| 出品公司: | Agilent |
|---|---|
| 载体名称: | pAAV-RC8 |
| 质粒类型: | 哺乳动物细胞载体；腺病毒包装载体 |
| 高拷贝/低拷贝: | 高拷贝 |
| 克隆方法: | 限制性内切酶，多克隆位点 |
| 启动子: | -- |
| 载体大小: | 7.3 kb |
| 5’测序引物: | M13-F: TGTAAAACGACGGCCAGT |
| 3’测序引物: | M13-R: CAGGAAACAGCTATGAC |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 克隆菌株: | DH5 alpha等 |
| 宿主细胞（系）: | 常规细胞系（293、CV-1、CHO等） |
| 产品目录号: | -- |
| 稳定性: | 瞬表达 |
| 组成型/诱导型: | 组成型 |
| 病毒/非病毒: | 非病毒 |
买家导航
pAAV-RC8载体 pAAVRC8质粒基本信息
pAAV-RC8质粒图谱 pAAVRC8载体图谱和pAAV-RC8载体序列 pAAVRC8质粒序列多克隆位点信息
pAAV-RC8质粒 pAAVRC8载体简介
pAAV-RC8质粒序列 pAAVRC8载体序列
LOCUS pAAV-RC8 7336 bp DNA circular SYN 22-OCT-2018
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 7336)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Feb 11, 2025 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..7336
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 142..158
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 168..186
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
primer_bind 168..186
/label=T7(生工常备）
primer_bind complement(176..214)
/label=com-R
primer_bind 216..232
/label=KS primer
/note="common sequencing primer, one of multiple similar
variants"
CDS 287..2152
/codon_start=1
/label=AAV2-Rep
/translation="MPGFYEIVIKVPSDLDEHLPGISDSFVNWVAEKEWELPPDSDMDL
NLIEQAPLTVAEKLQRDFLTEWRRVSKAPEALFFVQFEKGESYFHMHVLVETTGVKSMV
LGRFLSQIREKLIQRIYRGIEPTLPNWFAVTKTRNGAGGGNKVVDECYIPNYLLPKTQP
ELQWAWTNMEQYLSACLNLTERKRLVAQHLTHVSQTQEQNKENQNPNSDAPVIRSKTSA
RYMELVGWLVDKGITSEKQWIQEDQASYISFNAASNSRSQIKAALDNAGKIMSLTKTAP
DYLVGQQPVEDISSNRIYKILELNGYDPQYAASVFLGWATKKFGKRNTIWLFGPATTGK
TNIAEAIAHTVPFYGCVNWTNENFPFNDCVDKMVIWWEEGKMTAKVVESAKAILGGSKV
RVDQKCKSSAQIDPTPVIVTSNTNMCAVIDGNSTTFEHQQPLQDRMFKFELTRRLDHDF
GKVTKQEVKDFFRWAKDHVVEVEHEFYVKKGGAKKRPAPSDADISEPKRVRESVAQPST
SDAEASINYADRYQNKCSRHVGMNLMLFPCRQCERLNQNSNICFTHGVKDCLECFPVSE
SQPVSVVKKAYQKLCYIHHIMGKVPDACTACDLVNVDLDDCVSEQ"
primer_bind 2074..2093
/label=AAV2-F
misc_feature 2169..4076
/label=AAV8-Cap
promoter complement(4689..4707)
/label=T3 promoter
/note="promoter for bacteriophage T3 RNA polymerase"
primer_bind complement(4727..4744)
/label=M13R（擎科常备）
primer_bind complement(4728..4744)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(4744..4764)
/label=M13-48(生工常备）
protein_bind 4752..4768
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4776..4806)
/label=lac promoter
/note="promoter for the E. coli lac operon"
primer_bind complement(4783..4802)
/label=lac-F
protein_bind 4821..4842
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(5131..5719)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 5522..5560
/label=ori-F
CDS complement(5890..6750)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6751..6855)
/gene="bla"
/label=AmpR promoter
primer_bind 6787..6806
/label=AmpR-R
rep_origin complement(6881..7336)
/direction=LEFT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
ORIGIN
1 cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
