| 质粒名称: | pCDH-LplA-mEGFP |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002264 |
| 产品用途: | Expression of LplA-mEGFP protein in U2OS cells by lentiviral transduction |
| 存储实验室: | Anping ZENG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pCDH-CMV |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | lplA-mEGFP |
| 物种来源: | E. coli |
| 密码子优化: | 哺乳细胞表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
A globular protein exhibits rare phase behavior and forms chemically regulated orthogonal condensates in cells
Jinglei Nie, Xinyi Zhang, Zhijuan Hu, Wei Wang, Martin A. Schroer, Jie Ren, Dmitri Svergun, Anyang Chen, Peiguo Yang, An-Ping Zeng
Nature Communications, 16, 2025-03-12
|
pCDH-LplA-mEGFP
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pCDH-LplA-mEGFP载体质粒基本信息
pCDH-LplA-mEGFP质粒图谱载体图谱和pCDH-LplA-mEGFP载体序列质粒序列多克隆位点信息
pCDH-LplA-mEGFP质粒载体简介
pCDH-LplA-mEGFP质粒序列载体序列
LOCUS pCDH-LplA-mEGFP 8184 bp DNA circular SYN 09-MAY-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 8184)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Aug 24, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..8184
/mol_type="other DNA"
/organism="synthetic DNA construct"
misc_feature 409..525
/label=cPPT/CTS
/note="central polypurine tract and central termination
sequence of HIV-1"
enhancer 548..851
/label=CMV enhancer
/note="human cytomegalovirus immediate early enhancer"
promoter 852..1055
/label=CMV promoter
/note="human cytomegalovirus (CMV) immediate early
promoter"
CDS 2144..2860
/codon_start=1
/product="enhanced GFP with monomerizing A206K mutation
(Zacharias et al., 2002)"
/label=mEGFP
/note="mammalian codon-optimized"
/translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
FVTAAGITLGMDELYK"
misc_feature 2867..3454
/label=WPRE
/note="woodchuck hepatitis virus posttranscriptional
regulatory element"
LTR 3527..3760
/label=3' LTR (Delta-U3)
/note="self-inactivating 3' long terminal repeat (LTR) from
HIV-1"
polyA_signal 3832..3953
/label=SV40 poly(A) signal
/note="SV40 polyadenylation signal"
rep_origin 3993..4128
/label=SV40 ori
/note="SV40 origin of replication"
primer_bind complement(4161..4177)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
protein_bind 4185..4201
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(4209..4239)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4254..4275
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
rep_origin complement(4563..5151)
/direction=LEFT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5322..6182)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(6183..6287)
/gene="bla"
/label=AmpR promoter
primer_bind 6761..6777
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 6792..7018
/label=RSV promoter
/note="Rous sarcoma virus enhancer/promoter"
LTR 7019..7199
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from HIV-1"
misc_feature 7246..7371
/label=HIV-1 Psi
/note="packaging signal of human immunodeficiency virus
type 1"
misc_feature 7864..8097
/label=RRE
/note="The Rev response element (RRE) of HIV-1 allows for
Rev-dependent mRNA export from the nucleus to the
cytoplasm."
