| 质粒名称: | pET-hisTdT |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0002249 |
| 产品用途: | active his-tagged N-terminus truncated TdT |
| 存储实验室: | Po-Min Chiang Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pET3c |
| 表达系统: | 细菌表达 |
| 启动子: | T7 |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | Neb stable |
|
插入基因信息 |
|
| 插入基因名称: | TdT |
| 物种来源: | Bos taurus |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
USPPAR is a cost-effective, scalable, and highly sensitive single-cell RNA sequencing workflow compatible with diverse specimens.
Hsueh, Y.-W. & Chiang, P.-M.
PLOS Biol. 23, e3003537 (2025)
|
买家导航
pET-hisTdT载体质粒基本信息
pET-hisTdT质粒图谱载体图谱和pET-hisTdT载体序列质粒序列多克隆位点信息
pET-hisTdT质粒载体简介
pET-hisTdT质粒序列载体序列
LOCUS pET-hisTdT 5781 bp DNA circular SYN 17-APR-2026
DEFINITION Expresses his-tagged N-terminus truncated TdT in E. coli hosts.
ACCESSION .
VERSION .
KEYWORDS pET-hisTdT
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 5781)
TITLE Bacterial expression plasmids for production of recombinant PNK
JOURNAL Unpublished
REFERENCE 2 (bases 1 to 5781)
AUTHORS .
TITLE Direct Submission
JOURNAL Exported Apr 21, 2026 from SnapGene 6.0.2
https://www.biofeng.com
COMMENT SGRef: number: 1; type: "Journal Article"; journalName:
"Unpublished"
FEATURES Location/Qualifiers
source 1..5781
/mol_type="other DNA"
/organism="synthetic DNA construct"
primer_bind 1..20
/label=T7
/note="T7 promoter, forward primer"
promoter 1..19
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
RBS 51..73
/note="efficient ribosome binding site from bacteriophage
T7 gene 10 (Olins and Rangwala, 1989)"
CDS 84..113
/codon_start=1
/product="10xHis affinity tag"
/label=10xHis
/translation="HHHHHHHHHH"
primer_bind complement(1311..1329)
/label=T7 Term
/note="T7 terminator, reverse primer"
terminator 1325..1372
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
promoter complement(1738..1766)
/label=tet promoter
/note="E. coli promoter for tetracycline efflux protein
gene"
primer_bind complement(1793..1811)
/label=pBRforEco
/note="pBR322 vectors, upsteam of EcoRI site, forward
primer"
promoter 1879..1983
/gene="bla"
/label=AmpR promoter
CDS 1984..2844
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
primer_bind complement(2202..2221)
/label=Amp-R
/note="Ampicillin resistance gene, reverse primer"
rep_origin 3015..3603
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
primer_bind 3504..3523
/label=pBR322ori-F
/note="pBR322 origin, forward primer"
primer_bind 3757..3774
/label=L4440
/note="L4440 vector, forward primer"
misc_feature 3789..3928
/label=bom
/note="basis of mobility region from pBR322"
primer_bind complement(3854..3873)
/label=pRS-marker
/note="pRS vectors, use to sequence yeast selectable
marker"
primer_bind 4014..4036
/label=pGEX 3'
/note="pGEX vectors, reverse primer"
CDS complement(4030..4221)
/codon_start=1
/gene="rop"
/product="Rop protein, which maintains plasmids at low copy
number"
/label=rop
/translation="MTKQEKTALNMARFIRSQTLTLLEKLNELDADEQADICESLHDHA
DELYRSCLARFGDDGENL"
primer_bind 5679..5698
/label=pBRrevBam
/note="pBR322 vectors, tet region, downstream of BamHI,
reverse primer"
ORIGIN
1 taatacgact cactataggg agaccacaac ggtttccctc tagaaataat tttgtttaac
61 tttaagaagg agatatacat atgcaccatc atcatcatca tcaccaccac catatgcgta
121 ccgattacag cgcgaccccg aacccgggct ttcagaaaac cccgccgctg gcggtgaaaa
