| 质粒名称: | pGWB406 |
|---|---|
| 出品公司: | 爱迪基因 |
| 目录编号: | 74800 |
| 存储实验室: | Tsuyoshi Nakagawa |
|
载体骨架基本信息 |
|
| 载体名称: | pPZP221 |
| 载体出品公司: | Hajdukiewicz P., Svab Z. and Maliga P. |
| 载体抗性: | 氯霉素和大观霉素 |
| 空载体大小: | 8500 bp |
| 完整质粒大小: | -- |
| 载体修饰: | 去除庆大霉素抗性标记和卡那霉素抗性标记（NOS发起人：NPTII：NOS terminator）已插入。 |
| 载体类型: | 植物表达 |
| 筛选标记: | 卡那霉素 |
| 高拷贝/低拷贝: | 高拷贝 |
| 生长条件: | 37 ℃ |
|
插入基因信息 |
|
| 插入基因名称: | -- |
| 物种来源: | -- |
| 插入大小: | -- |
| 基因突变: | -- |
| GenBank编号 | -- |
| 启动子 | CaMV35S |
| 标签/融合蛋白: | -- |
| 克隆方法: | Gateway 克隆 |
| 5' 克隆位点: | -- |
| 3' 克隆位点: | -- |
| 5' 测序引物 | -- |
| 3' 测序引物 | -- |
|
文献引用方法 |
|
| 材料和方法部分: | pGWB406 was a gift from Tsuyoshi Nakagawa (Addgene plasmid # 74800 ; http://n2t.net/addgene:74800 ; RRID:Addgene_74800) |
| 参考文献部分: | Improved Gateway binary vectors: high-performance vectors for creation of fusion constructs in transgenic analysis of plants. Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T, Ishiguro S. Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7. 10.1271/bbb.70216 PubMed 17690442 |
| 相关质粒: |
pGWB4 pGWB5 pGWB11 pGWB12 pGWB17 pGWB18 pGWB21 pGWB406 pGWB453 pGWB454 pGWB455 pGWB504 |
买家导航
pGWB406载体质粒基本信息
pGWB406质粒图谱载体图谱和pGWB406载体序列质粒序列多克隆位点信息
pGWB406质粒载体简介
pGWB406质粒序列载体序列
LOCUS pGWB406 12407 bp ds-DNA circular SYN 06-AUG-2018
DEFINITION Gateway cloning compatible binary vector for N-terminal fusion with
sGFP (CaMV35S promoter)..
ACCESSION .
VERSION .
KEYWORDS pGWB406
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 12407)
AUTHORS Nakagawa T, Suzuki T, Murata S, Nakamura S, Hino T, Maeo K, Tabata
R, Kawai T, Tanaka K, Niwa Y, Watanabe Y, Nakamura K, Kimura T,
Ishiguro S
TITLE Improved Gateway binary vectors: high-performance vectors for
creation of fusion constructs in transgenic analysis of plants.
JOURNAL Biosci Biotechnol Biochem. 2007 Aug;71(8):2095-100. Epub 2007 Aug 7.