61 tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
121 ggttttccca gtcacgacgt tgtaaaacga cggccagtga gcgcgcgtaa tacgactcac
181 tatagggcga attgggtacc gggccccccc tcgatcgagg tcgacggtat cgggggagct
241 cgcagggtct ccattttgaa gcgggaggtt tgaacgcgca gccgccatgc cggggtttta
301 cgagattgtg attaaggtcc ccagcgacct tgacgagcat ctgcccggca tttctgacag
361 ctttgtgaac tgggtggccg agaaggaatg ggagttgccg ccagattctg acatggatct
421 gaatctgatt gagcaggcac ccctgaccgt ggccgagaag ctgcagcgcg actttctgac
481 ggaatggcgc cgtgtgagta aggccccgga ggctcttttc tttgtgcaat ttgagaaggg
541 agagagctac ttccacatgc acgtgctcgt ggaaaccacc ggggtgaaat ccatggtttt
601 gggacgtttc ctgagtcaga ttcgcgaaaa actgattcag agaatttacc gcgggatcga
661 gccgactttg ccaaactggt tcgcggtcac aaagaccaga aatggcgccg gaggcgggaa
721 caaggtggtg gatgagtgct acatccccaa ttacttgctc cccaaaaccc agcctgagct
781 ccagtgggcg tggactaata tggaacagta tttaagcgcc tgtttgaatc tcacggagcg
841 taaacggttg gtggcgcagc atctgacgca cgtgtcgcag acgcaggagc agaacaaaga
901 gaatcagaat cccaattctg atgcgccggt gatcagatca aaaacttcag ccaggtacat
961 ggagctggtc gggtggctcg tggacaaggg gattacctcg gagaagcagt ggatccagga
1021 ggaccaggcc tcatacatct ccttcaatgc ggcctccaac tcgcggtccc aaatcaaggc
1081 tgccttggac aatgcgggaa agattatgag cctgactaaa accgcccccg actacctggt
1141 gggccagcag cccgtggagg acatttccag caatcggatt tataaaattt tggaactaaa
1201 cgggtacgat ccccaatatg cggcttccgt ctttctggga tgggccacga aaaagttcgg
1261 caagaggaac accatctggc tgtttgggcc tgcaactacc gggaagacca acatcgcgga
1321 ggccatagcc cacactgtgc ccttctacgg gtgcgtaaac tggaccaatg agaactttcc
1381 cttcaacgac tgtgtcgaca agatggtgat ctggtgggag gaggggaaga tgaccgccaa
1441 ggtcgtggag tcggccaaag ccattctcgg aggaagcaag gtgcgcgtgg accagaaatg
1501 caagtcctcg gcccagatag acccgactcc cgtgatcgtc acctccaaca ccaacatgtg
1561 cgccgtgatt gacgggaact caacgacctt cgaacaccag cagccgttgc aagaccggat
1621 gttcaaattt gaactcaccc gccgtctgga tcatgacttt gggaaggtca ccaagcagga
1681 agtcaaagac tttttccggt gggcaaagga tcacgtggtt gaggtggagc atgaattcta
1741 cgtcaaaaag ggtggagcca agaaaagacc cgcccccagt gacgcagata taagtgagcc
1801 caaacgggtg cgcgagtcag ttgcgcagcc atcgacgtca gacgcggaag cttcgatcaa
1861 ctacgcggac aggtaccaaa acaaatgttc tcgtcacgtg ggcatgaatc tgatgctgtt
1921 tccctgcaga caatgcgaga gactgaatca gaattcaaat atctgcttca ctcacggtgt
1981 caaagactgt ttagagtgct ttcccgtgtc agaatctcaa cccgtttctg tcgtcaaaaa
2041 ggcgtatcag aaactgtgct acattcatca catcatggga aaggtgccag acgcttgcac
2101 tgcttgcgac ctggtcaatg tggacttgga tgactgtgtt tctgaacaat aaatgactta
2161 aaccaggtat ggctgccgat ggttatcttc cagattggct cgaggacaac ctctctgagg
2221 gcattcgcga gtggtgggcg ctgaaacctg gagccccgaa gcccaaagcc aaccagcaaa
2281 agcaggacga cggccggggt ctggtgcttc ctggctacaa gtacctcgga cccttcaacg
2341 gactcgacaa gggggagccc gtcaacgcgg cggacgcagc ggccctcgag cacgacaagg
2401 cctacgacca gcagctgcag gcgggtgaca atccgtacct gcggtataac cacgccgacg
2461 ccgagtttca ggagcgtctg caagaagata cgtcttttgg gggcaacctc gggcgagcag
