pFRT/lacZeo

LOCUS       pFRT lacZeo               8106 bp ds-DNA     circular SYN 24-OCT-2017
DEFINITION  Flp recombination target site (FRT) vector, with an SV40 enhancer 
            and promoter, for generating a Flp-In(TM) host cell line. See also 
            pFRT/lacZeo2.
ACCESSION   .
VERSION     .
KEYWORDS    pFRT lacZeo
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8106)
  AUTHORS   Thermo Fisher (Invitrogen)
  TITLE     Direct Submission
  JOURNAL   Exported Saturday, November 30, 2019 from SnapGene Viewer 3.1.4
            http://www.snapgene.com
COMMENT     For stable transfection, it is recommended to linearize with ScaI or
            another enzyme that cuts outside the ATG-FRT-lacZ-BleoR cassette.
FEATURES             Location/Qualifiers
     source          1..8106
                     /organism="synthetic DNA construct"
                     /lab_host="Mammalian Cells"
                     /mol_type="other DNA"
     promoter        303..600
                     /note="SV40 promoter"
                     /note="SV40 enhancer and early promoter"
     rep_origin      470..604
                     /note="SV40 ori"
                     /note="SV40 origin of replication"
     CDS             609..611
                     /codon_start=1
                     /product="start codon"
                     /note="ATG"
                     /translation="M"
     protein_bind    614..661
                     /bound_moiety="FLP recombinase from the Saccharomyces 
                     cerevisiae 2u plasmid"
                     /note="FRT"
                     /note="FLP-mediated recombination occurs in the 8-bp core 
                     sequence TCTAGAAA (Turan and Bode, 2011)."
     CDS             678..3722
                     /codon_start=1
                     /gene="lacZ"
                     /product="beta-galactosidase"
                     /note="lacZ"
                     /translation="VVLQRRDWENPGVTQLNRLAAHPPFASWRNSEEARTDRPSQQLRS
                     LNGEWRFAWFPAPEAVPESWLECDLPEADTVVVPSNWQMHGYDAPIYTNVTYPITVNPP
                     FVPTENPTGCYSLTFNVDESWLQEGQTRIIFDGVNSAFHLWCNGRWVGYGQDSRLPSEF
                     DLSAFLRAGENRLAVMVLRWSDGSYLEDQDMWRMSGIFRDVSLLHKPTTQISDFHVATR
                     FNDDFSRAVLEAEVQMCGELRDYLRVTVSLWQGETQVASGTAPFGGEIIDERGGYADRV
                     TLRLNVENPKLWSAEIPNLYRAVVELHTADGTLIEAEACDVGFREVRIENGLLLLNGKP
                     LLIRGVNRHEHHPLHGQVMDEQTMVQDILLMKQNNFNAVRCSHYPNHPLWYTLCDRYGL
                     YVVDEANIETHGMVPMNRLTDDPRWLPAMSERVTRMVQRDRNHPSVIIWSLGNESGHGA
                     NHDALYRWIKSVDPSRPVQYEGGGADTTATDIICPMYARVDEDQPFPAVPKWSIKKWLS
                     LPGETRPLILCEYAHAMGNSLGGFAKYWQAFRQYPRLQGGFVWDWVDQSLIKYDENGNP
                     WSAYGGDFGDTPNDRQFCMNGLVFADRTPHPALTEAKHQQQFFQFRLSGQTIEVTSEYL
                     FRHSDNELLHWMVALDGKPLASGEVPLDVAPQGKQLIELPELPQPESAGQLWLTVRVVQ
                     PNATAWSEAGHISAWQQWRLAENLSVTLPAASHAIPHLTTSEMDFCIELGNKRWQFNRQ
                     SGFLSQMWIGDKKQLLTPLRDQFTRAPLDNDIGVSEATRIDPNAWVERWKAAGHYQAEA