PUBMED 17690442
REFERENCE 2 (bases 1 to 12407)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Aug 6, 2018 from SnapGene Server 1.1.58
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..12407
/organism="synthetic DNA construct"
/mol_type="other DNA"
misc_feature 54..78
/label=RB T-DNA repeat
/note="right border repeat from nopaline C58 T-DNA"
primer_bind 266..288
/label=M13/pUC Forward
/note="In lacZ gene"
primer_bind 280..297
/label=M13 Forward
/note="In lacZ gene. Also called M13-F20 or M13 (-21)
Forward"
primer_bind 281..297
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
promoter 812..1157
/label=CaMV 35S promoter
/note="strong constitutive promoter from cauliflower mosaic
virus"
primer_bind 1100..1120
/label=35S promoter
/note="CaMV 35S promoter, forward primer"
CDS 1178..1891
/codon_start=1
/product="enhanced GFP"
/label=EGFP
/note="mammalian codon-optimized"
/translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
KFICTTGKLPVPWPTLVTTFTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
VNFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
EFVTAAGITHGMDELY"
primer_bind complement(1223..1244)
/label=EGFP-N
/note="EGFP, reverse primer"
primer_bind complement(1484..1503)
/label=EXFP-R
/note="For distinguishing EGFP variants, reverse primer"
primer_bind 1831..1852
/label=EGFP-C
/note="EGFP, forward primer"
protein_bind 1895..2019
/gene="mutant version of attR"
/label=attR1
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
promoter 2056..2086
/label=lac UV5 promoter
/note="E. coli lac promoter with an ""up"" mutation"
CDS 2140..2799
/codon_start=1
/gene="cat"
/product="chloramphenicol acetyltransferase"
/label=CmR
/note="confers resistance to chloramphenicol"
/translation="MEKKITGYTTVDISQWHRKEHFEAFQSVAQCTYNQTVQLDITAFL
KTVKKNKHKFYPAFIHILARLMNAHPEFRMAMKDGELVIWDSVHPCYTVFHEQTETFSS
LWSEYHDDFRQFLHIYSQDVACYGENLAYFPKGFIENMFFVSANPWVSFTSFDLNVANM
DNFFAPVFTMGKYYTQGDKVLMPLAIQVHHAVCDGFHVGRMLNELQQYCDEWQGGA"
primer_bind complement(2206..2225)
/label=CAT-R
/note="Chloramphenicol resistance gene, reverse primer"
CDS 3141..3446
/codon_start=1
/gene="ccdB"
/product="CcdB, a bacterial toxin that poisons DNA gyrase"
/label=ccdB
/note="Plasmids containing the ccdB gene cannot be
propagated in standard E. coli strains."
/translation="MQFKVYTYKRESRYRLFVDVQSDIIDTPGRRMVIPLASARLLSDK
VSRELYPVVHIGDESWRMMTTDMASVPVSVIGEEVADLSHRENDIKNAINLMFWGI"
primer_bind 3325..3344
/label=ccdB-fwd
/note="ccdB gene, forward primer"
protein_bind complement(3487..3611)
/gene="mutant version of attR"
/label=attR2
/bound_moiety="LR Clonase(TM)"
/note="recombination site for the Gateway(R) LR reaction"
terminator 3649..3901
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
primer_bind complement(3935..3951)
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
primer_bind complement(3935..3951)
/label=M13 Reverse
/note="In lacZ gene. Also called M13-rev"
primer_bind complement(3948..3970)
