pLYL205

LOCUS      pLYL205              6256 bp DNA     circular SYN 15-JUL-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6256)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 12, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6256
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     186..207
                     /label=olyl419
     misc_feature    235..818
                     /label=P_CMV
                     /label=nonstandard type: promoter_const
     misc_feature    235..614
                     /label=P_CMV_enhancer
                     /label=nonstandard type: promoter_element
     misc_feature    615..818
                     /label=P_CMV_IE
                     /label=nonstandard type: promoter_element
     primer_bind     769..789
                     /label=oMHS-CMV-F
     misc_feature    829
                     /label=putative transcriptional start
     promoter        863..882
                     /label=T7 promoter
     primer_bind     complement(871..894)
                     /label=olyl418
     primer_bind     881..974
                     /label=oSCL1213
     primer_bind     881..939
                     /label=oSCL1369
     primer_bind     881..900
                     /label=olyl553
     primer_bind     881..882
                     /label=T7
     misc_feature    910..957
                     /label=Signal peptide-SEAP
     primer_bind     910..924
                     /label=oSCL1368
     primer_bind     913..927
                     /label=oSCL1368
     primer_bind     916..974
                     /label=oSCL1368
     primer_bind     complement(936..957)
                     /label=Primer 1
     misc_feature    958..1278
                     /label=DD
     primer_bind     complement(1044..1085)
                     /label=oSCL1214
     primer_bind     1066..1085
                     /label=oSCL1215
     primer_bind     complement(1251..1295)
                     /label=oSCL1216
     primer_bind     complement(1258..1273)
                     /label=Oscl18-3
     primer_bind     complement(1260..1295)
                     /label=Oscl18-2
     primer_bind     complement(1264..1284)
                     /label=olyl554
     primer_bind     1265..1311
                     /label=olyl555
     misc_feature    1279..1284
                     /label=linker
     misc_feature    1284
                     /label=Secretory (IgK)
                     /label=nonstandard type:  signal
     misc_feature    1306..1815
                     /label=NLuc (nano Luciferase)
                     /label=nonstandard type:  reporter
     primer_bind     1306..1330
                     /label=olyl543
     misc_feature    1336..1338
                     /label=cyt-STOP (TGG->TGA)
     primer_bind     complement(1797..1838)
                     /label=olyl544
     primer_bind     complement(1797..1838)
                     /label=olyl556
     primer_bind     complement(1801..1815)
                     /label=olyl459
     primer_bind     complement(1805..1815)
                     /label=oSCL085
     primer_bind     complement(1805..1815)
                     /label=oSCL1109
     terminator      1853..2080
                     /label=bGH polyA
     rep_origin      2126..2554
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     misc_feature    2568..2897
                     /label=P_SV40
                     /label=nonstandard type: promoter_const
     rep_origin      2748..2883
                     /label=SV40 ORI
     misc_feature    2800..2849
                     /label=P_SV40 TSS-region
     CDS             2964..3758
                     /label=Translation 2136-2930
     misc_feature    2964..3755
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     primer_bind     complement(4098..4118)
                     /label=M13-rev
     misc_binding    complement(4124..4146)
                     /label=LacO
     promoter        complement(4150..4180)
                     /label=lac promoter
     rep_origin      complement(4504..5089)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     _ori
     CDS             complement(5260..6120)
                     /label=Translation 5292-4432
     misc_feature    complement(5263..6120)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(6121..6225)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 gccaccatgc tgctgctgct gctgctgctg ggcctgaggc tacagctctc cctgggcggc
      961 gttcaggttg aaacaattag cccgggagat ggccgtacat ttccgaaacg tggccagaca
     1021 tgtgttgttc actataccgg catgctggag gatggtaaaa aagtggatag cagccgtgac
     1081 cgtaataaac cgttcaaatt catgctgggc aaacaggaag tcattcgcgg ttgggaagaa
     1141 ggtgtcgcac agatgagtgt gggtcaacgt gccaaactga cgattagtcc tgactatgcc
     1201 tatggagcaa caggtcatcc agggattatt ccgcctcacg ctactctggt ctttgatgtg
     1261 gaactgctga aacccgaagc cagcgctagt ggtggttctg gtatggtctt cacactcgaa
     1321 gatttcgttg gggactggcg acagacagcc ggctacaacc tggaccaagt ccttgaacag
     1381 ggaggtgtgt ccagtttgtt tcagaatctc ggggtgtccg taactccgat ccaaaggatt
     1441 gtcctgagcg gtgaaaatgg gctgaagatc gacatccatg tcatcatccc gtatgaaggt
     1501 ctgagcggcg accaaatggg ccagatcgaa aaaattttta aggtggtgta ccctgtggat
     1561 gatcatcact ttaaggtgat cctgcactat ggcacactgg taatcgacgg ggttacgccg
     1621 aacatgatcg actatttcgg acggccgtat gaaggcatcg ccgtgttcga cggcaaaaag
     1681 atcactgtaa cagggaccct gtggaacggc aacaaaatta tcgacgagcg cctgatcaac
     1741 cccgacggct ccctgctgtt ccgagtaacc atcaacggag tgaccggctg gcggctgtgc
     1801 gaacgcattc tggcgtaatc tagagggccc gtttaaaccc gctgatcagc ctcgactgtg
