pAAVLINK-EF1alpha-HA-5'-ECPAS

LOCUS       pAAVLINK-EF1alpha-HA-5'-ECPAS            6608 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 6608)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 11, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..6608
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     promoter        158..700
                     /label=EF1-alpha
                     /note="Chimeric promoter of ef1a and htlv 5' UTR"
     promoter        187..398
                     /label=EF-1-alpha core promoter
                     /note="core promoter for human elongation factor 
                     EF-1-alpha"
     LTR             411..679
                     /label=5' LTR (truncated)
                     /note="truncated 5' long terminal repeat (LTR) from human 
                     T-cell leukemia virus (HTLV) type 1"
     misc_feature    505..510
                     /label=SDS
     CDS             716..742
                     /codon_start=1
                     /product="HA (human influenza hemagglutinin) epitope tag"
                     /label=HA
                     /translation="YPYDVPDYA"
     CDS             749..3748
                     /codon_start=1
                     /gene="ECPAS"
                     /gene_synonym="ECM29; KIAA0368"
                     /product="proteasome adapter and scaffold protein 
                     ECM29isoform 3"
                     /label=ECPAS
                     /note="isoform 3 is encoded by transcript variant 3;homolog
                     of yeast Ecm29; ECM29 homolog, proteasome accessory 
                     protein; proteasome-associated protein ECM29 homolog; ecm29
                     proteasome adapter and scaffold; proteasome adapter and 
                     scaffold protein ECM29"
                     /db_xref="GI:1428713215"
                     /db_xref="CCDS:CCDS94456.1"
                     /db_xref="GeneID:23392"
                     /db_xref="HGNC:HGNC:29020"
                     /db_xref="MIM:616694"
                     /protein_id="NP_001351858.1"
                     /translation="YHIDCRDQLERVFLRLGHAETDEQLQNIISKFLPPVLLKLSSTQE
                     GVRKKVMELLVHLNKRIKSRPKIQLPVETLLVQYQDPAAVSFVTNFTIIYVKMGYPRLP
                     VEKQCELAPTLLTAMEGKPQPQQDSLMHLLIPTLFHMKYPVESSKSASPFNLAEKPKTV
                     QLLLDFMLDVLLMPYGYVLNESQSRQNSSSAQGSSSNSGGGSGIPQPPPGMSFYAAKRV
                     IGDNPWTPEQLEQCKLGIVKFIEAEQVPELEAVLHLVIASSDTRHSVATAADLELKSKQ
                     SLIDWNNPAIINKMYKVYLGDIPLKTKEGAVLKPELKRDPVSTRVKLKIVPHLLRSRQA
                     AETFPANIQVVYDGLFGTNTNSKLRTLSLQFVHHICITCPEIKIKPLGPMLLNGLTKLI
                     NEYKEDPKLLSMAYSAVGKLSSRMPHLFTKDIALVQQLFEALCKEEPETRLAIQEALSM
                     MVGAYSTLEGAQRTLMEALVASYLIKPEVQVRQVAVKFASTVFPSDHIPSRYLLLLAAG
                     DPREEVHGEAQRVLRCLPGRNRKESTSEQMPSFPEMVYYIQEKASHRMKTPVKYMTGTT
                     VLPFNPAAFGEIVLYLRMCLAHSAGVVPTSQSLADMQDHAPAIGRYIRTLMSSGQMAPS
                     SSNKSGETNPVQIYIGLLQQLLAGVGGLPVMYCLLEAVSVYPEKLATKFVDKTEWIKSL
                     MNSSKEEMRELAALFYSVVVSTVSGNELKSMIEQLIKTTKDNHSPEIQHGSLLALGFTV
                     GRYLAKKKMRMSEQQDLERNADTLPDQEELIQSATETIGSFLDSTSPLLAIAACTALGE
                     IGRNGPLPIPSEGSGFTKLHLVESLLSRIPSSKETNKMKERAIQTLGYFPVGDGDFPHQ
                     KLLLQGLMDSVEAKQIELQFTIGEAITSAAIGTSSVAARDAWQMTEEEYTPPAGAKVND
                     VVPWVLDVILNKHIISPNPHVRQAACIWLLSLVRKLSTHKEVKSHLKEIQSAFVSVLSE
                     NDELSQDVASK"
     misc_feature    848..853
                     /label=SDS
     misc_feature    1376..1381
                     /label=SDS
     misc_feature    3148..3153
                     /label=SDS
     intron          3749..3789
                     /label=Splicing Donor
     misc_feature    3793..3826
                     /label=lox2272-JT1571
     repeat_region   3871..4011
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    3871..4000
                     /label=AAV2 ITR
     repeat_region   3872..4012
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4086..4541
