| 质粒名称: | pAAVLINK-3'-DYSF-FLAG |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001724 |
| 产品用途: | 3' AAVLINK plasmid for DYSF expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | Promoterless |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | DYSF |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-3'-DYSF-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-3'-DYSF-FLAG载体质粒基本信息
pAAVLINK-3'-DYSF-FLAG质粒图谱载体图谱和pAAVLINK-3'-DYSF-FLAG载体序列质粒序列多克隆位点信息
pAAVLINK-3'-DYSF-FLAG质粒载体简介
pAAVLINK-3'-DYSF-FLAG质粒序列载体序列
LOCUS pAAVLINK-3'-DYSF-FLAG 6834 bp DNA circular SYN 20-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6834)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 4, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6834
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
protein_bind 145..178
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 179..270
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 271..3573
/codon_start=1
/label=DYSF
/translation="VLPKDDIECPLGWKWEDEEWSTDLNRAVDEQGWEYSITIPPERKP
KHWVPAEKMYYTHRRRRWVRLRRRDLSQMEALKRHRQAEAEGEGWEYASLFGWKFHLEY
RKTDAFRRRRWRRRMEPLEKTGPAAVFALEGALGGVMDDKSEDSMSVSTLSFGVNRPTI
SCIFDYGNRYHLRCYMYQARDLAAMDKDSFSDPYAIVSFLHQSQKTVVVKNTLNPTWDQ
TLIFYEIEIFGEPATVAEQPPSIVVELYDHDTYGADEFMGRCICQPSLERMPRLAWFPL
TRGSQPSGELLASFELIQREKPAIHHIPGFEVQETSRILDESEDTDLPYPPPQREANIY
MVPQNIKPALQRTAIEILAWGLRNMKSYQLANISSPSLVVECGGQTVQSCVIRNLRKNP
NFDICTLFMEVMLPREELYCPPITVKVIDNRQFGRRPVVGQCTIRSLESFLCDPYSAES
PSPQGGPDDVSLLSPGEDVLIDIDDKEPLIPIQEEEFIDWWSKFFASIGEREKCGSYLE
KDFDTLKVYDTQLENVEAFEGLSDFCNTFKLYRGKTQEETEDPSVIGEFKGLFKIYPLP
EDPAIPMPPRQFHQLAAQGPQECLVRIYIVRAFGLQPKDPNGKCDPYIKISIGKKSVSD
QDNYIPCTLEPVFGKMFELTCTLPLEKDLKITLYDYDLLSKDEKIGETVVDLENRLLSK
FGARCGLPQTYCVSGPNQWRDQLRPSQLLHLFCQQHRVKAPVYRTDRVMFQDKEYSIEE
IEAGRIPNPHLGPVEERLALHVLQQQGLVPEHVESRPLYSPLQPDIEQGKLQMWVDLFP
KALGRPGPPFNITPRRARRFFLRCIIWNTRDVILDDLSLTGEKMSDIYVKGWMIGFEEH
KQKTDVHYRSLGGEGNFNWRFIFPFDYLPAEQVCTIAKKDAFWRLDKTESKIPARVVFQ
IWDNDKFSFDDFLGSLQLDLNRMPKPAKTAKKCSLDQLDDAFHPEWFVSLFEQKTVKGW
WPCVAEEGEKKILAGKLEMTLEIVAESEHEERPAGQGRDEPNMNPKLEDPRRPDTSFLW
FTSPYKTMKFILWRRFRWAIILFIILFILLLFLAIFIYAFPNYAAMKLVKPFS"
misc_feature 472..477
/label=SDS
misc_feature 1742..1747
/label=SDS
misc_feature 1931..1936
/label=SDS
misc_feature 2286..2291
/label=SDS
misc_feature 3401..3406
/label=SDS
CDS 3580..3645
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3649..4080
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3793..3798
/label=SDS
repeat_region 4097..4237
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4097..4226
/label=AAV2 ITR
rep_origin 4312..4767
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5049..5153
/gene="bla"
/label=AmpR promoter
CDS 5154..6014
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6185..6773
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtataact tcgtatagga tactttatag caatttatga
181 aactgggctt gtcgagacag agaagactct tgcgtttctg ataggcacct attggtctta
241 ctgacatcca ctttgccttt ctctccacag gtgcttccca aggatgacat tgagtgccca
301 ctgggctgga agtgggaaga tgaggaatgg tccacagacc tcaaccgggc tgtcgatgag
361 caaggctggg agtatagcat caccatcccc ccggagcgga agccgaagca ctgggtccct
421 gctgagaaga tgtactacac acaccgacgg cggcgctggg tgcgcctgcg caggagggat
481 ctcagccaaa tggaagcact gaaaaggcac aggcaggcgg aggcggaggg cgagggctgg
541 gagtacgcct ctctttttgg ctggaagttc cacctcgagt accgcaagac agatgccttc
601 cgccgccgcc gctggcgccg tcgcatggag ccactggaga agacggggcc tgcagctgtg
661 tttgcccttg agggggccct gggcggcgtg atggatgaca agagtgaaga ttccatgtcc
