| 质粒名称: | pAC-ZEAXipi |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 53287 |
| 存储实验室: | pAC-EHER |
|
载体骨架基本信息 |
|
| 载体名称: | pAC-EHER |
| 载体出品公司: | Francis X. Cunningham, Jr. |
| 空载体大小: | 13581 bp |
| 完整质粒大小: | 10040 bp |
| 载体类型: | low copy number bacterial cloning vector |
| 载体抗性: | Chloramphenicol |
| 生长温度: | 30 ℃ |
| 宿主菌: | Top10 |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | crtE, idi, crtY, crtI, crtB, crtZ / idi also known as ORF6 |
| 物种来源: | Erwinia herbicola Eho10 |
| GenBank编号 | M87280.1 |
| 启动子: | dogenous promoters |
| 标签/融合蛋白: | 人细胞色素c氧化酶亚基VIII（N端） |
| 克隆方法: | 限制性内切酶 |
| 5’克隆位点: | SphI (destroyed during cloning) |
| 3’克隆位点: | DraIII (destroyed during cloning) |
| 5’测序引物 | none |
| 3’测序引物 | none |
|
文献引用方法 |
|
| 材料和方法部分: | pAC-ZEAXipi was a gift from Francis X Cunningham Jr (Addgene plasmid # 53287 ; http://n2t.net/addgene:53287 ; RRID:Addgene_53287) |
| 参考文献部分: | A portfolio of plasmids for identification and analysis of carotenoid pathway enzymes: Adonis aestivalis as a case study. Cunningham FX Jr, Gantt E. Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17. 10.1007/s11120-007-9210-0 PubMed 17 |
买家导航
pAC-ZEAXipi载体质粒基本信息
pAC-ZEAXipi质粒图谱载体图谱和pAC-ZEAXipi载体序列质粒序列多克隆位点信息
pAC-ZEAXipi质粒载体简介
pAC-ZEAXipi质粒序列载体序列
LOCUS pAC-ZEAXipi 10041 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Contains crtE, idi, crtI, crtY, crtB, and crtZ genes of Erwinia
herbicola (Pantoea agglomerans) Eho10 and thereby produces
zeaxanthin in E. coli.
ACCESSION .
VERSION .
KEYWORDS pAC-ZEAXipi
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 10041)
AUTHORS Cunningham FX Jr, Gantt E
TITLE A portfolio of plasmids for identification and analysis of
carotenoid pathway enzymes: Adonis aestivalis as a case study.
JOURNAL Photosynth Res. 2007 May;92(2):245-59. Epub 2007 Jul 17.
PUBMED 17634749
REFERENCE 2 (bases 1 to 10041)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..10041
/organism="synthetic DNA construct"
/mol_type="other DNA"
primer_bind 134..153
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
promoter complement(220..322)
/label=cat promoter
/note="promoter of the E. coli cat gene encoding
chloramphenicol acetyltransferase"
primer_bind complement(714..731)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(848..1393)
/direction=LEFT
/label=p15A ori
/note="Plasmids containing the medium-copy-number p15A
origin of replication can be propagated in E. coli cells
that contain a second plasmid with the ColE1 origin.
"
primer_bind complement(928..947)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
promoter 1505..1533
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
CDS complement(9601..219)
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
ORIGIN
1 gaattccgga tgagcattca tcaggcgggc aagaatgtga ataaaggccg gataaaactt
61 gtgcttattt ttctttacgg tctttaaaaa ggccgtaata tccagctgaa cggtctggtt
121 ataggtacat tgagcaactg actgaaatgc ctcaaaatgt tctttacgat gccattggga