181 aaatcagcca gtacgcgtgt cagcgcaaaa ccaccctgaa caactataac cacattttta
241 ccgatgcgtt tgaaattctg gcggaaaaca gcgaatttaa agaaaacgaa gtgagctatg
301 tgacctttat gcgcgcggcc agcgtgctga aaagcctgcc gtttaccatt atctccatga
361 aagataccga aggcatcccg tgtctgggcg ataaagtgaa atgtattatt gaagaaatta
421 ttgaagatgg cgaatcatca gaagtgaaag ccgtgctgaa cgatgaacgc tatcagagct
481 ttaaattatt taccagcgtg tttggcgtgg gtctgaaaac ctcagaaaaa tggtttcgca
541 tgggttttcg cagcctgtcc aaaattatgt ctgacaaaac gctgaaattt acgaaaatgc
601 agaaagctgg ttttctgtat tatgaagacc tggtatcgtg tgtaacccgc gctgaagctg
661 aagccgtagg tgtactcgta aaagaagctg tttgggcttt tctgccggat gcctttgtta
721 caatgacagg tggctttcgc cgtggcaaaa aaatcggcca cgatgttgat tttctgatca
781 cctccccggg ttccgccgaa gatgaagaac agcttttacc gaaagtgatc aatctctggg
841 aaaaaaaagg tctgctgctc tattatgatc tagtggaatc gacctttgaa aaattcaaac
901 tcccgagtcg gcaggtggat acgctggatc atttccagaa atgtttcttg atcctgaaac
961 tacatcatca gcgtgtggat agtagcaaaa gcaatcagca ggaaggtaaa acatggaaag
1021 ccatccgtgt tgatttggtg atgtgtccgt atgaaaatcg tgccttcgcc ctgctgggct
1081 ggaccggctc ccgtcagttc gaacgtgata ttcgtcgcta tgcgacccat gaacgtaaaa
1141 tgatgctgga taatcatgcg ctgtatgata aaaccaaacg tgtgtttctg aaagcggaaa
1201 gcgaagaaga aatttttgcg catctgggcc tggattatat tgaaccgtgg gaacgtaatg
1261 cgtaacggct gctaacaaag cccgaaagga agctgagttg gctgctgcca ccgctgagca
1321 ataactagca taaccccttg gggcctctaa acgggtcttg aggggttttt tgctgaaagg
1381 aggaactata tccggatatc cacaggacgg gtgtggtcgc catgatcgcg tagtcgatag
1441 tggctccaag tagcgaagcg agcaggactg ggcggcggcc aaagcggtcg gacagtgctc
1501 cgagaacggg tgcgcataga aattgcatca acgcatatag cgctagcagc acgccatagt
1561 gactggcgat gctgtcggaa tggacgatat cccgcaagag gcccggcagt accggcataa
1621 ccaagcctat gcctacagca tccagggtga cggtgccgag gatgacgatg agcgcattgt
1681 tagatttcat acacggtgcc tgactgcgtt agcaatttaa ctgtgataaa ctaccgcatt
1741 aaagcttatc gatgataagc tgtcaaacat gagaattctt gaagacgaaa gggcctcgtg
1801 atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
1861 acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
1921 atgtatccgc tcatgagaca ataaccctga taaatgcttc aataatattg aaaaaggaag
1981 agtatgagta ttcaacattt ccgtgtcgcc cttattccct tttttgcggc attttgcctt
2041 cctgtttttg ctcacccaga aacgctggtg aaagtaaaag atgctgaaga tcagttgggt
2101 gcacgagtgg gttacatcga actggatctc aacagcggta agatccttga gagttttcgc
2161 cccgaagaac gttttccaat gatgagcact tttaaagttc tgctatgtgg cgcggtatta
2221 tcccgtgttg acgccgggca agagcaactc ggtcgccgca tacactattc tcagaatgac
2281 ttggttgagt actcaccagt cacagaaaag catcttacgg atggcatgac agtaagagaa
2341 ttatgcagtg ctgccataac catgagtgat aacactgcgg ccaacttact tctgacaacg
2401 atcggaggac cgaaggagct aaccgctttt ttgcacaaca tgggggatca tgtaactcgc
2461 cttgatcgtt gggaaccgga gctgaatgaa gccataccaa acgacgagcg tgacaccacg
2521 atgcctgcag caatggcaac aacgttgcgc aaactattaa ctggcgaact acttactcta
2581 gcttcccggc aacaattaat agactggatg gaggcggata aagttgcagg accacttctg
2641 cgctcggccc ttccggctgg ctggtttatt gctgataaat ctggagccgg tgagcgtggg
2701 tctcgcggta tcattgcagc actggggcca gatggtaagc cctcccgtat cgtagttatc
2761 tacacgacgg ggagtcaggc aactatggat gaacgaaata gacagatcgc tgagataggt
2821 gcctcactga ttaagcattg gtaactgtca gaccaagttt actcatatat actttagatt
2881 gatttaaaac ttcattttta atttaaaagg atctaggtga agatcctttt tgataatctc
2941 atgaccaaaa tcccttaacg tgagttttcg ttccactgag cgtcagaccc cgtagaaaag
