| 质粒名称: | pT3-EF1A-MYC-IRES-luc |
|---|---|
| 出品公司： | addgene |
| 目录编号: | 129775 |
| 存储实验室: | Amaia Lujambio |
|
载体骨架基本信息 |
|
| 载体名称: | pT3-EF1a |
| 空载体大小: | 9144bp |
| 完整质粒大小: | 9375 bp |
| 载体类型: | 哺乳细胞表达 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | NEB Stable |
| 拷贝数: | 低拷贝 |
|
插入基因信息 |
|
| 插入基因名称: | human MYC/MYC |
| 物种来源: | H. sapiens (human) |
| 插入大小: | 3700 bp |
| 标签/融合蛋白: | luciferase (C terminal on insert) |
| 克隆方法: | Gibson Cloning |
| 5’测序引物 | TCAAGCCTCAGACAGTGGTTC |
|
文献引用方法 |
|
| 材料和方法部分: | pT3-EF1A-MYC-IRES-luc was a gift from Amaia Lujambio (Addgene plasmid # 129775 ; http://n2t.net/addgene:129775 ; RRID:Addgene_129775) |
| 参考文献部分: | beta-catenin activation promotes immune escape and resistance to anti-PD-1 therapy in hepatocellular carcinoma. Ruiz de Galarreta M, Bresnahan E, Molina-Sanchez P, Lindblad KE, Maier B, Sia D, Puigvehi M, Miguela V, Casanova-Acebes M, Dhainaut M, Villacorta-Martin C, Singhi AD, Moghe A, von Felden J, Tal Grinspan L, Wang S, Kamphorst AO, Monga SP, Brown BD, Villanueva A, Llovet JM, Merad M, Lujambio A. Cancer Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi: 10.1158/2159-8290.CD-19-0074. 10.1158/2159-8290.CD-19-0074 PubMed 31186238 |
买家导航
pT3-EF1A-MYC-IRES-luc载体质粒基本信息
pT3-EF1A-MYC-IRES-luc质粒图谱载体图谱和pT3-EF1A-MYC-IRES-luc载体序列质粒序列多克隆位点信息
pT3-EF1A-MYC-IRES-luc质粒载体简介
pT3-EF1A-MYC-IRES-luc质粒序列载体序列
LOCUS pT3-EF1A-MYC-IRES-luc 9145 bp ds-DNA circular SYN 12-MAY-2021
DEFINITION Transposon based vector that expresses human MYC followed by IRES
and firefly luciferase.
ACCESSION .
VERSION .
KEYWORDS pT3-EF1A-MYC-IRES-luc
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 9145)
AUTHORS Ruiz de Galarreta M, Bresnahan E, Molina-Sanchez P, Lindblad KE,
Maier B, Sia D, Puigvehi M, Miguela V, Casanova-Acebes M, Dhainaut
M, Villacorta-Martin C, Singhi AD, Moghe A, von Felden J, Tal
Grinspan L, Wang S, Kamphorst AO, Monga SP, Brown BD, Villanueva A,
Llovet JM, Merad M, Lujambio A
TITLE beta-catenin activation promotes immune escape and resistance to
anti-PD-1 therapy in hepatocellular carcinoma.
JOURNAL Cancer Discov. 2019 Jun 11. pii: 2159-8290.CD-19-0074. doi:
10.1158/2159-8290.CD-19-0074.