/label=M13/pUC Reverse
/note="In lacZ gene"
protein_bind 3959..3975
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
promoter complement(3983..4013)
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 4028..4049
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
terminator 4083..4335
/label=NOS terminator
/note="nopaline synthase terminator and poly(A) signal"
CDS complement(4725..5519)
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/label=NeoR/KanR
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGDDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
primer_bind complement(4836..4855)
/label=Neo-F
/note="Neomycin resistance gene, forward primer"
primer_bind 5446..5465
/label=Neo-R
/note="Neomycin resistance gene, reverse primer"
promoter complement(5540..5723)
/function="NOS promoter"
/label=NOS promoter
/note="nopaline synthase promoter"
promoter complement(5576..5755)
/label=NOS promoter
/note="nopaline synthase promoter"
misc_feature 5929..5953
/label=LB T-DNA repeat
/note="left border repeat from nopaline C58 T-DNA"
CDS 6474..7265
/codon_start=1
/gene="aadA"
/product="aminoglycoside adenylyltransferase (Murphy,
1985)"
/label=SmR
/note="confers resistance to spectinomycin and
streptomycin"
/translation="MGEAVIAEVSTQLSEVVGVIERHLEPTLLAVHLYGSAVDGGLKPH
SDIDLLVTVTVRLDETTRRALINDLLETSASPGESEILRAVEVTIVVHDDIIPWRYPAK
RELQFGEWQRNDILAGIFEPATIDIDLAILLTKAREHSVALVGPAAEELFDPVPEQDLF
EALNETLTLWNSPPDWAGDERNVVLTLSRIWYSAVTGKIAPKDVAADWAMERLPAQYQP
VILEARQAYLGQEEDRLASRADQLEEFVHYVKGEITKVVGK"
rep_origin 7511..8099
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 8000..8019
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 8253..8270
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 8285..8425
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(8351..8370)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 8511..8533
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
rep_origin 8769..8963
/label=pVS1 oriV
/note="origin of replication for the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
CDS complement(9029..10102)
/codon_start=1
/product="replication protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 RepA
/translation="MSGRKPSGPVQIGAALGDDLVEKLKAAQAAQRQRIEAEARPGESW
QAAADRIRKESRQPPAAGAPSIRKPPKGDEQPDFFVPMLYDVGTRDSRSIMDVAVFRLS
KRDRRAGEVIRYELPDGHVEVSAGPAGMASVWDYDLVLMAVSHLTESMNRYREGKGDKP
GRVFRPHVADVLKFCRRADGGKQKDDLVETCIRLNTTHVAMQRTKKAKNGRLVTVSEGE
ALISRYKIVKSETGRPEYIEIELADWMYREITEGKNPDVLTVHPDYFLIDPGIGRFLYR
LARRAAGKAEARWLFKTIYERSGSAGEFKKFCFTVRKLIGSNDLPEYDLKEEAGQAGPI
LVMRYRNLIEGEASAGS"
CDS complement(10531..11160)
/codon_start=1
/product="stability protein from the Pseudomonas plasmid
pVS1 (Heeb et al., 2000)"
/label=pVS1 StaA
/translation="MKVIAVLNQKGGSGKTTIATHLARALQLAGADVLLVDSDPQGSAR
DWAAVREDQPLTVVGIDRPTIDRDVKAIGRRDFVVIDGAPQAADLAVSAIKAADFVLIP
VQPSPYDIWATADLVELVKQRIEVTDGRLQAAFVVSRAIKGTRIGGEVAEALAGYELPI
