pAAVLINKv1-pCALM1-Cre-DSP-C-FLAG

LOCUS       pAAVLINKv1-pCALM1-Cre-DSP-C-FLAG           6987 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6987)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 8, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6987
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    12..130
                     /label=AAV2 ITR
     promoter        145..265
                     /label=pCALM1
     CDS             277..1308
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     intron          1309..1349
                     /label=Splicing Donor
     protein_bind    1356..1389
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1390..1481
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1482..3700
                     /codon_start=1
                     /label=DSP
                     /translation="GV**TL*TVSFCQKMSPWPGG*LIEICIDP*MIPEIVRKTLWIQS
                     PKRRSVTCS*RNGAESNHILVCSCFQYRREACPSKESDNL*PSLS**ILVY*DRPLSMN
                     WNLVRFLMTRLVRELRTSSRVQAA*QAYTMRPQNRSLAFMRP*KLA*SDLVLLWSCWKP
                     KQLLAL*WILLAT*GYQWRKPTREVWWALSSKRSSCLQNELSLGIMILKQETSSLCSKP
                     *IRNSSKRATVFAY*KHRSQLGGSLTQRRAIVYQLT*HIRGAISMRNSVRFSQIQVMIP
                     KDFLTPTLKKILPICN*KKDALRMRKQGSVFCL*KKRRNRCRHHKRIPSGSVEWS*LTQ
                     KPIKKCLFRRPTRRA*LIMKPSKNCVSRNVNGKK*PSRDQMAPPGWSW*IERQAVSMIF
                     KMLLTRALLTGSSLISTDPAASASLNLLT*SP*KMVSAPAAAWAVVSAMMFLAAPDMNQ
                     *VRFPPYPASGI*P*GAALFQTPWKNRAPLQPSLTQKTWRKSPLQKV*SGASLTASRVR
                     GFWRLRPAQVASSTQPRARSCHFRTQSPRV*LTKTWPPG*SLLRKPS*ASRV*RERRRC
                     QQQRQ*KKNGSRMRLASASWSSSTSREVLLTRKCMGG*APKKPSGRGS*MAAPHRGCKT
                     PAAMPKS*PAPKPN*KYPIRMP*IAPW*KISLGCAFWKPPPCRPRAYPALTTCLRLRAP
                     APAPARDLAPDLAPGPAVGPGEEALTPQGILPTLIPTHLAVVLLG"
     misc_feature    2526..2531
                     /label=SDS
     CDS             3707..3772
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3776..4207
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    3920..3925
                     /label=SDS
     repeat_region   4250..4390
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4250..4379
                     /label=AAV2 ITR
     rep_origin      4465..4920
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5202..5306
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5307..6167
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6338..6926
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
      181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
      241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
      301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
      361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
      421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
      481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
      541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
      601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
      661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
      721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
      781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
      841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
      901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
      961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
     1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
     1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
     1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
     1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
