| 质粒名称: | pLVX-EF1alpha-SARS-CoV-2-orf9c-IRES-Puro |
|---|---|
| 出品公司: | Addgene |
| 目录编号: | 141393 |
| 存储实验室: | Nevan Krogan |
|
载体骨架基本信息 |
|
| 载体名称: | pLVX-EF1alpha-IRES-Puro |
| 载体出品公司: | Takara Bio |
| 空载体大小: | 8825bp |
| 完整质粒大小: | 9126 bp |
| 载体类型: | 哺乳细胞表达, Lentiviral |
| 筛选标记: | Puromycin |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 30 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 未知 |
|
插入基因信息 |
|
| 插入基因名称: | SARS-CoV-2-orf9c |
| 物种来源: | SARS-CoV-2 |
| 基因突变: | human codon optimized |
| 启动子: | EF1alpha |
| 标签/融合蛋白: | 2xStrep (N terminal on insert) |
| 克隆方法: | 未知 |
|
文献引用方法 |
|
| 材料和方法部分: | pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro was a gift from Nevan Krogan (Addgene plasmid # 141393 ; http://n2t.net/addgene:141393 ; RRID:Addgene_141393) |
| 参考文献部分: | A SARS-CoV-2 protein interaction map reveals targets for drug repurposing. Gordon DE, Jang GM, Bouhaddou M, Xu J, Obernier K, White KM, O'Meara MJ, Rezelj VV, Guo JZ, Swaney DL, Tummino TA, Huttenhain R, Kaake RM, Richards AL, Tutuncuoglu B, Foussard H, Batra J, Haas K, Modak M, Kim M, Haas P, Polacco BJ, Braberg H, Fabius JM, Eckhardt M, Soucheray M, Bennett MJ, Cakir M, McGregor MJ, Li Q, Meyer B, Roesch F, Vallet T, Mac Kain A, Miorin L, Moreno E, Naing ZZC, Zhou Y, Peng S, Shi Y, Zhang Z, Shen W, Kirby IT, Melnyk JE, Chorba JS, Lou K, Dai SA, Barrio-Hernandez I, Memon D, Hernandez-Armenta C, Lyu J, Mathy CJP, Perica T, Pilla KB, Ganesan SJ, Saltzberg DJ, Rakesh R, Liu X, Rosenthal SB, Calviello L, Venkataramanan S, Liboy-Lugo J, Lin Y, Huang XP, Liu Y, Wankowicz SA, Bohn M, Safari M, Ugur FS, Koh C, Savar NS, Tran QD, Shengjuler D, Fletcher SJ, O'Neal MC, Cai Y, Chang JCJ, Broadhurst DJ, Klippsten S, Sharp PP, Wenzell NA, Kuzuoglu-Ozturk D, Wang HY, Trenker R, Young JM, Cavero DA, Hiatt J, Roth TL, Rathore U, Subramanian A, Noack J, Hubert M, Stroud RM, Frankel AD, Rosenberg OS, Verba KA, Agard DA, Ott M, Emerman M, Jura N, von Zastrow M, Verdin E, Ashworth A, Schwartz O, d'Enfert C, Mukherjee S, Jacobson M, Malik HS, Fujimori DG, Ideker T, Craik CS, Floor SN, Fraser JS, Gross JD, Sali A, Roth BL, Ruggero D, Taunton J, Kortemme T, Beltrao P, Vignuzzi M, Garcia-Sastre A, Shokat KM, Shoichet BK, Krogan NJ. Nature. 2020 Jul;583(7816):459-468. doi: 10.1038/s41586-020-2286-9. Epub 2020 Apr 30. 10.1038/s41586-020-2286-9 PubMed 32353859 |
买家导航
pLVX-EF1alpha-SARS-CoV-2-orf9c-IRES-Puro载体质粒基本信息
pLVX-EF1alpha-SARS-CoV-2-orf9c-IRES-Puro质粒图谱载体图谱和pLVX-EF1alpha-SARS-CoV-2-orf9c-IRES-Puro载体序列质粒序列多克隆位点信息
pLVX-EF1alpha-SARS-CoV-2-orf9c-IRES-Puro质粒载体简介
pLVX-EF1alpha-SARS-CoV-2-orf9c-IRES-Puro质粒序列载体序列
LOCUS pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro 9126 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Lentiviral expression of SARS-CoV-2 protein; transient expression
and generate lentivirus.