     1861 ccttctagtt gccagccatc tgttgtttgc ccctcccccg tgccttcctt gaccctggaa
     1921 ggtgccactc ccactgtcct ttcctaataa aatgaggaaa ttgcatcgca ttgtctgagt
     1981 aggtgtcatt ctattctggg gggtggggtg gggcaggaca gcaaggggga ggattgggaa
     2041 gacaatagca ggcatgctgg ggatgcggtg ggctctatgg cttctgaggc ggaaagaacc
     2101 agctggggct ctagggggta tccccacgcg ccctgtagcg gcgcattaag cgcggcgggt
     2161 gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc cgctcctttc
     2221 gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc tctaaatcgg
     2281 gggctccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa aaaacttgat
     2341 tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg ccctttgacg
     2401 ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac actcaaccct
     2461 atctcggtct attcttttga tttataaggg attttgccga tttcggccta ttggttaaaa
     2521 aatgagctga tttaacaaaa atttaacgcg aattaattct gtggaatgtg tgtcagttag
     2581 ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     2641 agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca
     2701 tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc ccgcccctaa
     2761 ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt atttatgcag
     2821 aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc ttttttggag
     2881 gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga tctgatcaag
     2941 agacaggatg aggatcgttt cgcatgattg aacaagatgg attgcacgca ggttctccgg
     3001 ccgcttgggt ggagaggcta ttcggctatg actgggcaca acagacaatc ggctgctctg
     3061 atgccgccgt gttccggctg tcagcgcagg ggcgcccggt tctttttgtc aagaccgacc
     3121 tgtccggtgc cctgaatgaa ctgcaggacg aggcagcgcg gctatcgtgg ctggccacga
     3181 cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga agcgggaagg gactggctgc
     3241 tattgggcga agtgccgggg caggatctcc tgtcatctca ccttgctcct gccgagaaag
     3301 tatccatcat ggctgatgca atgcggcggc tgcatacgct tgatccggct acctgcccat
     3361 tcgaccacca agcgaaacat cgcatcgagc gagcacgtac tcggatggaa gccggtcttg
     3421 tcgatcagga tgatctggac gaagagcatc aggggctcgc gccagccgaa ctgttcgcca
     3481 ggctcaaggc gcgcatgccc gacggcgagg atctcgtcgt gacccatggc gatgcctgct
     3541 tgccgaatat catggtggaa aatggccgct tttctggatt catcgactgt ggccggctgg
     3601 gtgtggcgga ccgctatcag gacatagcgt tggctacccg tgatattgct gaagagcttg
     3661 gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat cgccgctccc gattcgcagc
     3721 gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc gggactctgg ggttcgaaat
     3781 gaccgaccaa gcgacgccca acctgccatc acgagatttc gattccaccg ccgccttcta
     3841 tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc tggatgatcc tccagcgcgg
     3901 ggatctcatg ctggagttct tcgcccaccc caacttgttt attgcagctt ataatggtta
     3961 caaataaagc aatagcatca caaatttcac aaataaagca tttttttcac tgcattctag
     4021 ttgtggtttg tccaaactca tcaatgtatc ttatcatgtc tgtataccgt cgacctctag
     4081 ctagagcttg gcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt atccgctcac
     4141 aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg cctaatgagt
     4201 gagctaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg gaaacctgtc
     4261 gtgccagctg cattaatgaa tcggccaacg cgcggggaga ggcggtttgc gtattgggcg
     4321 ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     4381 atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     4441 gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     4501 gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     4561 gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     4621 gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     4681 aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     4741 ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     4801 taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagccac
     4861 tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct tgaagtggtg
     4921 gcctaactac ggctacacta gaagaacagt atttggtatc tgcgctctgc tgaagccagt
     4981 taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg ctggtagcgg
     5041 tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag aagatccttt
     5101 gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag ggattttggt
     5161 catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat gaagttttaa
     5221 atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct taatcagtga
     5281 ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac tccccgtcgt
     5341 gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa tgataccgcg
     5401 agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg gaagggccga
     5461 gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt gttgccggga
     5521 agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca ttgctacagg
     5581 catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt cccaacgatc
     5641 aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct tcggtcctcc
     5701 gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg cagcactgca
     5761 taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg agtactcaac
     5821 caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg cgtcaatacg
     5881 ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa aacgttcttc
     5941 ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt aacccactcg
     6001 tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt gagcaaaaac
     6061 aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt gaatactcat
     6121 actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca tgagcggata
     6181 catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat ttccccgaaa
     6241 agtgccacct gacgtc
//