                     ALLQCTADTLADAVLITTAHAWQHQGKTLFISRKTYRIDGSGQMAITVDVEVASDTPHP
                     ARIGLNCQLAQVAERVNWLGLGPQENYPDRLTAACFDRWDLPLSDMYTPYVFPSENGLR
                     CGTRELNYGPHQWRGDFQFNISRYSQQQLMETSHRHLLHAEEGTWLNIDGFHMGIGGDD
                     SWSPSVSAELQLSAGRYHYQLVWCQK"
     CDS             3804..4181
                     /codon_start=1
                     /gene="Sh ble from Streptoalloteichus hindustanus"
                     /product="antibiotic-binding protein"
                     /note="BleoR"
                     /note="confers resistance to bleomycin, phleomycin, and 
                     Zeocin(TM)"
                     /translation="MDAKLTSAVPVLTARDVAGAVEFWTDRLGFSRDFVEDDFAGVVRD
                     DVTLFISAVQDQVVPDNTLAWVWVRGLDELYAEWSEVVSTNFRDASGPAMTEIGEQPWG
                     REFALRDPAGNCVHFVAEEQD"
     intron          4568..4633
                     /note="small t intron"
                     /note="SV40 (simian virus 40) small t antigen intron"
     polyA_signal    5208..5342
                     /note="SV40 poly(A) signal"
                     /note="SV40 polyadenylation signal"
     promoter        6195..6299
                     /gene="bla"
                     /note="AmpR promoter"
     CDS             6300..7160
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /note="AmpR"
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRVDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPAAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      7331..7919
                     /direction=RIGHT
                     /note="ori"
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 tgatgcggta ttttctcctt acgcatctgt gcggtatttc acaccgcata tggtgcactc
       61 tcagtacaat ctgctctgat gccgcatagt taagccagta tacactccgc tatcgctacg
      121 tgactgggtc atggctgcgc cccgacaccc gccaacaccc gctgacgcgc cctgacgggc
      181 ttgtctgctc ccggcatccg cttacagaca agctgtgacc gtctccggga gctgcatgtg
      241 tcagaggttt tcaccgtcat caccgaaacg cgcgaggcag ctgtggaatg tgtgtcagtt
      301 agggtgtgga aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa
      361 ttagtcagca accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag
      421 catgcatctc aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct
      481 aactccgccc agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc
      541 agaggccgag gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg
      601 aggctaccat ggagaagtta ctattccgaa gttcctattc tctagaaagt ataggaactt
      661 caagcttggc actggccgtc gttttacaac gtcgtgactg ggaaaaccct ggcgttaccc
      721 aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc gaagaggccc
      781 gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc tttgcctggt
      841 ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct gaggccgata
      901 ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc tacaccaacg
      961 tgacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg acgggttgtt
     1021 actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg cgaattattt