ACCESSION .
VERSION .
KEYWORDS pLXV-EF1alpha-2xStrep-SARS-CoV-2-orf9c-IRES-Puro
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9126)
AUTHORS Gordon DE, et al.
TITLE A SARS-CoV-2-Human Protein-Protein Interaction Map Reveals Drug
Targets and Potential Drug Repurposing
JOURNAL BioRxiv March 2020
REFERENCE 2 (bases 1 to 9126)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9126
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 1..1182
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 231..1173
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1130..1150
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
CDS 1216..1239
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
CDS 1276..1299
/codon_start=1
/product="peptide that binds Strep-Tactin(R), an engineered
form of streptavidin"
/label=Strep-Tag II
/translation="WSHPQFEK"
misc_feature 1539..2112
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(1706..1723)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 1933..1952
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 2145..2744
/codon_start=1
/gene="pac from Streptomyces alboniger"
/product="puromycin N-acetyltransferase"
/label=PuroR
/note="confers resistance to puromycin"
/translation="MTEYKPTVRLATRDDVPRAVRTLAAAFADYPATRHTVDPDRHIER
VTELQELFLTRVGLDIGKVWVADDGAAVAVWTTPESVEAGAVFAEIGPRMAELSGSRLA
AQQQMEGLLAPHRPKEPAWFLATVGVSPDHQGKGLGSAVVLPGVEAAERAGVPAFLETS
APRNLPFYERLGFTVTADVEVPEGPRTWCMTRKPGA"
primer_bind complement(2145..2164)
/label=Puro-R
/note="Puromycin resistance gene, reverse primer. Also
called puro-variant-R"
primer_bind 2641..2661
/label=Puro-F
/note="Puromycin resistance gene, forward primer"
misc_feature 2758..3346
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
primer_bind complement(2811..2831)
/label=WPRE-R
/note="WPRE, reverse primer"
CDS complement(3229..3240)
/codon_start=1
/product="Factor Xa recognition and cleavage site"
/label=Factor Xa site
/translation="IEGR"
LTR 3553..4186
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
primer_bind complement(4314..4330)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4314..4330)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(4327..4349)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 4338..4354
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4362..4392)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4407..4428
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
primer_bind complement(4545..4562)
/label=L4440
/note="L4440 vector, forward primer"
rep_origin complement(4716..5304)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind complement(4796..4815)
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
CDS complement(5475..6335)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind 6098..6117
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
promoter complement(6336..6440)
/gene="bla"
/label=AmpR promoter
polyA_signal 6488..6622
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(6525..6544)
/label=SV40pA-R
/note="SV40 polyA, reverse primer"
primer_bind 6579..6598
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
LTR 6790..7423
/label=3' LTR
/note="3' long terminal repeat (LTR) from HIV-1"
misc_feature 7470..7595
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 8092..8325
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
CDS 8510..8554
/codon_start=1
/product="antigenic peptide corresponding to amino acids
655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik
et al., 2013)"
/label=gp41 peptide
/note="recognized by the 2H10 single-chain llama nanobody"
/translation="KNEQELLELDKWASL"
misc_feature 8817..8932
