcgaga
     7201 aagcgcctta tttctgtgga gggtttattt tgtgtgactc cgtgaccggc acagcgtgcc
     7261 gtgtggcaga ccccctaaaa aggctgttta agcttggcaa acctctggca gcagacgatg
     7321 aacatgatga tgacaggaga agggcattgc atgaagagtc aacacgctgg aaccgagtgg
     7381 gtattctttc agagctgtgc aaggcagtag aatcaaggta tgaaaccgta ggaacttcca
     7441 tcatagttat ggccatgact actctagcta gcagtgttaa atcattcagc tacctgagag
     7501 gggcccctat aactctctac ggctaacctg aatggactac gacatagtct agtccgccaa
     7561 gtctagcata tgggcgcgtg aattcgccac catggcggga cacctggctt cggatttcgc
     7621 cttctcgccc cctccaggtg gtggaggtga tgggccaggg gggccggagc cgggctgggt
     7681 tgatcctcgg acctggctaa gcttccaagg ccctcctgga gggccaggaa tcgggccggg
     7741 ggttgggcca ggctctgagg tgtgggggat tcccccatgc cccccgccgt atgagttctg
     7801 tggggggatg gcgtactgtg ggccccaggt tggagtgggg ctagtgcccc aaggcggctt
     7861 ggagacctct cagcctgagg gcgaagcagg agtcggggtg gagagcaact ccgatggggc
     7921 ctccccggag ccctgcaccg tcacccctgg tgccgtgaag ctggagaagg agaagctgga
     7981 gcaaaacccg gaggagtccc aggacatcaa agctctgcag aaagaactcg agcaatttgc
     8041 caagctcctg aagcagaaga ggatcaccct gggatataca caggccgatg tggggctcac
     8101 cctgggggtt ctatttggga aggtattcag ccaaacgacc atctgccgct ttgaggctct
     8161 gcagcttagc ttcaagaaca tgtgtaagct gcggcccttg ctgcagaagt gggtggagga
     8221 agctgacaac aatgaaaatc ttcaggagat atgcaaagca gaaaccctcg tgcaggcccg
     8281 aaagagaaag cgaaccagta tcgagaaccg agtgagaggc aacctggaga atttgttcct
     8341 gcagtgcccg aaacccacac tgcagcagat cagccacatc gcccagcagc ttgggctcga
     8401 gaaggatgtg gtccgagtgt ggttctgtaa ccggcgccag aagggcaagc gatcaagcag
     8461 cgactatgca caacgagagg attttgaggc tgctgggtct cctttctcag ggggaccagt
     8521 gtcctttcct ctggccccag ggccccattt tggtacccca ggctatggga gccctcactt
     8581 cactgcactg tactcctcgg tccctttccc tgagggggaa gcctttcccc ctgtctccgt
     8641 caccactctg ggctctccca tgcattcaaa ctctagtgag ggcagaggaa gtctgctaac
     8701 atgcggtgac gtcgaggaga atcctggccc acaattgatg gctgtcagcg acgcgctgct
     8761 cccatctttc tccacgttcg cgtctggccc ggcgggaagg gagaagacac tgcgtcaagc
     8821 aggtgccccg aataaccgct ggcgggagga gctctcccac atgaagcgac ttcccccagt
     8881 gcttcccggc cgcccctatg acctggcggc ggcgaccgtg gccacagacc tggagagcgg
     8941 cggagccggt gcggcttgcg gcggtagcaa cctggcgccc ctacctcgga gagagaccga
     9001 ggagttcaac gatctcctgg acctggactt tattctctcc aattcgctga cccatcctcc
     9061 ggagtcagtg gccgccaccg tgtcctcgtc agcgtcagcc tcctcttcgt cgtcgccgtc
     9121 gagcagcggc cctgccagcg cgccctccac ctgcagcttc acctatccga tccgggccgg
     9181 gaacgacccg ggcgtggcgc cgggcggcac gggcggaggc ctcctctatg gcagggagtc
     9241 cgctccccct ccgacggctc ccttcaacct ggcggacatc aacgacgtga gcccctcggg
     9301 cggcttcgtg gccgagctcc tgcggccaga attggacccg gtgtacattc cgccgcagca
     9361 gccgcagccg ccaggtggcg ggctgatggg caagttcgtg ctgaaggcgt cgctgagcgc
     9421 ccctggcagc gagtacggca gcccgtcggt catcagcgtc agcaaaggca gccctgacgg