LESRITQRVSYPGTAAAGTTVLESEPEGDAAREVQALAAEIKSKLI"
ORIGIN
1 tttcacgccc ttttaaatat ccgattattc taataaacgc tcttttctct taggtttacc
61 cgccaatata tcctgtcaaa cactgatagt ttaaactgaa ggcgggaaac gacaatctga
121 tccaagctca agctgctcta gcattcgcca ttcaggctgc gcaactgttg ggaagggcga
181 tcggtgcggg cctcttcgct attacgccag ctggcgaaag ggggatgtgc tgcaaggcga
241 ttaagttggg taacgccagg gttttcccag tcacgacgtt gtaaaacgac ggccagtgcc
301 aagcttgcat gcctgcaggt ccccagatta gccttttcaa tttcagaaag aatgctaacc
361 cacagatggt tagagaggct tacgcagcag gtctcatcaa gacgatctac ccgagcaata
421 atctccagga aatcaaatac cttcccaaga aggttaaaga tgcagtcaaa agattcagga
481 ctaactgcat caagaacaca gagaaagata tatttctcaa gatcagaagt actattccag
541 tatggacgat tcaaggcttg cttcacaaac caaggcaagt aatagagatt ggagtctcta
601 aaaaggtagt tcccactgaa tcaaaggcca tggagtcaaa gattcaaata gaggacctaa
661 cagaactcgc cgtaaagact ggcgaacagt tcatacagag tctcttacga ctcaatgaca
721 agaagaaaat cttcgtcaac atggtggagc acgacacact tgtctactcc aaaaatatca
781 aagatacagt ctcagaagac caaagggcaa ttgagacttt tcaacaaagg gtaatatccg
841 gaaacctcct cggattccat tgcccagcta tctgtcactt tattgtgaag atagtggaaa
901 aggaaggtgg ctcctacaaa tgccatcatt gcgataaagg aaaggccatc gttgaagatg
961 cctctgccga cagtggtccc aaagatggac ccccacccac gaggagcatc gtggaaaaag
1021 aagacgttcc aaccacgtct tcaaagcaag tggattgatg tgatatctcc actgacgtaa
1081 gggatgacgc acaatcccac tatccttcgc aagacccttc ctctatataa ggaagttcat
1141 ttcatttgga gagaacacgg gggactctag aggatccatg gtgagcaagg gcgaggagct
1201 gttcaccggg gtggtgccca tcctggtcga gctggacggc gacgtgaacg gccacaagtt
1261 cagcgtgtcc ggcgagggcg agggcgatgc cacctacggc aagctgaccc tgaagttcat
1321 ctgcaccacc ggcaagctgc ccgtgccctg gcccaccctc gtgaccacct tcacctacgg
1381 cgtgcagtgc ttcagccgct accccgacca catgaagcag cacgacttct tcaagtccgc
1441 catgcccgaa ggctacgtcc aggagcgcac catcttcttc aaggacgacg gcaactacaa
1501 gacccgcgcc gaggtgaagt tcgagggcga caccctggtg aaccgcatcg agctgaaggg
1561 catcgacttc aaggaggacg gcaacatcct ggggcacaag ctggagtaca actacaacag
1621 ccacaacgtc tatatcatgg ccgacaagca gaagaacggc atcaaggtga acttcaagat
1681 ccgccacaac atcgaggacg gcagcgtgca gctcgccgac cactaccagc agaacacccc
1741 catcggcgac ggccccgtgc tgctgcccga caaccactac ctgagcaccc agtccgccct
1801 gagcaaagac cccaacgaga agcgcgatca catggtcctg ctggagttcg tgaccgccgc
1861 cgggatcact cacggcatgg acgagctgta catcacaagt ttgtacaaaa aagctgaacg
1921 agaaacgtaa aatgatataa atatcaatat attaaattag attttgcata aaaaacagac
1981 tacataatac tgtaaaacac aacatatcca gtcactatgg cggcccctcg aggggccgca
2041 ttaggcaccc caggctttac actttatgct tccggctcgt ataatgtgtg gattttgagt
2101 taggatccgg cgagattttc aggagctaag gaagctaaaa tggagaaaaa aatcactgga
2161 tataccaccg ttgatatatc ccaatggcat cgtaaagaac attttgaggc atttcagtca
2221 gttgctcaat gtacctataa ccagaccgtt cagctggata ttacggcctt tttaaagacc
2281 gtaaagaaaa ataagcacaa gttttatccg gcctttattc acattcttgc ccgcctgatg
2341 aatgctcatc cggaattccg tatggcaatg aaagacggtg agctggtgat atgggatagt
2401 gttcaccctt gttacaccgt tttccatgag caaactgaaa cgttttcatc gctctggagt
