pLVX-TetOn-C3-EGFP

LOCUS       pLVX-TetOn-C3-EGFP               10743 bp DNA     circular SYN 02-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 10743)
  AUTHORS   Trial User
  TITLE     Direct Submission
  JOURNAL   Exported Aug 4, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..10743
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     polyA_signal    216..350
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     promoter        398..502
                     /gene="bla"
                     /label=AmpR promoter
     CDS             503..1363
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      1534..2119
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     protein_bind    2407..2428
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     promoter        2443..2473
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    2481..2497
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     primer_bind     2505..2521
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     LTR             2650..3283
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
     misc_feature    3491..4079
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             3597..3608
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     CDS             complement(4093..4692)
                     /codon_start=1
                     /gene="pac from Streptomyces alboniger"
                     /product="puromycin N-acetyltransferase"
                     /label=PuroR
                     /note="confers resistance to puromycin"
                     /translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
                     VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
                     AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
                     APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
     promoter        complement(4701..5030)
                     /label=SV40 promoter
                     /note="SV40 enhancer and early promoter"
     rep_origin      4715..4850
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     CDS             complement(5041..5787)
                     /codon_start=1
                     /product="modified rtTA protein that binds tightly to 
                     promoters containing the tet operator in the presence of 
                     doxycycline"
                     /label=Tet-On(R) 3G
                     /translation="MSRLDKSKVINSALELLNGVGIEGLTTRKLAQKLGVEQPTLYWHV
                     KNKRALLDALPIEMLDRHHTHSCPLEGESWQDFLRNNAKSYRCALLSHRDGAKVHLGTR
                     PTEKQYETLENQLAFLCQQGFSLENALYALSAVGHFTLGCVLEEQEHQVAKEERETPTT
                     DSMPPLLKQAIELFDRQGAEPAFLFGLELIICGLEKQLKCESGGPTDALDDFDLDMLPA
                     DALDDFDLDMLPADALDDFDLDMLPG"
     promoter        complement(5806..6316)
                     /label=hPGK promoter
                     /note="human phosphoglycerate kinase 1 promoter"
     promoter        6333..6700
                     /label=TRE3GS promoter
                     /note="3rd-generation Tet-responsive promoter that can be 
                     activated by binding of Tet-On(R) 3G, modified to eliminate
                     binding sites for endogenous mammalian transcription 
                     factors"
     protein_bind    6341..6359
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6377..6395
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6413..6431
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6449..6467
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6485..6503
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6521..6539
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     protein_bind    6557..6575
                     /gene="tetO"
                     /label=tet operator
                     /bound_moiety="tetracycline repressor TetR"
                     /note="bacterial operator O2 for the tetR and tetA genes"
     primer_bind     6691..6735
                     /label=C3-plvx F
     misc_feature    6707..7462
                     /label=limosum C3
     primer_bind     complement(7443..7477)
                     /label=C3 plvx R
     CDS             7469..7525
                     /codon_start=1
                     /product="2A peptide from porcine teschovirus-1 
                     polyprotein"
                     /label=P2A
                     /note="Eukaryotic ribosomes fail to insert a peptide bond 
                     between the Gly and Pro residues, yielding separate 
                     polypeptides."