PUBMED 31186238
REFERENCE 2 (bases 1 to 9145)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported May 12, 2021 from SnapGene Server 1.1.58
http://www.biofeng.com
FEATURES Location/Qualifiers
source 1..9145
/organism="synthetic DNA construct"
/mol_type="other DNA"
protein_bind complement(34..67)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
promoter 208..1386
/label=EF-1-alpha promoter
/note="strong constitutive promoter for human elongation
factor EF-1-alpha"
intron 439..1377
/label=EF-1-alpha intron A
/note="intron upstream of the start codon of human
EF-1-alpha"
primer_bind 1334..1354
/label=EF1a-F
/note="Human elongation factor-1a promoter, forward primer"
primer_bind 1403..1422
/label=T7
/note="T7 promoter, forward primer"
promoter 1403..1421
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
protein_bind 1500..1524
/gene="mutant version of attB"
/label=attB1
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 1533..2849
/codon_start=1
/product="human c-Myc proto-oncogene"
/label=c-Myc
/translation="MPLNVSFTNRNYDLDYDSVQPYFYCDEEENFYQQQQQSELQPPAP
SEDIWKKFELLPTPPLSPSRRSGLCSPSYVAVTPFSLRGDNDGGGGSFSTADQLEMVTE
LLGGDMVNQSFICDPDDETFIKNIIIQDCMWSGFSAAAKLVSEKLASYQAARKDSGSPN
PARGHSVCSTSSLYLQDLSAAASECIDPSVVFPYPLNDSSSPKSCASQDSSAFSPSSDS
LLSSTESSPQGSPEPLVLHEETPPTTSSDSEEEQEDEEEIDVVSVEKRQAPGKRSESGS
PSAGGHSKPPHSPLVLKRCHVSTHQHNYAAPPSTRKDYPAAKRVKLDSVRVLRQISNNR
KCTSPRSSDTEENVKRRTHNVLERQRRNELKRSFFALRDQIPELENNEKAPKVVILKKA
TAYILSVQAEEQKLISEEDLLRKRREQLKHKLEQLRNSCA"
protein_bind complement(2851..2875)
/gene="mutant version of attB"
/label=attB2
/bound_moiety="BP Clonase(TM)"
/note="recombination site for the Gateway(R) BP reaction"
CDS 2928..2969
/codon_start=1
/product="epitope tag from simian virus 5"
/label=V5 tag
/translation="GKPIPNPLLGLDST"
misc_feature 2991..3555
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
misc_feature 3005..3557
/label=IRES
/note="internal ribosome entry site (IRES) of the
encephalomyocarditis virus (EMCV)"
primer_bind complement(3149..3166)
/label=IRES reverse
/note="IRES internal ribosome entry site, reverse primer.
Also called pCDH-rev"
primer_bind 3376..3395
/label=IRES-F
/note="IRES internal ribosome entry site, forward primer"
CDS 3569..5221
/codon_start=1
/gene="luc+"
/product="firefly luciferase"
/label=luciferase
/note="enhanced luc+ version of the luciferase gene"
/translation="MEDAKNIKKGPAPFYPLEDGTAGEQLHKAMKRYALVPGTIAFTDA
HIEVDITYAEYFEMSVRLAEAMKRYGLNTNHRIVVCSENSLQFFMPVLGALFIGVAVAP
ANDIYNERELLNSMGISQPTVVFVSKKGLQKILNVQKKLPIIQKIIIMDSKTDYQGFQS
MYTFVTSHLPPGFNEYDFVPESFDRDKTIALIMNSSGSTGLPKGVALPHRTACVRFSHA
RDPIFGNQIIPDTAILSVVPFHHGFGMFTTLGYLICGFRVVLMYRFEEELFLRSLQDYK
IQSALLVPTLFSFFAKSTLIDKYDLSNLHEIASGGAPLSKEVGEAVAKRFHLPGIRQGY
GLTETTSAILITPEGDDKPGAVGKVVPFFEAKVVDLDTGKTLGVNQRGELCVRGPMIMS
GYVNNPEATNALIDKDGWLHSGDIAYWDEDEHFFIVDRLKSLIKYKGYQVAPAELESIL
LQHPNIFDAGVAGLPDDDAGELPAAVVVLEHGKTMTEKEIVDYVASQVTTAKKLRGGVV
FVDEVPKGLTGKLDARKIREILIKAKKGGKIAV"
primer_bind complement(3635..3653)
/label=LucNrev
/note="Firefly luciferase, reverse primer"
primer_bind complement(5274..5291)
/label=BGH-rev
/note="Bovine growth hormone terminator, reverse primer.