     1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
     1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
     1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
     1441 tattggtctt actgacatcc actttgcctt tctctccaca gggggtgtag tagaccctgt
     1501 gaacagtgtc tttttgccaa aagatgtcgc cttggcccgg gggctgattg atagagattt
     1561 gtatcgatcc ctgaatgatc cccgagatag tcagaaaaac tttgtggatc cagtcaccaa
     1621 aaagaaggtc agttacgtgc agctgaagga acggtgcaga atcgaaccac atactggtct
     1681 gctcttgctt tcagtacaga agagaagcat gtccttccaa ggaatcagac aacctgtgac
     1741 cgtcactgag ctagtagatt ctggtatatt gagaccgtcc actgtcaatg aactggaatc
     1801 tggtcagatt tcttatgacg aggttggtga gagaattaag gacttcctcc agggttcaag
     1861 ctgcatagca ggcatataca atgagaccac aaaacagaag cttggcattt atgaggccat
     1921 gaaaattggc ttagtccgac ctggtactgc tctggagttg ctggaagccc aagcagctac
     1981 tggctttata gtggatcctg ttagcaactt gaggttacca gtggaggaag cctacaagag
     2041 aggtctggtg ggcattgagt tcaaagagaa gctcctgtct gcagaacgag ctgtcactgg
     2101 gtataatgat cctgaaacag gaaacatcat ctctttgttc caagccatga ataaggaact
     2161 catcgaaaag ggccacggta ttcgcttatt agaagcacag atcgcaactg gggggatcat
     2221 tgacccaaag gagagccatc gtttaccagt tgacatagca tataagaggg gctatttcaa
     2281 tgaggaactc agtgagattc tctcagatcc aagtgatgat accaaaggat tttttgaccc
     2341 caacactgaa gaaaatctta cctatctgca actaaaagaa agatgcatta aggatgagga
     2401 aacagggctc tgtcttctgc ctctgaaaga aaagaagaaa caggtgcaga catcacaaaa
     2461 gaataccctc aggaagcgta gagtggtcat agttgaccca gaaaccaata aagaaatgtc
     2521 tgttcaggag gcctacaaga agggcctaat tgattatgaa accttcaaag aactgtgtga
     2581 gcaggaatgt gaatgggaag aaataaccat cacgggatca gatggctcca ccagggtggt
     2641 cctggtagat agaaagacag gcagtcagta tgatattcaa gatgctattg acaagggcct
     2701 tgttgacagg aagttctttg atcagtaccg atccggcagc ctcagcctca ctcaatttgc
     2761 tgacatgatc tccttgaaaa atggtgtcgg caccagcagc agcatgggca gtggtgtcag
     2821 cgatgatgtt tttagcagct cccgacatga atcagtaagt aagatttcca ccatatccag
     2881 cgtcaggaat ttaaccataa ggagcagctc tttttcagac accctggaag aatcgagccc
     2941 cattgcagcc atctttgaca cagaaaacct ggagaaaatc tccattacag aaggtataga
     3001 gcggggcatc gttgacagca tcacgggtca gaggcttctg gaggctcagg cctgcacagg
     3061 tggcatcatc cacccaacca cgggccagaa gctgtcactt caggacgcag tctcccaggg
     3121 tgtgattgac caagacatgg ccaccaggct gaagcctgct cagaaagcct tcataggctt
     3181 cgagggtgtg aagggaaaga agaagatgtc agcagcagag gcagtgaaag aaaaatggct
     3241 cccgtatgag gctggccagc gcttcctgga gttccagtac ctcacgggag gtcttgttga
     3301 cccggaagtg catgggagga taagcaccga agaagccatc cggaaggggt tcatagatgg
     3361 ccgcgccgca cagaggctgc aagacaccag cagctatgcc aaaatcctga cctgccccaa
     3421 aaccaaatta aaaatatcct ataaggatgc cataaatcgc tccatggtag aagatatcac
     3481 tgggctgcgc cttctggaag ccgcctccgt gtcgtccaag ggcttaccca gcccttacaa
     3541 catgtcttcg gctccgggct cccgctccgg ctcccgctcg ggatctcgct ccggatctcg
     3601 ctccgggtcc cgcagtgggt cccggagagg aagctttgac gccacaggga attcttccta
     3661 ctcttattcc tactcattta gcagtagttc tattgggcac tgtacagact acaaagacca
     3721 tgacggtgat tataaagatc atgacatcga ctacaaggat gacgatgaca agtaactcga
     3781 gataatcaac ctctggatta caaaatttgt gaaagattga ctggtattct taactatgtt
     3841 gctcctttta cgctatgtgg atacgctgct ttaatgcctt tgtatcatgc tattgcttcc
     3901 cgtatggctt tcattttctc ctccttgtat aaatcctggt tagttcttgc cacggcggaa
     3961 ctcatcgccg cctgccttgc ccgctgctgg acaggggctc ggctgttggg cactgacaat
     4021 tccgtggtgt ttatttgtga aatttgtgat gctattgctt tatttgtaac catctagctt
     4081 tatttgtgaa atttgtgatg ctattgcttt atttgtaacc attataagct gcaataaaca