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        4823..4927
                     /gene="bla"
                     /label=AmpR promoter
     CDS             4928..5788
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      5959..6547
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtcgac tctagaggat ctgcgatcgc tccggtgccc
      181 gtcagtgggc agagcgcaca tcgcccacag tccccgagaa gttgggggga ggggtcggca
      241 attgaacggg tgcctagaga aggtggcgcg gggtaaactg ggaaagtgat gtcgtgtact
      301 ggctccgcct ttttcccgag ggtgggggag aaccgtatat aagtgcagta gtcgccgtga
      361 acgttctttt tcgcaacggg tttgccgcca gaacacagct gaagcttcga ggggctcgca
      421 tctctccttc acgcgcccgc cgccctacct gaggccgcca tccacgccgg ttgagtcgcg
      481 ttctgccgcc tcccgcctgt ggtgcctcct gaactgcgtc cgccgtctag gtaagtttaa
      541 agctcaggtc gagaccgggc ctttgtccgg cgctcccttg gagcctacct agactcagcc
      601 ggctctccac gctttgcctg accctgcttg ctcaactcta cgtctttgtt tcgttttctg
      661 ttctgcgccg ttacagatcc aagctgtgac cggcgcctac cgtacggcca ccatgtaccc
      721 ttatgacgta cctgactatg ctggatctta tcacattgat tgtagagatc agcttgaacg
      781 ggtcttttta cgacttggcc atgctgaaac agatgaacaa ttacagaata ttatatctaa
      841 attccttcct cctgttttgc tcaaactctc tagcacccaa gaaggagtac gtaaaaaggt
      901 aatggaactg ctggtccatc tgaataaacg tataaaaagc cgccccaaaa tacaacttcc
      961 agtagagaca ctgttggttc agtaccagga ccctgctgca gtttcctttg tcacaaattt
     1021 tactataatt tatgttaaaa tgggctatcc tcgcctacca gtggaaaaac aatgtgaact
     1081 ggcccctacg cttcttactg ccatggaagg gaagcctcag ccacagcagg atagcttaat
     1141 gcatctttta ataccaaccc tttttcacat gaaataccct gttgaatcat caaaatcagc
     1201 ttctccattt aatcttgctg agaaaccaaa gactgtgcag ctgcttttgg acttcatgct
     1261 agatgtcctt ctgatgcctt atggttacgt gttaaatgaa tcccagagtc gccaaaattc
     1321 atcttcagca cagggttctt cttcaaacag tggcggaggt tctggaatcc cacagcctcc
     1381 tccgggaatg agcttttatg cagccaaacg agttattggt gataacccat ggacacctga
     1441 acaattggaa cagtgcaaat tgggaatcgt gaaattcata gaagctgaac aggtgcctga
     1501 acttgaagct gttctccact tggtgattgc ctctagtgat acacgccaca gtgtggcaac
     1561 ggcagcagac ctggaattga aaagcaaaca gagcttaatt gactggaata atcctgccat
     1621 cattaataag atgtacaagg tgtaccttgg agatatacca ctgaagacaa aagagggtgc
     1681 agttctgaag ccagagttga aaagggaccc tgtcagtaca agagtcaagt taaagattgt
     1741 cccccatctc ctccgctcta gacaagctgc tgaaacgttc ccagccaaca ttcaggtggt
     1801 gtatgatgga ctttttggta caaatacaaa ttcaaagtta agaacattat ccctgcaatt
     1861 tgtgcatcat atttgtataa cctgtccaga aatcaagatt aagccattag gtccaatgct
     1921 tttgaatggc ctcaccaagc taatcaatga atacaaagag gaccctaaac tactgtcaat
     1981 ggcatattca gctgttggaa aactctccag tcggatgcca catttattca ctaaggatat
     2041 agcgcttgtg cagcagcttt ttgaagccct ttgcaaggaa gagcctgaga ctcgacttgc
     2101 tattcaagaa gctttatcta tgatggttgg agcgtatagt actttggaag gggcacagcg
     2161 aactctcatg gaggcacttg tggcttcgta cttaataaag cctgaagttc aagttcgaca
     2221 agtggctgtg aaatttgcca gtacggtgtt tccctcagat catatccctt ccagatattt
     2281 gctgctactg gctgcaggag atccacgtga agaagttcat ggagaagcac aacgcgtatt
     2341 aaggtgtctt ccaggtagaa acagaaaaga aagtacttct gagcagatgc cttccttccc
     2401 agaaatggtt tattacatcc aagaaaaggc ttctcatcga atgaaaactc cagtcaagta
     2461 catgaccggg accactgtcc ttccatttaa cccagcagcc tttggagaga tcgttctgta
     2521 cttgcgcatg tgccttgcgc acagtgcggg ggtggtgccc acctctcaga gtttggctga
     2581 tatgcaggat catgccccag ccattgggcg ctacatacgg actttaatgt caagcgggca
     2641 gatggcaccc tcatcatcta acaagagtgg ggagactaac cctgtccaga tctacattgg
     2701 cctgcttcag cagctgttag caggtgttgg aggtttgccg gttatgtact gtctattgga
     2761 agctgtgtca gtgtatccag aaaagctggc taccaaattt gtagacaaaa cagaatggat
     2821 aaagagtctg atgaatagca gtaaagaaga aatgcgcgaa ctggcagcgt tgttttattc
     2881 tgtagtggta tcaacagtgt cggggaatga gttgaaatca atgatagaac agcttataaa
     2941 gactacaaaa gacaatcaca gcccggagat acagcatgga tccttgcttg cattgggatt
     3001 cacggtggga aggtatttgg ctaaaaagaa aatgagaatg tcagagcaac aagacctgga
     3061 gagaaatgct gacaccctcc ctgatcaaga ggaactcatt cagagtgcta cagaaacaat
     3121 aggctcattt ttggacagta catcacccct cctggcaatt gctgcctgca cagccctggg