                     /translation="ATNFSLLKQAGDVEENPGP"
     CDS             7526..8242
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     polyA_signal    8423..8557
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     misc_feature    8601..8716
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1 (lacking the first T)"
     CDS             complement(8979..9023)
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    9208..9441
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    9938..10063
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     LTR             10110..10743
                     /label=3' LTR
                     /note="3' long terminal repeat (LTR) from HIV-1"
ORIGIN
        1 actactaagt ttgtagtaca tatttaacaa atacaatttc tttaaaaact actaagtttg
       61 tagtacatat ttaacaaata caatttcttt aaaatgaaaa taattcagag gaatcacagg
      121 tttagagtaa atgaaaccac taggtaattg gcagtggtaa tagggtatgg ggtgggaagt
      181 ttgggatgat tttggttagc ttgagttatc cagttgatcc agacatgata agatacattg
      241 atgagtttgg acaaaccaca actagaatgc agtgaaaaaa atgctttatt tgtgaaattt
      301 gtgatgctat tgctttattt gtaaccatta taagctgcaa taaacaagtt gatctcccga
      361 tccgtcgacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta tttgtttatt
      421 tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat aaatgcttca
      481 ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc ttattccctt
      541 ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga aagtaaaaga
      601 tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca acagcggtaa
      661 gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt ttaaagttct
      721 gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg gtcgccgcat
      781 acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc atcttacgga
      841 tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata acactgcggc
      901 caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt tgcacaacat
      961 gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag ccataccaaa
     1021 cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca aactattaac
     1081 tggcgaacta cttactctag cttcccggca acaattaata gactggatgg aggcggataa
     1141 agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg ctgataaatc
     1201 tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag atggtaagcc
     1261 ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg aacgaaatag
     1321 acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag accaagttta
     1381 ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa
     1441 gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc
     1501 gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat
     1561 ctgctgcttg caaacaaaaa aaccgctacc agcggtggtt tgtttgccgg atcaagagct
     1621 accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgttct
     1681 tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     1741 cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     1801 gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     1861 gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     1921 gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     1981 cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     2041 tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     2101 ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     2161 ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     2221 taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     2281 agtgagcgag gaagcggaag agcgcccaat acgcaaaccg cctctccccg cgcgttggcc
