| 质粒名称: | pGEX-6p-1-AT5G60980 |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0000925 |
| 产品用途: | Express proteins in vitro |
| 存储实验室: | Peiguo YANG Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pGEX-6p-1 |
| 表达系统: | 细菌表达 |
| 启动子: | tac, AmpR, Lac, LacIq |
| 拷贝数: | 低拷贝 |
| 载体抗性: | 氨苄霉素氯霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha, RosettaDE3, BL21 |
|
插入基因信息 |
|
| 插入基因名称: | AT5G60980 |
| 物种来源: | Arabidopsis thaliana |
| 密码子优化: | 细菌表达优化 |
|
文献引用方法 |
|
| 参考文献部分: |
The divergent effects of G3BP orthologs on human stress granule assembly imply a centric role for the core protein interaction network
Yao Z., Liu Y., Chen Q., Chen X., Zhu Z., Song S., Ma X., Yang P.
Cell Rep. 43, 114617 (2024)
|
买家导航
pGEX-6p-1-AT5G60980载体质粒基本信息
pGEX-6p-1-AT5G60980质粒图谱载体图谱和pGEX-6p-1-AT5G60980载体序列质粒序列多克隆位点信息
pGEX-6p-1-AT5G60980质粒载体简介
pGEX-6p-1-AT5G60980质粒序列载体序列
LOCUS pGEX-6p-1-AT5G60980 6376 bp DNA circular SYN 09-DEC-2024
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6376)
AUTHORS Triple Threat
TITLE Direct Submission
JOURNAL Exported Apr 16, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6376
/mol_type="other DNA"
/organism="synthetic DNA construct"
promoter 183..211
/label=tac promoter
/note="strong E. coli promoter; hybrid between the trp and
lac UV5 promoters"
protein_bind 219..235
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
CDS 258..911
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/label=GST
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 918..941
/codon_start=1
/product="recognition and cleavage site for human
rhinovirus 3C and PreScission proteases"
/label=HRV 3C site
/translation="LEVLFQGP"
CDS 951..971
/codon_start=1
/product="tobacco etch virus (TEV) protease recognition and
cleavage site"
/label=TEV site
/translation="ENLYFQG"
CDS 978..2357
/codon_start=1
/label=AT5G3BP1 (60980)
/translation="MAQQEASPSPGAEVVGRAFVEQYYHILHQSPGLVHRFYQDSSFLT
RPDVTGAVTTVTTMQAINDKILSLKYEDYTAEIETADAQESHERGVIVLVTGRLTGNDN
VRKKFSQSFFLAPQDKGYFVLNDVFRFLEEKEVTAQARSVPINGTTRDVQAPIEPERVV
VSHEPEVEPEPVASIEEEDLDNVAEVYDPSDKDEGVVVDVEPIEPPTQISHNEILSVPQ
GDAPKHSYASILKQMKSSPAPTTHVARNKPRPAPVNQKLTAPPAEPAARPEASAHENVP
NSSHVDVEDDGHSIYVRNLPFDSTPTQLEEVFKNFGAIKHEGIQVRSNKQQGFCFGFVE
FETSSGKQSALEASPVTIGDRQAVVEEKKTNSRGGGNNGGSRGRYFSGRGSFRNESFKG
GRGGGGRGGYGRGGGEFSGRPKSSNPRNGGEGYQRVPQNGGGGRGGRGEGGRGGARGGG
SS"
misc_feature complement(2340..2357)
/label=Primer R
promoter 2679..2783
/gene="bla"
/label=AmpR promoter
CDS 2784..3644
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 3815..4403
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
promoter 4647..4724
/gene="lacI (mutant)"
/label=lacIq promoter
/note="In the lacIq allele, a single base change in the
promoter boosts expression of the lacI gene about 10-fold."
CDS 4725..5807
/codon_start=1
/gene="lacI"
/product="lac repressor"
/label=lacI
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
/translation="MKPVTLYDVAEYAGVSYQTVSRVVNQASHVSAKTREKVEAAMAEL
NYIPNRVAQQLAGKQSLLIGVATSSLALHAPSQIVAAIKSRADQLGASVVVSMVERSGV
EACKAAVHNLLAQRVSGLIINYPLDDQDAIAVEAACTNVPALFLDVSDQTPINSIIFSH
EDGTRLGVEHLVALGHQQIALLAGPLSSVSARLRLAGWHKYLTRNQIQPIAEREGDWSA
MSGFQQTMQMLNEGIVPTAMLVANDQMALGAMRAITESGLRVGADISVVGYDDTEDSSC
YIPPSTTIKQDFRLLGQTSVDRLLQLSQGQAVKGNQLLPVSLVKRKTTLAPNTQTASPR
ALADSLMQLARQVSRLESGQ"
protein_bind 5820..5841
/label=CAP binding site
/bound_moiety="E. coli catabolite activator protein"
/note="CAP binding activates transcription in the presence
of cAMP."
promoter 5856..5886
/label=lac promoter
/note="promoter for the E. coli lac operon"
protein_bind 5894..5910
/label=lac operator
/bound_moiety="lac repressor encoded by lacI"
/note="The lac repressor binds to the lac operator to
inhibit transcription in E. coli. This inhibition can be
relieved by adding lactose or
isopropyl-beta-D-thiogalactopyranoside (IPTG)."
