pCDH-CMV-mCherry-USP10

LOCUS       pCDH-CMV-mCherry-USP10             9570 bp DNA     circular SYN 08-APR-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 9570)
  AUTHORS   15129015045
  TITLE     Direct Submission
  JOURNAL   Exported Aug 3, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..9570
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     primer_bind     1912..1961
                     /label=Primer 4
     enhancer        1946..2249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2250..2453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     CDS             2504..3211
                     /codon_start=1
                     /product="monomeric derivative of DsRed fluorescent protein
                     (Shaner et al., 2004)"
                     /label=mCherry
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEEDNMAIIKEFMRFKVHMEGSVNGHEFEIEGEGEGRPYEG
                     TQTAKLKVTKGGPLPFAWDILSPQFMYGSKAYVKHPADIPDYLKLSFPEGFKWERVMNL
                     EDGGVVTVTQDSSLQDGEFIYKVKLRGTNFPSDGPVMQKKTMGWEASSERMYPEDGALK
                     GEIKQRLKLKDGGHYDAEVKTTYKAKKPVQLPGAYNVNIKLDITSHNEDYTIVEQYERA
                     EGRHSTGGMDELYK"
     misc_feature    3212..3240
                     /label=MCS
                     /note="multiple cloning site"
     misc_feature    3227..3265
                     /label=Primer F for USP10 in PCDH-mCherry
     CDS             3248..5644
                     /codon_start=1
                     /label=USP10
                     /translation="MALHSPQYIFGDFSPDEFNQFFVTPRSSVELPPYSGTVLCGTQAV
                     DKLPDGQEYQRIEFGVDEVIEPSDTLPRTPSYSISSTLNPQAPEFILGCTASKITPDGI
                     TKEASYGSIDCQYPGSALALDGSSNVEAEVLENDGVSGGLGQRERKKKKKRPPGYYSYL
                     KDGGDDSISTEALVNGHANSAVPNSVSAEDAEFMGDMPPSVTPRTCNSPQNSTDSVSDI
                     VPDSPFPGALGSDTRTAGQPEGGPGADFGQSCFPAEAGRDTLSRTAGAQPCVGTDTTEN
                     LGVANGQILESSGEGTATNGVELHTTESIDLDPTKPESASPPADGTGSASGTLPVSQPK
                     SWASLFHDSKPSSSSPVAYVETKYSPPAISPLVSEKQVEVKEGLVPVSEDPVAIKIAEL
                     LENVTLIHKPVSLQPRGLINKGNWCYINATLQALVACPPMYHLMKFIPLYSKVQRPCTS
                     TPMIDSFVRLMNEFTNMPVPPKPRQALGDKIVRDIRPGAAFEPTYIYRLLTVNKSSLSE
                     KGRQEDAEEYLGFILNGLHEEMLNLKKLLSPSNEKLTISNGPKNHSVNEEEQEEQGEGS
                     EDEWEQVGPRNKTSVTRQADFVQTPITGIFGGHIRSVVYQQSSKESATLQPFFTLQLDI
                     QSDKIRTVQDALESLVARESVQGYTTKTKQEVEISRRVTLEKLPPVLVLHLKRFVYEKT
                     GGCQKLIKNIEYPVDLEISKELLSPGVKNKNFKCHRTYRLFAVVYHHGNSATGGHYTTD
                     VFQIGLNGWLRIDDQTVKVINQYQVVKPTAERTAYLLYYRRVDLL"
     misc_feature    complement(5627..5665)
                     /label=Primer R for USP10 in PCDH-mCherry
     misc_feature    5651..6238
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(6122..6133)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             6311..6544
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    6616..6737
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      6756..6912
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     rep_origin      6778..6798
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(6946..6961)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    6969..6985
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(6993..7023)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    7038..7059
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(7347..7935)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(8106..8966)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(8967..9071)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     9545..9561
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     2461 tgaaccgtca gattctagac cgctagcgct accggtcgcc accatggtga gcaagggcga
     2521 ggaggataac atggccatca tcaaggagtt catgcgcttc aaggtgcaca tggagggctc
     2581 cgtgaacggc cacgagttcg agatcgaggg cgagggcgag ggccgcccct acgagggcac
     2641 ccagaccgcc aagctgaagg tgaccaaggg tggccccctg cccttcgcct gggacatcct
     2701 gtcccctcag ttcatgtacg gctccaaggc ctacgtgaag caccccgccg acatccccga
     2761 ctacttgaag ctgtccttcc ccgagggctt caagtgggag cgcgtgatga acttggagga
