pCDH-MTS-GFP-MECR

LOCUS       pCDH-MTS-GFP-MECR            8391 bp DNA     circular SYN 02-JUL-2026
DEFINITION  contig1 circular.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 8391)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Aug 24, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..8391
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     promoter        6..232
                     /label=RSV promoter
                     /note="Rous sarcoma virus enhancer/promoter"
     LTR             233..413
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from HIV-1"
     misc_feature    460..585
                     /label=HIV-1 Psi
                     /note="packaging signal of human immunodeficiency virus 
                     type 1"
     misc_feature    1078..1311
                     /label=RRE
                     /note="The Rev response element (RRE) of HIV-1 allows for 
                     Rev-dependent mRNA export from the nucleus to the 
                     cytoplasm."
     CDS             1496..1540
                     /codon_start=1
                     /product="antigenic peptide corresponding to amino acids 
                     655 to 669 of the HIV envelope protein gp41 (Lutje Hulsik 
                     et al., 2013)"
                     /label=gp41 peptide
                     /note="recognized by the 2H10 single-chain llama nanobody"
                     /translation="KNEQELLELDKWASL"
     misc_feature    1807..1923
                     /label=cPPT/CTS
                     /note="central polypurine tract and central termination 
                     sequence of HIV-1"
     enhancer        1946..2249
                     /label=CMV enhancer
                     /note="human cytomegalovirus immediate early enhancer"
     promoter        2250..2453
                     /label=CMV promoter
                     /note="human cytomegalovirus (CMV) immediate early 
                     promoter"
     misc_feature    2507..2599
                     /label=MTS
     CDS             2600..3313
                     /codon_start=1
                     /product="enhanced GFP with monomerizing A206K mutation 
                     (Zacharias et al., 2002)"
                     /label=mEGFP
                     /note="mammalian codon-optimized"
                     /translation="VSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTLK
                     FICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDDG
                     NYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIKV
                     NFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSKLSKDPNEKRDHMVLLE
                     FVTAAGITLGMDELYK"
     misc_feature    3344..4465
                     /label=MECR
     misc_feature    4472..5059
                     /label=WPRE
                     /note="woodchuck hepatitis virus posttranscriptional 
                     regulatory element"
     CDS             complement(4943..4954)
                     /codon_start=1
                     /product="Factor Xa recognition and cleavage site"
                     /label=Factor Xa site
                     /translation="IEGR"
     LTR             5132..5365
                     /label=3' LTR (Delta-U3)
                     /note="self-inactivating 3' long terminal repeat (LTR) from
                     HIV-1"
     polyA_signal    5437..5558
                     /label=SV40 poly(A) signal
                     /note="SV40 polyadenylation signal"
     rep_origin      5598..5733
                     /label=SV40 ori
                     /note="SV40 origin of replication"
     primer_bind     complement(5766..5782)