primer_bind 5918..5934
/label=M13 rev
/note="common sequencing primer, one of multiple similar
variants"
CDS 5930..6376
/codon_start=1
/gene="lacZ fragment"
/product="LacZ-alpha fragment of beta-galactosidase"
/label=lacZ-alpha
/translation="MTMITDSLAVVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEART
DRPSQQLRSLNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNV
TYPITVNPPFVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVGI"
primer_bind complement(5950..5966)
/label=M13 fwd
/note="common sequencing primer, one of multiple similar
variants"
ORIGIN
1 acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
61 gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
121 tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
181 tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
241 cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
301 aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
361 gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
421 ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
481 tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
541 ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
601 ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
661 atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
721 tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
781 aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
841 ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
901 atcctccaaa atcggatctg gaagttctgt tccaggggcc cctgggatcc gaaaacctgt
961 atttccaggg cggtagtatg gcacagcagg aagccagtcc gagccctggt gcagaagttg
1021 tgggccgcgc atttgttgaa cagtattatc atattctgca ccagagccct ggtctggtgc
1081 atcgttttta tcaggatagc agttttctga cccgtccgga tgttaccggt gccgttacca
1141 ccgtgaccac catgcaggca attaatgata aaattctgag tctgaagtac gaagattata
1201 ccgcagaaat tgaaaccgcc gatgcccagg aaagtcatga acgtggtgtt attgtgctgg
1261 ttaccggtcg cctgaccggt aatgataatg tgcgcaaaaa attttcacag agtttctttc
1321 tggcaccgca ggataaaggt tattttgtgc tgaatgatgt tttccgcttt ctggaagaaa
1381 aagaagtgac cgcacaggcc cgcagcgttc cgattaatgg caccacccgt gatgttcagg
1441 caccgattga accggaacgc gtggtggtta gccatgaacc ggaagtggaa ccggaaccgg
1501 ttgcaagcat tgaagaagaa gatctggata atgtggccga agtttatgat ccgagcgata
1561 aagatgaagg tgtggtggtt gatgtggaac cgattgaacc tccgacccag attagtcata
1621 atgaaattct gagcgtgccg cagggcgatg caccgaaaca tagttatgca agtattctga
1681 aacagatgaa aagcagcccg gccccgacca cccatgttgc acgcaataag ccgcgtccgg
1741 caccggtgaa tcagaaactg accgccccgc cggcagaacc ggcagcaaga cctgaagcca
1801 gtgcccatga aaatgttccg aatagcagcc atgtggatgt tgaagatgat ggtcatagca
1861 tctatgttcg caatctgccg tttgatagca ccccgaccca gctggaagaa gtgtttaaaa
1921 attttggtgc aatcaagcat gagggtattc aggttcgcag taataagcag cagggctttt
1981 gttttggttt tgtggaattt gaaaccagta gtggtaaaca gagtgcactg gaagcaagcc
2041 cggttaccat tggtgaccgt caggccgttg tggaagaaaa gaaaaccaat agtcgtggtg
2101 gtggcaataa tggcggtagc cgtggtcgtt attttagcgg ccgtggcagc tttcgcaatg
2161 aaagttttaa aggcggtcgt ggcggcggcg gccgtggtgg ttatggtcgt ggtggcggcg
2221 aattttctgg ccgtccgaaa agtagcaatc cgcgtaatgg tggcgaaggt tatcagcgcg
2281 tgccgcagaa tggtggtggt ggtcgcggcg gccgtggcga gggtggtcgt ggtggtgctc
2341 gcggtggcgg tagcagctaa ctcgagcggc cgcatcgtga ctgactgacg atctgcctcg
2401 cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag
2461 cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg
2521 gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg tataattctt
2581 gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg ataataatgg
2641 tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct atttgtttat
2701 ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga taaatgcttc
2761 aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc cttattccct
2821 tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg aaagtaaaag
2881 atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc aacagcggta
2941 agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact tttaaagttc
3001 tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc ggtcgccgca
3061 tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag catcttacgg
3121 atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat aacactgcgg
3181 ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt ttgcacaaca
3241 tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa gccataccaa
3301 acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc aaactattaa
3361 ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg gaggcggata
3421 aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt gctgataaat
3481 ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca gatggtaagc
3541 cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat gaacgaaata
3601 gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca gaccaagttt
3661 actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg atctaggtga
3721 agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg ttccactgag
3781 cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt ctgcgcgtaa
3841 tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg ccggatcaag
3901 agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata ccaaatactg
3961 tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca ccgcctacat
4021 acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag tcgtgtctta
4081 ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc tgaacggggg
4141 gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga tacctacagc
4201 gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg tatccggtaa
4261 gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac gcctggtatc
4321 tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg tgatgctcgt
4381 caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg ttcctggcct
4441 tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct gtggataacc
4501 gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc gagcgcagcg
4561 agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt acgcatctgt
4621 gcggtatttc acaccgcata aattccgaca ccatcgaatg gtgcaaaacc tttcgcggta
4681 tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa ccagtaacgt
4741 tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc gtggtgaacc
4801 aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg gcggagctga
4861 attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg ctgattggcg
4921 ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg attaaatctc
4981 gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc ggcgtcgaag
5041 cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg atcattaact
5101 atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat gttccggcgt
5161 tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc catgaagacg
5221 gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc gcgctgttag
5281 cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat aaatatctca
5341 ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc atgtccggtt
5401 ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg ctggttgcca
5461 acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg cgcgttggtg
5521 cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat atcccgccgt
5581 caaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac cgcttgctgc
5641 aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca ctggtgaaaa
5701 gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat
5761 taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt
5821 aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt
5881 atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat
5941 tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg gcgttaccca
6001 acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg aagaggcccg
6061 caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct ttgcctggtt
6121 tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg aggccgatac
6181 tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct acaccaacgt
6241 aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga cgggttgtta
6301 ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc gaattatttt
6361 tgatggcgtt ggaatt
//
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