| 质粒名称: | pU6-Decoy |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 80324 |
| 存储实验室: | Sebastian Lourido |
|
载体骨架基本信息 |
|
| 载体名称: | pU6-Universal |
| 载体抗性: | 氨苄青霉素 |
| 空载体大小: | 8360 bp |
| 完整质粒大小: | 8380 bp |
| 载体修饰: | 诱饵原间隔区已被克隆到BsaI位点。 |
| 载体类型: | CRISPR |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | Decoy sgRNA |
| 启动子 | U6 |
| 克隆方法: | Gibson克隆 |
| 5' 测序引物 | GCCCATGGGATGAGACAAAG |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pU6-Decoy was a gift from Sebastian Lourido (Addgene plasmid # 80324 ; http://n2t.net/addgene:80324 ; RRID:Addgene_80324) |
| 参考文献部分: | A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential Apicomplexan Genes. Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P, Saeij JP, Carruthers VB, Niles JC, Lourido S. Cell. 2016 Sep 8;166(6):1423-1435.e12. doi: 10.1016/j.cell.2016.08.019. Epub 2016 Sep 2. 10.1016/j.cell.2016.08.019 PubMed 27594426 |
买家导航
pU6-Decoy载体 pU6Decoy质粒基本信息
pU6-Decoy质粒图谱 pU6Decoy载体图谱和pU6-Decoy载体序列 pU6Decoy质粒序列多克隆位点信息
pU6-Decoy质粒 pU6Decoy载体简介
pU6-Decoy质粒序列 pU6Decoy载体序列
LOCUS pU6-Decoy 8380 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Encodes Cas9 and a CRISPR sgRNA that alleviates toxicity to Cas9 in
Toxoplasma gondii.
ACCESSION .
VERSION .
KEYWORDS pU6-Decoy
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8380)
AUTHORS Sidik SM, Huet D, Ganesan SM, Huynh MH, Wang T, Nasamu AS, Thiru P,
Saeij JP, Carruthers VB, Niles JC, Lourido S
TITLE A Genome-wide CRISPR Screen in Toxoplasma Identifies Essential
Apicomplexan Genes.
JOURNAL Cell. 2016 Sep 8;166(6):1423-1435.e12. doi:
10.1016/j.cell.2016.08.019. Epub 2016 Sep 2.
PUBMED 27594426
REFERENCE 2 (bases 1 to 8380)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..8380
/organism="synthetic DNA construct"
/mol_type="other DNA"
CDS 514..579
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
CDS 586..606
/codon_start=1
/product="nuclear localization signal of SV40 (simian virus
40) large T antigen"
/label=SV40 NLS
/translation="PKKKRKV"
CDS 631..4731
/codon_start=1
/product="Cas9 (Csn1) endonuclease from the Streptococcus
pyogenes Type II CRISPR/Cas system"
/label=Cas9
/note="generates RNA-guided double strand breaks in DNA"
/translation="DKKYSIGLDIGTNSVGWAVITDEYKVPSKKFKVLGNTDRHSIKKN
LIGALLFDSGETAEATRLKRTARRRYTRRKNRICYLQEIFSNEMAKVDDSFFHRLEESF
LVEEDKKHERHPIFGNIVDEVAYHEKYPTIYHLRKKLVDSTDKADLRLIYLALAHMIKF
RGHFLIEGDLNPDNSDVDKLFIQLVQTYNQLFEENPINASGVDAKAILSARLSKSRRLE
NLIAQLPGEKKNGLFGNLIALSLGLTPNFKSNFDLAEDAKLQLSKDTYDDDLDNLLAQI
GDQYADLFLAAKNLSDAILLSDILRVNTEITKAPLSASMIKRYDEHHQDLTLLKALVRQ
QLPEKYKEIFFDQSKNGYAGYIDGGASQEEFYKFIKPILEKMDGTEELLVKLNREDLLR
KQRTFDNGSIPHQIHLGELHAILRRQEDFYPFLKDNREKIEKILTFRIPYYVGPLARGN
SRFAWMTRKSEETITPWNFEEVVDKGASAQSFIERMTNFDKNLPNEKVLPKHSLLYEYF
TVYNELTKVKYVTEGMRKPAFLSGEQKKAIVDLLFKTNRKVTVKQLKEDYFKKIECFDS
VEISGVEDRFNASLGTYHDLLKIIKDKDFLDNEENEDILEDIVLTLTLFEDREMIEERL
KTYAHLFDDKVMKQLKRRRYTGWGRLSRKLINGIRDKQSGKTILDFLKSDGFANRNFMQ
LIHDDSLTFKEDIQKAQVSGQGDSLHEHIANLAGSPAIKKGILQTVKVVDELVKVMGRH