721 gtctccacct tgagcttcgg tgtgaacaga cccacgattt cctgcatatt cgactatggg
781 aaccgctacc atctacgctg ctacatgtac caggcccggg acctggctgc gatggacaag
841 gactcttttt ctgatcccta tgccatcgtc tccttcctgc accagagcca gaagacggtg
901 gtggtgaaga acacccttaa ccccacctgg gaccagacgc tcatcttcta cgagatcgag
961 atctttggcg agccggccac agttgctgag caaccgccca gcattgtggt ggagctgtac
1021 gaccatgaca cttatggtgc agacgagttt atgggtcgct gcatctgtca accgagtctg
1081 gaacggatgc cacggctggc ctggttccca ctgacgaggg gcagccagcc gtcgggggag
1141 ctgctggcct cttttgagct catccagaga gagaagccgg ccatccacca tattcctggt
1201 tttgaggtgc aggagacatc aaggatcctg gatgagtctg aggacacaga cctgccctac
1261 ccaccacccc agagggaggc caacatctac atggttcctc agaacatcaa gccagcgctc
1321 cagcgtaccg ccatcgagat cctggcatgg ggcctgcgga acatgaagag ttaccagctg
1381 gccaacatct cctcccccag cctcgtggta gagtgtgggg gccagacggt gcagtcctgt
1441 gtcatcagga acctccggaa gaaccccaac tttgacatct gcaccctctt catggaagtg
1501 atgctgccca gggaggagct ctactgcccc cccatcaccg tcaaggtcat cgataaccgc
1561 cagtttggcc gccggcctgt ggtgggccag tgtaccatcc gctccctgga gagcttcctg
1621 tgtgacccct actcggcgga gagtccatcc ccacagggtg gcccagacga tgtgagccta
1681 ctcagtcctg gggaagacgt gctcatcgac attgatgaca aggagcccct catccccatc
1741 caggaggaag agttcatcga ttggtggagc aaattctttg cctccatagg ggagagggaa
1801 aagtgcggct cctacctgga gaaggatttt gacaccctga aggtctatga cacacagctg
1861 gagaatgtgg aggcctttga gggcctgtct gacttttgta acaccttcaa gctgtaccgg
1921 ggcaagacgc aggaggagac agaagatcca tctgtgattg gtgaatttaa gggcctcttc
1981 aaaatttatc ccctcccaga agacccagcc atccccatgc ccccaagaca gttccaccag
2041 ctggccgccc agggacccca ggagtgcttg gtccgtatct acattgtccg agcatttggc
2101 ctgcagccca aggaccccaa tggaaagtgt gatccttaca tcaagatctc catagggaag
2161 aaatcagtga gtgaccagga taactacatc ccctgcacgc tggagcccgt atttggaaag
2221 atgttcgagc tgacctgcac tctgcctctg gagaaggacc taaagatcac tctctatgac
2281 tatgacctcc tctccaagga cgaaaagatc ggtgagacgg tcgtcgacct ggagaacagg
2341 ctgctgtcca agtttggggc tcgctgtgga ctcccacaga cctactgtgt ctctggaccg
2401 aaccagtggc gggaccagct ccgcccctcc cagctcctcc acctcttctg ccagcagcat
2461 agagtcaagg cacctgtgta ccggacagac cgtgtaatgt ttcaggataa agaatattcc
2521 attgaagaga tagaggctgg caggatccca aacccacacc tgggcccagt ggaggagcgt
2581 ctggctctgc atgtgcttca gcagcagggc ctggtcccgg agcacgtgga gtcacggccc
2641 ctctacagcc ccctgcagcc agacatcgag caggggaagc tgcagatgtg ggtcgaccta
2701 tttccgaagg ccctggggcg gcctggacct cccttcaaca tcaccccacg gagagccaga
2761 aggtttttcc tgcgttgtat tatctggaat accagagatg tgatcctgga tgacctgagc
2821 ctcacggggg agaagatgag cgacatttat gtgaaaggtt ggatgattgg ctttgaagaa
2881 cacaagcaaa agacagacgt gcattatcgt tccctgggag gtgaaggcaa cttcaactgg
2941 aggttcattt tccccttcga ctacctgcca gctgagcaag tctgtaccat tgccaagaag
3001 gatgccttct ggaggctgga caagactgag agcaaaatcc cagcacgagt ggtgttccag
3061 atctgggaca atgacaagtt ctcctttgat gattttctgg gctccctgca gctcgatctc
3121 aaccgcatgc ccaagccagc caagacagcc aagaagtgct ccttggacca gctggatgat
3181 gctttccacc cagaatggtt tgtgtccctt tttgagcaga aaacagtgaa gggctggtgg
3241 ccctgtgtag cagaagaggg tgagaagaaa atactggcgg gcaagctgga aatgaccttg
3301 gagattgtag cagagagtga gcatgaggag cggcctgctg gccagggccg ggatgagccc
3361 aacatgaacc ctaagcttga ggacccaagg cgccccgaca cctccttcct gtggtttacc
3421 tccccataca agaccatgaa gttcatcctg tggcggcgtt tccggtgggc catcatcctc
3481 ttcatcatcc tcttcatcct gctgctgttc ctggccatct tcatctacgc cttcccgaac
3541 tatgctgcca tgaagctggt gaagcccttc agctgtacag actacaaaga ccatgacggt
3601 gattataaag atcatgacat cgactacaag gatgacgatg acaagtaact cgagataatc
