| 质粒名称: | pLYL204 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002794 |
| 产品用途: | Mammalian PhCMV-driven constitutive expression vector for ecDHFRY100I-tagged NanoLuc (PhCMV-SP-ecDHFRY100I-NanoLuc-pA). |
| 存储实验室: | Mingqi XIE Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pcDNA3.1 |
| 表达系统: | 哺乳动物表达 |
| 启动子: | CMV |
| 拷贝数: | 未拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Stbl3 |
|
插入基因信息 |
|
| 插入基因名称: | ecDHFRY100I/NanoLuc |
| 物种来源: | synthetic |
| 密码子优化: | 未优化 |
pLYL204
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pLYL204载体质粒基本信息
pLYL204质粒图谱载体图谱和pLYL204载体序列质粒序列多克隆位点信息
pLYL204质粒载体简介
pLYL204质粒序列载体序列
LOCUS pLYL204 6459 bp DNA circular SYN 15-JUL-2026
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6459)
AUTHORS Westlake University
TITLE Direct Submission
JOURNAL Exported Sep 12, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6459
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 186..207
/label=olyl419
misc_feature 235..818
/label=P_CMV
/label=nonstandard type: promoter_const
misc_feature 235..614
/label=P_CMV_enhancer
/label=nonstandard type: promoter_element
misc_feature 615..818
/label=P_CMV_IE
/label=nonstandard type: promoter_element
primer_bind 769..789
/label=oMHS-CMV-F
misc_feature 829
/label=putative transcriptional start
promoter 863..882
/label=T7 promoter
primer_bind complement(871..894)
/label=olyl418
primer_bind 880..927
/label=olyl539
primer_bind 887..933
/label=oSCL1108
primer_bind 892..919
/label=olyl396
primer_bind 900..921
/label=olyl612
primer_bind 910..933
/label=oSCL084
misc_feature 912..980
/label=Secretory (IgK)
/label=nonstandard type: signal
primer_bind complement(978..1018)
/label=olyl540
primer_bind complement(978..1003)
/label=olyl500
primer_bind 987..1014
/label=olyl339
primer_bind 987..1014
/label=olyl345
primer_bind 987..1014
/label=olyl347
primer_bind complement(997..1031)
/label=olyl547
CDS 999..1475
/codon_start=1
/label=ecDHFR(Y100I)
/translation="MISLIAALAVDHVIGMENAMPWNLPADLAWFKRNTLNKPVIMGRH
TWESIGRPLPGRKNIILSSQPSTDDRVTWVKSVDEAIAACGDVPEIMVIGGGRVIEQFL
PKAQKLYLTHIDAEVEGDTHFPDYEPDDWESVFSEFHDADAQNSHSYCFEILERR"
primer_bind 999..1022
/label=olyl541
misc_feature 1002..1004
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
primer_bind 1015..1054
/label=olyl548
primer_bind complement(1037..1058)
/label=OGT61-mDHFR-R
primer_bind 1043..1067
/label=OGT61-mDHFR-2-F
primer_bind complement(1266..1304)
/label=OLYL551
primer_bind complement(1268..1298)
/label=olyl549
primer_bind 1280..1317
/label=olyl550
primer_bind complement(1286..1306)
/label=OGT62-mDHFR-R
primer_bind 1291..1311
/label=OGT62-mDHFR-2-F
primer_bind 1298..1325
/label=OLYL552
primer_bind complement(1451..1508)
/label=olyl542
primer_bind complement(1469..1493)
/label=OLYL595
CDS 1482..1493
/codon_start=1
/label=fcs
/translation="RIKR"
primer_bind 1489..1533
/label=olyl543
misc_feature 1494..1508
/label=linker3
misc_feature 1509..2018
/label=NLuc (nano Luciferase)
/label=nonstandard type: reporter
misc_feature 1539..1541
/label=cyt-STOP (TGG->TGA)
primer_bind complement(1998..2018)
/label=olyl592
primer_bind complement(2000..2041)
/label=olyl544
primer_bind complement(2004..2018)
/label=olyl459
primer_bind complement(2008..2018)
/label=oSCL085
primer_bind complement(2008..2018)
/label=oSCL1109
terminator 2056..2283
/label=bGH polyA
rep_origin 2329..2757
/label=f1 bacteriophage origin of replication
/label=f1 bacteriophage origin of replication; arrow _f1
ori
misc_feature 2771..3100
/label=P_SV40
/label=nonstandard type: promoter_const
rep_origin 2951..3086
/label=SV40 ORI
misc_feature 3003..3052
/label=P_SV40 TSS-region
CDS 3167..3961
/label=Translation 2136-2930
misc_feature 3167..3958
/label=Kan/neoR
/label=nonstandard type: resistance
primer_bind complement(4301..4321)
/label=M13-rev
misc_binding complement(4327..4349)
/label=LacO
promoter complement(4353..4383)
/label=lac promoter
rep_origin complement(4707..5292)
/direction=LEFT
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
/label=high-copy-number ColE1/pMB1/pBR322/pUC origin of
_ori
CDS complement(5463..6323)
/label=Translation 5292-4432
misc_feature complement(5466..6323)
/label=AmpR
/label=nonstandard type: resistance
promoter complement(6324..6428)
/label=AmpR promoter
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctaga
901 agcttgccac catgactagt gagacagaca cactcctgct atgggtactg ctgctctggg
961 ttccaggttc cactggtgac gctagtggtg gttctggtat gatcagcctg atcgccgccc
1021 tggccgtgga ccacgtgatc ggcatggaga acgcgatgcc ctggaacctg cccgccgacc
1081 tggcctggtt caagagaaac accctgaaca agcccgtgat catgggcaga cacacctggg
1141 agagcatcgg cagacccctg cccggcagaa agaacatcat cctgagcagc cagcccagca
1201 ccgacgacag agtgacctgg gtgaagagcg tggacgaggc catcgccgcc tgcggcgacg
1261 tgcccgagat catggtgatc ggcggcggca gagtgatcga gcagttcctg cccaaggccc
1321 agaagctgta cctgacccac atcgacgccg aggtggaggg cgacacccac ttccccgact
1381 acgagcccga cgactgggag agcgtgttca gcgagttcca cgacgccgac gcccagaaca
1441 gccacagcta ctgcttcgag atcctggaga gaagagctag caggatcaag aggggttcct
1501 ctagcggagt cttcacactc gaagatttcg ttggggactg gcgacagaca gccggctaca
1561 acctggacca agtccttgaa cagggaggtg tgtccagttt gtttcagaat ctcggggtgt
1621 ccgtaactcc gatccaaagg attgtcctga gcggtgaaaa tgggctgaag atcgacatcc
1681 atgtcatcat cccgtatgaa ggtctgagcg gcgaccaaat gggccagatc gaaaaaattt