ORIGIN
1 ttggaatcac acgacctgga tggagtggga cagagaaatt aacaattaca caagcttaat
61 acactcctta attgaagaat cgcaaaacca gcaagaaaag aatgaacaag aattattgga
121 attagataaa tgggcaagtt tgtggaattg gtttaacata acaaattggc tgtggtatat
181 aaaattattc ataatgatag taggaggctt ggtaggttta agaatagttt ttgctgtact
241 ttctatagtg aatagagtta ggcagggata ttcaccatta tcgtttcaga cccacctccc
301 aaccccgagg ggacccgaca ggcccgaagg aatagaagaa gaaggtggag agagagacag
361 agacagatcc attcgattag tgaacggatc tcgacggtat cggttaactt ttaaaagaaa
421 aggggggatt ggggggtaca gtgcagggga aagaatagta gacataatag caacagacat
481 acaaactaaa gaattacaaa aacaaattac aaaattcaaa attttatcga tactagttgg
541 agttccgcgt tacataactt acggtaaatg gcccgcctgg ctgaccgccc aacgaccccc
601 gcccattgac gtcaataatg acgtatgttc ccatagtaac gccaataggg actttccatt
661 gacgtcaatg ggtggagtat ttacggtaaa ctgcccactt ggcagtacat caagtgtatc
721 atatgccaag tacgccccct attgacgtca atgacggtaa atggcccgcc tggcattatg
781 cccagtacat gaccttatgg gactttccta cttggcagta catctacgta ttagtcatcg
841 ctattaccat ggtgatgcgg ttttggcagt acatcaatgg gcgtggatag cggtttgact
901 cacggggatt tccaagtctc caccccattg acgtcaatgg gagtttgttt tggcaccaaa
961 atcaacggga ctttccaaaa tgtcgtaaca actccgcccc attgacgcaa atgggcggta
1021 ggcgtgtacg gtgggaggtc tatataagca gagctggttt agtgaaccgt cagattctag
1081 accgctagcg ctaccggtcg ccaccatgtc cacattacgc ctgctcatct ctgactctta
1141 cgacccgtgg tttaacctgg cggtggaaga gtgtattttt cgccaaatgc ccgccacgca
1201 gcgcgttctg tttctctggc gcaatgccga cacggtagta attggtcgcg cgcagaaccc
1261 gtggaaagag tgtaataccc ggcggatgga agaagataac gtccgcctgg cgcggcgcag
1321 tagcggtggc ggcgcggtgt tccacgatct cggcaatacc tgctttacct ttatggctgg
1381 caagccggag tacgataaaa ctatctccac gtcgattgtg ctcaatgcgc tgaacgcgct
1441 cggcgtcagc gccgaagcgt ccggacgtaa cgatctggtg gtgaaaaccg tcgaaggcga
1501 ccgcaaagtc tcaggctcgg cctatcgcga aaccaaagat cgcggcttcc accacggcac
1561 cttgctactc aatgccgacc tcagccgcct ggcaaactat ctcaatccgg ataaaaagaa
1621 actggcggcg aaaggcatta cgtcggtacg ttcccgcgtg accaacctca ccgagctgtt
1681 gccggggatc acccatgagc aggtttgcga ggccataacc gaggcctttt tcgcccatta
1741 tggcgagcgc gtggaagcgg aaatcatctc cccgaacaaa acgccagact tgccaaactt
1801 cgccgaaacc tttgcccgcc agagtagctg ggaatggaac ttcggtcagg ctccggcatt
1861 ctcgcatctg ctggatgaac gctttacctg gggcggcgtg gaactgcatt tcgacgttga
1921 aaaaggccat atcacccgcg cccaggtgtt taccgacagc ctcaaccccg cgccgctgga
1981 agccctcgcc ggacgactgc aaggctgcct gtaccgcgca gatatgctgc aacaggagtg
2041 cgaagcgctg ttggttgact tcccggaaca ggaaaaagag ctacgggagt tatcggcatg
2101 gatggcgggg gctgtaaggg gtggaggctc aggtggtggt agcgtgagca agggcgagga
2161 gctgttcacc ggggtggtgc ccatcctggt cgagctggac ggcgacgtaa acggccacaa
2221 gttcagcgtg tccggcgagg gcgagggcga tgccacctac ggcaagctga ccctgaagtt
2281 catctgcacc accggcaagc tgcccgtgcc ctggcccacc ctcgtgacca ccctgaccta
2341 cggcgtgcag tgcttcagcc gctaccccga ccacatgaag cagcacgact tcttcaagtc
2401 cgccatgccc gaaggctacg tccaggagcg caccatcttc ttcaaggacg acggcaacta
2461 caagacccgc gccgaggtga agttcgaggg cgacaccctg gtgaaccgca tcgagctgaa
2521 gggcatcgac ttcaaggagg acggcaacat cctggggcac aagctggagt acaactacaa
2581 cagccacaac gtctatatca tggccgacaa gcagaagaac ggcatcaagg tgaacttcaa
2641 gatccgccac aacatcgagg acggcagcgt gcagctcgcc gaccactacc agcagaacac
2701 ccccatcggc gacggccccg tgctgctgcc cgacaaccac tacctgagca cccagtccaa