     1081 ttgatggcgt taactcggcg tttcatctgt ggtgcaacgg gcgctgggtc ggttacggcc
     1141 aggacagtcg tttgccgtct gaatttgacc tgagcgcatt tttacgcgcc ggagaaaacc
     1201 gcctcgcggt gatggtgctg cgctggagtg acggcagtta tctggaagat caggatatgt
     1261 ggcggatgag cggcattttc cgtgacgtct cgttgctgca taaaccgact acacaaatca
     1321 gcgatttcca tgttgccact cgctttaatg atgatttcag ccgcgctgta ctggaggctg
     1381 aagttcagat gtgcggcgag ttgcgtgact acctacgggt aacagtttct ttatggcagg
     1441 gtgaaacgca ggtcgccagc ggcaccgcgc ctttcggcgg tgaaattatc gatgagcgtg
     1501 gtggttatgc cgatcgcgtc acactacgtc tgaacgtcga aaacccgaaa ctgtggagcg
     1561 ccgaaatccc gaatctctat cgtgcggtgg ttgaactgca caccgccgac ggcacgctga
     1621 ttgaagcaga agcctgcgat gtcggtttcc gcgaggtgcg gattgaaaat ggtctgctgc
     1681 tgctgaacgg caagccgttg ctgattcgag gcgttaaccg tcacgagcat catcctctgc
     1741 atggtcaggt catggatgag cagacgatgg tgcaggatat cctgctgatg aagcagaaca
     1801 actttaacgc cgtgcgctgt tcgcattatc cgaaccatcc gctgtggtac acgctgtgcg
     1861 accgctacgg cctgtatgtg gtggatgaag ccaatattga aacccacggc atggtgccaa
     1921 tgaatcgtct gaccgatgat ccgcgctggc taccggcgat gagcgaacgc gtaacgcgaa
     1981 tggtgcagcg cgatcgtaat cacccgagtg tgatcatctg gtcgctgggg aatgaatcag
     2041 gccacggcgc taatcacgac gcgctgtatc gctggatcaa atctgtcgat ccttcccgcc
     2101 cggtgcagta tgaaggcggc ggagccgaca ccacggccac cgatattatt tgcccgatgt
     2161 acgcgcgcgt ggatgaagac cagcccttcc cggctgtgcc gaaatggtcc atcaaaaaat
     2221 ggctttcgct acctggagag acgcgcccgc tgatcctttg cgaatacgcc cacgcgatgg
     2281 gtaacagtct tggcggtttc gctaaatact ggcaggcgtt tcgtcagtat ccccgtttac
     2341 agggcggctt cgtctgggac tgggtggatc agtcgctgat taaatatgat gaaaacggca
     2401 acccgtggtc ggcttacggc ggtgattttg gcgatacgcc gaacgatcgc cagttctgta
     2461 tgaacggtct ggtctttgcc gaccgcacgc cgcatccagc gctgacggaa gcaaaacacc
     2521 agcagcagtt tttccagttc cgtttatccg ggcaaaccat cgaagtgacc agcgaatacc
     2581 tgttccgtca tagcgataac gagctcctgc actggatggt ggcgctggat ggtaagccgc
     2641 tggcaagcgg tgaagtgcct ctggatgtcg ctccacaagg taaacagttg attgaactgc
     2701 ctgaactacc gcagccggag agcgccgggc aactctggct cacagtacgc gtagtgcaac
     2761 cgaacgcgac cgcatggtca gaagccggcc acatcagcgc ctggcagcag tggcgtctgg
     2821 cggaaaacct cagtgtgacg ctccccgccg cgtcccacgc catcccgcat ctgaccacca
     2881 gcgaaatgga tttttgcatc gagctgggta ataagcgttg gcaatttaac cgccagtcag
     2941 gctttctttc acagatgtgg attggcgata aaaaacaact gctgacgccg ctgcgcgatc
     3001 agttcacccg tgcaccgctg gataacgaca ttggcgtaag tgaagcgacc cgcattgacc
     3061 ctaacgcctg ggtcgaacgc tggaaggcgg cgggccatta ccaggccgaa gcagcgttgt
     3121 tgcagtgcac ggcagataca cttgctgacg cggtgctgat tacgaccgct cacgcgtggc
     3181 agcatcaggg gaaaacctta tttatcagcc ggaaaaccta ccggattgat ggtagtggtc
     3241 aaatggcgat taccgttgat gttgaagtgg cgagcgatac accgcatccg gcgcggattg
     3301 gcctgaactg ccagctggcg caggtagcag agcgggtaaa ctggctcgga ttagggccgc
     3361 aagaaaacta tcccgaccgc cttactgccg cctgttttga ccgctgggat ctgccattgt