                     /label=M13 rev
                     /note="common sequencing primer, one of multiple similar 
                     variants"
     protein_bind    5790..5806
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(5814..5844)
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    5859..5880
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     rep_origin      complement(6168..6756)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     CDS             complement(6927..7787)
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     promoter        complement(7788..7892)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     8366..8382
                     /label=M13 fwd
                     /note="common sequencing primer, one of multiple similar 
                     variants"
ORIGIN
        1 acgcgtgtag tcttatgcaa tactcttgta gtcttgcaac atggtaacga tgagttagca
       61 acatgcctta caaggagaga aaaagcaccg tgcatgccga ttggtggaag taaggtggta
      121 cgatcgtgcc ttattaggaa ggcaacagac gggtctgaca tggattggac gaaccactga
      181 attgccgcat tgcagagata ttgtatttaa gtgcctagct cgatacataa acgggtctct
      241 ctggttagac cagatctgag cctgggagct ctctggctaa ctagggaacc cactgcttaa
      301 gcctcaataa agcttgcctt gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc
      361 tggtaactag agatccctca gaccctttta gtcagtgtgg aaaatctcta gcagtggcgc
      421 ccgaacaggg acttgaaagc gaaagggaaa ccagaggagc tctctcgacg caggactcgg
      481 cttgctgaag cgcgcacggc aagaggcgag gggcggcgac tggtgagtac gccaaaaatt
      541 ttgactagcg gaggctagaa ggagagagat gggtgcgaga gcgtcagtat taagcggggg
      601 agaattagat cgcgatggga aaaaattcgg ttaaggccag ggggaaagaa aaaatataaa
      661 ttaaaacata tagtatgggc aagcagggag ctagaacgat tcgcagttaa tcctggcctg
      721 ttagaaacat cagaaggctg tagacaaata ctgggacagc tacaaccatc ccttcagaca
      781 ggatcagaag aacttagatc attatataat acagtagcaa ccctctattg tgtgcatcaa
      841 aggatagaga taaaagacac caaggaagct ttagacaaga tagaggaaga gcaaaacaaa
      901 agtaagacca ccgcacagca agcggccact gatcttcaga cctggaggag gagatatgag
      961 ggacaattgg agaagtgaat tatataaata taaagtagta aaaattgaac cattaggagt
     1021 agcacccacc aaggcaaaga gaagagtggt gcagagagaa aaaagagcag tgggaatagg
     1081 agctttgttc cttgggttct tgggagcagc aggaagcact atgggcgcag cgtcaatgac
     1141 gctgacggta caggccagac aattattgtc tggtatagtg cagcagcaga acaatttgct
     1201 gagggctatt gaggcgcaac agcatctgtt gcaactcaca gtctggggca tcaagcagct
     1261 ccaggcaaga atcctggctg tggaaagata cctaaaggat caacagctcc tggggatttg
     1321 gggttgctct ggaaaactca tttgcaccac tgctgtgcct tggaatgcta gttggagtaa
     1381 taaatctctg gaacagattt ggaatcacac gacctggatg gagtgggaca gagaaattaa
     1441 caattacaca agcttaatac actccttaat tgaagaatcg caaaaccagc aagaaaagaa
     1501 tgaacaagaa ttattggaat tagataaatg ggcaagtttg tggaattggt ttaacataac
     1561 aaattggctg tggtatataa aattattcat aatgatagta ggaggcttgg taggtttaag
     1621 aatagttttt gctgtacttt ctatagtgaa tagagttagg cagggatatt caccattatc
     1681 gtttcagacc cacctcccaa ccccgagggg acccgacagg cccgaaggaa tagaagaaga
     1741 aggtggagag agagacagag acagatccat tcgattagtg aacggatctc gacggtatcg
     1801 gttaactttt aaaagaaaag gggggattgg ggggtacagt gcaggggaaa gaatagtaga
     1861 cataatagca acagacatac aaactaaaga attacaaaaa caaattacaa aattcaaaat
     1921 tttatcgata ctagttggag ttccgcgtta cataacttac ggtaaatggc ccgcctggct
     1981 gaccgcccaa cgacccccgc ccattgacgt caataatgac gtatgttccc atagtaacgc
     2041 caatagggac tttccattga cgtcaatggg tggagtattt acggtaaact gcccacttgg
     2101 cagtacatca agtgtatcat atgccaagta cgccccctat tgacgtcaat gacggtaaat
     2161 ggcccgcctg gcattatgcc cagtacatga ccttatggga ctttcctact tggcagtaca
     2221 tctacgtatt agtcatcgct attaccatgg tgatgcggtt ttggcagtac atcaatgggc