181 tatatcaacg gtggtatatc cagtgatttt tttctccatt ttagcttcct tagctcctga
241 aaatctcgat aactcaaaaa atacgcccgg tagtgatctt atttcattat ggtgaaagtt
301 ggaacctctt acgtgccgat caacgtctca ttttcgccaa aagttggccc agggcttccc
361 ggtatcaaca gggacaccag gatttattta ttctgcgaag tgatcttccg tcacaggtat
421 ttattcggcg caaagtgcgt cgggtgatgc tgccaactta ctgatttagt gtatgatggt
481 gtttttgagg tgctccagtg gcttctgttt ctatcagctg tccctcctgt tcagctactg
541 acggggtggt gcgtaacggc aaaagcaccg ccggacatca gcgctagcgg agtgtatact
601 ggcttactat gttggcactg atgagggtgt cagtgaagtg cttcatgtgg caggagaaaa
661 aaggctgcac cggtgcgtca gcagaatatg tgatacagga tatattccgc ttcctcgctc
721 actgactcgc tacgctcggt cgttcgactg cggcgagcgg aaatggctta cgaacggggc
781 ggagatttcc tggaagatgc caggaagata cttaacaggg aagtgagagg gccgcggcaa
841 agccgttttt ccataggctc cgcccccctg acaagcatca cgaaatctga cgctcaaatc
901 agtggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggcggctccc
961 tcgtgcgctc tcctgttcct gcctttcggt ttaccggtgt cattccgctg ttatggccgc
1021 gtttgtctca ttccacgcct gacactcagt tccgggtagg cagttcgctc caagctggac
1081 tgtatgcacg aaccccccgt tcagtccgac cgctgcgcct tatccggtaa ctatcgtctt
1141 gagtccaacc cggaaagaca tgcaaaagca ccactggcag cagccactgg taattgattt
1201 agaggagtta gtcttgaagt catgcgccgg ttaaggctaa actgaaagga caagttttgg
1261 tgactgcgct cctccaagcc agttacctcg gttcaaagag ttggtagctc agagaacctt
1321 cgaaaaaccg ccctgcaagg cggttttttc gttttcagag caagagatta cgcgcagacc
1381 aaaacgatct caagaagatc atcttattaa tcagataaaa tatttctaga tttcagtgca
1441 atttatctct tcaaatgtag cacctgaagt cagccccata cgatataagt tgtaattctc
1501 atgtttgaca gcttatcatc gataagcttt aatgcggtag tttatcacag ttaaattgct
1561 aacgcagtca ggcaccgtgt atgaaatcta acaatgcgct catcgtcatc ctcggcaccg
1621 tcaccctgga tgctgtaggc ataggcttgg ttatgccggt actgccgggc ctcttgcggg
1681 atatcgtcca ttccgacagc atcgccagtc actatggcgt gctgctagcg ctatatgcgt
1741 tgatgcaatt tctatgcgca cccgttctcg gagcactgtc cgaccgcttt ggccgccgcc
1801 cagtcctgct cgcttcgcta cttggagcca ctatcgacta cgcgatcatg gcgaccacac
1861 ccgtcctgtg gatctaaagg cacagcgtct catgcttcgc acaatgtaaa actgcttcag
1921 aacctggcga gagctatccg cgcggtctac ggttaactga tactaaaaga caattcagcg
1981 ggtaaccttg caatggtgag tggcagtaaa gcgggcgttt cgcctcatcg cgaaatagaa
2041 gtaatgagac aatccattga cgatcacctg gctggcctgt tacctgaaac cgacagccag
2101 gatatcgtca gccttgcgat gcgtgaaggc gtcatggcac ccggtaaacg gatccgtccg
2161 ctgctgatgc tgctggccgc ccgcgacctc cgctaccagg gcagtatgcc tacgctgctc
2221 gatctcgcct gcgccgttga actgacccat accgcgtcgc tgatgctcga cgacatgccc
2281 tgcatggaca acgccgagct gcgccgcggt cagcccacta cccacaaaaa atttggtgag
2341 agcgtggcga tccttgcctc cgttgggctg ctctctaaag cctttggtct gatcgccgcc
2401 accggcgatc tgccggggga gaggcgtgcc caggcggtca acgagctctc taccgccgtg
2461 ggcgtgcagg gcctggtact ggggcagttt cgcgatctta acgatgccgc cctcgaccgt
2521 acccctgacg ctatcctcag caccaaccac ctcaagaccg gcattctgtt cagcgcgatg
2581 ctgcagatcg tcgccattgc ttccgcctcg tcgccgagca cgcgagagac gctgcacgcc