2461 gaataccacg acgatttccg gcagtttcta cacatatatt cgcaagatgt ggcgtgttac
2521 ggtgaaaacc tggcctattt ccctaaaggg tttattgaga atatgttttt cgtctcagcc
2581 aatccctggg tgagtttcac cagttttgat ttaaacgtgg ccaatatgga caacttcttc
2641 gcccccgttt tcaccatggg caaatattat acgcaaggcg acaaggtgct gatgccgctg
2701 gcgattcagg ttcatcatgc cgtctgtgat ggcttccatg tcggcagaat gcttaatgaa
2761 ttacaacagt actgcgatga gtggcagggc ggggcgtaaa cgcgtggatc cggcttacta
2821 aaagccagat aacagtatgc gtatttgcgc gctgattttt gcggtataag aatatatact
2881 gatatgtata cccgaagtat gtcaaaaaga ggtgtgctat gaagcagcgt attacagtga
2941 cagttgacag cgacagctat cagttgctca aggcatatat gatgtcaata tctccggtct
3001 ggtaagcaca accatgcaga atgaagcccg tcgtctgcgt gccgaacgct ggaaagcgga
3061 aaatcaggaa gggatggctg aggtcgcccg gtttattgaa atgaacggct cttttgctga
3121 cgagaacagg gactggtgaa atgcagttta aggtttacac ctataaaaga gagagccgtt
3181 atcgtctgtt tgtggatgta cagagtgata ttattgacac gcccgggcga cggatggtga
3241 tccccctggc cagtgcacgt ctgctgtcag ataaagtctc ccgtgaactt tacccggtgg
3301 tgcatatcgg ggatgaaagc tggcgcatga tgaccaccga tatggccagt gtgccggtct
3361 ccgttatcgg ggaagaagtg gctgatctca gccaccgcga aaatgacatc aaaaacgcca
3421 ttaacctgat gttctgggga atataaatgt caggctccct tatacacagc cagtctgcag
3481 gtcgaccata gtgactggat atgttgtgtt ttacagtatt atgtagtctg ttttttatgc
3541 aaaatctaat ttaatatatt gatatttata tcattttacg tttctcgttc agctttcttg
3601 tacaaagtgg tgatgtacaa gtaaagcggc ccgagctcga atttccccga tcgttcaaac
3661 atttggcaat aaagtttctt aagattgaat cctgttgccg gtcttgcgat gattatcata
3721 taatttctgt tgaattacgt taagcatgta ataattaaca tgtaatgcat gacgttattt
3781 atgagatggg tttttatgat tagagtcccg caattataca tttaatacgc gatagaaaac
3841 aaaatatagc gcgcaaacta ggataaatta tcgcgcgcgg tgtcatctat gttactagat
3901 cgggaattgg ttccggaacc aattcgtaat catggtcata gctgtttcct gtgtgaaatt
3961 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg
4021 gtgcctaatg agtgagctaa ctcacattag gctgaattag gcgcgcctat ttctgaattc
4081 ccgatctagt aacatagatg acaccgcgcg cgataattta tcctagtttg cgcgctatat
4141 tttgttttct atcgcgtatt aaatgtataa ttgcgggact ctaatcataa aaacccatct
4201 cataaataac gtcatgcatt acatgttaat tattacatgc ttaacgtaat tcaacagaaa
4261 ttatatgata atcatcgcaa gaccggcaac aggattcaat cttaagaaac tttattgcca
4321 aatgtttgaa cgatcgggga tcatccgggt ctgtggcggg aactccacga aaatatccga
4381 acgcagcaag atatcgcggt gcatctcggt cttgcctggg cagtcgccgc cgacgccgtt
4441 gatgtggacg ccgggcccga tcatattgtc gctcaggatc gtggcgttgt gcttgtcggc
4501 cgttgctgtc gtaatgatat cggcaccttc gaccgcctgt tccgcagaga tcccgtgggc
4561 gaagaactcc agcatgagat ccccgcgctg gaggatcatc cagccggcgt cccggaaaac
4621 gattccgaag cccaaccttt catagaaggc ggcggtggaa tcgaaatctc gtgatggcag
4681 gttgggcgtc gcttggtcgg tcatttcgaa ccccagagtc ccgctcagaa gaactcgtca
4741 agaaggcgat agaaggcgat gcgctgcgaa tcgggagcgg cgataccgta aagcacgagg
4801 aagcggtcag cccattcgcc gccaagctct tcagcaatat cacgggtagc caacgctatg
4861 tcctgatagc ggtccgccac acccagccgg ccacagtcga tgaatccaga aaagcggcca