     9481 cagccacccg gtggtggtgg cgccctacaa cggcgggccg ccgcgcacgt gccccaagat
     9541 caagcaggag gcggtctctt cgtgcaccca cttgggcgct ggaccccctc tcagcaatgg
     9601 ccaccggccg gctgcacacg acttccccct ggggcggcag ctccccagca ggactacccc
     9661 gaccctgggt cttgaggaag tgctgagcag cagggactgt caccctgccc tgccgcttcc
     9721 tcccggcttc catccccacc cggggcccaa ttacccatcc ttcctgcccg atcagatgca
     9781 gccgcaagtc ccgccgctcc attaccaaga gctcatgcca cccggttcct gcatgccaga
     9841 ggagcccaag ccaaagaggg gaagacgatc gtggccccgg aaaaggaccg ccacccacac
     9901 ttgtgattac gcgggctgcg gcaaaaccta cacaaagagt tcccatctca aggcacacct
     9961 gcgaacccac acaggtgaga aaccttacca ctgtgactgg gacggctgtg gatggaaatt
    10021 cgcccgctca gatgaactga ccaggcacta ccgtaaacac acggggcacc gcccgttcca
    10081 gtgccaaaaa tgcgaccgag cattttccag gtcggaccac ctcgccttac acatgaagag
    10141 gcatttttct agacaatgta ctaactacgc tttgttgaaa ctcgctggcg atgttgaaag
    10201 taaccccggt cctggcgcgc ccatgtacaa catgatggag acggagctga agccgccggg
    10261 cccgcagcaa acttcggggg gcggcggcgg caactccacc gcggcggcgg ccggcggcaa
    10321 ccagaaaaac agcccggacc gcgtcaagcg gcccatgaat gccttcatgg tgtggtcccg
    10381 cgggcagcgg cgcaagatgg cccaggagaa ccccaagatg cacaactcgg agatcagcaa
    10441 gcgcctgggc gccgagtgga aacttttgtc ggagacggag aagcggccgt tcatcgacga
    10501 ggctaagcgg ctgcgagcgc tgcacatgaa ggagcacccg gattataaat accggccccg
    10561 gcggaaaacc aagacgctca tgaagaagga taagtacacg ctgcccggcg ggctgctggc
    10621 ccccggcggc aatagcatgg cgagcggggt cggggtgggc gccggcctgg gcgcgggcgt
    10681 gaaccagcgc atggacagtt acgcgcacat gaacggctgg agcaacggca gctacagcat
    10741 gatgcaggac cagctgggct acccgcagca cccgggcctc aatgcgcacg gcgcagcgca
    10801 gatgcagccc atgcaccgct acgacgtgag cgccctgcag tacaactcca tgaccagctc
    10861 gcagacctac atgaacggct cgcccaccta cagcatgtcc tactcgcagc agggcacccc
    10921 tggcatggct cttggctcca tgggttcggt ggtcaagtcc gaggccagct ccagcccccc
    10981 tgtggttacc tcttcctccc actccagggc gccctgccag gccggggacc tccgggacat
    11041 gatcagcatg tatctccccg gcgccgaggt gccggaaccc gccgccccca gcagacttca
    11101 catgtcccag cactaccaga gcggcccggt gcccggcacg gccattaacg gcacactgcc
    11161 cctctcacac atgtgagcgg ccatcgatgt cgacaactaa cttaagctag caacggtttc
    11221 cctctagcgg gatcaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa
    11281 taaggccggt gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat
    11341 gtgagggccc ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct
    11401 ctcgccaaag gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct
    11461 tcttgaagac aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc
    11521 gacaggtgcc tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa
    11581 ccccagtgcc acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc
    11641 gtattcaaca aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg
    11701 gggcctcggt gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc
    11761 ccgaaccacg gggacgtggt tttcctttga aaaacacgat aataccaatt cgccaccatg
    11821 ggccccctca acgttagctt caccaacagg aactatgacc tcgactacga ctcggtgcag
    11881 ccgtatttct actgcgacga ggaggagaac ttctaccagc agcagcagca gagcgagctg
    11941 cagcccccgg cgcccagcga ggatatctgg aagaaattcg agctgctgcc caccccgccc
    12001 ctgtccccta gccgccgctc cgggctctgc tcgccctcct acgttgcggt cacacccttc
    12061 tcccttcggg gagacaacga cggcggtggc gggagcttct ccacggccga ccagctggag
    12121 atggtgaccg agctgctggg aggagacatg gtgaaccaga gtttcatctg cgacccggac
    12181 gacgagacct tcatcaaaaa catcatcatc caggactgta tgtggagcgg cttctcggcc
    12241 gccgccaagc tcgtctcaga gaagctggcc tcctaccagg ctgcgcgcaa agacagcggc
    12301 agcccgaacc ccgcccgcgg ccacagcgtc tgctccacct ccagcttgta cctgcaggat
    12361 ctgagcgccg ccgcctcaga gtgcatcgac ccctcggtgg tcttccccta ccctctcaac
    12421 gacagcagct cgcccaagtc ctgcgcctcg caagactcca gcgccttctc tccgtcctcg
    12481 gattctctgc tctcctcgac ggagtcctcc ccgcagggca gccccgagcc cctggtgctc
    12541 catgaggaga caccgcccac caccagcagc gactctgagg aggaacaaga agatgaggaa
    12601 gaaatcgatg ttgtttctgt ggaaaagagg caggctcctg gcaaaaggtc agagtctgga
    12661 tcaccttctg ctggaggcca cagcaaacct cctcacagcc cactggtcct caagaggtgc
    12721 cacgtctcca cacatcagca caactacgca gcgcctccct ccactcggaa ggactatcct
    12781 gctgccaaga gggtcaagtt ggacagtgtc agagtcctga gacagatcag caacaaccga
    12841 aaatgcacca gccccaggtc ctcggacacc gaggagaatg tcaagaggcg aacacacaac
    12901 gtcttggagc gccagaggag gaacgagcta aaacggagct tttttgccct gcgtgaccag
    12961 atcccggagt tggaaaacaa tgaaaaggcc cccaaggtag ttatccttaa aaaagccaca
    13021 gcatacatcc tgtccgtcca agcagaggag caaaagctca tttctgaaga ggacttgttg
    13081 cggaaacgac gagaacagtt gaaacacaaa cttgaacagc tacggaactc ttgtgcgtaa
    13141 tctagagtcg acccgggcgg ccgcaactaa cttaagctag caacggtttc cctctagcgg
    13201 gatcaattcc gccccccccc cctaacgtta ctggccgaag ccgcttggaa taaggccggt
    13261 gtgcgtttgt ctatatgtta ttttccacca tattgccgtc ttttggcaat gtgagggccc
    13321 ggaaacctgg ccctgtcttc ttgacgagca ttcctagggg tctttcccct ctcgccaaag
    13381 gaatgcaagg tctgttgaat gtcgtgaagg aagcagttcc tctggaagct tcttgaagac
    13441 aaacaacgtc tgtagcgacc ctttgcaggc agcggaaccc cccacctggc gacaggtgcc
    13501 tctgcggcca aaagccacgt gtataagata cacctgcaaa ggcggcacaa ccccagtgcc
    13561 acgttgtgag ttggatagtt gtggaaagag tcaaatggct ctcctcaagc gtattcaaca
    13621 aggggctgaa ggatgcccag aaggtacccc attgtatggg atctgatctg gggcctcggt
    13681 gcacatgctt tacatgtgtt tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg
    13741 gggacgtggt tttcctttga aaaacacgat aataccatga ccgagtacaa gcccacggtg
    13801 cgcctcgcca cccgcgacga cgtccccagg gccgtacgca ccctcgccgc cgcgttcgcc
    13861 gactaccccg ccacgcgcca caccgtcgat ccggaccgcc acatcgagcg ggtcaccgag
    13921 ctgcaagaac tcttcctcac gcgcgtcggg ctcgacatcg gcaaggtgtg ggtcgcggac
    13981 gacggcgccg cggtggcggt ctggaccacg ccggagagcg tcgaagcggg ggcggtgttc
    14041 gccgagatcg gcccgcgcat ggccgagttg agcggttccc ggctggccgc gcagcaacag