2641 ttcgccctcg acttcggcca ggcgtttcaa ctgctggacg atctgcgtga cgatcacccg
2701 gaaaccggta aagatcgcaa taaggacgcg ggaaaatcga cgctggtcaa ccggctgggc
2761 gcagacgcgg cccggcaaaa gctgcgcgag catattgatt ccgccgacaa acacctcact
2821 tttgcctgtc cgcagggcgg cgccatccga cagtttatgc atctgtggtt tggccatcac
2881 cttgccgact ggtcaccggt catgaaaatc gcctgatacc gcccttttgg gttcaagcag
2941 tacataacga tggaaccaca ttacaggagt agtgatgaat gaaggacgag cgccttgttc
3001 agcgtaagaa cgatcatctg gatatcgttc tcgacccccg tcgcgccgta actcaggcta
3061 gcgcaggttt tgagcgctgg cgctttaccc actgcgccct gccagagctg aattttagcg
3121 acatcacgct ggaaaccacc ttcctgaatc ggcagctaca ggctccgctg ctgatcagct
3181 ccatgaccgg cggcgttgag cgctcgcgcc atatcaaccg ccacctcgcc gaggcggcgc
3241 aggtgctaaa aattgcgatg ggggtgggct cccagcgcgt cgccattgag agcgacgcgg
3301 gcttagggct ggataaaacc ctgcggcagc tggctccgga cgtgccgctg ctggcgaacc
3361 tcggcgcggc gcagctgacc ggcagaaaag gtattgatta cgcccgacgg gccgtggaga
3421 tgatcgaggc ggatgcgctg attgtgcacc taaacccgct gcaggaggcg ctacagcccg
3481 gcggcgatcg cgactggcgc ggacggctgg cggctattga aactctggtc cgcgagctgc
3541 ccgttccgct ggtggtgaaa gaggtgggag ccggtatctc ccgaaccgtg gccgggcagc
3601 tgatcgatgc cggcgttacc gtgattgacg tcgcgggcgc gggcggcacc agctgggccg
3661 ccgttgaagg cgagcgggcg gccaccgagc agcagcgcag cgtggccaac gtctttgccg
3721 actgggggat ccccaccgct gaggcgctgg ttgacattgc cgaggcctgg ccgcagatgc
3781 cccttattgc ctcgggcggg attaaaaacg gcgtcgacgc ggcgaaagcg ctgcggctcg
3841 gcgcgtgcat ggtagggcag gccgccgccg tgctcggcag cgcaggcgtc tccacggaga
3901 aggtgatcga tcacttcaac gtgattattg agcagctgcg ggtggcctgc ttctgcaccg
3961 gcagccgcag cctgagcgat ctaaagcagg ctgatatccg ctatgtgcgg gatacgccat
4021 gagccatttt gccattgtgg caccgccgct ctacagtcat gcggtggcgc tgcatgccct
4081 gtgggagcac tgcgctatca gccgccgccg caggtagaac gctcgccacg cagcacgccg
4141 cggatctttg cctcgctggg caccctccag ggccaccgtc tacgcctgtt tcagaagatc
4201 gcccgcgcct gtgccagcgt gggggcggag gtgaccattg cccactgcga tggcctgacg
4261 cccgcccagg ccgactcgct ctacgcctgc ggcgcgacgg aggtggtcag ctttgtcgac
4321 cagccgcgct acgttgccga ggctaatctg gtgatcaccc acggcggtct caataccgta
4381 ctggatgcgc tggctgccgc gacgccggtg ctggcggtgc cactctcttt cgaccagccc
4441 gccgtggctg cccggctggt ctataacggg ctgggtcgcc gggtatcgcg ctttgccaga
4501 cagcagacgc tggcggatga gattgcccaa ctgctggggg atgagacgct gcatcagcgt
4561 ctggcgacgg cccgccagca gcttaacgac gccgggggca cgccccgtgc ggcgaccctg
4621 attgaacagg ccatagcagg gagtgagagc gtatcgtgag ggatctgatt ttagtcggcg
4681 gcggcctggc caacgggctg atcgcctggc gtctgcgcca gcgctacccg cagcttaacc
4741 tgctgctgat cgaggccggg gagcagcccg gcgggaacca tacctggtca ttccatgaag
4801 acgatctgac tcccgggcag cacgcctggc tggccccgct ggtggcccac gcctggccgg
4861 gctatgaggt gcagtttccc gatcttcgcc gtcgcctcgc gcgcggctac tactccatta
4921 cctcagagcg ctttgccgag gccctgcatc aggcgctggg ggagaacatc tggctaaact
4981 gttcggtgag cgaggtgtta cccaatagcg tgcgccttgc caacggtgag gcgctgcttg
5041 ccggagcggt gattgacgga cgcggcgtga ccgccagttc ggcgatgcaa accggctatc
5101 agctctttct tggtcagcag tggcggctga cacagcccca cggcctgacc gtaccgatcc
