pQL127

LOCUS       pQL127                7129 bp DNA     circular SYN 07-APR-2026
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  recombinant plasmid
REFERENCE   1  (bases 1 to 7129)
  AUTHORS   Westlake University
  TITLE     Direct Submission
  JOURNAL   Exported Sep 2, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7129
                     /mol_type="other DNA"
                     /organism="recombinant plasmid"
     primer_bind     186..207
                     /label=olyl419
     misc_feature    235..818
                     /label=P_CMV
                     /label=nonstandard type: promoter_const
     misc_feature    235..614
                     /label=P_CMV_enhancer
                     /label=nonstandard type: promoter_element
     misc_feature    615..818
                     /label=P_CMV_IE
                     /label=nonstandard type: promoter_element
     primer_bind     769..789
                     /label=oMHS-CMV-F
     misc_feature    829
                     /label=putative transcriptional start
     promoter        863..882
                     /label=T7 promoter
     primer_bind     complement(871..894)
                     /label=olyl418
     primer_bind     881..921
                     /label=QL317 NFAT3-F
     primer_bind     881..882
                     /label=T7
     misc_feature    901..2691
                     /label=NFAT3
     primer_bind     complement(2671..2711)
                     /label=QL318 NFAT3-R
     terminator      2726..2953
                     /label=bGH polyA
     rep_origin      2999..3427
                     /label=f1 bacteriophage origin of replication
                     /label=f1 bacteriophage origin of replication; arrow _f1 
                     ori
     misc_feature    3441..3770
                     /label=P_SV40
                     /label=nonstandard type: promoter_const
     rep_origin      3621..3756
                     /label=SV40 ORI
     misc_feature    3673..3722
                     /label=P_SV40 TSS-region
     CDS             3837..4631
                     /label=Translation 2136-2930
     misc_feature    3837..4628
                     /label=Kan/neoR
                     /label=nonstandard type: resistance
     primer_bind     complement(4971..4991)
                     /label=M13-rev
     misc_binding    complement(4997..5019)
                     /label=LacO
     promoter        complement(5023..5053)
                     /label=lac promoter
     rep_origin      complement(5377..5962)
                     /direction=LEFT
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin o
                     /label=high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     _ori
     CDS             complement(6133..6993)
                     /label=Translation 5292-4432
     misc_feature    complement(6136..6993)
                     /label=AmpR
                     /label=nonstandard type: resistance
     promoter        complement(6994..7098)
                     /label=AmpR promoter
ORIGIN
        1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
       61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
      121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
      181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
      241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
      301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
      361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
      421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
      481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
      541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
      601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
      661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
      721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
      781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