Also called BGH reverse"
polyA_signal 5280..5504
/label=bGH poly(A) signal
/note="bovine growth hormone polyadenylation signal"
protein_bind complement(5574..5607)
/label=loxP
/bound_moiety="Cre recombinase"
/note="Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
primer_bind 5663..5683
/label=pBABE 3'
/note="SV40 enhancer, reverse primer for pBABE vectors"
primer_bind complement(6065..6082)
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind complement(6065..6081)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(6074..6096)
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind complement(6290..6309)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 6409..6431
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
primer_bind complement(6469..6487)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 6555..6659
/gene="bla"
/label=AmpR promoter
CDS 6660..7520
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(6878..6897)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 7691..8279
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8180..8199
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8433..8450
/label=L4440
/note="L4440 vector, forward primer"
protein_bind 8567..8588
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 8603..8633
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 8641..8657
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 8646..8668
/label=M13/pUC Reverse
/note="In lacZ gene"
primer_bind 8665..8681
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind 8665..8681
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(9107..9126)
/label=EBV-rev
/note="SV40 polyA terminator, reverse primer"
ORIGIN
1 ctagctcgag atcttaatta aactagtatc gatataactt cgtataatgt atgctatacg
61 aagttatatg tacgggccag atatacgcgt tgacattgat tattgactag gcttttgcaa
121 aaagctttgc aaagatggat aaagttttaa acagagagga atctttgcag ctaatggacc
181 ttctaggtct tgaaaggagt gggaattggc tccggtgccc gtcagtgggc agagcgcaca
241 tcgcccacag tccccgagaa gttgggggga ggggtcggca attgaaccgg tgcctagaga
301 aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact ggctccgcct ttttcccgag
361 ggtgggggag aaccgtatat aagtgcagta gtcgccgtga acgttctttt tcgcaacggg
421 tttgccgcca gaacacaggt aagtgccgtg tgtggttccc gcgggcctgg cctctttacg
481 ggttatggcc cttgcgtgcc ttgaattact tccacctggc tgcagtacgt gattcttgat
541 cccgagcttc gggttggaag tgggtgggag agttcgaggc cttgcgctta aggagcccct
601 tcgcctcgtg cttgagttga ggcctggcct gggcgctggg gccgccgcgt gcgaatctgg
661 tggcaccttc gcgcctgtct cgctgctttc gataagtctc tagccattta aaatttttga
721 tgacctgctg cgacgctttt tttctggcaa gatagtcttg taaatgcggg ccaagatctg
781 cacactggta tttcggtttt tggggccgcg ggcggcgacg gggcccgtgc gtcccagcgc
841 acatgttcgg cgaggcgggg cctgcgagcg cggccaccga gaatcggacg ggggtagtct
901 caagctggcc ggcctgctct ggtgcctggc ctcgcgccgc cgtgtatcgc cccgccctgg
961 gcggcaaggc tggcccggtc ggcaccagtt gcgtgagcgg aaagatggcc gcttcccggc
1021 cctgctgcag ggagctcaaa atggaggacg cggcgctcgg gagagcgggc gggtgagtca
1081 cccacacaaa ggaaaagggc ctttccgtcc tcagccgtcg cttcatgtga ctccacggag
1141 taccgggcgc cgtccaggca cctcgattag ttctcgagct tttggagtac gtcgtcttta
1201 ggttgggggg aggggtttta tgcgatggag tttccccaca ctgagtgggt ggagactgaa
1261 gttaggccag cttggcactt gatgtaattc tccttggaat ttgccctttt tgagtttgga
1321 tcttggttca ttctcaagcc tcagacagtg gttcaaagtt tttttcttcc atttcaggtg
1381 tcgtgaggaa ttagcttggt actaatacga ctcactatag ggagacccaa gctggctagg
1441 taagcttggt accgagctcg gatccactag tccagtgtgg tggaattctg cagatatcaa
1501 caagtttgta caaaaaagca ggcttcgcca ccatgcccct caacgttagc ttcaccaaca
1561 ggaactatga cctcgactac gactcggtgc agccgtattt ctactgcgac gaggaggaga
1621 acttctacca gcagcagcag cagagcgagc tgcagccccc ggcgcccagc gaggatatct
1681 ggaagaaatt cgagctgctg cccaccccgc ccctgtcccc tagccgccgc tccgggctct
1741 gctcgccctc ctacgttgcg gtcacaccct tctcccttcg gggagacaac gacggcggtg
1801 gcgggagctt ctccacggcc gaccagctgg agatggtgac cgagctgctg ggaggagaca
1861 tggtgaacca gagtttcatc tgcgacccgg acgacgagac cttcatcaaa aacatcatca
1921 tccaggactg tatgtggagc ggcttctcgg ccgccgccaa gctcgtctca gagaagctgg