5161 tgatggatgc cacggtggcg cagcagcagg gctatcgctt tgtctacacg ctgccgctct
5221 ccgccgacac gctgctgatc gaggatacgc gctacgccaa tgtcccgcag cgtgatgata
5281 atgccctacg ccagacggtt accgactatg ctcacagcaa agggtggcag ctggcccagc
5341 ttgaacgcga ggagaccggc tgtctgccga ttaccctggc gggtgacatc caggctctgt
5401 gggccgatgc gccgggcgtg ccgcgctcgg gaatgcgggc tgggctattt caccctacca
5461 ctggctattc gctgccgctg gcggtggccc ttgccgacgc gattgccgac agcccgcggc
5521 tgggcagcgt tccgctctat cagctcaccc ggcagtttgc cgaacgccac tggcgcaggc
5581 agggattctt ccgcctgctg aaccggatgc ttttcctggc cgggcgcgag gagaaccgct
5641 ggcgggtgat gcagcgcttt tatgggctgc cggagcccac cgtagagcgc ttttacgccg
5701 gtcggctctc tctctttgat aaggcccgca ttttgacggg caagccaccg gttccgctgg
5761 gcgaagcctg gcgggcggcg ctgaaccatt ttcctgacag acgagataaa ggatgaaaaa
5821 aaccgttgtg attggcgcag gctttggtgg cctggcgctg gcgattcgcc tgcaggcggc
5881 agggatccca accgtactgc tggagcagcg ggacaagccc ggcggtcggg cctacgtctg
5941 gcatgaccag ggctttacct ttgacgccgg gccgacggtg atcaccgatc ctaccgcgct
6001 tgaggcgctg ttcaccctgg ccggcaggcg catggaggat tacgtcaggc tgctgccggt
6061 aaaacccttc taccgactct gctgggagtc cgggaagacc ctcgactatg ctaacgacag
6121 cgccgagctt gaggcgcaga ttacccagtt caacccccgc gacgtcgagg gctaccggcg
6181 ctttctggct tactcccagg cggtattcca ggagggatat ttgcgcctcg gcagcgtgcc
6241 gttcctctct tttcgcgaca tgctgcgcgc cgggccgcag ctgcttaagc tccaggcgtg
6301 gcagagcgtc taccagtcgg tttcgcgctt tattgaggat gagcatctgc ggcaggcctt
6361 ctcgttccac tccctgctgg taggcggcaa ccccttcacc acctcgtcca tctacaccct
6421 gatccacgcc cttgagcggg agtggggggt ctggttccct gagggcggca ccggggcgct
6481 ggtgaacggc atggtgaagc tgtttaccga tctgggcggg gagatcgaac tcaacgcccg
6541 ggtcgaagag ctggtggtgg ccgataaccg cgtaagccag gtccggctgg cggatggtcg
6601 gatctttgac accgacgccg tagcctcgaa cgctgacgtg gtgaacacct ataaaaagct
6661 gctcggccac catccggtgg ggcagaagcg ggcggcagcg ctggagcgca agagcatgag
6721 caactcgctg tttgtgctct acttcggcct gaaccagcct cattcccagc tggcgcacca
6781 taccatctgt tttggtcccc gctaccggga gctgatcgac gagatcttta ccggcagcgc
6841 gctggcggat gacttctcgc tctacctgca ctcgccctgc gtgaccgatc cctcgctcgc
6901 gcctcccggc tgcgccagct tctacgtgct ggccccggtg ccgcatcttg gcaacgcgcc
6961 gctggactgg gcgcaggagg ggccgaagct gcgcgaccgc atctttgact accttgaaga
7021 gcgctatatg cccggcctgc gtagccagct ggtgacccag cggatcttta ccccggcaga
7081 cttccacgac acgctggatg cgcatctggg atcggccttc tccatcgagc cgctgctgac
7141 ccaaagcgcc tggttccgcc cgcacaaccg cgacagcgac attgccaacc tctacctggt
7201 gggcgcaggt actcaccctg gggcgggcat tcctggcgta gtggcctcgg cgaaagccac
7261 cgccagcctg atgattgagg atctgcaatg agccaaccgc cgctgcttga ccacgccacg
7321 cagaccatgg ccaacggctc gaaaagtttt gccaccgctg cgaagctgtt cgacccggcc
7381 acccgccgta gcgtgctgat gctctacacc tggtgccgcc actgcgatga cgtcattgac
7441 gaccagaccc acggcttcgc cagcgaggcc gcggcggagg aggaggccac ccagcgcctg
7501 gcccggctgc gcacgctgac cctggcggcg tttgaagggg ccgagatgca ggatccggcc
7561 ttcgctgcct ttcaggaggt ggcgctgacc cacggtatta cgccccgcat ggcgctcgat