     4141 agttaacaac aacaattgca ttcattttat gtttcaggtt cagggggaga tgtgggaggt
     4201 tttttaaaga tctggagacc ggtgtggcac gtgcggaccg agcggccgca ggaaccccta
     4261 gtgatggagt tggccactcc ctctctgcgc gctcgctcgc tcactgaggc cgggcgacca
     4321 aaggtcgccc gacgcccggg ctttgcccgg gcggcctcag tgagcgagcg agcgcgcagc
     4381 tgcctgcagg ggcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac
     4441 cgcatacgtc aaagcaacca tagtacgcgc cctgtagcgg cgcattaagc gcggcgggtg
     4501 tggtggttac gcgcagcgtg accgctacac ttgccagcgc cttagcgccc gctcctttcg
     4561 ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct ctaaatcggg
     4621 ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa aaacttgatt
     4681 tgggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc cctttgacgt
     4741 tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca ctcaactcta
     4801 tctcgggcta ttcttttgat ttataaggga ttttgccgat ttcggtctat tggttaaaaa
     4861 atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg tttacaattt
     4921 tatggtgcac tctcagtaca atctgctctg atgccgcata gttaagccag ccccgacacc
     4981 cgccaacacc cgctgacgcg ccctgacggg cttgtctgct cccggcatcc gcttacagac
     5041 aagctgtgac cgtctccggg agctgcatgt gtcagaggtt ttcaccgtca tcaccgaaac
     5101 gcgcgagacg aaagggcctc gtgatacgcc tatttttata ggttaatgtc atgataataa
     5161 tggtttctta gacgtcaggt ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt
     5221 tatttttcta aatacattca aatatgtatc cgctcatgag acaataaccc tgataaatgc
     5281 ttcaataata ttgaaaaagg aagagtatga gtattcaaca tttccgtgtc gcccttattc
     5341 ccttttttgc ggcattttgc cttcctgttt ttgctcaccc agaaacgctg gtgaaagtaa
     5401 aagatgctga agatcagttg ggtgcacgag tgggttacat cgaactggat ctcaacagcg
     5461 gtaagatcct tgagagtttt cgccccgaag aacgttttcc aatgatgagc acttttaaag
     5521 ttctgctatg tggcgcggta ttatcccgta ttgacgccgg gcaagagcaa ctcggtcgcc
     5581 gcatacacta ttctcagaat gacttggttg agtactcacc agtcacagaa aagcatctta
     5641 cggatggcat gacagtaaga gaattatgca gtgctgccat aaccatgagt gataacactg
     5701 cggccaactt acttctgaca acgatcggag gaccgaagga gctaaccgct tttttgcaca
     5761 acatggggga tcatgtaact cgccttgatc gttgggaacc ggagctgaat gaagccatac
     5821 caaacgacga gcgtgacacc acgatgcctg tagcaatggc aacaacgttg cgcaaactat
     5881 taactggcga actacttact ctagcttccc ggcaacaatt aatagactgg atggaggcgg
     5941 ataaagttgc aggaccactt ctgcgctcgg cccttccggc tggctggttt attgctgata
     6001 aatctggagc cggtgagcgt gggtctcgcg gtatcattgc agcactgggg ccagatggta
     6061 agccctcccg tatcgtagtt atctacacga cggggagtca ggcaactatg gatgaacgaa
     6121 atagacagat cgctgagata ggtgcctcac tgattaagca ttggtaactg tcagaccaag
     6181 tttactcata tatactttag attgatttaa aacttcattt ttaatttaaa aggatctagg
     6241 tgaagatcct ttttgataat ctcatgacca aaatccctta acgtgagttt tcgttccact
     6301 gagcgtcaga ccccgtagaa aagatcaaag gatcttcttg agatcctttt tttctgcgcg
     6361 taatctgctg cttgcaaaca aaaaaaccac cgctaccagc ggtggtttgt ttgccggatc
     6421 aagagctacc aactcttttt ccgaaggtaa ctggcttcag cagagcgcag ataccaaata
     6481 ctgttcttct agtgtagccg tagttaggcc accacttcaa gaactctgta gcaccgccta
     6541 catacctcgc tctgctaatc ctgttaccag tggctgctgc cagtggcgat aagtcgtgtc
     6601 ttaccgggtt ggactcaaga cgatagttac cggataaggc gcagcggtcg ggctgaacgg
     6661 ggggttcgtg cacacagccc agcttggagc gaacgaccta caccgaactg agatacctac
     6721 agcgtgagct atgagaaagc gccacgcttc ccgaagggag aaaggcggac aggtatccgg
     6781 taagcggcag ggtcggaaca ggagagcgca cgagggagct tccaggggga aacgcctggt
     6841 atctttatag tcctgtcggg tttcgccacc tctgacttga gcgtcgattt ttgtgatgct
     6901 cgtcaggggg gcggagccta tggaaaaacg ccagcaacgc ggccttttta cggttcctgg
     6961 ccttttgctg gccttttgct cacatgt
//