KPENIVIEMARENQTTQKGQKNSRERMKRIEEGIKELGSQILKEHPVENTQLQNEKLYL
YYLQNGRDMYVDQELDINRLSDYDVDHIVPQSFLKDDSIDNKVLTRSDKNRGKSDNVPS
EEVVKKMKNYWRQLLNAKLITQRKFDNLTKAERGGLSELDKAGFIKRQLVETRQITKHV
AQILDSRMNTKYDENDKLIREVKVITLKSKLVSDFRKDFQFYKVREINNYHHAHDAYLN
AVVGTALIKKYPKLESEFVYGDYKVYDVRKMIAKSEQEIGKATAKYFFYSNIMNFFKTE
ITLANGEIRKRPLIETNGETGEIVWDKGRDFATVRKVLSMPQVNIVKKTEVQTGGFSKE
SILPKRNSDKLIARKKDWDPKKYGGFDSPTVAYSVLVVAKVEKGKSKKLKSVKELLGIT
IMERSSFEKNPIDFLEAKGYKEVKKDLIIKLPKYSLFELENGRKRMLASAGELQKGNEL
ALPSKYVNFLYLASHYEKLKGSPEDNEQKQLFVEQHKHYLDEIIEQISEFSKRVILADA
NLDKVLSAYNKHRDKPIREQAENIIHLFTLTNLGAPAAFKYFDTTIDRKRYTSTKEVLD
ATLIHQSITGLYETRIDLSQLGGD"
CDS 4732..4779
/codon_start=1
/product="bipartite nuclear localization signal from
nucleoplasmin"
/label=nucleoplasmin NLS
/translation="KRPAATKKAGQAKKKK"
primer_bind complement(4807..4824)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 4813..5020
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
repeat_region 5029..5169
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
repeat_region 5029..5158
/label=AAV2 ITR
rep_origin 5244..5699
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
primer_bind complement(5331..5350)
/label=F1ori-R
/note="F1 origin, reverse primer"
primer_bind 5541..5562
/label=F1ori-F
/note="F1 origin, forward primer"
primer_bind complement(5716..5735)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 5835..5857
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(5895..5913)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 5981..6085
/gene="bla"
/label=AmpR promoter
CDS 6086..6946
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6304..6323)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7117..7705
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 7606..7625
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
misc_RNA 8288..8363
/label=gRNA scaffold
/note="guide RNA scaffold for the Streptococcus pyogenes
CRISPR/Cas9 system"
ORIGIN
1 ctagaggtac catgcatgtc ccgcgttcgt gaaattctct gcatcagcgg agtgatcagg
61 aatcatcgtc tcagcgggat gacgttgcgg agcaggccgg ctcgcggtgg gcagtcagat
121 gccgaaggcg taactcagga cggcttgcgc tcatcgcaga acaggggtgg tgcctgcatt
181 gggtgcggtt ggtgatcctg gttggaccgg tggagatgcg cgcgcacgaa ggggatgtgt
241 cagaaacatt ttgtttgttc tctgtgaact tttagatgtg ttaaaggcgg cgaatattag
301 cagagagtcc tccttgttgg attctctctt gaatttcgcc ctttccttct ctttgcgagt
361 ctcgtagaga acaagcactc gttcgccgtc cctgacgacg caacccgcgc agaagacatc
421 caccaaacgg tgttacacaa tcaccttgtg tgaagttctt gcggaaaact actcgttggc
481 attttttctt gaattccctt tttcgacacc atggactata aggaccacga cggagactac
541 aaggatcatg atattgatta caaagacgat gacgataaga tggccccaaa gaagaagcgg
601 aaggtcggta tccacggagt cccagcagcc gacaagaagt acagcatcgg cctggacatc
661 ggcaccaact ctgtgggctg ggccgtgatc accgacgagt acaaggtgcc cagcaagaaa
721 ttcaaggtgc tgggcaacac cgaccggcac agcatcaaga agaacctgat cggagccctg
781 ctgttcgaca gcggcgaaac agccgaggcc acccggctga agagaaccgc cagaagaaga
841 tacaccagac ggaagaaccg gatctgctat ctgcaagaga tcttcagcaa cgagatggcc