4921 ttttccacca tgatattcgg caagcaggca tcgccatggg tcacgacgag atcatcgccg
4981 tcgggcatgc gcgccttgag cctggcgaac agttcggctg gcgcgagccc ctgatgctct
5041 tcgtccagat catcctgatc gacaagaccg gcttccatcc gagtacgtgc tcgctcgatg
5101 cgatgtttcg cttggtggtc gaatgggcag gtagccggat caagcgtatg cagccgccgc
5161 attgcatcag ccatgatgga tactttctcg gcaggagcaa ggtgagatga caggagatcc
5221 tgccccggca cttcgcccaa tagcagccag tcccttcccg cttcagtgac aacgtcgagc
5281 acagctgcgc aaggaacgcc cgtcgtggcc agccacgata gccgcgctgc ctcgtcctgc
5341 agttcattca gggcaccgga caggtcggtc ttgacaaaaa gaaccgggcg cccctgcgct
5401 gacagccgga acacggcggc atcagagcag ccgattgtct gttgtgccca gtcatagccg
5461 aatagcctct ccacccaagc ggccggagaa cctgcgtgca atccatcttg ttcaatcatg
5521 cgaaacgatc cagatccggt gcagattatt tggattgaga gtgaatatga gactctaatt
5581 ggataccgag gggaatttat ggaacgtcag tggagcattt ttgacaagaa atatttgcta
5641 gctgatagtg accttaggcg acttttgaac gcgcaataat ggtttctgac gtatgtgctt
5701 agctcattaa actccagaaa cccgcggctg agtggctcct tcaacgttgc ggttctgtca
5761 gttccaaacg taaaacggct tgtcccgcgt catcggcggg ggtcataacg tgactccctt
5821 aattctccgc tcatgatgaa ttcagaaata aattcagcct aattcggcgt taattcagta
5881 cattaaaaac gtccgcaatg tgttattaag ttgtctaagc gtcaatttgt ttacaccaca
5941 atatatcctg ccaccagcca gccaacagct ccccgaccgg cagctcggca caaaatcacc
6001 actcgataca ggcagcccat cagtccggga cggcgtcagc gggagagccg ttgtaaggcg
6061 gcagactttg ctcatgttac cgatgctatt cggaagaacg gcaactaagc tgccgggttt
6121 gaaacacgga tgatctcgcg gagggtagca tgttgattgt aacgatgaca gagcgttgct
6181 gcctgtgatc aattcgggca cgaacccagt ggacataagc ctgttcggtt cgtaagctgt
6241 aatgcaagta gcgtatgcgc tcacgcaact ggtccagaac cttgaccgaa cgcagcggtg
6301 gtaacggcgc agtggcggtt ttcatggctt gttatgactg tttttttggg gtacagtcta
6361 tgcctcgggc atccaagcag caagcgcgtt acgccgtggg tcgatgtttg atgttatgga
6421 gcagcaacga tgttacgcag cagggcagtc gccctaaaac aaagttaaac atcatggggg
6481 aagcggtgat cgccgaagta tcgactcaac tatcagaggt agttggcgtc atcgagcgcc
6541 atctcgaacc gacgttgctg gccgtacatt tgtacggctc cgcagtggat ggcggcctga
6601 agccacacag tgatattgat ttgctggtta cggtgaccgt aaggcttgat gaaacaacgc
6661 ggcgagcttt gatcaacgac cttttggaaa cttcggcttc ccctggagag agcgagattc
6721 tccgcgctgt agaagtcacc attgttgtgc acgacgacat cattccgtgg cgttatccag
6781 ctaagcgcga actgcaattt ggagaatggc agcgcaatga cattcttgca ggtatcttcg
6841 agccagccac gatcgacatt gatctggcta tcttgctgac aaaagcaaga gaacatagcg
6901 ttgccttggt aggtccagcg gcggaggaac tctttgatcc ggttcctgaa caggatctat
6961 ttgaggcgct aaatgaaacc ttaacgctat ggaactcgcc gcccgactgg gctggcgatg
7021 agcgaaatgt agtgcttacg ttgtcccgca tttggtacag cgcagtaacc ggcaaaatcg
7081 cgccgaagga tgtcgctgcc gactgggcaa tggagcgcct gccggcccag tatcagcccg
7141 tcatacttga agctagacag gcttatcttg gacaagaaga agatcgcttg gcctcgcgcg
7201 cagatcagtt ggaagaattt gtccactacg tgaaaggcga gatcaccaag gtagtcggca
7261 aataatgtct agctagaaat tcgttcaagc cgacgccgct tcgcggcgcg gcttaactca
7321 agcgttagat gcactaagca cataattgct cacagccaaa ctatcaggtc aagtctgctt