     3421 cagacatgta taccccgtac gtcttcccga gcgaaaacgg tctgcgctgc gggacgcgcg
     3481 aattgaatta tggcccacac cagtggcgcg gcgacttcca gttcaacatc agccgctaca
     3541 gtcaacagca actgatggaa accagccatc gccatctgct gcacgcggaa gaaggcacat
     3601 ggctgaatat cgacggtttc catatgggga ttggtggaga cgactcctgg agcccgtcag
     3661 tatcggcgga attacagctg agcgccggtc gctaccatta ccagttggtc tggtgtcaaa
     3721 aagcgctttc gaaagatccc aacgaaaagc gtgaccacat ggtccttctt gagtttgtaa
     3781 ctgctgctgg gattacacat ggcatggatg ccaagttgac cagtgccgtt ccggtgctca
     3841 ccgcgcgcga cgtcgccgga gcggtcgagt tctggaccga ccggctcggg ttctcccggg
     3901 acttcgtgga ggacgacttc gccggtgtgg tccgggacga cgtgaccctg ttcatcagcg
     3961 cggtccagga ccaggtggtg ccggacaaca ccctggcctg ggtgtgggtg cgcggcctgg
     4021 acgagctgta cgccgagtgg tcggaggtcg tgtccacgaa cttccgggac gcctccgggc
     4081 cggccatgac cgagatcggc gagcagccgt gggggcggga gttcgccctg cgcgacccgg
     4141 ccggcaactg cgtgcacttc gtggccgagg agcaggactg acacccgagc gaaaacggtc
     4201 tgcgctgcgg gacgcgcgaa ttgaattatg gcccacacca gtggcgcggc gacttccagt
     4261 tcaacatcag ccgctacagt caacagcaac tgatggaaac cagccatcgc catctgctgc
     4321 acgcggaaga aggcacatgg ctgaatatcg acggtttcca tatggggatt ggtggcgacg
     4381 actcctggag cccgtcagta tcggcggaat tccagctgag cgccggtcgc taccattacc
     4441 agttggtctg gtgtcaaaaa taataataac cgggcagggg ggatctgcat ggatctttgt
     4501 gaaggaacct tacttctgtg gtgtgacata attggacaaa ctacctacag agatttaaag
     4561 ctctaaggta aatataaaat ttttaagtgt ataatgtgtt aaactactga ttctaattgt
     4621 ttgtgtattt tagattccaa cctatggaac tgatgaatgg gagcagtggt ggaatgcctt
     4681 taatgaggaa aacctgtttt gctcagaaga aatgccatct agtgatgatg aggctactgc
     4741 tgactctcaa cattctactc ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt
     4801 tccttcagaa ttgctaagtt ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg
     4861 ctttgctatt tacaccacaa aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa
     4921 atattctgta acctttataa gtaggcataa cagttataat cataacatac tgttttttct
     4981 tactccacac aggcatagag tgtctgctat taataactat gctcaaaaat tgtgtacctt
     5041 tagcttttta atttgtaaag gggttaataa ggaatatttg atgtatagtg ccttgactag
     5101 agatcataat cagccatacc acatttgtag aggttttact tgctttaaaa aacctcccac
     5161 acctccccct gaacctgaaa cataaaatga atgcaattgt tgttgttaac ttgtttattg
     5221 cagcttataa tggttacaaa taaagcaata gcatcacaaa tttcacaaat aaagcatttt
     5281 tttcactgca ttctagttgt ggtttgtcca aactcatcaa tgtatcttat catgtctgga
     5341 tccccaggaa gctcctctgt gtcctcataa accctaacct cctctacttg agaggacatt
     5401 ccaatcatag gctgcccatc caccctctgt gtcctcctgt taattaggtc acttaacaaa
     5461 aaggaaattg ggtaggggtt tttcacagac cgctttctaa gggtaatttt aaaatatctg
     5521 ggaagtccct tccactgctg tgttccagaa gtgttggtaa acagcccaca aatgtcaaca
     5581 gcagaaacat acaagctgtc agctttgcac aagggcccaa caccctgctc atcaagaagc
     5641 actgtggttg ctgtgttagt aatgtgcaaa acaggaggca cattttcccc acctgtgtag
     5701 gttccaaaat atctagtgtt ttcattttta cttggatcag gaacccagca ctccactgga
     5761 taagcattat ccttatccaa aacagccttg tggtcagtgt tcatctgctg actgtcaact