      841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
      901 atgtttattg ggacagcaga tgatcgatat ttacgacctc atgcatttta ccaggtgcat
      961 cgaatcactg ggaagacagt cgctactgca agccaagaga taataattgc cagtacaaaa
     1021 gttctggaaa ttccacttct tcctgaaaat aatatgtcag ccagtattga ttgtgcaggt
     1081 attttgaaac tccgcaattc agatatagaa cttcgaaaag gagaaactga tattggcaga
     1141 aagaatacta gagtacgact tgtgtttcgt gtacacatcc cacagcccag tggaaaagtc
     1201 ctttctctgc agatagcctc tatacccgtt gagtgctccc agcggtctgc tcaagaactt
     1261 cctcatattg agaagtacag tatcaacagt tgttctgtaa atggaggtca tgaaatggtt
     1321 gtgactggat ctaattttct tccagaatcc aaaatcattt ttcttgaaaa aggacaagat
     1381 ggacgacctc agtgggaggt agaagggaag ataatcaggg aaaaatgtca aggggctcac
     1441 attgtccttg aagttcctcc atatcataac ccagcagtta cagctgcagt gcaggtgcac
     1501 ttttatcttt gcaatggcaa gaggaaaaaa agccagtctc aacgttttac ttatacacca
     1561 gttttgatga agcaagaaca cagagaagag attgatttgt cttcagttcc atctttgcct
     1621 gtgcctcatc ctgctcagac ccagaggcct tcctctgatt cagggtgttc acatgacagt
     1681 gtactgtcag gacagagaag tttgatttgc tccatcccac aaacatatgc atccatggtg
     1741 acctcatccc atctgccaca gttgcagtgt agagatgaga gtgttagtaa agaacagcat
     1801 atgattcctt ctccaattgt acaccagcct tttcaagtca caccaacacc tcctgtgggg
     1861 tcttcctatc agcctatgca aactaatgtt gtgtacaatg gaccaacttg tcttcctatt
     1921 aatgctgcct ctagtcaaga atttgattca gttttgtttc agcaggatgc aactctttct
     1981 ggtttagtga atcttggctg tcaaccactg tcatccatac catttcattc ttcaaattca
     2041 ggctcaacag gacatctctt agcccataca cctcattctg tgcataccct gcctcatctg
     2101 caatcaatgg gatatcattg ttcaaataca ggacaaagat ctctttcttc tccagtggct
     2161 gaccagatta caggtcagcc ttcgtctcag ttacaaccta ttacatatgg tccttcacat
     2221 tcagggtctg ctacaacagc ttccccagca gcttctcatc ccttggctag ttcaccgctt
     2281 tctgggccac catctcctca gcttcagcct atgccttacc aatctcctag ctcaggaact
     2341 gcctcatcac cgtctccagc caccagaatg cattctggac agcactcaac tcaagcacaa
     2401 agtacgggcc aggggggtct ttctgcacct tcatccttaa tatgtcacag tttgtgtgat
     2461 ccagcgtcat ttccacctga tggggcaact gtgagcatta aacctgaacc agaagatcga
     2521 gagcctaact ttgcaaccat tggtctgcag gacatcactt tagatgatgt gaacgagata
     2581 attgggagag acatgtccca gatttctgtt tcccaaggag caggggtgag caggcaggct
     2641 cccctcccga gtcctgagtc cctggattta ggaagatctg atgggctcta atctagaggg
     2701 cccgtttaaa cccgctgatc agcctcgact gtgccttcta gttgccagcc atctgttgtt
     2761 tgcccctccc ccgtgccttc cttgaccctg gaaggtgcca ctcccactgt cctttcctaa
     2821 taaaatgagg aaattgcatc gcattgtctg agtaggtgtc attctattct ggggggtggg
     2881 gtggggcagg acagcaaggg ggaggattgg gaagacaata gcaggcatgc tggggatgcg
     2941 gtgggctcta tggcttctga ggcggaaaga accagctggg gctctagggg gtatccccac
     3001 gcgccctgta gcggcgcatt aagcgcggcg ggtgtggtgg ttacgcgcag cgtgaccgct
     3061 acacttgcca gcgccctagc gcccgctcct ttcgctttct tcccttcctt tctcgccacg
     3121 ttcgccggct ttccccgtca agctctaaat cgggggctcc ctttagggtt ccgatttagt
     3181 gctttacggc acctcgaccc caaaaaactt gattagggtg atggttcacg tagtgggcca
     3241 tcgccctgat agacggtttt tcgccctttg acgttggagt ccacgttctt taatagtgga
     3301 ctcttgttcc aaactggaac aacactcaac cctatctcgg tctattcttt tgatttataa
     3361 gggattttgc cgatttcggc ctattggtta aaaaatgagc tgatttaaca aaaatttaac
     3421 gcgaattaat tctgtggaat gtgtgtcagt tagggtgtgg aaagtcccca ggctccccag
     3481 caggcagaag tatgcaaagc atgcatctca attagtcagc aaccaggtgt ggaaagtccc
     3541 caggctcccc agcaggcaga agtatgcaaa gcatgcatct caattagtca gcaaccatag
     3601 tcccgcccct aactccgccc atcccgcccc taactccgcc cagttccgcc cattctccgc
     3661 cccatggctg actaattttt tttatttatg cagaggccga ggccgcctct gcctctgagc
     3721 tattccagaa gtagtgagga ggcttttttg gaggcctagg cttttgcaaa aagctcccgg
     3781 gagcttgtat atccattttc ggatctgatc aagagacagg atgaggatcg tttcgcatga
     3841 ttgaacaaga tggattgcac gcaggttctc cggccgcttg ggtggagagg ctattcggct
     3901 atgactgggc acaacagaca atcggctgct ctgatgccgc cgtgttccgg ctgtcagcgc