1741 ttaaggtggt gtaccctgtg gatgatcatc actttaaggt gatcctgcac tatggcacac
1801 tggtaatcga cggggttacg ccgaacatga tcgactattt cggacggccg tatgaaggca
1861 tcgccgtgtt cgacggcaaa aagatcactg taacagggac cctgtggaac ggcaacaaaa
1921 ttatcgacga gcgcctgatc aaccccgacg gctccctgct gttccgagta accatcaacg
1981 gagtgaccgg ctggcggctg tgcgaacgca ttctggcgta atctagaggg cccgtttaaa
2041 cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt tgcccctccc
2101 ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa taaaatgagg
2161 aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg gtggggcagg
2221 acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg gtgggctcta
2281 tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac gcgccctgta
2341 gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct acacttgcca
2401 gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg ttcgccggct
2461 ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt gctttacggc
2521 acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca tcgccctgat
2581 agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga ctcttgttcc
2641 aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa gggattttgc
2701 cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac gcgaattaat
2761 tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag caggcagaag
2821 tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc caggctcccc
2881 agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag tcccgcccct
2941 aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc cccatggctg
3001 actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc tattccagaa
3061 gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg gagcttgtat
3121 atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga ttgaacaaga
3181 tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct atgactgggc
3241 acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc aggggcgccc
3301 ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg acgaggcagc
3361 gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg acgttgtcac
3421 tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc tcctgtcatc
3481 tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc ggctgcatac
3541 gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg agcgagcacg
3601 tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc atcaggggct
3661 cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg aggatctcgt
3721 cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc gcttttctgg
3781 attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag cgttggctac
3841 ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg tgctttacgg
3901 tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg agttcttctg
3961 agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc atcacgagat
4021 ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc
4081 ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg
4141 tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa
4201 gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat
4261 gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct
4321 gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt
4381 aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc
4441 gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg
4501 agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg
4561 gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca
4621 gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac
4681 cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac
4741 aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg
4801 tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac
4861 ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg ctgtaggtat
4921 ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag
4981 cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac
5041 ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt
5101 gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac agtatttggt
5161 atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc
5221 aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac gcgcagaaaa
5281 aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
5341 aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
5401 ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
5461 agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
5521 atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
5581 cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
5641 aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
5701 cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
5761 aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
5821 ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
5881 gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
5941 ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
6001 tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
6061 tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
6121 ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
6181 tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
6241 agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
6301 acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
6361 ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
6421 gttccgcgca catttccccg aaaagtgcca cctgacgtc
//
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