| 质粒名称: | mito-dendra2 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 55796 |
| 存储实验室: | David Chan |
|
载体骨架基本信息 |
|
| 载体名称: | pCMV |
| 载体出品公司: | Clontech |
| 载体抗性: | 卡那霉素 |
| 空载体大小: | 3920 bp |
| 完整质粒大小: | 4740 bp |
| 载体修饰: | -- |
| 载体类型: | 哺乳细胞表达 |
| 筛选标记: | 新霉素（G418） |
| 高拷贝/低拷贝: | -- |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | mito-Dendra2,cox8a-Dendra2 |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 816 bp |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CMV |
| 标签/融合蛋白: | -- |
| 克隆方法: | 限制性内切酶 |
| 5' 克隆位点: | Nhe1(未破坏) |
| 3' 克隆位点: | Not1 (未破坏) |
| 5' 测序引物 | CMV Forward |
| 3' 测序引物 | SV40pA-R |
|
文献引用方法 |
|
| 材料和方法部分: | mito-dendra2 was a gift from David Chan (Addgene plasmid # 55796 ; http://n2t.net/addgene:55796 ; RRID:Addgene_55796) |
| 参考文献部分: | Mouse lines with photo-activatable mitochondria to study mitochondrial dynamics. Pham AH, McCaffery JM, Chan DC. Genesis. 2012 Nov;50(11):833-43. doi: 10.1002/dvg.22050. Epub 2012 Aug 11. 10.1002/dvg.22050 PubMed 22821887 |
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mito-dendra2载体 mitodendra2质粒基本信息
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mito-dendra2质粒 mitodendra2载体简介
mito-dendra2质粒序列 mitodendra2载体序列
LOCUS mito-dendra2 4741 bp ds-DNA circular SYN 19-MAR-2019
DEFINITION photo-activatable mitochondrial marker for expression in mammalian
cells.
ACCESSION .
VERSION .
KEYWORDS mito-dendra2
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 4741)
AUTHORS Pham AH, McCaffery JM, Chan DC
TITLE Mouse lines with photo-activatable mitochondria to study
mitochondrial dynamics.
JOURNAL Genesis. 2012 Nov;50(11):833-43. doi: 10.1002/dvg.22050. Epub 2012
Aug 11.
PUBMED 22821887
REFERENCE 2 (bases 1 to 4741)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Mar 19, 2019 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..4741
/organism="synthetic DNA construct"
/mol_type="other DNA"
enhancer 61..364
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 365..568
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 519..539
/label=CMV-F
/note="Human CMV immediate early promoter, forward primer"
CDS 693..1379
/codon_start=1
/product="monomeric photoswitchable green-to-red
fluorescent protein from Dendronephthya"
/label=Dendra2
/note="mammalian codon-optimized"
/translation="NTPGINLIKEDMRVKVHMEGNVNGHAFVIEGEGKGKPYEGTQTAN
LTVKEGAPLPFSYDILTTAVHYGNRVFTKYPEDIPDYFKQSFPEGYSWERTMTFEDKGI
CTIRSDISLEGDCFFQNVRFKGTNFPPNGPVMQKKTLKWEPSTEKLHVRDGLLVGNINM
ALLLEGGGHYLCDFKTTYKAKKVVQLPDAHFVDHRIEILGNDSDYNKVKLYEHAVARYS
PLPSQVW"
polyA_signal 1529..1650
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(1566..1585)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 1620..1639
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
rep_origin complement(1657..2112)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(1794..1815)
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind 2006..2025
/label=F1ori-R
/note="F1 origin, reverse primer"
promoter 2139..2243
/gene="bla"
/label=AmpR promoter
promoter 2245..2602
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
primer_bind complement(2268..2288)
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
rep_origin 2453..2588
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind 2515..2534
/label=SV40pro-F
/note="SV40 promoter/origin, forward primer"
CDS 2637..3431
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKASMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(2691..2710)
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
primer_bind 3301..3320
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind complement(3619..3638)
/label=TK-pA-R
/note="Thymidine kinase polyA, reverse primer"
polyA_signal 3663..3710
/label=HSV TK poly(A) signal
/note="herpes simplex virus thymidine kinase
polyadenylation signal (Cole and Stacy, 1985)"
rep_origin 4039..4627
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 4528..4547
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
ORIGIN
1 tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
61 cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
121 gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
181 atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
241 aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
301 catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
361 catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
421 atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
481 ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
541 acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcatgt
601 ccgtcctgac gccgctgctg ctgcggggct tgacaggctc ggcccggcgg ctcccagtgc
661 cgcgcgccaa gatccattcg ttgggggatc cgaacacccc gggaattaac ctgatcaagg
721 aggacatgcg cgtgaaggtg cacatggagg gcaacgtgaa cggccacgcc ttcgtgatcg
781 agggcgaggg caagggcaag ccctacgagg gcacccagac cgccaacctg accgtgaagg
841 agggcgcccc cctgcccttc agctacgaca tcctgaccac cgccgtgcac tacggcaacc
901 gggtgttcac caagtacccc gaggacatcc ccgactactt caagcagagc ttccccgagg
961 gctacagctg ggagcgcacc atgaccttcg aggacaaggg catctgcacc atccgcagcg
1021 acatcagcct ggagggcgac tgcttcttcc agaacgtgcg cttcaagggc accaacttcc
1081 cccccaacgg ccccgtgatg cagaagaaga ccctgaagtg ggagcccagc accgagaagc
1141 tgcacgtgcg cgacggcctg ctggtgggca acatcaacat ggccctgctg ctggagggcg
1201 gcggccacta cctgtgcgac ttcaagacca cctacaaggc caagaaggtg gtgcagctgc
1261 ccgacgccca cttcgtggac caccgcatcg agatcctggg caacgacagc gactacaaca
1321 aggtgaagct gtacgagcac gccgtggccc gctacagccc cctgcccagc caggtgtggt
1381 ccggcgacag cggcgtgtac aagtaaagcg gccgcgactc tagatcataa tcagccatac
1441 cacatttgta gaggttttac ttgctttaaa aaacctccca cacctccccc tgaacctgaa
1501 acataaaatg aatgcaattg ttgttgttaa cttgtttatt gcagcttata atggttacaa
1561 ataaagcaat agcatcacaa atttcacaaa taaagcattt ttttcactgc attctagttg
1621 tggtttgtcc aaactcatca atgtatctta aggcgtaaat tgtaagcgtt aatattttgt
1681 taaaattcgc gttaaatttt tgttaaatca gctcattttt taaccaatag gccgaaatcg
1741 gcaaaatccc ttataaatca aaagaataga ccgagatagg gttgagtgtt gttccagttt
1801 ggaacaagag tccactatta aagaacgtgg actccaacgt caaagggcga aaaaccgtct
1861 atcagggcga tggcccacta cgtgaaccat caccctaatc aagttttttg gggtcgaggt
1921 gccgtaaagc actaaatcgg aaccctaaag ggagcccccg atttagagct tgacggggaa
1981 agccggcgaa cgtggcgaga aaggaaggga agaaagcgaa aggagcgggc gctagggcgc
2041 tggcaagtgt agcggtcacg ctgcgcgtaa ccaccacacc cgccgcgctt aatgcgccgc
2101 tacagggcgc gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
2161 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
2221 aataatattg aaaaaggaag agtcctgagg cggaaagaac cagctgtgga atgtgtgtca
2281 gttagggtgt ggaaagtccc caggctcccc agcaggcaga agtatgcaaa gcatgcatct
2341 caattagtca gcaaccaggt gtggaaagtc cccaggctcc ccagcaggca gaagtatgca
2401 aagcatgcat ctcaattagt cagcaaccat agtcccgccc ctaactccgc ccatcccgcc
2461 cctaactccg cccagttccg cccattctcc gccccatggc tgactaattt tttttattta
2521 tgcagaggcc gaggccgcct cggcctctga gctattccag aagtagtgag gaggcttttt
2581 tggaggccta ggcttttgca aagatcgatc aagagacagg atgaggatcg tttcgcatga
2641 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
2701 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
2761 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcaag
2821 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
2881 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
2941 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
3001 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
3061 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
3121 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgagcatg cccgacggcg
3181 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
3241 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
3301 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
3361 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
3421 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
3481 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
3541 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
3601 ccctaggggg aggctaactg aaacacggaa ggagacaata ccggaaggaa cccgcgctat
3661 gacggcaata aaaagacaga ataaaacgca cggtgttggg tcgtttgttc ataaacgcgg
3721 ggttcggtcc cagggctggc actctgtcga taccccaccg agaccccatt ggggccaata
3781 cgcccgcgtt tcttcctttt ccccacccca ccccccaagt tcgggtgaag gcccagggct
3841 cgcagccaac gtcggggcgg caggccctgc catagcctca ggttactcat atatacttta
3901 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
3961 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
4021 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
4081 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
4141 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
4201 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
4261 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
4321 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
4381 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
4441 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
4501 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
4561 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
4621 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
4681 tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgccatgca
4741 t
//
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