901 aaggtggacg acagcttctt ccacagactg gaagagtcct tcctggtgga agaggataag
961 aagcacgagc ggcaccccat cttcggcaac atcgtggacg aggtggccta ccacgagaag
1021 taccccacca tctaccacct gagaaagaaa ctggtggaca gcaccgacaa ggccgacctg
1081 cggctgatct atctggccct ggcccacatg atcaagttcc ggggccactt cctgatcgag
1141 ggcgacctga accccgacaa cagcgacgtg gacaagctgt tcatccagct ggtgcagacc
1201 tacaaccagc tgttcgagga aaaccccatc aacgccagcg gcgtggacgc caaggccatc
1261 ctgtctgcca gactgagcaa gagcagacgg ctggaaaatc tgatcgccca gctgcccggc
1321 gagaagaaga atggcctgtt cggaaacctg attgccctga gcctgggcct gacccccaac
1381 ttcaagagca acttcgacct ggccgaggat gccaaactgc agctgagcaa ggacacctac
1441 gacgacgacc tggacaacct gctggcccag atcggcgacc agtacgccga cctgtttctg
1501 gccgccaaga acctgtccga cgccatcctg ctgagcgaca tcctgagagt gaacaccgag
1561 atcaccaagg cccccctgag cgcctctatg atcaagagat acgacgagca ccaccaggac
1621 ctgaccctgc tgaaagctct cgtgcggcag cagctgcctg agaagtacaa agagattttc
1681 ttcgaccaga gcaagaacgg ctacgccggc tacattgacg gcggagccag ccaggaagag
1741 ttctacaagt tcatcaagcc catcctggaa aagatggacg gcaccgagga actgctcgtg
1801 aagctgaaca gagaggacct gctgcggaag cagcggacct tcgacaacgg cagcatcccc
1861 caccagatcc acctgggaga gctgcacgcc attctgcggc ggcaggaaga tttttaccca
1921 ttcctgaagg acaaccggga aaagatcgag aagatcctga ccttccgcat cccctactac
1981 gtgggccctc tggccagggg aaacagcaga ttcgcctgga tgaccagaaa gagcgaggaa
2041 accatcaccc cctggaactt cgaggaagtg gtggacaagg gcgcttccgc ccagagcttc
2101 atcgagcgga tgaccaactt cgataagaac ctgcccaacg agaaggtgct gcccaagcac
2161 agcctgctgt acgagtactt caccgtgtat aacgagctga ccaaagtgaa atacgtgacc
2221 gagggaatga gaaagcccgc cttcctgagc ggcgagcaga aaaaggccat cgtggacctg
2281 ctgttcaaga ccaaccggaa agtgaccgtg aagcagctga aagaggacta cttcaagaaa
2341 atcgagtgct tcgactccgt ggaaatctcc ggcgtggaag atcggttcaa cgcctccctg
2401 ggcacatacc acgatctgct gaaaattatc aaggacaagg acttcctgga caatgaggaa
2461 aacgaggaca ttctggaaga tatcgtgctg accctgacac tgtttgagga cagagagatg
2521 atcgaggaac ggctgaaaac ctatgcccac ctgttcgacg acaaagtgat gaagcagctg
2581 aagcggcgga gatacaccgg ctggggcagg ctgagccgga agctgatcaa cggcatccgg
2641 gacaagcagt ccggcaagac aatcctggat ttcctgaagt ccgacggctt cgccaacaga
2701 aacttcatgc agctgatcca cgacgacagc ctgaccttta aagaggacat ccagaaagcc
2761 caggtgtccg gccagggcga tagcctgcac gagcacattg ccaatctggc cggcagcccc
2821 gccattaaga agggcatcct gcagacagtg aaggtggtgg acgagctcgt gaaagtgatg
2881 ggccggcaca agcccgagaa catcgtgatc gaaatggcca gagagaacca gaccacccag
2941 aagggacaga agaacagccg cgagagaatg aagcggatcg aagagggcat caaagagctg
3001 ggcagccaga tcctgaaaga acaccccgtg gaaaacaccc agctgcagaa cgagaagctg
3061 tacctgtact acctgcagaa tgggcgggat atgtacgtgg accaggaact ggacatcaac
3121 cggctgtccg actacgatgt ggaccatatc gtgcctcaga gctttctgaa ggacgactcc
3181 atcgacaaca aggtgctgac cagaagcgac aagaaccggg gcaagagcga caacgtgccc
3241 tccgaagagg tcgtgaagaa gatgaagaac tactggcggc agctgctgaa cgccaagctg
3301 attacccaga gaaagttcga caatctgacc aaggccgaga gaggcggcct gagcgaactg
3361 gataaggccg gcttcatcaa gagacagctg gtggaaaccc ggcagatcac aaagcacgtg