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1 (lacking the first T)"
ORIGIN
1 gctccggtgc ccgtcagtgg gcagagcgca catcgcccac agtccccgag aagttggggg
61 gaggggtcgg caattgaacc ggtgcctaga gaaggtggcg cggggtaaac tgggaaagtg
121 atgtcgtgta ctggctccgc ctttttcccg agggtggggg agaaccgtat ataagtgcag
181 tagtcgccgt gaacgttctt tttcgcaacg ggtttgccgc cagaacacag gtaagtgccg
241 tgtgtggttc ccgcgggcct ggcctcttta cgggttatgg cccttgcgtg ccttgaatta
301 cttccacgcc cctggctgca gtacgtgatt cttgatcccg agcttcgggt tggaagtggg
361 tgggagagtt cgaggccttg cgcttaagga gccccttcgc ctcgtgcttg agttgaggcc
421 tggcttgggc gctggggccg ccgcgtgcga atctggtggc accttcgcgc ctgtctcgct
481 gctttcgata agtctctagc catttaaaat ttttgatgac ctgctgcgac gctttttttc
541 tggcaagata gtcttgtaaa tgcgggccaa gatctgcaca ctggtatttc ggtttttggg
601 gccgcgggcg gcgacggggc ccgtgcgtcc cagcgcacat gttcggcgag gcggggcctg
661 cgagcgcggc caccgagaat cggacggggg tagtctcaag ctggccggcc tgctctggtg
721 cctggcctcg cgccgccgtg tatcgccccg ccctgggcgg caaggctggc ccggtcggca
781 ccagttgcgt gagcggaaag atggccgctt cccggccctg ctgcagggag ctcaaaatgg
841 aggacgcggc gctcgggaga gcgggcgggt gagtcaccca cacaaaggaa aagggccttt
901 ccgtcctcag ccgtcgcttc atgtgactcc acggagtacc gggcgccgtc caggcacctc
961 gattagttct cgagcttttg gagtacgtcg tctttaggtt ggggggaggg gttttatgcg
1021 atggagtttc cccacactga gtgggtggag actgaagtta ggccagcttg gcacttgatg
1081 taattctcct tggaatttgc cctttttgag tttggatctt ggttcattct caagcctcag
1141 acagtggttc aaagtttttt tcttccattt caggtgtcgt gaggatctat ttccggtgaa
1201 ttcgccgcca ccatgtggtc ccatccgcag tttgagaagg gtggtggttc aggcggaggc
1261 tccgggggcg ggagctggtc tcacccgcaa tttgaaaaag gcgctgcggc tcttcaatcc
1321 tgctataact tcttgaaaga gcaacactgc caaaaggcga gcacacagaa aggagccgag
1381 gcagccgtaa agccgcttct tgtccctcat cacgttgtcg ccacagtcca agaaatacag
1441 cttcaagcgg ccgttggcga gcttctgctc ctcgaatggc tcgcaatggc ggtgatgttg
1501 ttgctgttgt gctgctgtct tacggattga ggatcccgcc cctctccctc ccccccccct
1561 aacgttactg gccgaagccg cttggaataa ggccggtgtg cgtttgtcta tatgttattt
1621 tccaccatat tgccgtcttt tggcaatgtg agggcccgga aacctggccc tgtcttcttg
1681 acgagcattc ctaggggtct ttcccctctc gccaaaggaa tgcaaggtct gttgaatgtc
1741 gtgaaggaag cagttcctct ggaagcttct tgaagacaaa caacgtctgt agcgaccctt
1801 tgcaggcagc ggaacccccc acctggcgac aggtgcctct gcggccaaaa gccacgtgta
1861 taagatacac ctgcaaaggc ggcacaaccc cagtgccacg ttgtgagttg gatagttgtg
1921 gaaagagtca aatggctctc ctcaagcgta ttcaacaagg ggctgaagga tgcccagaag
1981 gtaccccatt gtatgggatc tgatctgggg cctcggtgca catgctttac atgtgtttag
2041 tcgaggttaa aaaaacgtct aggccccccg aaccacgggg acgtggtttt cctttgaaaa
2101 acacgatgat aagcttgcca caacccacaa ggagacgacc ttccatgacc gagtacaagc
2161 ccacggtgcg cctcgccacc cgcgacgacg tcccccgggc cgtacgcacc ctcgccgccg
2221 cgttcgccga ctaccccgcc acgcgccaca ccgtcgaccc ggaccgccac atcgagcggg
2281 tcaccgagct gcaagaactc ttcctcacgc gcgtcgggct cgacatcggc aaggtgtggg
2341 tcgcggacga cggcgccgcg gtggcggtct ggaccacgcc ggagagcgtc gaagcggggg
2401 cggtgttcgc cgagatcggc ccgcgcatgg ccgagttgag cggttcccgg ctggccgcgc
2461 agcaacagat ggaaggcctc ctggcgccgc accggcccaa ggagcccgcg tggttcctgg
2521 ccaccgtcgg cgtctcgccc gaccaccagg gcaagggtct gggcagcgcc gtcgtgctcc
2581 ccggagtgga ggcggccgag cgcgccgggg tgcccgcctt cctggagacc tccgcgcccc
2641 gcaacctccc cttctacgag cggctcggct tcaccgtcac cgccgacgtc gaggtgcccg
2701 aaggaccgcg cacctggtgc atgacccgca agcccggtgc ctagacgcgt ctggaacaat
2761 caacctctgg attacaaaat ttgtgaaaga ttgactggta ttcttaacta tgttgctcct
2821 tttacgctat gtggatacgc tgctttaatg cctttgtatc atgctattgc ttcccgtatg
2881 gctttcattt tctcctcctt gtataaatcc tggttgctgt ctctttatga ggagttgtgg
2941 cccgttgtca ggcaacgtgg cgtggtgtgc actgtgtttg ctgacgcaac ccccactggt