7381 ttattatttt taagcgtgca taataagccc tacacaaatt gggagatata tcatgcatga
7441 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
7501 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
7561 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
7621 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
7681 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
7741 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
7801 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
7861 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
7921 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
7981 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
8041 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
8101 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
8161 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
8221 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
8281 agcgcctgat gcggtatttt ctccttacgc atctgtgcgg tatttcacac cgcatatggt
8341 gcactctcag tacaatctgc tctgatgccg catagttaag ccagtataca ctccgctatc
8401 gctacgtgac tgggtcatgg ctgcgccccg acacccgcca acacccgctg acgcgccctg
8461 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
8521 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg aggcagggtg ccttgatgtg
8581 ggcgccggcg gtcgagtggc gacggcgcgg cttgtccgcg ccctggtaga ttgcctggcc
8641 gtaggccagc catttttgag cggccagcgg ccgcgatagg ccgacgcgaa gcggcggggc
8701 gtagggagcg cagcgaccga agggtaggcg ctttttgcag ctcttcggct gtgcgctggc
8761 cagacagtta tgcacaggcc aggcgggttt taagagtttt aataagtttt aaagagtttt
8821 aggcggaaaa atcgcctttt ttctctttta tatcagtcac ttacatgtgt gaccggttcc
8881 caatgtacgg ctttgggttc ccaatgtacg ggttccggtt cccaatgtac ggctttgggt
8941 tcccaatgta cgtgctatcc acaggaaaga gaccttttcg acctttttcc cctgctaggg
9001 caatttgccc tagcatctgc tccgtacatt aggaaccggc ggatgcttcg ccctcgatca
9061 ggttgcggta gcgcatgact aggatcgggc cagcctgccc cgcctcctcc ttcaaatcgt
9121 actccggcag gtcatttgac ccgatcagct tgcgcacggt gaaacagaac ttcttgaact
9181 ctccggcgct gccactgcgt tcgtagatcg tcttgaacaa ccatctggct tctgccttgc
9241 ctgcggcgcg gcgtgccagg cggtagagaa aacggccgat gccgggatcg atcaaaaagt
9301 aatcggggtg aaccgtcagc acgtccgggt tcttgccttc tgtgatctcg cggtacatcc
9361 aatcagctag ctcgatctcg atgtactccg gccgcccggt ttcgctcttt acgatcttgt
9421 agcggctaat caaggcttca ccctcggata ccgtcaccag gcggccgttc ttggccttct
9481 tcgtacgctg catggcaacg tgcgtggtgt ttaaccgaat gcaggtttct accaggtcgt
9541 ctttctgctt tccgccatcg gctcgccggc agaacttgag tacgtccgca acgtgtggac
9601 ggaacacgcg gccgggcttg tctcccttcc cttcccggta tcggttcatg gattcggtta
9661 gatgggaaac cgccatcagt accaggtcgt aatcccacac actggccatg ccggccggcc
9721 ctgcggaaac ctctacgtgc ccgtctggaa gctcgtagcg gatcacctcg ccagctcgtc
9781 ggtcacgctt cgacagacgg aaaacggcca cgtccatgat gctgcgacta tcgcgggtgc
9841 ccacgtcata gagcatcgga acgaaaaaat ctggttgctc gtcgcccttg ggcggcttcc
9901 taatcgacgg cgcaccggct gccggcggtt gccgggattc tttgcggatt cgatcagcgg