7621 cacctcgacg gctttgcgat ggacgtggct cagacccgct atgtcacctt tgaggatacg
7681 ctgcgctact gctatcacgt ggcgggcgtg gtgggtctga tgatggccag ggtgatgggc
7741 gtgcgggatg agcgggtgct ggatcgcgcc tgcgatctgg ggctggcctt ccagctgacg
7801 aatatcgccc gggatattat tgacgatgcg gctattgacc gctgctatct gcccgccgag
7861 tggctgcagg atgccgggct gaccccggag aactatgccg cgcgggagaa tcgggccgcg
7921 ctggcgcggg tggcggagcg gcttattgat gccgcagagc cgtactacat ctcctcccag
7981 gccgggctac acgatctgcc gccgcgctgc gcctgggcga tcgccaccgc ccgcagcgtc
8041 taccgggaga tcggtattaa ggtaaaagcg gcgggaggca gcgcctggga tcgccgccag
8101 cacaccagca aaggtgaaaa aattgccatg ctgatggcgg caccggggca ggttattcgg
8161 gcgaagacga cgagggtgac gccgcgtccg gccggtcttt ggcagcgtcc cgtttaggcg
8221 ggcggccatg acgttcacgc aggatcgcct gtaggtcggc aggcttgcgg gcgtaaataa
8281 aaccgaagga gacgcagccc tcccggccgc gcaccgcgtg gtgcaggcgg tgggcgacgt
8341 agagccgctt caggtagccc cggcgcggga tccagtggaa gggccagcgc tgatgcacca
8401 gaccgtcgtg caccaggaag tagagcaggc catagaccgt catgccgcag ccaatccact
8461 gcaggggcca aacgcccgcc gtgcccacgg caatcagcgc gatagccacc ccggcaaaca
8521 ccaccgcaaa gagatcgttt agctcaaata cgcccttgcg cggggtatgg tgtgactcat
8581 gccagcgcca tccccagccg tgcataatgt agcggtgggt aaacgcggcg atgccctcca
8641 tcgcaataac gctcaagatg acgattaaac tatttactag catagcggtg cgcgctcctg
8701 cgactcagac tggcttaatc aaaaccctta gcctctaagc atagtcgcaa tcgcaggcgg
8761 cgcgcatcgg tatcaggcca gagcctgctg cagatccgcc agcagatcct tctcatcctc
8821 aatgcccacc gacaggcgga tcagctgcgg cgtaatgccg ttcgccaggc gctgcgccag
8881 cgggatcgag gcgtgggtca tactgaacgg ctggctcacc aggctctcaa cgccgcccag
8941 gctctcagcg agggtaaaca gcttcagttt actgatcacc tctgccgcac gctggtcgtc
9001 gcctttgatc acgatggaga tcatgccgcc cggctgagcc atctgctggc gcgcgagggc
9061 atactgcggg tgggatgtca gccacgggaa ccagactttc tcgacctgcg gctgctgctc
9121 aagccagcgg gccagcgcca gggcgttgct gctgtggcgc tcgatgcgca gggcgagggt
9181 gcggatcccg cgcagggtca ggaagctgct gaacggatcg agcacgccgc ccaccgcatt
9241 ttgcaggtag cccagcttct ccgccagcgc cggacgatcg cccaccaccg ccagcccggc
9301 gaccacgtcg gagtggccgt tgaggtattt ggtggccgag tgcaccacga tatcaaagcc
9361 cagcgtcagc gggcggtgga tcagcggcga ggcaaaggta ttatctgcca cgctaatcac
9421 gttatgacgg cgggcgatct cagcgatagc cgccaggtcc gccagcttca acagcgggtt
9481 ggtgggggtc tctacccggg tcgaatttgc tttcgaattt ctgccattca tccgcttatt
9541 atcacttatt caggcgtagc aaccaggcgt ttaagggcac caataactgc cttaaaaaaa
9601 ttacgccccg ccctgccact catcgcagta ctgttgtaat tcattaagca ttctgccgac
9661 atggaagcca tcacagacgg catgatgaac ctgaatcgcc agcggcatca gcaccttgtc
9721 gccttgcgta taatatttgc ccatggtgaa aacgggggcg aagaagttgt ccatattggc
9781 cacgtttaaa tcaaaactgg tgaaactcac ccagggattg gctgagacga aaaacatatt
9841 ctcaataaac cctttaggga aataggccag gttttcaccg taacacgcca catcttgcga
9901 atatatgtgt agaaactgcc ggaaatcgtc gtggtattca ctccagagcg atgaaaacgt
9961 ttcagtttgc tcatggaaaa cggtgtaaca agggtgaaca ctatcccata tcaccagctc
10021 accgtctttc attgccatac g
//
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