| 质粒名称: | pSL1342 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002432 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for a LIRP variant where amino acid residues A54 and G55 were deleted from MCP(LOV2 53/54) (PhCMV -MCP(S-LOV2 53/54)-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: |
PhCMV
|
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | MCP(S-LOV2 53/54) |
| 物种来源: |
synthetic
|
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
Rationally designed light-inducible RNA-releasing protein for translational regulation and optogenetic control of gene therapies
NA
NA
|
pSL1342
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pSL1342载体质粒基本信息
pSL1342质粒图谱载体图谱和pSL1342载体序列质粒序列多克隆位点信息
pSL1342质粒载体简介
pSL1342质粒序列载体序列
LOCUS pSL1342 6207 bp DNA circular SYN 05-MAR-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6207)
AUTHORS Trial User
TITLE Direct Submission
JOURNAL Exported Aug 28, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6207
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 232..819
/label=CMV
enhancer 235..614
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
primer_bind 863..882
/label=T7
promoter 863..881
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 880..923
/label=oSCL2210
primer_bind 886..918
/label=Oscl32-1
primer_bind 886..918
/label=oSCL1118
primer_bind 901..918
/label=Oscl42-1
regulatory 901..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
regulatory 902..910
/label=Kozak sequence
/note="vertebrate consensus sequence for strong initiation
of translation (Kozak, 1987)"
/regulatory_class="other"
CDS 910..1065
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="ASNFTQFVLVDNGGTGDVTVAPSNFANGVAEWISSNSRSQAYKVT
CSVRQSS"
primer_bind complement(1004..1082)
/label=oSCL2215
misc_feature 1023^1024
/label=Feature 16
primer_bind 1024..1043
/label=oSCL2216
primer_bind 1043..1083
/label=oSCL2328
primer_bind 1043..1083
/label=oSCL2329
primer_bind 1043..1083
/label=oSCL2330
primer_bind complement(1043..1062)
/label=oSCL2327
primer_bind complement(1051..1082)
/label=oSCL2217
primer_bind 1064..1083
/label=oSCL1757
primer_bind complement(1064..1082)
/label=oSCL2219
misc_feature 1066..1476
/label=LOV2
primer_bind complement(1455..1476)
/label=oSCL1758
primer_bind 1457..1503
/label=oSCL2218
primer_bind 1457..1476
/label=oSCL2216
primer_bind 1457..1476
/label=oSCL2220
CDS 1477..1704
/codon_start=1
/product="bacteriophage MS2 coat protein"
/label=MS2-N55K
/note="binds with high affinity to a specific stem-loop
structure in the viral RNA (Lim et al., 1994)"
/translation="QKRKYTIKVEVPKVATQTVGGVELPVAAWRSYLNMELTIPIFATN
SDCELIVKAMQGLLKDGNPIPSAIAANSGIY"
misc_feature 1477
/label=Feature 17
primer_bind complement(1578..1599)
/label=oSCL2219
primer_bind 1599..1619
/label=oSCL2220
misc_feature 1599^1600
/label=Feature 18
primer_bind complement(1684..1727)
/label=oSCL2211
primer_bind complement(1684..1713)
/label=Oscl32-2
primer_bind complement(1684..1704)
/label=oSCL093
primer_bind complement(1684..1704)
/label=oSCL396
terminator 1804..2031
/label=bGH PA
polyA_signal 1807..2031
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
rep_origin 2077..2505
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2519..2848
/label=SV40 promoter
/note="SV40 enhancer and early promoter"
rep_origin 2699..2834
/label=SV40 ori
/note="SV40 origin of replication"
misc_feature 2699..2776
/label=SV40 ORI
CDS 2915..3709
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
CDS 2918..3709
/label=Kan/neoR
polyA_signal 3883..4004
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
primer_bind complement(4049..4069)
/label=M13-rev
primer_bind complement(4053..4069)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
misc_binding complement(4075..4097)
/label=LacO
protein_bind 4077..4093
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4101..4131)
/label=lac promoter
/note="promoter for the E. coli lac operon"
promoter complement(4102..4131)
/label=lac
protein_bind 4146..4167
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4455..5040)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5211..6071)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
CDS complement(5214..5873)
/label=AmpR
promoter complement(6072..6176)
/gene="bla"
/label=AmpR promoter
promoter complement(6113..6141)
/label=Amp prom
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gccaccatgg cttcaaactt tactcagttc gtgctcgtgg acaatggtgg gacaggggat
961 gtgacagtgg ctccttctaa tttcgctaat ggggtggcag agtggatcag ctccaactca
1021 cggagccagg cctacaaggt gacatgcagc gtcaggcagt ctagtttgga acgtatcgaa
1081 aagaattttg tcatcacgga cccgcgtctg cccgacaatc cgattatctt cgcgtcagac
1141 tctttcttac aactgactga gtatagtaga gaggagatat tggggcgtaa ctgtagattt
1201 cttcaggggc cagaaactga tcgggctacc gttcgcaaga tacgtgacgc aatagacaac
1261 cagaccgagg tgacggtgca gctgattaac tacacaaagt ctgggaagaa gttctggaac
1321 ctgtttcatt tacaacctat gagggaccaa aaaggtgacg ttcaatattt catcggggtt
1381 cagttagatg ggactgagca cgtgagagat gcagcagaaa gagagggtgt aatgcttatt
1441 aaaaaaacag ccgagaatat cgacgaagcc gctggtcaga agagaaagta taccatcaag
1501 gtggaggtcc ccaaagtggc tacccagaca gtgggcggag tcgaactgcc tgtcgccgct
1561 tggaggtcct acctgaacat ggagctcact atcccaattt tcgctaccaa ttctgactgt