9961 ccgcttgcca cgattcaccg gggcgtgctt ctgcctcgat gcgttgccgc tgggcggcct
10021 gcgcggcctt caacttctcc accaggtcat cacccagcgc cgcgccgatt tgtaccgggc
10081 cggatggttt gcgaccgctc acgccgattc ctcgggcttg ggggttccag tgccattgca
10141 gggccggcag acaacccagc cgcttacgcc tggccaaccg cccgttcctc cacacatggg
10201 gcattccacg gcgtcggtgc ctggttgttc ttgattttcc atgccgcctc ctttagccgc
10261 taaaattcat ctactcattt attcatttgc tcatttactc tggtagctgc gcgatgtatt
10321 cagatagcag ctcggtaatg gtcttgcctt ggcgtaccgc gtacatcttc agcttggtgt
10381 gatcctccgc cggcaactga aagttgaccc gcttcatggc tggcgtgtct gccaggctgg
10441 ccaacgttgc agccttgctg ctgcgtgcgc tcggacggcc ggcacttagc gtgtttgtgc
10501 ttttgctcat tttctcttta cctcattaac tcaaatgagt tttgatttaa tttcagcggc
10561 cagcgcctgg acctcgcggg cagcgtcgcc ctcgggttct gattcaagaa cggttgtgcc
10621 ggcggcggca gtgcctgggt agctcacgcg ctgcgtgata cgggactcaa gaatgggcag
10681 ctcgtacccg gccagcgcct cggcaacctc accgccgatg cgcgtgcctt tgatcgcccg
10741 cgacacgaca aaggccgctt gtagccttcc atccgtgacc tcaatgcgct gcttaaccag
10801 ctccaccagg tcggcggtgg cccatatgtc gtaagggctt ggctgcaccg gaatcagcac
10861 gaagtcggct gccttgatcg cggacacagc caagtccgcc gcctggggcg ctccgtcgat
10921 cactacgaag tcgcgccggc cgatggcctt cacgtcgcgg tcaatcgtcg ggcggtcgat
10981 gccgacaacg gttagcggtt gatcttcccg cacggccgcc caatcgcggg cactgccctg
11041 gggatcggaa tcgactaaca gaacatcggc cccggcgagt tgcagggcgc gggctagatg
11101 ggttgcgatg gtcgtcttgc ctgacccgcc tttctggtta agtacagcga taaccttcat
11161 gcgttcccct tgcgtatttg tttatttact catcgcatca tatacgcagc gaccgcatga
11221 cgcaagctgt tttactcaaa tacacatcac ctttttagac ggcggcgctc ggtttcttca
11281 gcggccaagc tggccggcca ggccgccagc ttggcatcag acaaaccggc caggatttca
11341 tgcagccgca cggttgagac gtgcgcgggc ggctcgaaca cgtacccggc cgcgatcatc
11401 tccgcctcga tctcttcggt aatgaaaaac ggttcgtcct ggccgtcctg gtgcggtttc
11461 atgcttgttc ctcttggcgt tcattctcgg cggccgccag ggcgtcggcc tcggtcaatg
11521 cgtcctcacg gaaggcaccg cgccgcctgg cctcggtggg cgtcacttcc tcgctgcgct
11581 caagtgcgcg gtacagggtc gagcgatgca cgccaagcag tgcagccgcc tctttcacgg
11641 tgcggccttc ctggtcgatc agctcgcggg cgtgcgcgat ctgtgccggg gtgagggtag
11701 ggcgggggcc aaacttcacg cctcgggcct tggcggcctc gcgcccgctc cgggtgcggt
11761 cgatgattag ggaacgctcg aactcggcaa tgccggcgaa cacggtcaac accatgcggc
11821 cggccggcgt ggtggtgtcg gcccacggct ctgccaggct acgcaggccc gcgccggcct
11881 cctggatgcg ctcggcaatg tccagtaggt cgcgggtgct gcgggccagg cggtctagcc
11941 tggtcactgt cacaacgtcg ccagggcgta ggtggtcaag catcctggcc agctccgggc
12001 ggtcgcgcct ggtgccggtg atcttctcgg aaaacagctt ggtgcagccg gccgcgtgca
12061 gttcggcccg ttggttggtc aagtcctggt cgtcggtgct gacgcgggca tagcccagca
12121 ggccagcggc ggcgctcttg ttcatggcgt aatgtctccg gttctagtcg caagtattct
12181 actttatgcg actaaaacac gcgacaagaa aacgccagga aaagggcagg gcggcagcct
12241 gtcgcgtaac ttaggacttg tgcgacatgt cgttttcaga agacggctgc actgaacgtc
12301 agaagccgac tgcactatag cagcggaggg gttggatcaa agtactttga tcccgagggg
12361 aaccctgtgg ttggcatgca catacaaatg gacgaacgga taaacct
//