1981 cctcctacca ggctgcgcgc aaagacagcg gcagcccgaa ccccgcccgc ggccacagcg
2041 tctgctccac ctccagcttg tacctgcagg atctgagcgc cgccgcctca gagtgcatcg
2101 acccctcggt ggtcttcccc taccctctca acgacagcag ctcgcccaag tcctgcgcct
2161 cgcaagactc cagcgccttc tctccgtcct cggattctct gctctcctcg acggagtcct
2221 ccccgcaggg cagccccgag cccctggtgc tccatgagga gacaccgccc accaccagca
2281 gcgactctga ggaggaacaa gaagatgagg aagaaatcga tgttgtttct gtggaaaaga
2341 ggcaggctcc tggcaaaagg tcagagtctg gatcaccttc tgctggaggc cacagcaaac
2401 ctcctcacag cccactggtc ctcaagaggt gccacgtctc cacacatcag cacaactacg
2461 cagcgcctcc ctccactcgg aaggactatc ctgctgccaa gagggtcaag ttggacagtg
2521 tcagagtcct gagacagatc agcaacaacc gaaaatgcac cagccccagg tcctcggaca
2581 ccgaggagaa tgtcaagagg cgaacacaca acgtcttgga gcgccagagg aggaacgagc
2641 taaaacggag cttttttgcc ctgcgtgacc agatcccgga gttggaaaac aatgaaaagg
2701 cccccaaggt agttatcctt aaaaaagcca cagcatacat cctgtccgtc caagcagagg
2761 agcaaaagct catttctgaa gaggacttgt tgcggaaacg acgagaacag ttgaaacaca
2821 aacttgaaca gctacggaac tcttgtgcgg acccagcttt cttgtacaaa gtggttgata
2881 tccagcacag tggcggccgc tcgagtctag agggcccgcg gttcgaaggt aagcctatcc
2941 ctaaccctct cctcggtctc gattctacgc gtaccggtta gtaatgagtt tccccccccc
3001 cctaacgtta ctggccgaag ccgcttggaa taaggccggt gtgcgtttgt ctatatgtta
3061 ttttccacca tattgccgtc ttttggcaat gtgagggccc ggaaacctgg ccctgtcttc
3121 ttgacgagca ttcctagggg tctttcccct ctcgccaaag gaatgcaagg tctgttgaat
3181 gtcgtgaagg aagcagttcc tctggaagct tcttgaagac aaacaacgtc tgtagcgacc
3241 ctttgcaggc agcggaaccc cccacctggc gacaggtgcc tctgcggcca aaagccacgt
3301 gtataagata cacctgcaaa ggcggcacaa ccccagtgcc acgttgtgag ttggatagtt
3361 gtggaaagag tcaaatggct ctcctcaagc gtattcaaca aggggctgaa ggatgcccag
3421 aaggtacccc attgtatggg atctgatctg gggcctcggt gcacatgctt tacatgtgtt
3481 tagtcgaggt taaaaaaacg tctaggcccc ccgaaccacg gggacgtggt tttcctttga
3541 aaaacacgat gataatatgg ccacaaccat ggaagacgcc aaaaacataa agaaaggccc
3601 ggcgccattc tatccgctgg aagatggaac cgctggagag caactgcata aggctatgaa
3661 gagatacgcc ctggttcctg gaacaattgc ttttacagat gcacatatcg aggtggacat
3721 cacttacgct gagtacttcg aaatgtccgt tcggttggca gaagctatga aacgatatgg
3781 gctgaataca aatcacagaa tcgtcgtatg cagtgaaaac tctcttcaat tctttatgcc
3841 ggtgttgggc gcgttattta tcggagttgc agttgcgccc gcgaacgaca tttataatga
3901 acgtgaattg ctcaacagta tgggcatttc gcagcctacc gtggtgttcg tttccaaaaa
3961 ggggttgcaa aaaattttga acgtgcaaaa aaagctccca atcatccaaa aaattattat
4021 catggattct aaaacggatt accagggatt tcagtcgatg tacacgttcg tcacatctca
4081 tctacctccc ggttttaatg aatacgattt tgtgccagag tccttcgata gggacaagac
4141 aattgcactg atcatgaact cctctggatc tactggtctg cctaaaggtg tcgctctgcc
4201 tcatagaact gcctgcgtga gattctcgca tgccagagat cctatttttg gcaatcaaat
4261 cattccggat actgcgattt taagtgttgt tccattccat cacggttttg gaatgtttac
4321 tacactcgga tatttgatat gtggatttcg agtcgtctta atgtatagat ttgaagaaga
4381 gctgtttctg aggagccttc aggattacaa gattcaaagt gcgctgctgg tgccaaccct
4441 attctccttc ttcgccaaaa gcactctgat tgacaaatac gatttatcta atttacacga
4501 aattgcttct ggtggcgctc ccctctctaa ggaagtcggg gaagcggttg ccaagaggtt
4561 ccatctgcca ggtatcaggc aaggatatgg gctcactgag actacatcag ctattctgat
4621 tacacccgag ggggatgata aaccgggcgc ggtcggtaaa gttgttccat tttttgaagc
4681 gaaggttgtg gatctggata ccgggaaaac gctgggcgtt aatcaaagag gcgaactgtg
4741 tgtgagaggt cctatgatta tgtccggtta tgtaaacaat ccggaagcga ccaacgcctt
4801 gattgacaag gatggatggc tacattctgg agacatagct tactgggacg aagacgaaca
4861 cttcttcatc gttgaccgcc tgaagtctct gattaagtac aaaggctatc aggtggctcc
4921 cgctgaattg gaatccatct tgctccaaca ccccaacatc ttcgacgcag gtgtcgcagg
4981 tcttcccgac gatgacgccg gtgaacttcc cgccgccgtt gttgttttgg agcacggaaa
5041 gacgatgacg gaaaaagaga tcgtggatta cgtcgccagt caagtaacaa ccgcgaaaaa
5101 gttgcgcgga ggagttgtgt ttgtggacga agtaccgaaa ggtcttaccg gaaaactcga
5161 cgcaagaaaa atcagagaga tcctcataaa ggccaagaag ggcggaaaga tcgccgtgta
5221 attctaggat ccgtcgacct gcagccaagc ttatcgataa acccgctgat cagcctcgac
5281 tgtgccttct agttgccagc catctgttgt ttgcccctcc cccgtgcctt ccttgaccct
5341 ggaaggtgcc actcccactg tcctttccta ataaaatgag gaaattgcat cgcattgtct
