| 质粒名称: |
pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG
|
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001884 |
| 产品用途: | 3' AAVLINK plasmid for TET2 expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | pCALM1 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | TET2 |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG载体质粒基本信息
pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG质粒图谱载体图谱和pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG载体序列质粒序列多克隆位点信息
pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG质粒载体简介
pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG质粒序列载体序列
LOCUS pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG 6952 bp DNA circular SYN 10-AUG-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6952)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6952
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
/note="color: #ffe4c4"
promoter 145..265
/label=pCALM1
CDS 277..1308
/codon_start=1
/product="site-specific recombinase"
/label=Cre
/note="Cre is a site-specific recombinase from
bacteriophage P1. Recombination occurs at loxP sequences."
/translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
DGD"
intron 1309..1349
/label=Splicing Donor
protein_bind 1356..1389
/label=lox2272-JTZ17
/bound_moiety="Cre recombinase"
/note="Right element (RE) mutant of loxP (Araki et al.,
2010). Cre-mediated recombination occurs in the 8-bp core
sequence (GCATACAT)."
intron 1390..1481
/label=Splicing Acceptor
/note="Splicing acceptor"
CDS 1482..3665
/codon_start=1
/gene="TET2"
/gene_synonym="IMD75; KIAA1546; MDS"
/product="methylcytosine dioxygenase TET2 isoform a"
/EC_number="1.14.11.80"
/label=TET2
/note="isoform a is encoded by transcript variant
1;probable methylcytosine dioxygenase TET2; tet oncogene
family member 2; ten-eleven translocation 2"
/db_xref="GI:187761317"
/db_xref="CCDS:CCDS47120.1"
/db_xref="GeneID:54790"
/db_xref="HGNC:HGNC:25941"
/db_xref="MIM:612839"
/protein_id="NP_001120680.1"
/translation="GLDPETCGASFSFGCSWSMYYNGCKFARSKIPRKFKLLGDDPKEE
EKLESHLQNLSTLMAPTYKKLAPDAYNNQIEYEHRAPECRLGLKEGRPFSGVTACLDFC
AHAHRDLHNMQNGSTLVCTLTREDNREFGGKPEDEQLHVLPLYKVSDVDEFGSVEAQEE
KKRSGAIQVLSSFRRKVRMLAEPVKTCRQRKLEAKKAAAEKLSSLENSSNKNEKEKSAP
SRTKQTENASQAKQLAELLRLSGPVMQQSQQPQPLQKQPPQPQQQQRPQQQQPHHPQTE
SVNSYSASGSTNPYMRRPNPVSPYPNSSHTSDIYGSTSPMNFYSTSSQAAGSYLNSSNP
MNPYPGLLNQNTQYPSYQCNGNLSVDNCSPYLGSYSPQSQPMDLYRYPSQDPLSKLSLP
PIHTLYQPRFGNSQSFTSKYLGYGNQNMQGDGFSSCTIRPNVHHVGKLPPYPTHEMDGH
FMGATSRLPPNLSNPNMDYKNGEHHSPSHIIHNYSAAPGMFNSSLHALHLQNKENDMLS
HTANGLSKMLPALNHDRTACVQGGLHKLSDANGQEKQPLALVQGVASGAEDNDEVWSDS
EQSFLDPDIGGVAVAPTHGSILIECAKRELHATTPLKNPNRNHPTRISLVFYQHKSMNE
PKHGLALWEAKMAEKAREKEEECEKYGPDYVPQKSHGKKVKREPAEPHETSEPTYLRFI
KSLAERTMSVTTDSTVTTSPYAFTRVTGPYNRYI"
misc_feature 1507..1512
/label=SDS
misc_feature 1963..1968
/label=SDS
misc_feature 2823..2828
/label=SDS
misc_feature 3098..3103
/label=SDS
CDS 3672..3737
/codon_start=1
/product="three tandem FLAG(R) epitope tags, followed by an
enterokinase cleavage site"
/label=3xFLAG
/translation="DYKDHDGDYKDHDIDYKDDDDK"
terminator 3741..4172
/label=W3SL
/note="Short WPRE and SV40 polyA"
misc_feature 3885..3890
/label=SDS
repeat_region 4215..4355
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 4215..4344
/label=AAV2 ITR
repeat_region 4216..4356
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
rep_origin 4430..4885
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 5167..5271
/gene="bla"
/label=AmpR promoter
CDS 5272..6132
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 6303..6891
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtcgctct gcgcgaggcg gctccgccgc ggcggaggga
181 tacggcgcac catatatata tcgcggggcg cagactcgcg ctccggcagt ggtgctggga
241 gtgtcgtgga cgccgtgccg ttactcgtac gccaccatgt ccaatttact gaccgtacac
301 caaaatttgc ctgcattacc ggtcgatgca acgagtgatg aggttcgcaa gaacctgatg
361 gacatgttca gggatcgcca ggcgttttct gagcatacct ggaaaatgct tctgtccgtt
421 tgccggtcgt gggcggcatg gtgcaagttg aataaccgga aatggtttcc cgcagaacct