     3961 aggggcgccc ggttcttttt gtcaagaccg acctgtccgg tgccctgaat gaactgcagg
     4021 acgaggcagc gcggctatcg tggctggcca cgacgggcgt tccttgcgca gctgtgctcg
     4081 acgttgtcac tgaagcggga agggactggc tgctattggg cgaagtgccg gggcaggatc
     4141 tcctgtcatc tcaccttgct cctgccgaga aagtatccat catggctgat gcaatgcggc
     4201 ggctgcatac gcttgatccg gctacctgcc cattcgacca ccaagcgaaa catcgcatcg
     4261 agcgagcacg tactcggatg gaagccggtc ttgtcgatca ggatgatctg gacgaagagc
     4321 atcaggggct cgcgccagcc gaactgttcg ccaggctcaa ggcgcgcatg cccgacggcg
     4381 aggatctcgt cgtgacccat ggcgatgcct gcttgccgaa tatcatggtg gaaaatggcc
     4441 gcttttctgg attcatcgac tgtggccggc tgggtgtggc ggaccgctat caggacatag
     4501 cgttggctac ccgtgatatt gctgaagagc ttggcggcga atgggctgac cgcttcctcg
     4561 tgctttacgg tatcgccgct cccgattcgc agcgcatcgc cttctatcgc cttcttgacg
     4621 agttcttctg agcgggactc tggggttcga aatgaccgac caagcgacgc ccaacctgcc
     4681 atcacgagat ttcgattcca ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt
     4741 ccgggacgcc ggctggatga tcctccagcg cggggatctc atgctggagt tcttcgccca
     4801 ccccaacttg tttattgcag cttataatgg ttacaaataa agcaatagca tcacaaattt
     4861 cacaaataaa gcattttttt cactgcattc tagttgtggt ttgtccaaac tcatcaatgt
     4921 atcttatcat gtctgtatac cgtcgacctc tagctagagc ttggcgtaat catggtcata
     4981 gctgtttcct gtgtgaaatt gttatccgct cacaattcca cacaacatac gagccggaag
     5041 cataaagtgt aaagcctggg gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg
     5101 ctcactgccc gctttccagt cgggaaacct gtcgtgccag ctgcattaat gaatcggcca
     5161 acgcgcgggg agaggcggtt tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc
     5221 gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg cggtaatacg
     5281 gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa
     5341 ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc gcccccctga
     5401 cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag gactataaag
     5461 ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga ccctgccgct
     5521 taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc atagctcacg
     5581 ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc
     5641 ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt ccaacccggt
     5701 aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca gagcgaggta
     5761 tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca ctagaagaac
     5821 agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag ttggtagctc
     5881 ttgatccggc aaacaaacca ccgctggtag cggttttttt gtttgcaagc agcagattac
     5941 gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca
     6001 gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac
     6061 ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac
     6121 ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt
     6181 tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt
     6241 accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt
     6301 atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc
     6361 cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa
     6421 tagtttgcgc aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg
     6481 tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt
     6541 gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc
     6601 agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt
     6661 aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg
     6721 gcgaccgagt tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac
     6781 tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc
     6841 gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt
     6901 tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg
     6961 aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat attattgaag
     7021 catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa
     7081 acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtc
//