     2821 cggcggcgtg gtgaccgtga cccaggactc ctccctgcag gacggcgagt tcatctacaa
     2881 ggtgaagctg cgcggcacca acttcccctc cgacggcccc gtaatgcaga agaagaccat
     2941 gggctgggag gcctcctccg agcggatgta ccccgaggac ggcgccctga agggcgagat
     3001 caagcagagg ctgaagctga aggacggcgg ccactacgac gctgaggtca agaccaccta
     3061 caaggccaag aagcccgtgc agctgcccgg cgcctacaac gtcaacatca agttggacat
     3121 cacctcccac aacgaggact acaccatcgt ggaacagtac gaacgcgccg agggccgcca
     3181 ctccaccggc ggcatggacg agctgtacaa gtccggactc agatctcgag ctcaagcttc
     3241 ggaattcatg gccctccaca gcccgcagta tatttttgga gattttagcc ctgatgaatt
     3301 caatcaattc tttgtgactc ctcgatcttc agttgagctt cctccataca gtggaacagt
     3361 tctgtgtggc acacaggctg tggataaact acctgatgga caagaatatc agagaattga
     3421 gtttggtgtc gatgaagtca ttgaacccag tgacactttg ccgagaaccc ccagctacag
     3481 tatttcaagc acactgaacc ctcaggcccc tgaatttatt ctcggttgta cagcttccaa
     3541 aataacccct gatggtatca ctaaagaagc aagctatggc tccatcgact gccagtaccc
     3601 aggctctgcc ctcgctttgg atggaagttc taatgtggag gcggaagttt tggaaaatga
     3661 tggtgtctca ggtggtcttg gacaaaggga gcgtaaaaag aagaaaaagc ggccacctgg
     3721 atattacagc tatttgaaag atggtggcga tgatagtatc tccacagaag ccctggtcaa
     3781 tggccatgcc aattcagcag tcccgaacag tgtcagtgca gaggatgcag aatttatggg
     3841 tgacatgccc ccgtcagtta cgcccaggac ttgtaacagc ccccagaact ccacagactc
     3901 tgtcagtgac attgtgcctg acagtccttt ccccggagca ctcggcagtg acaccaggac
     3961 tgcagggcag ccagaggggg gccccggggc tgattttggt cagtcctgct tccctgcaga
     4021 ggctggcaga gacaccctgt caaggacagc tggggctcag ccctgcgttg gtaccgatac
     4081 tactgaaaac cttggagttg ctaatggaca aatacttgaa tcctcgggtg agggcacagc
     4141 taccaacggg gtggagttgc acaccacgga aagcatagac ttggacccaa ccaaacccga
     4201 gagtgcatca cctcctgctg acggcacggg ctctgcatca ggcacccttc ctgtcagcca
     4261 gcccaagtcc tgggccagcc tctttcatga ttctaagccc tcttcctcct cgccggtggc
     4321 ctatgtggaa actaagtatt cccctcccgc catatctccc ctggtttctg aaaagcaggt
     4381 tgaagtcaaa gaagggcttg ttccggtttc agaggatcct gtagccataa agattgcaga
     4441 gttgctggag aatgtaaccc taatccataa accagtgtcg ttgcaacccc gtgggctgat
     4501 caataaaggg aactggtgct acattaatgc tacactgcag gcattggttg cttgcccgcc
     4561 gatgtaccac ctgatgaagt tcattcctct gtattccaaa gtgcaaaggc cttgtacgtc
     4621 aacacccatg atagacagct ttgttcggct aatgaatgag ttcactaata tgccagtacc
     4681 tccaaaaccc cgacaagctc ttggagataa aatcgtgagg gatattcgcc ctggagctgc
     4741 ctttgagccc acatatattt acagactcct gacagttaac aagtcaagcc tgtctgaaaa
     4801 gggtcgacag gaagatgctg aggaatactt aggcttcatt ctaaatggac ttcatgagga
     4861 aatgttgaac ctaaagaagc ttctctcacc aagtaatgaa aaacttacga tttccaacgg
     4921 ccccaaaaac cactcggtca atgaagaaga gcaggaagaa caaggtgaag gaagcgagga
     4981 tgaatgggaa caagtgggcc cccggaacaa gacttccgtc acccgccagg cggattttgt
     5041 tcagactcca atcaccggca tttttggtgg acacatcagg tctgtggttt accagcagag
     5101 ttcaaaagaa tctgccactt tgcagccatt tttcacgttg cagttggata tccagtcaga
     5161 caagatacgc acagtccagg atgcactgga gagcttggtg gcaagagaat ctgtccaagg
     5221 ttataccaca aaaaccaaac aagaggttga gataagtcga agagtgactc tggaaaaact
     5281 ccctcctgtc ctcgtgctgc acctgaaacg attcgtttat gagaagactg gtgggtgcca
     5341 gaagcttatc aaaaatattg aatatcctgt ggacttggaa attagtaaag aactgctttc
     5401 tccaggggtt aaaaataaga attttaaatg ccaccgaacc tatcggctgt ttgcagtggt
     5461 ctaccatcac ggcaacagtg cgacgggcgg ccattacact acagacgtct tccagatcgg
     5521 tctgaatggc tggctgcgca tcgatgacca gacagtcaag gtgatcaacc agtaccaggt
     5581 ggtgaaacca actgctgaac gcacagccta cctcctgtat taccgccgag tggacctgct
     5641 gtaaggatcc aatcaacctc tggattacaa aatttgtgaa agattgactg gtattcttaa
     5701 ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt atcatgctat
     5761 tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttgc tgtctcttta
     5821 tgaggagttg tggcccgttg tcaggcaacg tggcgtggtg tgcactgtgt ttgctgacgc
     5881 aacccccact ggttggggca ttgccaccac ctgtcagctc ctttccggga ctttcgcttt
     5941 ccccctccct attgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
     6001 ggctcggctg ttgggcactg acaattccgt ggtgttgtcg gggaaatcat cgtcctttcc
     6061 ttggctgctc gcctgtgttg ccacctggat tctgcgcggg acgtccttct gctacgtccc