2761 gctgagcaaa gaccccaacg agaagcgcga tcacatggtc ctgctggagt tcgtgaccgc
2821 cgccgggatc actctcggca tggacgagct gtacaagtag ggatccaatc aacctctgga
2881 ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt ttacgctatg
2941 tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg ctttcatttt
3001 ctcctccttg tataaatcct ggttgctgtc tctttatgag gagttgtggc ccgttgtcag
3061 gcaacgtggc gtggtgtgca ctgtgtttgc tgacgcaacc cccactggtt ggggcattgc
3121 caccacctgt cagctccttt ccgggacttt cgctttcccc ctccctattg ccacggcgga
3181 actcatcgcc gcctgccttg cccgctgctg gacaggggct cggctgttgg gcactgacaa
3241 ttccgtggtg ttgtcgggga aatcatcgtc ctttccttgg ctgctcgcct gtgttgccac
3301 ctggattctg cgcgggacgt ccttctgcta cgtcccttcg gccctcaatc cagcggacct
3361 tccttcccgc ggcctgctgc cggctctgcg gcctcttccg cgtctccgcc ttcgccctca
3421 gacgagtcgg atctcccttt ggccgcctcc ccgccggtac ctttaagacc aatgacttac
3481 aaggcagctg tagatcttag ccacttttta aaagaaaagg ggggactgga agggctaatt
3541 cactcccaac gaagataaga tctgcttttt gcttgtactg ggtctctctg gttagaccag
3601 atctgagcct gggagctctc tggctaacta gggaacccac tgcttaagcc tcaataaagc
3661 ttgccttgag tgcttcaagt agtgtgtgcc cgtctgttgt gtgactctgg taactagaga
3721 tccctcagac ccttttagtc agtgtggaaa atctctagca gtagtagttc atgtcatctt
3781 attattcagt atttataact tgcaaagaaa tgaatatcag agagtgagag gaacttgttt
3841 attgcagctt ataatggtta caaataaagc aatagcatca caaatttcac aaataaagca
3901 tttttttcac tgcattctag ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc
3961 tggctctagc tatcccgccc ctaactccgc ccatcccgcc cctaactccg cccagttccg
4021 cccattctcc gccccatggc tgactaattt tttttattta tgcagaggcc gaggccgcct
4081 cggcctctga gctattccag aagtagtgag gaggcttttt tggaggccta gacttttgca
4141 gagaccaaat tcgtaatcat gtcatagctg tttcctgtgt gaaattgtta tccgctcaca
4201 attccacaca acatacgagc cggaagcata aagtgtaaag cctggggtgc ctaatgagtg
4261 agctaactca cattaattgc gttgcgctca ctgcccgctt tccagtcggg aaacctgtcg
4321 tgccagctgc attaatgaat cggccaacgc gcggggagag gcggtttgcg tattgggcgc
4381 tcttccgctt cctcgctcac tgactcgctg cgctcggtcg ttcggctgcg gcgagcggta
4441 tcagctcact caaaggcggt aatacggtta tccacagaat caggggataa cgcaggaaag
4501 aacatgtgag caaaaggcca gcaaaaggcc aggaacctta aaaaggccgc gttgctggcg
4561 tttttccata ggctccgccc ccctgacgag catcacaaaa atcgacgctc aagtcagagg
4621 tggcgaaacc cgacaggact ataaagatac caggcgtttc cccctggaag ctccctcgtg
4681 cgctctcctg ttccgaccct gccgcttacc ggatacctgt ccgcctttct cccttcggga
4741 agcgtggcgc tttctcatag ctcacgctgt aggtatctca gttcggtgta ggtcgttcgc
4801 tccaagctgg gctgtgtgca cgaacccccc gttcagcccg accgctgcgc cttatccggt
4861 aactatcgtc ttgagtccaa cccggtaaga cacgacttat cgccactggc agcagccact
4921 ggtaacagga ttagcagagc gaggtatgta ggcggtgcta cagagttctt gaagtggtgg
4981 cctaactacg gctacactag aagaacagta tttggtatct gcgctctgct gaagccagtt
5041 accttcggaa aaagagttgg tagctcttga tccggcaaac aaaccaccgc tggtagcggt
5101 ggtttttttg tttgcaagca gcagattacg cgcagaaaaa aaggatctca agaagatcct
5161 ttgatctttt ctacggggtc tgacgctcag tggaacgaaa actcacgtta agggattttg
5221 gtcatgagat tatcaaaaag gatcttcacc tagatccttt taaattaaaa atgaagtttt
5281 aaatcaatct aaagtatata tgagtaaact tggtctgaca gttaccaatg cttaatcagt
5341 gaggcaccta tctcagcgat ctgtctattt cgttcatcca tagttgcctg actccccgtc
5401 gtgtagataa ctacgatacg ggagggctta ccatctggcc ccagtgctgc aatgataccg