5401 gagtaggtgt cattctattc tggggggtgg ggtggggcag gacagcaagg gggaggattg
5461 ggaagacaat agcaggcatg ctggggatgc ggtgggctct atggcttctg aggcggaaag
5521 aaccagctgg ggctctaggg ggtatcccca cgcgccctgt agcggcgcat taaataactt
5581 cgtataatgt atgctatacg aagttatatc gtatgcatgc tttgcatact tctgcctgct
5641 ggggagcctg gggactttcc acaccctaac tgacacacat tccacagaat tccttgaaat
5701 acatccacag gtacacctcc aattgactca aatgatgtca attagtctat cagaagcttc
5761 taaagccatg acatcatttt ctggaatttt ccaagctgtt taaaggcaca gtcaacttag
5821 tgtatgtaaa cttctgaccc actggaattg tgatacagtg aattataagt gaaataatct
5881 gtctgtaaac aattgttgga aaaatgactt gtgtcatgca caaagtagat gtcctaactg
5941 acttgccaaa actattgttt gttaacaaga aatttgtgga gtagttgaaa aacgagtttt
6001 aatgactcca acttaagtgt atgtaaactt ccgacttcaa ctgtagggta ccgagctcga
6061 attcactggc cgtcgtttta caacgtcgtg actgggaaaa ccctggcgtt acccaactta
6121 atcgccttgc agcacatccc cctttcgcca gctggcgtaa tagcgaagag gcccgcaccg
6181 atcgcccttc ccaacagttg cgcagcctga atggcgaatg gcgcctgatg cggtattttc
6241 tccttacgca tctgtgcggt atttcacacc gcatatggtg cactctcagt acaatctgct
6301 ctgatgccgc atagttaagc cagccccgac acccgccaac acccgctgac gcgccctgac
6361 gggcttgtct gctcccggca tccgcttaca gacaagctgt gaccgtctcc gggagctgca
6421 tgtgtcagag gttttcaccg tcatcaccga aacgcgcgag acgaaagggc ctcgtgatac
6481 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
6541 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
6601 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
6661 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
6721 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
6781 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
6841 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
6901 gtattgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
6961 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
7021 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
7081 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
7141 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
7201 ctgtagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
7261 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
7321 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
7381 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
7441 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
7501 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
7561 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
7621 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
7681 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
7741 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
7801 taactggctt cagcagagcg cagataccaa atactgttct tctagtgtag ccgtagttag
7861 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
7921 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
7981 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
8041 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
8101 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
8161 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
8221 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
8281 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
8341 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
8401 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
8461 agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
8521 acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
8581 tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
8641 ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
8701 catgcctgca ggtcgactct agacctatac agttgaagtc ggaagtttac atacacttaa
8761 gttggagtca ttaaaactcg tttttcaact actccacaaa tttcttgtta acaaacaata
8821 gttttggcaa gtcagttagg acatctactt tgtgcatgac acaagtcatt tttccaacaa
8881 ttgtttacag acagattatt tcacttataa ttcactgtat cacaattcca gtgggtcaga
8941 agtttacata cactaagttg actgtgcctt taaacagctt ggaaaattcc agaaaatgat
9001 gtcatggctt tagaagcttc tgatagacta attgacatca tttgagtcaa ttggaggtgt
9061 acctgtggat gtatttcaag gggatccaga catgataaga tacattgatg agtttggaca
9121 aaccacaact agaatgcagc ggccg
//