481 gaagatgttc gcgattatct tctatatctt caggcgcgcg gtctggcagt aaaaaccatc
541 cagcaacatt tgggccagct aaacatgctt catcgtcggt ccgggctgcc acgaccaagt
601 gacagcaatg ctgtttcact ggttatgcgg cggatccgaa aagaaaacgt tgatgccggt
661 gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt tcgaccaggt tcgttcactc
721 atggaaaata gcgatcgctg ccaggatata cgtaatctgg catttctggg gattgcttat
781 aacaccctgt tacgtatagc cgaaattgcc aggatcaggg ttaaagatat ctcacgtact
841 gacggtggga gaatgttaat ccatattggc agaacgaaaa cgctggttag caccgcaggt
901 gtagagaagg cacttagcct gggggtaact aaactggtcg agcgatggat ttccgtctct
961 ggtgtagctg atgatccgaa taactacctg ttttgccggg tcagaaaaaa tggtgttgcc
1021 gcgccatctg ccaccagcca gctatcaact cgcgccctgg aagggatttt tgaagcaact
1081 catcgattga tttacggcgc taaggatgac tctggtcaga gatacctggc ctggtctgga
1141 cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg ctggagtttc aataccggag
1201 atcatgcaag ctggtggctg gaccaatgta aatattgtaa tgaactatat ccgtaacctg
1261 gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg gcgattaggt aagtatcaag
1321 gttacaagac aggtttaagg agaccaataa ctagtataac ttcgtatagg atactttata
1381 gcaatttatg aaactgggct tgtcgagaca gagaagactc ttgcgtttct gataggcacc
1441 tattggtctt actgacatcc actttgcctt tctctccaca ggggctggat ccagaaacct
1501 gtggtgcctc cttctctttt ggttgttcat ggagcatgta ctacaatgga tgtaagtttg
1561 ccagaagcaa gatcccaagg aagtttaagc tgcttgggga tgacccaaaa gaggaagaga
1621 aactggagtc tcatttgcaa aacctgtcca ctcttatggc accaacatat aagaaacttg
1681 cacctgatgc atataataat cagattgaat atgaacacag agcaccagag tgccgtctgg
1741 gtctgaagga aggccgtcca ttctcagggg tcactgcatg tttggacttc tgtgctcatg
1801 cccacagaga cttgcacaac atgcagaatg gcagcacatt ggtatgcact ctcactagag
1861 aagacaatcg agaatttgga ggaaaacctg aggatgagca gcttcacgtt ctgcctttat
1921 acaaagtctc tgacgtggat gagtttggga gtgtggaagc tcaggaggag aaaaaacgga
1981 gtggtgccat tcaggtactg agttcttttc ggcgaaaagt caggatgtta gcagagccag
2041 tcaagacttg ccgacaaagg aaactagaag ccaagaaagc tgcagctgaa aagctttcct
2101 ccctggagaa cagctcaaat aaaaatgaaa aggaaaagtc agccccatca cgtacaaaac
2161 aaactgaaaa cgcaagccag gctaaacagt tggcagaact tttgcgactt tcaggaccag
2221 tcatgcagca gtcccagcag ccccagcctc tacagaagca gccaccacag ccccagcagc
2281 agcagagacc ccagcagcag cagccacatc accctcagac agagtctgtc aactcttatt
2341 ctgcttctgg atccaccaat ccatacatga gacggcccaa tccagttagt ccttatccaa
2401 actcttcaca cacttcagat atctatggaa gcaccagccc tatgaacttc tattccacct
2461 catctcaagc tgcaggttca tatttgaatt cttctaatcc catgaaccct taccctgggc
2521 ttttgaatca gaatacccaa tatccatcat atcaatgcaa tggaaaccta tcagtggaca
2581 actgctcccc atatctgggt tcctattctc cccagtctca gccgatggat ctgtataggt
2641 atccaagcca agaccctctg tctaagctca gtctaccacc catccataca ctttaccagc
2701 caaggtttgg aaatagccag agttttacat ctaaatactt aggttatgga aaccaaaata
2761 tgcagggaga tggtttcagc agttgtacca ttagaccaaa tgtacatcat gtagggaaat
2821 tgcctcctta tcccactcat gagatggatg gccacttcat gggagccacc tctagattac
2881 cacccaatct gagcaatcca aacatggact ataaaaatgg tgaacatcat tcaccttctc
2941 acataatcca taactacagt gcagctccgg gcatgttcaa cagctctctt catgccctgc
3001 atctccaaaa caaggagaat gacatgcttt cccacacagc taatgggtta tcaaagatgc
3061 ttccagctct taaccatgat agaactgctt gtgtccaagg aggcttacac aaattaagtg
3121 atgctaatgg tcaggaaaag cagccattgg cactagtcca gggtgtggct tctggtgcag
3181 aggacaacga tgaggtctgg tcagacagcg agcagagctt tctggatcct gacattgggg
3241 gagtggccgt ggctccaact catgggtcaa ttctcattga gtgtgcaaag cgtgagctgc
3301 atgccacaac ccctttaaag aatcccaata ggaatcaccc caccaggatc tccctcgtct
3361 tttaccagca taagagcatg aatgagccaa aacatggctt ggctctttgg gaagccaaaa
3421 tggctgaaaa agcccgtgag aaagaggaag agtgtgaaaa gtatggccca gactatgtgc
3481 ctcagaaatc ccatggcaaa aaagtgaaac gggagcctgc tgagccacat gaaacttcag
3541 agcccactta cctgcgtttc atcaagtctc ttgccgaaag gaccatgtcc gtgaccacag
3601 actccacagt aactacatct ccatatgcct tcactcgggt cacagggcct tacaacagat
3661 atatatgtac agactacaaa gaccatgacg gtgattataa agatcatgac atcgactaca
3721 aggatgacga tgacaagtaa ctcgagataa tcaacctctg gattacaaaa tttgtgaaag