    14101 atggaaggcc tcctggcgcc gcaccggccc aaggagcccg cgtggttcct ggccaccgtc
    14161 ggcgtctcgc ccgaccacca gggcaagggt ctgggcagcg ccgtcgtgct ccccggagtg
    14221 gaggcggccg agcgcgccgg ggtgcccgcc ttcctggaga cctccgcgcc ccgcaacctc
    14281 cccttctacg agcggctcgg cttcaccgtc accgccgacg tcgaggtgcc cgaaggaccg
    14341 cgcacctggt gcatgacccg caagcccggt gcctgagaat tggcaagctg cttacataga
    14401 actcgcggcg attggcatgc cgccttaaaa tttttatttt atttttcttt tcttttccga
    14461 atcggatttt gtttttaata tttcaaaaaa aaaaaaaaaa aaaaaaaaaa cgcgtcgagg
    14521 ggaattaatt cttgaagacg aaagggccag gtggcacttt tcggggaaat gtgcgcggaa
    14581 cccctatttg tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac
    14641 cctgataaat gcttcaataa tattgaaaaa ggaagagtat gagtattcaa catttccgtg
    14701 tcgcccttat tccctttttt gcggcatttt gccttcctgt ttttgctcac ccagaaacgc
    14761 tggtgaaagt aaaagatgct gaagatcagt tgggtgcacg agtgggttac atcgaactgg
    14821 atctcaacag cggtaagatc cttgagagtt ttcgccccga agaacgtttt ccaatgatga
    14881 gcacttttaa agttctgcta tgtggcgcgg tattatcccg tgttgacgcc gggcaagagc
    14941 aactcggtcg ccgcatacac tattctcaga atgacttggt tgagtactca ccagtcacag
    15001 aaaagcatct tacggatggc atgacagtaa gagaattatg cagtgctgcc ataaccatga
    15061 gtgataacac tgcggccaac ttacttctga caacgatcgg aggaccgaag gagctaaccg
    15121 cttttttgca caacatgggg gatcatgtaa ctcgccttga tcgttgggaa ccggagctga
    15181 atgaagccat accaaacgac gagcgtgaca ccacgatgcc tgtagcaatg gcaacaacgt
    15241 tgcgcaaact attaactggc gaactactta ctctagcttc ccggcaacaa ttaatagact
    15301 ggatggaggc ggataaagtt gcaggaccac ttctgcgctc ggcccttccg gctggctggt
    15361 ttattgctga taaatctgga gccggtgagc gtgggtctcg cggtatcatt gcagcactgg
    15421 ggccagatgg taagccctcc cgtatcgtag ttatctacac gacggggagt caggcaacta
    15481 tggatgaacg aaatagacag atcgctgaga taggtgcctc actgattaag cattggtaac
    15541 tgtcagacca agtttactca tatatacttt agattgattt aaaacttcat ttttaattta
    15601 aaaggatcta ggtgaagatc ctttttgata atctcatgac caaaatccct taacgtgagt
    15661 tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa aggatcttct tgagatcctt
    15721 tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc accgctacca gcggtggttt
    15781 gtttgccgga tcaagagcta ccaactcttt ttccgaaggt aactggcttc agcagagcgc
    15841 agataccaaa tactgtcctt ctagtgtagc cgtagttagg ccaccacttc aagaactctg
    15901 tagcaccgcc tacatacctc gctctgctaa tcctgttacc agtggctgct gccagtggcg
    15961 ataagtcgtg tcttaccggg ttggactcaa gacgatagtt accggataag gcgcagcggt
    16021 cgggctgaac ggggggttcg tgcacacagc ccagcttgga gcgaacgacc tacaccgaac
    16081 tgagatacct acagcgtgag cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg
    16141 acaggtatcc ggtaagcggc agggtcggaa caggagagcg cacgagggag cttccagggg
    16201 gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat
    16261 ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa cgccagcaac gcgagctcta
    16321 atacgactca ctatag
//