     6121 ttcggccctc aatccagcgg accttccttc ccgcggcctg ctgccggctc tgcggcctct
     6181 tccgcgtctc cgccttcgcc ctcagacgag tcggatctcc ctttggccgc ctccccgccg
     6241 gtacctttaa gaccaatgac ttacaaggca gctgtagatc ttagccactt tttaaaagaa
     6301 aaggggggac tggaagggct aattcactcc caacgaagat aagatctgct ttttgcttgt
     6361 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac
     6421 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg
     6481 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct
     6541 agcagtagta gttcatgtca tcttattatt cagtatttat aacttgcaaa gaaatgaata
     6601 tcagagagtg agaggaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     6661 atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     6721 ctcatcaatg tatcttatca tgtctggctc tagctatccc gcccctaact ccgcccatcc
     6781 cgcccctaac tccgcccagt tccgcccatt ctccgcccca tggctgacta atttttttta
     6841 tttatgcaga ggccgaggcg gcctcggcct ctgagctatt ccagaagtag tgaggaggct
     6901 tttttggagg cctagacttt tgcagagacc aaattcgtaa tcatgtcata gctgtttcct
     6961 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
     7021 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
     7081 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
     7141 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
     7201 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
     7261 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
     7321 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
     7381 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
     7441 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
     7501 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
     7561 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
     7621 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
     7681 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
     7741 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
     7801 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
     7861 aaacaaacca ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga
     7921 aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac
     7981 gaaaactcac gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc
     8041 cttttaaatt aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct
     8101 gacagttacc aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca
     8161 tccatagttg cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct
     8221 ggccccagtg ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca
     8281 ataaaccagc cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc
     8341 atccagtcta ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg
     8401 cgcaacgttg ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct
     8461 tcattcagct ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa
     8521 aaagcggtta gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta
     8581 tcactcatgg ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc
     8641 ttttctgtga ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg
     8701 agttgctctt gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa
     8761 gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg
     8821 agatccagtt cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc
     8881 accagcgttt ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg
     8941 gcgacacgga aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat
     9001 cagggttatt gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata
     9061 ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc
     9121 atgacattaa cctataaaaa taggcgtatc acgaggccct ttcgtctcgc gcgtttcggt
     9181 gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     9241 gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     9301 ggctggctta actatgcggc atcagagcag attgtactga gagtgcacca tatgcggtgt
     9361 gaaataccgc acagatgcgt aaggagaaaa taccgcatca ggcgccattc gccattcagg
     9421 ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg ccagctggcg
     9481 aaagggggat gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga
     9541 cgttgtaaaa cgacggccag tgccaagctg
//