     2281 gtggatagcg gtttgactca cggggatttc caagtctcca ccccattgac gtcaatggga
     2341 gtttgttttg gcaccaaaat caacgggact ttccaaaatg tcgtaacaac tccgccccat
     2401 tgacgcaaat gggcggtagg cgtgtacggt gggaggtcta tataagcaga gctggtttag
     2461 tgaaccgtca gattctagac cgctagcgct accggtcgcc accatgctgt ttaatctgag
     2521 gatcctgtta aacaatgcag cttttagaaa tggtcacaac ttcatggttc gaaattttcg
     2581 gtgtggacaa ccactacaag tgagcaaggg cgaggagctg ttcaccgggg tggtgcccat
     2641 cctggtcgag ctggacggcg acgtaaacgg ccacaagttc agcgtgtccg gcgagggcga
     2701 gggcgatgcc acctacggca agctgaccct gaagttcatc tgcaccaccg gcaagctgcc
     2761 cgtgccctgg cccaccctcg tgaccaccct gacctacggc gtgcagtgct tcagccgcta
     2821 ccccgaccac atgaagcagc acgacttctt caagtccgcc atgcccgaag gctacgtcca
     2881 ggagcgcacc atcttcttca aggacgacgg caactacaag acccgcgccg aggtgaagtt
     2941 cgagggcgac accctggtga accgcatcga gctgaagggc atcgacttca aggaggacgg
     3001 caacatcctg gggcacaagc tggagtacaa ctacaacagc cacaacgtct atatcatggc
     3061 cgacaagcag aagaacggca tcaaggtgaa cttcaagatc cgccacaaca tcgaggacgg
     3121 cagcgtgcag ctcgccgacc actaccagca gaacaccccc atcggcgacg gccccgtgct
     3181 gctgcccgac aaccactacc tgagcaccca gtccaagctg agcaaagacc ccaacgagaa
     3241 gcgcgatcac atggtcctgc tggagttcgt gaccgccgcc gggatcactc tcggcatgga
     3301 cgagctgtac aagtccggac tcagatctcg agctcaagct tccgtgtggg tctgcagtac
     3361 cctgtggcgg gtgcgaaccc ccgcccggca gtggcggggg ctgctcccag cttctggctg
     3421 tcacggacct gccgcctcct cctactccgc atccgccgag cctgcccggg tccgggcgct
     3481 tgtctatggg caccacgggg atccagccaa ggtcgtcgaa ctcaagaacc tggagctagc
     3541 tgctgtgaga ggatcagatg tccgtgtgaa gatgctggcg gcccctatca atccatctga
     3601 cataaatatg atccaaggaa actacggact ccttcctgaa ctgcctgctg ttggagggaa
     3661 cgaaggtgtt gcacaggtgg tagcggtggg cagcaatgtg accgggctga agccaggaga
     3721 ctgggtgatt ccagcaaatg ctggtttagg aacctggcgg accgaggctg tgttcagcga
     3781 ggaagcactg atccaagttc cgagtgacat ccctcttcag agcgctgcca ccctgggtgt
     3841 caatccctgc acagcctaca ggatgttgat ggacttcgag caactgcagc caggggattc
     3901 tgtcatccag aatgcatcca acagcggagt ggggcaagcg gtcatccaga tcgccgcagc
     3961 cctgggccta agaaccatca atgtggtccg agacagacct gatatccaga agctgagtga
     4021 cagactgaag agtctggggg ctgagcatgt catcacagaa gaggagctaa gaaggcccga
     4081 aatgaaaaac ttctttaagg acatgcccca gccacggctt gctctcaact gtgttggtgg
     4141 gaaaagctcc acagagctgc tgcggcagtt agcgcgtgga ggaaccatgg taacctatgg
     4201 ggggatggcc aagcagcccg tcgtagcctc tgtgagcctg ctcattttta aggatctcaa
     4261 acttcgaggc ttttggttgt cccagtggaa gaaggatcac agtccagacc agttcaagga
     4321 gctgatcctc acactgtgcg atctcatccg ccgaggccag ctcacagccc ctgcctgctc
     4381 ccaggtcccg ctgcaggact accagtctgc cttggaagcc tccatgaagc ccttcatatc
     4441 ttcaaagcag attctcacca tgtgaggatc caatcaacct ctggattaca aaatttgtga
     4501 aagattgact ggtattctta actatgttgc tccttttacg ctatgtggat acgctgcttt
     4561 aatgcctttg tatcatgcta ttgcttcccg tatggctttc attttctcct ccttgtataa
     4621 atcctggttg ctgtctcttt atgaggagtt gtggcccgtt gtcaggcaac gtggcgtggt
     4681 gtgcactgtg tttgctgacg caacccccac tggttggggc attgccacca cctgtcagct
     4741 cctttccggg actttcgctt tccccctccc tattgccacg gcggaactca tcgccgcctg
     4801 ccttgcccgc tgctggacag gggctcggct gttgggcact gacaattccg tggtgttgtc
     4861 ggggaaatca tcgtcctttc cttggctgct cgcctgtgtt gccacctgga ttctgcgcgg
     4921 gacgtccttc tgctacgtcc cttcggccct caatccagcg gaccttcctt cccgcggcct
     4981 gctgccggct ctgcggcctc ttccgcgtct ccgccttcgc cctcagacga gtcggatctc
     5041 cctttggccg cctccccgcc ggtaccttta agaccaatga cttacaaggc agctgtagat
     5101 cttagccact ttttaaaaga aaagggggga ctggaagggc taattcactc ccaacgaaga