3001 atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa
3061 aaaccaccgc taccagcggt ggtttgtttg ccggatcaag agctaccaac tctttttccg
3121 aaggtaactg gcttcagcag agcgcagata ccaaatactg tccttctagt gtagccgtag
3181 ttaggccacc acttcaagaa ctctgtagca ccgcctacat acctcgctct gctaatcctg
3241 ttaccagtgg ctgctgccag tggcgataag tcgtgtctta ccgggttgga ctcaagacga
3301 tagttaccgg ataaggcgca gcggtcgggc tgaacggggg gttcgtgcac acagcccagc
3361 ttggagcgaa cgacctacac cgaactgaga tacctacagc gtgagctatg agaaagcgcc
3421 acgcttcccg aagggagaaa ggcggacagg tatccggtaa gcggcagggt cggaacagga
3481 gagcgcacga gggagcttcc agggggaaac gcctggtatc tttatagtcc tgtcgggttt
3541 cgccacctct gacttgagcg tcgatttttg tgatgctcgt caggggggcg gagcctatgg
3601 aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct tttgctggcc ttttgctcac
3661 atgttctttc ctgcgttatc ccctgattct gtggataacc gtattaccgc ctttgagtga
3721 gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg
3781 gaagagcgcc tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcaat
3841 ggtgcactct cagtacaatc tgctctgatg ccgcatagtt aagccagtat acactccgct
3901 atcgctacgt gactgggtca tggctgcgcc ccgacacccg ccaacacccg ctgacgcgcc
3961 ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg tctccgggag
4021 ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgaggcagc tgcggtaaag
4081 ctcatcagcg tggtcgtgaa gcgattcaca gatgtctgcc tgttcatccg cgtccagctc
4141 gttgagtttc tccagaagcg ttaatgtctg gcttctgata aagcgggcca tgttaagggc
4201 ggttttttcc tgtttggtca ctgatgcctc cgtgtaaggg ggatttctgt tcatgggggt
4261 aatgataccg atgaaacgag agaggatgct cacgatacgg gttactgatg atgaacatgc
4321 ccggttactg gaacgttgtg agggtaaaca actggcggta tggatgcggc gggaccagag
4381 aaaaatcact cagggtcaat gccagcgctt cgttaataca gatgtaggtg ttccacaggg
4441 tagccagcag catcctgcga tgcagatccg gaacataatg gtgcagggcg ctgacttccg
4501 cgtttccaga ctttacgaaa cacggaaacc gaagaccatt catgttgttg ctcaggtcgc
4561 agacgttttg cagcagcagt cgcttcacgt tcgctcgcgt atcggtgatt cattctgcta
4621 accagtaagg caaccccgcc agcctagccg ggtcctcaac gacaggagca cgatcatgcg
4681 cacccgtggc caggacccaa cgctgcccga gatgcgccgc gtgcggctgc tggagatggc
4741 ggacgcgatg gatatgttct gccaagggtt ggtttgcgca ttcacagttc tccgcaagaa
4801 ttgattggct ccaattcttg gagtggtgaa tccgttagcg aggtgccgcc ggcttccatt
4861 caggtcgagg tggcccggct ccatgcaccg cgacgcaacg cggggaggca gacaaggtat
4921 agggcggcgc ctacaatcca tgccaacccg ttccatgtgc tcgccgaggc ggcataaatc
4981 gccgtgacga tcagcggtcc aatgatcgaa gttaggctgg taagagccgc gagcgatcct
5041 tgaagctgtc cctgatggtc gtcatctacc tgcctggaca gcatggcctg caacgcgggc
5101 atcccgatgc cgccggaagc gagaagaatc ataatgggga aggccatcca gcctcgcgtc
5161 gcgaacgcca gcaagacgta gcccagcgcg tcggccgcca tgccggcgat aatggcctgc
5221 ttctcgccga aacgtttggt ggcgggacca gtgacgaagg cttgagcgag ggcgtgcaag
5281 attccgaata ccgcaagcga caggccgatc atcgtcgcgc tccagcgaaa gcggtcctcg
5341 ccgaaaatga cccagagcgc tgccggcacc tgtcctacga gttgcatgat aaagaagaca
5401 gtcataagtg cggcgacgat agtcatgccc cgcgcccacc ggaaggagct gactgggttg
5461 aaggctctca agggcatcgg tcgacgctct cccttatgcg actcctgcat taggaagcag
5521 cccagtagta ggttgaggcc gttgagcacc gccgccgcaa ggaatggtgc atgcaaggag
5581 atggcgccca acagtccccc ggccacgggg cctgccacca tacccacgcc gaaacaagcg
5641 ctcatgagcc cgaagtggcg agcccgatct tccccatcgg tgatgtcggc gatataggcg
5701 ccagcaaccg cacctgtggc gccggtgatg ccggccacga tgcgtccggc gtagaggatc
5761 gagatctcga tcccgcgaaa t
//
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