     2341 gattcattaa tgcagctggc acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa
     2401 cgcaattaat gtgagttagc tcactcatta ggcaccccag gctttacact ttatgcttcc
     2461 ggctcgtatg ttgtgtggaa ttgtgagcgg ataacaattt cacacaggaa acagctatga
     2521 ccatgattac gccaagctct agctagaggt cgacggtaaa acgctagcaa tgtcaaggcc
     2581 tctcactctc tgatattcat ttctttgcaa gttataaata ctgaataata agatgacatg
     2641 aactactact gctagagatt ttccacactg actaaaaggg tctgagggat ctctagttac
     2701 cagagtcaca caacagacgg gcacacacta cttgaagcac tcaaggcaag ctttattgag
     2761 gcttaagcag tgggttccct agttagccag agagctccca ggctcagatc tggtctaacc
     2821 agagagaccc agtacaggca aaaagcagct gcttatatgc aggatctgag ggctcgccac
     2881 tccccagtcc cgcccaggcc acgcctccct ggaaagtccc cagcggaaag tcccttgtag
     2941 caagctcgat atcagcagtt cttgaagtac tccggatgca gctctcgggc cacgtgatga
     3001 aatgctaggc ggctgtcaaa cctccactct aacacttctc tctccgggtc atccatccca
     3061 tgcaggctca cagggtgtaa caagctggtg ttctctcctt tattggcctc ttctacctta
     3121 tctggctcaa ctggtactag cttgtagcac catccaaagg tcagtggata tctgacccct
     3181 ggccctggtg tgtagttctg ctaatcaggg aagtagcctt gtgtgtggta gatccacaga
     3241 tcaaggatat cttgtcttcg ttgggagtga attagccctt ccagtcccct cttttctttt
     3301 aaaaagtggc taagatctac agctgccttg taagtcattg gtcttaaagg tacctgaggt
     3361 gtgactggaa aaacccacct cctcctcctc ttgtgcttct agccaggcac aatcagcatt
     3421 ggtagctgct gtattgctac ttgtgattgc tccatgtttt tctaggtctc gactgcagaa
     3481 ttaattccag gcggggaggc ggcccaaagg gagatccgac tcgtctgagg gcgaaggcga
     3541 agacgcggaa gaggccgcag agccggcagc aggccgcggg aaggaaggtc cgctggattg
     3601 agggccgaag ggacgtagca gaaggacgtc ccgcgcagaa tccaggtggc aacacaggcg
     3661 agcagccatg gaaaggacgt cagcttcccc gacaacacca cggaattgtc agtgcccaac
     3721 agccgagccc ctgtccagca gcgggcaagg caggcggcga tgagttccgc cgtggcaata
     3781 gggaggggga aagcgaaagt cccggaaagg agctgacagg tggtggcaat gccccaacca
     3841 gtgggggttg cgtcagcaaa cacagtgcac accacgccac gttgcctgac aacgggccac
     3901 aactcctcat aaagagacag caaccaggat ttatacaagg aggagaaaat gaaagccata
     3961 cgggaagcaa tagcatgata caaaggcatt aaagcagcgt atccacatag cgtaaaagga
     4021 gcaacatagt taagaatacc agtcaatctt tcacaaattt tgtaatccag aggttgattg
     4081 ttccagacgc gttcaggcac cgggcttgcg ggtcatgcac caggtgcgcg gtccttcggg
     4141 cacctcgacg tcggcggtga cggtgaagcc gagccgctcg tagaagggga ggttgcgggg
     4201 cgcggaggtc tccaggaagg cgggcacccc ggcgcgctcg gccgcctcca ctccggggag
     4261 cacgacggcg ctgcccagac ccttgccctg gtggtcgggc gagacgccga cggtggccag
     4321 gaaccacgcg ggctccttgg gccggtgcgg cgccaggagg ccttccatct gttgctgcgc
     4381 ggccagccgg gaaccgctca actcggccat gcgcgggccg atctcggcga acaccgcccc
     4441 cgcttcgacg ctctccggcg tggtccagac cgccaccgcg gcgccgtcgt ccgcgaccca
     4501 caccttgccg atgtcgagcc cgacgcgcgt gaggaagagt tcttgcagct cggtgacccg
     4561 ctcgatgtgg cggtccgggt cgacggtgtg gcgcgtggcg gggtagtcgg cgaacgcggc
     4621 ggcgagggtg cgtacggccc gggggacgtc gtcgcgggtg gcgaggcgca ccgtgggctt
     4681 gtactcggtc atggtcgcgt tttgcaaaag cctaggcctc caaaaaagcc tcctcactac
     4741 ttctggaata gctcagaggc cgaggcggcc tcggcctctg cataaataaa aaaaattagt
     4801 cagccatggg gcggagaatg ggcggaactg ggcggagtta ggggcgggat gggcggagtt
     4861 aggggcggga ctatggttgc tgactaattg agatgcatgc tttgcatact tctgcctgct
     4921 ggggagcctg gggactttcc acacctggtt gctgactaat tgagatgcat gctttgcata
     4981 cttctgcctg ctggggagcc tggggacttt ccacacccta actgacacac attcgcgcgt
     5041 ttacccgggg agcatgtcaa ggtcaaaatc gtcaagagcg tcagcaggca gcatatcaag
     5101 gtcaaagtcg tcaagggcat cggctgggag catgtctaag tcaaaatcgt caagggcgtc