     3181 tgaaattggc agaaatggtc cacttccaat ccccagtgag ggatctggct ttaccaaatt
     3241 gcatcttgta gaaagcttac taagtagaat accttccagt aaagaaacaa ataagatgaa
     3301 agaacgagca atccaaacac tgggatattt tccagttggg gatggagatt ttcctcacca
     3361 gaaactcctc ttgcaaggtc tgatggattc tgtggaggcc aagcagatag aacttcagtt
     3421 cactattggc gaagccatta ccagtgctgc aataggaact agttctgtgg ctgcccgaga
     3481 tgcctggcaa atgactgaag aggaatatac tccacctgct ggagccaaag tgaatgatgt
     3541 ggttccatgg gtgttggatg tgattttaaa taaacatatc atcagcccca acccacacgt
     3601 gaggcaagca gcctgcatct ggctcctttc ccttgtcagg aagctaagta cccacaaaga
     3661 agtgaagtct catcttaaag aaattcaaag tgcatttgtt tcagttctat cagaaaatga
     3721 tgaacttagc caagatgttg catcaaaggt aagtatcaag gttacaagac aggtttaagg
     3781 agaccaataa gctattattc gtataggata ctttatacga agttattaag ttatggagac
     3841 cggtgtggca cgtgcggacc gagcggccgc aggaacccct agtgatggag ttggccactc
     3901 cctctctgcg cgctcgctcg ctcactgagg ccgggcgacc aaaggtcgcc cgacgcccgg
     3961 gctttgcccg ggcggcctca gtgagcgagc gagcgcgcag ctgcctgcag gggcgcctga
     4021 tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcatacgt caaagcaacc
     4081 atagtacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     4141 gaccgctaca cttgccagcg ccttagcgcc cgctcctttc gctttcttcc cttcctttct
     4201 cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     4261 atttagtgct ttacggcacc tcgaccccaa aaaacttgat ttgggtgatg gttcacgtag
     4321 tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     4381 tagtggactc ttgttccaaa ctggaacaac actcaactct atctcgggct attcttttga
     4441 tttataaggg attttgccga tttcggtcta ttggttaaaa aatgagctga tttaacaaaa
     4501 atttaacgcg aattttaaca aaatattaac gtttacaatt ttatggtgca ctctcagtac
     4561 aatctgctct gatgccgcat agttaagcca gccccgacac ccgccaacac ccgctgacgc
     4621 gccctgacgg gcttgtctgc tcccggcatc cgcttacaga caagctgtga ccgtctccgg
     4681 gagctgcatg tgtcagaggt tttcaccgtc atcaccgaaa cgcgcgagac gaaagggcct
     4741 cgtgatacgc ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg
     4801 tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc
     4861 aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag
     4921 gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg
     4981 ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt
     5041 gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt
     5101 tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt
     5161 attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa
     5221 tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag
     5281 agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac
     5341 aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac
     5401 tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac
     5461 cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac
     5521 tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact
     5581 tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg
     5641 tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt
     5701 tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat
     5761 aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta
     5821 gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa
     5881 tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga
     5941 aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac
     6001 aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt
     6061 tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc tagtgtagcc
     6121 gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat
     6181 cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag
     6241 acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc
     6301 cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag
     6361 cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac
     6421 aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg
     6481 gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct
     6541 atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc
     6601 tcacatgt
//