1621 gaactcatcg tgaaggcaat gcaggggctc ctcaaagacg gtaatcctat cccttccgcc
1681 atcgccgcta actcaggtat ctactaagga tccactagtc cagtgtggtg gaattctgca
1741 gatatccagc acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag
1801 cctcgactgt gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct
1861 tgaccctgga aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc
1921 attgtctgag taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg
1981 aggattggga agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg
2041 cggaaagaac cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa
2101 gcgcggcggg tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc
2161 ccgctccttt cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag
2221 ctctaaatcg ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca
2281 aaaaacttga ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc
2341 gccctttgac gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa
2401 cactcaaccc tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct
2461 attggttaaa aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt
2521 gtgtcagtta gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat
2581 gcatctcaat tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag
2641 tatgcaaagc atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat
2701 cccgccccta actccgccca gttccgccca ttctccgccc catggctgac taattttttt
2761 tatttatgca gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg
2821 cttttttgga ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg
2881 atctgatcaa gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc
2941 aggttctccg gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat
3001 cggctgctct gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt
3061 caagaccgac ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg
3121 gctggccacg acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag
3181 ggactggctg ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc
3241 tgccgagaaa gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc
3301 tacctgccca ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga
3361 agccggtctt gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga
3421 actgttcgcc aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg
3481 cgatgcctgc ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg
3541 tggccggctg ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc
3601 tgaagagctt ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc
3661 cgattcgcag cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg
3721 gggttcgaaa tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc
3781 gccgccttct atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc
3841 ctccagcgcg gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct
3901 tataatggtt acaaataaag caatagcatc acaaatttca caaataaagc atttttttca
3961 ctgcattcta gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg
4021 tcgacctcta gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt
4081 tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt
4141 gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg
4201 ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg
4261 cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg
4321 cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat
4381 aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc
4441 gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc
4501 tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga
4561 agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt
4621 ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg
4681 taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc
4741 gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg
4801 gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc
4861 ttgaagtggt ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg
4921 ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc
4981 gctggtagcg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
5041 gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
5101 gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
5161 tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
5221 ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
5281 ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
5341 atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
5401 ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
5461 tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
5521 attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
5581 tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
5641 ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
5701 gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
5761 gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
5821 gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
5881 aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
5941 taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
6001 tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
6061 tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
6121 atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
6181 tttccccgaa aagtgccacc tgacgtc
//
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