     5161 ggtcggcccg ccgctttcgc actttagctg tttctccagg ccacatatga ttagttccag
     5221 gccgaaaagg aaggcaggtt cggctccctg ccggtcgaac agctcaattg cttgtttcag
     5281 aagtgggggc atagaatcgg tggtaggtgt ctctctttcc tcttttgcta cttgatgctc
     5341 ctgttcctcc aatacgcagc ccagtgtaaa gtggcccacg gcggacagag cgtacagtgc
     5401 gttctccagg gagaagcctt gctgacacag gaacgcgagc tgattttcca gggtttcgta
     5461 ctgtttctct gttgggcggg tgccgagatg cactttagcc ccgtcgcgat gtgagaggag
     5521 agcacagcgg tatgacttgg cgttgttccg cagaaagtct tgccatgact cgccttccag
     5581 ggggcaggag tgggtatgat gcctgtccag catctcgatt ggcagggcat cgagcagggc
     5641 ccgcttgttc ttcacgtgcc agtacagggt aggctgctca actcccagct tttgagcgag
     5701 tttccttgtc gtcaggcctt cgataccgac tccattgagt aattccagag cagagtttat
     5761 gactttgctc ttgtccagtc tagacatggt aattcgatga tccccctggg gagagaggtc
     5821 ggtgattcgg tcaacgaggg agccgactgc cgacgtgcgc tccggaggct tgcagaatgc
     5881 ggaacaccgc gcgggcagga acagggccca cactaccgcc ccacaccccg cctcccgcac
     5941 cgccccttcc cggccgctgc tctcggcgcg ccctgctgag cagccgctat tggccacagc
     6001 ccatcgcggt cggcgcgctg ccattgctcc ctggcgctgt ccgtctgcga gggtactagt
     6061 gagacgtgcg gcttccgttt gtcacgtccg gcacgccgcg aaccgcaagg aaccttcccg
     6121 acttaggggc ggagcaggaa gcgtcgccgg ggggcccaca agggtagcgg cgaagatccg
     6181 ggtgacgctg cgaacggacg tgaagaatgt gcgagaccca gggtcggcgc cgctgcgttt
     6241 cccggaacca cgcccagagc agccgcgtcc ctgcgcaaac ccagggctgc cttggaaaag
     6301 gcgcaacccc aaccccgtgg aattatcacc tcgagtttac tccctatcag tgatagagaa
     6361 cgtatgaaga gtttactccc tatcagtgat agagaacgta tgcagacttt actccctatc
     6421 agtgatagag aacgtataag gagtttactc cctatcagtg atagagaacg tatgaccagt
     6481 ttactcccta tcagtgatag agaacgtatc tacagtttac tccctatcag tgatagagaa
     6541 cgtatatcca gtttactccc tatcagtgat agagaacgta taagctttgc ttatgtaaac
     6601 cagggcgcct ataaaagagt gctgattttt tgagtaaact tcaattccac aacacttttg
     6661 tcttatacca actttccgta ccacttccta ccctcgtaaa gaattcatga ataaactaac
     6721 agaaagggta ttatgtgtgg gcgtgagcgg tctgattttg ttcagcgtag ctgcgctggt
     6781 ccagggcacc aagaagtgct atgcgaaccc ggttcgtaac cgcgcggcta gccgtgttaa
     6841 accgtatgcc gactccttca aggagttcac aaatattgac gaggctcgcg cgtggggtga
     6901 taaacaattt gcgaagtata aactgagcag ctctgaaaaa aacgctctga ccatttatac
     6961 ccgtaatgca gcgcgtatca acggcccact ccgcgccaac cagggaaaca ccaatggctt
     7021 accggcagac atccgtaaag aggtggaaca aatcgacaag tcgttcacga agatgcaaac
     7081 cccggaaaac attatcttgt tccgcggtga tgacccgggt tacctgggtc cggatttcga
     7141 gaacacgatc ttgaatcgcg acggcaccat caataaggcg gtttttgagc aggttaaact
     7201 gcgttttaag ggcaaagatc gtaaagagta cggctacatc tccacttcac tggtgaatgg
     7261 tagcgcattt gcgggtcgtc cgattatcac caagttcaaa gttcttgatg gttccaaagc
     7321 cggttacatt gaaccgatta gcacctttaa gggtcagctg gaagtgctgc tgccgagaag
     7381 ctctacgtac acgatcagcg atatgcaaat tgcgcctaac aacaagcaga tcatcatcac
     7441 cgcattgctg aaacgtctcg agaccggtgc caccaacttc agcctgctga agcaggccgg
     7501 cgacgtggag gagaaccccg gccccgtgag caagggcgag gagctgttca ccggggtggt
     7561 gcccatcctg gtcgagctgg acggcgacgt aaacggccac aagttcagcg tgtccggcga
     7621 gggcgagggc gatgccacct acggcaagct gaccctgaag ttcatctgca ccaccggcaa
     7681 gctgcccgtg ccctggccca ccctcgtgac caccctgacc tacggcgtgc agtgcttcag
     7741 ccgctacccc gaccacatga agcagcacga cttcttcaag tccgccatgc ccgaaggcta
     7801 cgtccaggag cgcaccatct tcttcaagga cgacggcaac tacaagaccc gcgccgaggt
     7861 gaagttcgag ggcgacaccc tggtgaaccg catcgagctg aagggcatcg acttcaagga