3781 attgactggt attcttaact atgttgctcc ttttacgcta tgtggatacg ctgctttaat
3841 gcctttgtat catgctattg cttcccgtat ggctttcatt ttctcctcct tgtataaatc
3901 ctggttagtt cttgccacgg cggaactcat cgccgcctgc cttgcccgct gctggacagg
3961 ggctcggctg ttgggcactg acaattccgt ggtgtttatt tgtgaaattt gtgatgctat
4021 tgctttattt gtaaccatct agctttattt gtgaaatttg tgatgctatt gctttatttg
4081 taaccattat aagctgcaat aaacaagtta acaacaacaa ttgcattcat tttatgtttc
4141 aggttcaggg ggagatgtgg gaggtttttt aaagatctgg agaccggtgt ggcacgtgcg
4201 gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc tgcgcgctcg
4261 ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg cccgggcggc
4321 ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt attttctcct
4381 tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta cgcgccctgt
4441 agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc tacacttgcc
4501 agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac gttcgccggc
4561 tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag tgctttacgg
4621 cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc atcgccctga
4681 tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg actcttgttc
4741 caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata agggattttg
4801 ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa cgcgaatttt
4861 aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg ctctgatgcc
4921 gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg acgggcttgt
4981 ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg catgtgtcag
5041 aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat acgcctattt
5101 ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac ttttcgggga
5161 aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat gtatccgctc
5221 atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag tatgagtatt
5281 caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc tgtttttgct
5341 cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc acgagtgggt
5401 tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc cgaagaacgt
5461 tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc ccgtattgac
5521 gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt ggttgagtac
5581 tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt atgcagtgct
5641 gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat cggaggaccg
5701 aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct tgatcgttgg
5761 gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat gcctgtagca
5821 atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc ttcccggcaa
5881 caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg ctcggccctt
5941 ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc tcgcggtatc
6001 attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta cacgacgggg
6061 agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc ctcactgatt
6121 aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga tttaaaactt
6181 catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat gaccaaaatc
6241 ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat caaaggatct
6301 tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa accaccgcta
6361 ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa ggtaactggc
6421 ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt aggccaccac
6481 ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt accagtggct
6541 gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata gttaccggat
6601 aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt ggagcgaacg
6661 acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac gcttcccgaa
6721 gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga gcgcacgagg
6781 gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg ccacctctga
6841 cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa aaacgccagc
6901 aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat gt
//
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pAAVLINKv1-pCALM1-Cre-3'-TET2-FLAG质粒载体应用举例
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