3421 gcacagatcc tggactcccg gatgaacact aagtacgacg agaatgacaa gctgatccgg
3481 gaagtgaaag tgatcaccct gaagtccaag ctggtgtccg atttccggaa ggatttccag
3541 ttttacaaag tgcgcgagat caacaactac caccacgccc acgacgccta cctgaacgcc
3601 gtcgtgggaa ccgccctgat caaaaagtac cctaagctgg aaagcgagtt cgtgtacggc
3661 gactacaagg tgtacgacgt gcggaagatg atcgccaaga gcgagcagga aatcggcaag
3721 gctaccgcca agtacttctt ctacagcaac atcatgaact ttttcaagac cgagattacc
3781 ctggccaacg gcgagatccg gaagcggcct ctgatcgaga caaacggcga aaccggggag
3841 atcgtgtggg ataagggccg ggattttgcc accgtgcgga aagtgctgag catgccccaa
3901 gtgaatatcg tgaaaaagac cgaggtgcag acaggcggct tcagcaaaga gtctatcctg
3961 cccaagagga acagcgataa gctgatcgcc agaaagaagg actgggaccc taagaagtac
4021 ggcggcttcg acagccccac cgtggcctat tctgtgctgg tggtggccaa agtggaaaag
4081 ggcaagtcca agaaactgaa gagtgtgaaa gagctgctgg ggatcaccat catggaaaga
4141 agcagcttcg agaagaatcc catcgacttt ctggaagcca agggctacaa agaagtgaaa
4201 aaggacctga tcatcaagct gcctaagtac tccctgttcg agctggaaaa cggccggaag
4261 agaatgctgg cctctgccgg cgaactgcag aagggaaacg aactggccct gccctccaaa
4321 tatgtgaact tcctgtacct ggccagccac tatgagaagc tgaagggctc ccccgaggat
4381 aatgagcaga aacagctgtt tgtggaacag cacaagcact acctggacga gatcatcgag
4441 cagatcagcg agttctccaa gagagtgatc ctggccgacg ctaatctgga caaagtgctg
4501 tccgcctaca acaagcaccg ggataagccc atcagagagc aggccgagaa tatcatccac
4561 ctgtttaccc tgaccaatct gggagcccct gccgccttca agtactttga caccaccatc
4621 gaccggaaga ggtacaccag caccaaagag gtgctggacg ccaccctgat ccaccagagc
4681 atcaccggcc tgtacgagac acggatcgac ctgtctcagc tgggaggcga caaaaggccg
4741 gcggccacga aaaaggccgg ccaggcaaaa aagaaaaagt aagaattcct agagctcgct
4801 gatcagcctc gactgtgcct tctagttgcc agccatctgt tgtttgcccc tcccccgtgc
4861 cttccttgac cctggaaggt gccactccca ctgtcctttc ctaataaaat gaggaaattg
4921 catcgcattg tctgagtagg tgtcattcta ttctgggggg tggggtgggg caggacagca
4981 agggggagga ttgggaagag aatagcaggc atgctgggga gcggccgcag gaacccctag
5041 tgatggagtt ggccactccc tctctgcgcg ctcgctcgct cactgaggcc gggcgaccaa
5101 aggtcgcccg acgcccgggc tttgcccggg cggcctcagt gagcgagcga gcgcgcagct
5161 gcctgcaggg gcgcctgatg cggtattttc tccttacgca tctgtgcggt atttcacacc
5221 gcatacgtca aagcaaccat agtacgcgcc ctgtagcggc gcattaagcg cggcgggtgt
5281 ggtggttacg cgcagcgtga ccgctacact tgccagcgcc ctagcgcccg ctcctttcgc
5341 tttcttccct tcctttctcg ccacgttcgc cggctttccc cgtcaagctc taaatcgggg
5401 gctcccttta gggttccgat ttagtgcttt acggcacctc gaccccaaaa aacttgattt
5461 gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg gtttttcgcc ctttgacgtt
5521 ggagtccacg ttctttaata gtggactctt gttccaaact ggaacaacac tcaaccctat
5581 ctcgggctat tcttttgatt tataagggat tttgccgatt tcggcctatt ggttaaaaaa
5641 tgagctgatt taacaaaaat ttaacgcgaa ttttaacaaa atattaacgt ttacaatttt
5701 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagc cccgacaccc
5761 gccaacaccc gctgacgcgc cctgacgggc ttgtctgctc ccggcatccg cttacagaca
5821 agctgtgacc gtctccggga gctgcatgtg tcagaggttt tcaccgtcat caccgaaacg
5881 cgcgagacga aagggcctcg tgatacgcct atttttatag gttaatgtca tgataataat