5461 cgagacccac gctcaccggc tccagattta tcagcaataa accagccagc cggaagggcc
5521 gagcgcagaa gtggtcctgc aactttatcc gcctccatcc agtctattaa ttgttgccgg
5581 gaagctagag taagtagttc gccagttaat agtttgcgca acgttgttgc cattgctaca
5641 ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat tcagctccgg ttcccaacga
5701 tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag cggttagctc cttcggtcct
5761 ccgatcgttg tcagaagtaa gttggccgca gtgttatcac tcatggttat ggcagcactg
5821 cataattctc ttactgtcat gccatccgta agatgctttt ctgtgactgg tgagtactca
5881 accaagtcat tctgagaata gtgtatgcgg cgaccgagtt gctcttgccc ggcgtcaata
5941 cgggataata ccgcgccaca tagcagaact ttaaaagtgc tcatcattgg aaaacgttct
6001 tcggggcgaa aactctcaag gatcttaccg ctgttgagat ccagttcgat gtaacccact
6061 cgtgcaccca actgatcttc agcatctttt actttcacca gcgtttctgg gtgagcaaaa
6121 acaggaaggc aaaatgccgc aaaaaaggga ataagggcga cacggaaatg ttgaatactc
6181 atactcttcc tttttcaata ttattgaagc atttatcagg gttattgtct catgagcgga
6241 tacatatttg aatgtattta gaaaaataaa caaatagggg ttccgcgcac atttccccga
6301 aaagtgccac ctgacgtcta agaaaccatt attatcatga cattaaccta taaaaatagg
6361 cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg acggtgaaaa cctctgacac
6421 atgcagctcc cggagacggt cacagcttgt ctgtaagcgg atgccgggag cagacaagcc
6481 cgtcagggcg cgtcagcggg tgttggcggg tgtcggggct ggcttaacta tgcggcatca
6541 gagcagattg tactgagagt gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg
6601 agaaaatacc gcatcaggcg ccattcgcca ttcaggctgc gcaactgttg ggaagggcga
6661 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
6721 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
6781 aagctgacgc gtgtagtctt atgcaatact cttgtagtct tgcaacatgg taacgatgag
6841 ttagcaacat gccttacaag gagagaaaaa gcaccgtgca tgccgattgg tggaagtaag
6901 gtggtacgat cgtgccttat taggaaggca acagacgggt ctgacatgga ttggacgaac
6961 cactgaattg ccgcattgca gagatattgt atttaagtgc ctagctcgat acataaacgg
7021 gtctctctgg ttagaccaga tctgagcctg ggagctctct ggctaactag ggaacccact
7081 gcttaagcct caataaagct tgccttgagt gcttcaagta gtgtgtgccc gtctgttgtg
7141 tgactctggt aactagagat ccctcagacc cttttagtca gtgtggaaaa tctctagcag
7201 tggcgcccga acagggactt gaaagcgaaa gggaaaccag aggagctctc tcgacgcagg
7261 actcggcttg ctgaagcgcg cacggcaaga ggcgaggggc ggcgactggt gagtacgcca
7321 aaaattttga ctagcggagg ctagaaggag agagatgggt gcgagagcgt cagtattaag
7381 cgggggagaa ttagatcgcg atgggaaaaa attcggttaa ggccaggggg aaagaaaaaa
7441 tataaattaa aacatatagt atgggcaagc agggagctag aacgattcgc agttaatcct
7501 ggcctgttag aaacatcaga aggctgtaga caaatactgg gacagctaca accatccctt
7561 cagacaggat cagaagaact tagatcatta tataatacag tagcaaccct ctattgtgtg
7621 catcaaagga tagagataaa agacaccaag gaagctttag acaagataga ggaagagcaa
7681 aacaaaagta agaccaccgc acagcaagcg gccactgatc ttcagacctg gaggaggaga
7741 tatgagggac aattggagaa gtgaattata taaatataaa gtagtaaaaa ttgaaccatt
7801 aggagtagca cccaccaagg caaagagaag agtggtgcag agagaaaaaa gagcagtggg
7861 aataggagct ttgttccttg ggttcttggg agcagcagga agcactatgg gcgcagcgtc
7921 aatgacgctg acggtacagg ccagacaatt attgtctggt atagtgcagc agcagaacaa
7981 tttgctgagg gctattgagg cgcaacagca tctgttgcaa ctcacagtct ggggcatcaa
8041 gcagctccag gcaagaatcc tggctgtgga aagataccta aaggatcaac agctcctggg
8101 gatttggggt tgctctggaa aactcatttg caccactgct gtgccttgga atgctagttg
8161 gagtaataaa tctctggaac agat
//