3001 tggggcattg ccaccacctg tcagctcctt tccgggactt tcgctttccc cctccctatt
3061 gccacggcgg aactcatcgc cgcctgcctt gcccgctgct ggacaggggc tcggctgttg
3121 ggcactgaca attccgtggt gttgtcgggg aagctgacgt cctttccatg gctgctcgcc
3181 tgtgttgcca cctggattct gcgcgggacg tccttctgct acgtcccttc ggccctcaat
3241 ccagcggacc ttccttcccg cggcctgctg ccggctctgc ggcctcttcc gcgtcttcgc
3301 cttcgccctc agacgagtcg gatctccctt tgggccgcct ccccgcctgg aattaattct
3361 gcagtcgaga cctagaaaaa catggagcaa tcacaagtag caatacagca gctaccaatg
3421 ctgattgtgc ctggctagaa gcacaagagg aggaggaggt gggttttcca gtcacacctc
3481 aggtaccttt aagaccaatg acttacaagg cagctgtaga tcttagccac tttttaaaag
3541 aaaagagggg actggaaggg ctaattcact cccaacgaag acaagatatc cttgatctgt
3601 ggatctacca cacacaaggc tacttccctg attagcagaa ctacacacca gggccagggg
3661 tcagatatcc actgaccttt ggatggtgct acaagctagt accagttgag ccagataagg
3721 tagaagaggc caataaagga gagaacacca gcttgttaca ccctgtgagc ctgcatggga
3781 tggatgaccc ggagagagaa gtgttagagt ggaggtttga cagccgccta gcatttcatc
3841 acgtggcccg agagctgcat ccggagtact tcaagaactg ctgatatcga gcttgctaca
3901 agggactttc cgctggggac tttccaggga ggcgtggcct gggcgggact ggggagtggc
3961 gagccctcag atcctgcata taagcagctg ctttttgcct gtactgggtc tctctggtta
4021 gaccagatct gagcctggga gctctctggc taactaggga acccactgct taagcctcaa
4081 taaagcttgc cttgagtgct tcaagtagtg tgtgcccgtc tgttgtgtga ctctggtaac
4141 tagagatccc tcagaccctt ttagtcagtg tggaaaatct ctagcagtag tagttcatgt
4201 catcttatta ttcagtattt ataacttgca aagaaatgaa tatcagagag tgagaggcct
4261 tgacattgct agcgtttacc gtcgacctct agctagagct tggcgtaatc atggtcatag
4321 ctgtttcctg tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc
4381 ataaagtgta aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc
4441 tcactgcccg ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa
4501 cgcgcgggga gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg
4561 ctgcgctcgg tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg
4621 ttatccacag aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag
4681 gccaggaacc gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac
4741 gagcatcaca aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga
4801 taccaggcgt ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt
4861 accggatacc tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc
4921 tgtaggtatc tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc
4981 cccgttcagc ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta
5041 agacacgact tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat
5101 gtaggcggtg ctacagagtt cttgaagtgg tggcctaact acggctacac tagaagaaca
5161 gtatttggta tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct
5221 tgatccggca aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt
5281 acgcgcagaa aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct
5341 cagtggaacg aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc
5401 acctagatcc ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa
5461 acttggtctg acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta
5521 tttcgttcat ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc
5581 ttaccatctg gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat
5641 ttatcagcaa taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta
5701 tccgcctcca tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt
5761 aatagtttgc gcaacgttgt tgccattgct acaggcatcg tggtgtcacg ctcgtcgttt
5821 ggtatggctt cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg
5881 ttgtgcaaaa aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc
5941 gcagtgttat cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc
6001 gtaagatgct tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg
6061 cggcgaccga gttgctcttg cccggcgtca atacgggata ataccgcgcc acatagcaga
6121 actttaaaag tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta
6181 ccgctgttga gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct
6241 tttactttca ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag
6301 ggaataaggg cgacacggaa atgttgaata ctcatactct tcctttttca atattattga
6361 agcatttatc agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat
6421 aaacaaatag gggttccgcg cacatttccc cgaaaagtgc cacctgacgt cgacggatcg
6481 ggagatcaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
6541 tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
6601 tgtatcttat catgtctgga tcaactggat aactcaagct aaccaaaatc atcccaaact
6661 tcccacccca taccctatta ccactgccaa ttacctgtgg tttcatttac tctaaacctg
6721 tgattcctct gaattatttt cattttaaag aaattgtatt tgttaaatat gtactacaaa
6781 cttagtagtt ggaagggcta attcactccc aaagaagaca agatatcctt gatctgtgga
6841 tctaccacac acaaggctac ttccctgatt agcagaacta cacaccaggg ccaggggtca
6901 gatatccact gacctttgga tggtgctaca agctagtacc agttgagcca gataaggtag
6961 aagaggccaa taaaggagag aacaccagct tgttacaccc tgtgagcctg catgggatgg
7021 atgacccgga gagagaagtg ttagagtgga ggtttgacag ccgcctagca tttcatcacg
7081 tggcccgaga gctgcatccg gagtacttca agaactgctg atatcgagct tgctacaagg
7141 gactttccgc tggggacttt ccagggaggc gtggcctggg cgggactggg gagtggcgag
7201 ccctcagatc ctgcatataa gcagctgctt tttgcctgta ctgggtctct ctggttagac
7261 cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa
7321 agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag
7381 agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc ccgaacaggg
7441 acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg cttgctgaag
7501 cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt ttgactagcg
7561 gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg agaattagat
7621 cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa ttaaaacata
7681 tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg ttagaaacat
7741 cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca ggatcagaag
7801 aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa aggatagaga
7861 taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa agtaagacca
7921 ccgcacagca agcggccggc cgctgatctt cagacctgga ggaggagata tgagggacaa
7981 ttggagaagt gaattatata aatataaagt agtaaaaatt gaaccattag gagtagcacc
8041 caccaaggca aagagaagag tggtgcagag agaaaaaaga gcagtgggaa taggagcttt
8101 gttccttggg ttcttgggag cagcaggaag cactatgggc gcagcgtcaa tgacgctgac
8161 ggtacaggcc agacaattat tgtctggtat agtgcagcag cagaacaatt tgctgagggc
8221 tattgaggcg caacagcatc tgttgcaact cacagtctgg ggcatcaagc agctccaggc
8281 aagaatcctg gctgtggaaa gatacctaaa ggatcaacag ctcctgggga tttggggttg
8341 ctctggaaaa ctcatttgca ccactgctgt gccttggaat gctagttgga gtaataaatc
8401 tctggaacag atttggaatc acacgacctg gatggagtgg gacagagaaa ttaacaatta
8461 cacaagctta atacactcct taattgaaga atcgcaaaac cagcaagaaa agaatgaaca
8521 agaattattg gaattagata aatgggcaag tttgtggaat tggtttaaca taacaaattg
8581 gctgtggtat ataaaattat tcataatgat agtaggaggc ttggtaggtt taagaatagt
8641 ttttgctgta ctttctatag tgaatagagt taggcaggga tattcaccat tatcgtttca
8701 gacccacctc ccaaccccga ggggacccga caggcccgaa ggaatagaag aagaaggtgg
8761 agagagagac agagacagat ccattcgatt agtgaacgga tctcgacggt atcgccttta
8821 aaagaaaagg ggggattggg gggtacagtg caggggaaag aatagtagac ataatagcaa
8881 cagacataca aactaaagaa ttacaaaaac aaattacaaa aattcaaaat tttcgggttt
8941 attacaggga cagcagagat ccagtttatc gatgagtaat tcatacaaaa ggactcgccc
9001 ctgccttggg gaatcccagg gaccgtcgtt aaactcccac taacgtagaa cccagagatc
9061 gctgcgttcc cgccccctca cccgcccgct ctcgtcatca ctgaggtgga gaagagcatg
9121 cgtgag
//