5941 ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc ctatttgttt
6001 atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct gataaatgct
6061 tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg cccttattcc
6121 cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg tgaaagtaaa
6181 agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc tcaacagcgg
6241 taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca cttttaaagt
6301 tctgctatgt ggcgcggtat tatcccgtat tgacgccggg caagagcaac tcggtcgccg
6361 catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa agcatcttac
6421 ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg ataacactgc
6481 ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt ttttgcacaa
6541 catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg aagccatacc
6601 aaacgacgag cgtgacacca cgatgcctgt agcaatggca acaacgttgc gcaaactatt
6661 aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga tggaggcgga
6721 taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta ttgctgataa
6781 atctggagcc ggtgagcgtg gaagccgcgg tatcattgca gcactggggc cagatggtaa
6841 gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg atgaacgaaa
6901 tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt cagaccaagt
6961 ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa ggatctaggt
7021 gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt cgttccactg
7081 agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt ttctgcgcgt
7141 aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt tgccggatca
7201 agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga taccaaatac
7261 tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag caccgcctac
7321 atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata agtcgtgtct
7381 taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg gctgaacggg
7441 gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga gatacctaca
7501 gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca ggtatccggt
7561 aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa acgcctggta
7621 tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt tgtgatgctc
7681 gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac ggttcctggc
7741 cttttgctgg ccttttgctc acatgggatg agacaaagtg cgcgagttga aatcgtcgtg
7801 gggacgattt caccgcggcc acatgttgga gacactgagg gcacacggga aacgcgaaag
7861 atttcaaatt aacgtaccca aacgcgaaag cttgcgcagc atacactcga agcgaacatc
7921 ccgaaccatc gagaggcgga gagcgataag tctttcacgc tgcgaagtgt tgcgacggct
7981 gcgccgctgc actgtgaatt gggcgccaat attgcatcct aggcctgacg cgcctcctgc
8041 agaacgcgag acactgggat atgtagagcc aagggggaaa ccttcgaact ctcgaatgtc
8101 ttctctgaca agaatcatat ttccatcagt tctgtcagat tttcaaatgg cgacctgcag
8161 aggcctgctt cctccctgtg cgctcttcga aggggctttc tgtcgcgcag ggtcacctcg
8221 tccccgaagg gggtgtttgc cttctggtaa atggggatgt caagttgaga atgcagttta
8281 gcaccgtgtt ttagagctag aaatagcaag ttaaaataag gctagtccgt tatcaacttg
8341 aaaaagtggc accgagtcgg tgcttttttt ttctttttct
//