3661 aacctctgga ttacaaaatt tgtgaaagat tgactggtat tcttaactat gttgctcctt
3721 ttacgctatg tggatacgct gctttaatgc ctttgtatca tgctattgct tcccgtatgg
3781 ctttcatttt ctcctccttg tataaatcct ggttagttct tgccacggcg gaactcatcg
3841 ccgcctgcct tgcccgctgc tggacagggg ctcggctgtt gggcactgac aattccgtgg
3901 tgtttatttg tgaaatttgt gatgctattg ctttatttgt aaccatctag ctttatttgt
3961 gaaatttgtg atgctattgc tttatttgta accattataa gctgcaataa acaagttaac
4021 aacaacaatt gcattcattt tatgtttcag gttcaggggg agatgtggga ggttttttaa
4081 cggaccgagc ggccgcagga acccctagtg atggagttgg ccactccctc tctgcgcgct
4141 cgctcgctca ctgaggccgg gcgaccaaag gtcgcccgac gcccgggctt tgcccgggcg
4201 gcctcagtga gcgagcgagc gcgcagctgc ctgcaggggc gcctgatgcg gtattttctc
4261 cttacgcatc tgtgcggtat ttcacaccgc atacgtcaaa gcaaccatag tacgcgccct
4321 gtagcggcgc attaagcgcg gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg
4381 ccagcgcctt agcgcccgct cctttcgctt tcttcccttc ctttctcgcc acgttcgccg
4441 gctttccccg tcaagctcta aatcgggggc tccctttagg gttccgattt agtgctttac
4501 ggcacctcga ccccaaaaaa cttgatttgg gtgatggttc acgtagtggg ccatcgccct
4561 gatagacggt ttttcgccct ttgacgttgg agtccacgtt ctttaatagt ggactcttgt
4621 tccaaactgg aacaacactc aactctatct cgggctattc ttttgattta taagggattt
4681 tgccgatttc ggtctattgg ttaaaaaatg agctgattta acaaaaattt aacgcgaatt
4741 ttaacaaaat attaacgttt acaattttat ggtgcactct cagtacaatc tgctctgatg
4801 ccgcatagtt aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt
4861 gtctgctccc ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc
4921 agaggttttc accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat
4981 ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg
5041 gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc
5101 tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta
5161 ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg
5221 ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg
5281 gttacatcga actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac
5341 gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg
5401 acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt
5461 actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg
5521 ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac
5581 cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt
5641 gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag
5701 caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc
5761 aacaattaat agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc
5821 ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta
5881 tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg
5941 ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga
6001 ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac
6061 ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa
6121 tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat
6181 cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc
6241 taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg
6301 gcttcagcag agcgcagata ccaaatactg ttcttctagt gtagccgtag ttaggccacc
6361 acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg
6421 ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg
6481 ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa
6541 cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg
6601 aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga
6661 gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct
6721 gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca
6781 gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgt
//