     5161 taagatctgc tttttgcttg tactgggtct ctctggttag accagatctg agcctgggag
     5221 ctctctggct aactagggaa cccactgctt aagcctcaat aaagcttgcc ttgagtgctt
     5281 caagtagtgt gtgcccgtct gttgtgtgac tctggtaact agagatccct cagacccttt
     5341 tagtcagtgt ggaaaatctc tagcagtagt agttcatgtc atcttattat tcagtattta
     5401 taacttgcaa agaaatgaat atcagagagt gagaggaact tgtttattgc agcttataat
     5461 ggttacaaat aaagcaatag catcacaaat ttcacaaata aagcattttt ttcactgcat
     5521 tctagttgtg gtttgtccaa actcatcaat gtatcttatc atgtctggct ctagctatcc
     5581 cgcccctaac tccgcccatc ccgcccctaa ctccgcccag ttccgcccat tctccgcccc
     5641 atggctgact aatttttttt atttatgcag aggccgaggc cgcctcggcc tctgagctat
     5701 tccagaagta gtgaggaggc ttttttggag gcctagactt ttgcagagac caaattcgta
     5761 atcatgtcat agctgtttcc tgtgtgaaat tgttatccgc tcacaattcc acacaacata
     5821 cgagccggaa gcataaagtg taaagcctgg ggtgcctaat gagtgagcta actcacatta
     5881 attgcgttgc gctcactgcc cgctttccag tcgggaaacc tgtcgtgcca gctgcattaa
     5941 tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     6001 ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     6061 gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     6121 ggccagcaaa aggccaggaa ccttaaaaag gccgcgttgc tggcgttttt ccataggctc
     6181 cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     6241 ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     6301 accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     6361 catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     6421 gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     6481 tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     6541 agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     6601 actagaagaa cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     6661 gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     6721 aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     6781 gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     6841 aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     6901 atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     6961 gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     7021 atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     7081 ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     7141 cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     7201 agttcgccag ttaatagttt gcgcaacgtt gttgccattg ctacaggcat cgtggtgtca
     7261 cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     7321 tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     7381 agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     7441 gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     7501 gaatagtgta tgcggcgacc gagttgctct tgcccggcgt caatacggga taataccgcg
     7561 ccacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     7621 tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     7681 tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     7741 gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     7801 caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     7861 atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     7921 gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     7981 tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     8041 acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     8101 gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     8161 agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     8221 aggcgccatt cgccattcag gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct
     8281 tcgctattac gccagctggc gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg
     8341 ccagggtttt cccagtcacg acgttgtaaa acgacggcca gtgccaagct g
//