2521 tcttccaggc caagaagcgg gttctcgaac ctctcggtct ggttgaggaa ggcgctaaga
2581 cggctcctgg aaagaagaga ccggtagagc catcacccca gcgttctcca gactcctcta
2641 cgggcatcgg caagaaaggc caacagcccg ccagaaaaag actcaatttt ggtcagactg
2701 gcgactcaga gtcagttcca gaccctcaac ctctcggaga acctccagca gcgccctctg
2761 gtgtgggacc taatacaatg gctgcaggcg gtggcgcacc aatggcagac aataacgaag
2821 gcgccgacgg agtgggtagt tcctcgggaa attggcattg cgattccaca tggctgggcg
2881 acagagtcat caccaccagc acccgaacct gggccctgcc cacctacaac aaccacctct
2941 acaagcaaat ctccaacggg acatcgggag gagccaccaa cgacaacacc tacttcggct
3001 acagcacccc ctgggggtat tttgacttta acagattcca ctgccacttt tcaccacgtg
3061 actggcagcg actcatcaac aacaactggg gattccggcc caagagactc agcttcaagc
3121 tcttcaacat ccaggtcaag gaggtcacgc agaatgaagg caccaagacc atcgccaata
3181 acctcaccag caccatccag gtgtttacgg actcggagta ccagctgccg tacgttctcg
3241 gctctgccca ccagggctgc ctgcctccgt tcccggcgga cgtgttcatg attccccagt
3301 acggctacct aacactcaac aacggtagtc aggccgtggg acgctcctcc ttctactgcc
3361 tggaatactt tccttcgcag atgctgagaa ccggcaacaa cttccagttt acttacacct
3421 tcgaggacgt gcctttccac agcagctacg cccacagcca gagcttggac cggctgatga
3481 atcctctgat tgaccagtac ctgtactact tgtctcggac tcaaacaaca ggaggcacgg
3541 caaatacgca gactctgggc ttcagccaag gtgggcctaa tacaatggcc aatcaggcaa
3601 agaactggct gccaggaccc tgttaccgcc aacaacgcgt ctcaacgaca accgggcaaa
3661 acaacaatag caactttgcc tggactgctg ggaccaaata ccatctgaat ggaagaaatt
3721 cattggctaa tcctggcatc gctatggcaa cacacaaaga cgacgaggag cgtttttttc
3781 ccagtaacgg gatcctgatt ttggcaaaca aaatgctgcc agagacaatg cggattacag
3841 cgatgtcatg ctcaccagcg aggaagaaat caaaaccact aaccctgtgg ctacagagga
3901 atacggtatc gtggcagata acttgcagca gcaaaacacg gctcctcaaa ttggaactgt
3961 caacagccag ggggccttac ccggtatggt ctggcagaac cgggacgtgt acctgcaggg
4021 tcccatctgg gccaagattc ctcacacgga cggcaacttc cacccgtctc cgctgatggg
4081 cggctttggc ctgaaacatc ctccgcctca gatcctgatc aagaacacgc ctgtacctgc
4141 ggatcctccg accaccttca accagtcaaa gctgaactct ttcatcacgc aatacagcac
4201 cggacaggtc agcgtggaaa ttgaatggga gctgcagaag gaaaacagca agcgctggaa
4261 ccccgagatc cagtacacct ccaactacta caaatctaca agtgtggact ttgctgttaa
4321 tacagaaggc gtgtactctg aaccccgccc cattggcacc cgttacctca cccgtaatct
4381 gtaattgcct gttaatcaat aaaccggttg attcgtttca gttgaacttt ggtctctgcg
4441 aagggcgaat tcgtttaaac ctgcaggact agaggtcctg tattagaggt cacgtgagtg
4501 ttttgcgaca ttttgcgaca ccatgtggtc acgctgggta tttaagcccg agtgagcacg
4561 cagggtctcc attttgaagc gggaggtttg aacgcgcagc cgccaagccg aattctgcag
4621 atatccatca cactggcggc cgctcgacta gagcggccgc caccgcggtg gagctccagc
4681 ttttgttccc tttagtgagg gttaattgcg cgcttggcgt aatcatggtc atagctgttt
4741 cctgtgtgaa attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag
4801 tgtaaagcct ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg
4861 cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg
4921 gggagaggcg gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc
4981 tcggtcgttc ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc
5041 acagaatcag gggataacgg caggaaagaa catgtgagca aaaggccagc aaaaggccag
5101 gaaccgtaaa aaggccgcgt tgctggcgtt tttccatagg ctccgccccc ctgacgagca
5161 tcacaaaaat cgacgctcaa gtcagaggtg gcgaaacccg acaggactat aaagatacca
5221 ggcgtttccc cctggaagct ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg
5281 atacctgtcc gcctttctcc cttcgggaag cgtggcgctt tctcatagct cacgctgtag
5341 gtatctcagt tcggtgtagg tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt
5401 tcagcccgac cgctgcgcct tatccggtaa ctatcgtctt gagtccaacc cggtaagaca
5461 cgacttatcg ccactggcag cagccactgg taacaggatt agcagagcga ggtatgtagg
5521 cggtgctaca gagttcttga agtggtggcc taactacggc tacactagaa gaacagtatt
5581 tggtatctgc gctctgctga agccagttac cttcggaaaa agagttggta gctcttgatc
5641 cggcaaacaa accaccgctg gtagcggtgg tttttttgtt tgcaagcagc agattacgcg
5701 cagaaaaaaa ggatctcaag aagatccttt gatcttttct acggggtctg acgctcagtg
5761 gaacgaaaac tcacgttaag ggattttggt catgagatta tcaaaaagga tcttcaccta
5821 gatcctttta aattaaaaat gaagttttaa atcaatctaa agtatatatg agtaaacttg
5881 gtctgacagt taccaatgct taatcagtga ggcacctatc tcagcgatct gtctatttcg
5941 ttcatccata gttgcctgac tccccgtcgt gtagataact acgatacggg agggcttacc
6001 atctggcccc agtgctgcaa tgataccgcg agacccacgc tcaccggctc cagatttatc
6061 agcaataaac cagccagccg gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc
6121 ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
6181 tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
6241 ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
6301 caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
6361 gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
6421 atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
6481 accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
6541 aaaagtgctc atcattggaa aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct
6601 gttgagatcc agttcgatgt aacccactcg tgcacccaac tgatcttcag catcttttac
6661 tttcaccagc gtttctgggt gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat
6721 aagggcgaca cggaaatgtt gaatactcat actcttcctt tttcaatatt attgaagcat
6781 ttatcagggt tattgtctca tgagcggata catatttgaa tgtatttaga aaaataaaca
6841 aataggggtt ccgcgcacat ttccccgaaa agtgccacct aaattgtaag cgttaatatt
6901 ttgttaaaat tcgcgttaaa tttttgttaa atcagctcat tttttaacca ataggccgaa
6961 atcggcaaaa tcccttataa atcaaaagaa tagaccgaga tagggttgag tgttgttcca
7021 gtttggaaca agagtccact attaaagaac gtggactcca acgtcaaagg gcgaaaaacc
7081 gtctatcagg gcgatggccc actacgtgaa ccatcaccct aatcaagttt tttggggtcg
7141 aggtgccgta aagcactaaa tcggaaccct aaagggagcc cccgatttag agcttgacgg
7201 ggaaagccgg cgaacgtggc gagaaaggaa gggaagaaag cgaaaggagc gggcgctagg
7261 gcgctggcaa gtgtagcggt cacgctgcgc gtaaccacca cacccgccgc gcttaatgcg
7321 ccgctacagg gcgcgt
//
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