     7921 ggacggcaac atcctggggc acaagctgga gtacaactac aacagccaca acgtctatat
     7981 catggccgac aagcagaaga acggcatcaa ggtgaacttc aagatccgcc acaacatcga
     8041 ggacggcagc gtgcagctcg ccgaccacta ccagcagaac acccccatcg gcgacggccc
     8101 cgtgctgctg cccgacaacc actacctgag cacccagtcc aagctgagca aagaccccaa
     8161 cgagaagcgc gatcacatgg tcctgctgga gttcgtgacc gccgccggga tcactctcgg
     8221 catggacgag ctgtacaagt aaggatccag accacctccc ctgcgagcta agctggacag
     8281 ccaatgacgg gtaagagagt gacatttttc actaacctaa gacaggaggg ccgtcagagc
     8341 tactgcctaa tccaaagacg ggtaaaagtg ataaaaatgt atcactccaa cctaagacag
     8401 gcgcagcttc cgagggattt gagatccaga catgataaga tacattgatg agtttggaca
     8461 aaccaaaact agaatgcagt gaaaaaaatg ccttatttgt gaaatttgtg atgctattgc
     8521 cttatttgta accattataa gctgcaataa acaagttaag tcgataaact ggatctctgc
     8581 tgtccctgta ataaacccga aaattttgaa tttttgtaat ttgtttttgt agttctttag
     8641 tttgtatgtc tgttgctatt atgtctacta ttctttcccc tgcactgtac cccccaatcc
     8701 ccccttttct tttaaaggcg ataccgtcga gatccgttca ctaatcgaat ggatctgtct
     8761 ctgtctctct ctccaccttc ttcttctatt ccttcgggcc tgtcgggtcc cctcggggtt
     8821 gggaggtggg tctgaaacga taatggtgaa tatccctgcc taactctatt cactatagaa
     8881 agtacagcaa aaactattct taaacctacc aagcctccta ctatcattat gaataatttt
     8941 atataccaca gccaatttgt tatgttaaac caattccaca aacttgccca tttatctaat
     9001 tccaataatt cttgttcatt cttttcttgc tggttttgcg attcttcaat taaggagtgt
     9061 attaagcttg tgtaattgtt aatttctctg tcccactcca tccaggtcgt gtgattccaa
     9121 atctgttcca gagatttatt actccaacta gcattccaag gcacagcagt ggtgcaaatg
     9181 agttttccag agcaacccca aatccccagg agctgttgat cctttaggta tctttccaca
     9241 gccaggattc ttgcctggag ctgcttgatg ccccagactg tgagttgcaa cagatgctgt
     9301 tgcgcctcaa tagccctcag caaattgttc tgctgctgca ctataccaga caataattgt
     9361 ctggcctgta ccgtcagcgt cattgacgct gcgcccatag tgcttcctgc tgctcccaag
     9421 aacccaagga acaaagctcc tattcccact gctctttttt ctctctgcac cactcttctc
     9481 tttgccttgg tgggtgctac tcctaatggt tcaattttta ctactttata tttatataat
     9541 tcacttctcc aattgtccct catatctcct cctccaggtc tgaagatcag cggccggccg
     9601 cttgctgtgc ggtggtctta cttttgtttt gctcttcctc tatcttgtct aaagcttcct
     9661 tggtgtcttt tatctctatc ctttgatgca cacaatagag ggttgctact gtattatata
     9721 atgatctaag ttcttctgat cctgtctgaa gggatggttg tagctgtccc agtatttgtc
     9781 tacagccttc tgatgtttct aacaggccag gattaactgc gaatcgttct agctccctgc
     9841 ttgcccatac tatatgtttt aatttatatt ttttctttcc ccctggcctt aaccgaattt
     9901 tttcccatcg cgatctaatt ctcccccgct taatactgac gctctcgcac ccatctctct
     9961 ccttctagcc tccgctagtc aaaatttttg gcgtactcac cagtcgccgc ccctcgcctc
    10021 ttgccgtgcg cgcttcagca agccgagtcc tgcgtcgaga gagctcctct ggtttccctt
    10081 tcgctttcaa gtccctgttc gggcgccact gctagagatt ttccacactg actaaaaggg
    10141 tctgagggat ctctagttac cagagtcaca caacagacgg gcacacacta cttgaagcac
    10201 tcaaggcaag ctttattgag gcttaagcag tgggttccct agttagccag agagctccca
    10261 ggctcagatc tggtctaacc agagagaccc agtacaggca aaaagcagct gcttatatgc
    10321 aggatctgag ggctcgccac tccccagtcc cgcccaggcc acgcctccct ggaaagtccc
    10381 cagcggaaag tcccttgtag caagctcgat atcagcagtt cttgaagtac tccggatgca
    10441 gctctcgggc cacgtgatga aatgctaggc ggctgtcaaa cctccactct aacacttctc
    10501 tctccgggtc atccatccca tgcaggctca cagggtgtaa caagctggtg ttctctcctt
    10561 tattggcctc ttctacctta tctggctcaa ctggtactag cttgtagcac catccaaagg
    10621 tcagtggata tctgacccct ggccctggtg tgtagttctg ctaatcaggg aagtagcctt
    10681 gtgtgtggta gatccacaga tcaaggatat cttgtcttct ttgggagtga attagccctt
    10741 cca
//