     5821 gtagcatttt ttggggttac agtttgagca ggatatttgg tcctgtagtt tgctaacaca
     5881 ccctgcagct ccaaaggttc cccaccaaca gcaaaaaaat gaaaatttga cccttgaatg
     5941 ggttttccag caccattttc atgagttttt tgtgtccctg aatgcaagtt taacatagca
     6001 gttaccccaa taacctcagt tttaacagta acagcttccc acatcaaaat atttccacag
     6061 gttaagtcct catttaaatt aggcaaagga attcttgaag acgaaagggc ctcgtgatac
     6121 gcctattttt ataggttaat gtcatgataa taatggtttc ttagacgtca ggtggcactt
     6181 ttcggggaaa tgtgcgcgga acccctattt gtttattttt ctaaatacat tcaaatatgt
     6241 atccgctcat gagacaataa ccctgataaa tgcttcaata atattgaaaa aggaagagta
     6301 tgagtattca acatttccgt gtcgccctta ttcccttttt tgcggcattt tgccttcctg
     6361 tttttgctca cccagaaacg ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac
     6421 gagtgggtta catcgaactg gatctcaaca gcggtaagat ccttgagagt tttcgccccg
     6481 aagaacgttt tccaatgatg agcactttta aagttctgct atgtggcgcg gtattatccc
     6541 gtgttgacgc cgggcaagag caactcggtc gccgcataca ctattctcag aatgacttgg
     6601 ttgagtactc accagtcaca gaaaagcatc ttacggatgg catgacagta agagaattat
     6661 gcagtgctgc cataaccatg agtgataaca ctgcggccaa cttacttctg acaacgatcg
     6721 gaggaccgaa ggagctaacc gcttttttgc acaacatggg ggatcatgta actcgccttg
     6781 atcgttggga accggagctg aatgaagcca taccaaacga cgagcgtgac accacgatgc
     6841 ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg cgaactactt actctagctt
     6901 cccggcaaca attaatagac tggatggagg cggataaagt tgcaggacca cttctgcgct
     6961 cggcccttcc ggctggctgg tttattgctg ataaatctgg agccggtgag cgtgggtctc
     7021 gcggtatcat tgcagcactg gggccagatg gtaagccctc ccgtatcgta gttatctaca
     7081 cgacggggag tcaggcaact atggatgaac gaaatagaca gatcgctgag ataggtgcct
     7141 cactgattaa gcattggtaa ctgtcagacc aagtttactc atatatactt tagattgatt
     7201 taaaacttca tttttaattt aaaaggatct aggtgaagat cctttttgat aatctcatga
     7261 ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc agaccccgta gaaaagatca
     7321 aaggatcttc ttgagatcct ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac
     7381 caccgctacc agcggtggtt tgtttgccgg atcaagagct accaactctt tttccgaagg
     7441 taactggctt cagcagagcg cagataccaa atactgtcct tctagtgtag ccgtagttag
     7501 gccaccactt caagaactct gtagcaccgc ctacatacct cgctctgcta atcctgttac
     7561 cagtggctgc tgccagtggc gataagtcgt gtcttaccgg gttggactca agacgatagt
     7621 taccggataa ggcgcagcgg tcgggctgaa cggggggttc gtgcacacag cccagcttgg
     7681 agcgaacgac ctacaccgaa ctgagatacc tacagcgtga gctatgagaa agcgccacgc
     7741 ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg cagggtcgga acaggagagc
     7801 gcacgaggga gcttccaggg ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc
     7861 acctctgact tgagcgtcga tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa
     7921 acgccagcaa cgcggccttt ttacggttcc tggccttttg ctggcctttt gctcacatgt
     7981 tctttcctgc gttatcccct gattctgtgg ataaccgtat taccgccttt gagtgagctg
     8041 ataccgctcg ccgcagccga acgaccgagc gcagcgagtc agtgagcgag gaagcggaag
     8101 agcgcc
//