| 质粒名称: | pAAVLINK-EF1a-HA-FLNA-A |
|---|---|
| 出品公司: | Wekwikgene |
| 目录编号: | 0001751 |
| 产品用途: | 5' AAVLINK plasmid for FLNA expression |
| 存储实验室: | Zhonghua Lu Lab |
|
载体骨架基本信息 |
|
| 载体名称: | pAAV-MCS2 |
| 表达系统: | 腺病毒 |
| 启动子: | EF1a |
| 拷贝数: | 高拷贝 |
| 载体抗性: | 氨苄霉素 |
| 生长温度: | 37 ℃ |
| 宿主菌: | DH5alpha |
|
插入基因信息 |
|
| 插入基因名称: | FLNA |
| 物种来源: | H.sapiens |
| 密码子优化: | 未优化 |
|
文献引用方法 |
|
| 参考文献部分: |
AAVLINK: A Potent DNA-Recombination Method for Large Cargo Delivery in Gene Therapy
Jianbang Lin, Yunping Lin, Sijia Wen, Wenhua Cao, Jianqing Zhang, Yujing Zhang, Zexuan Hong, Wenhui Liao, Yunyi Lin, Hanhe Liu, Qiwei Liu, Qi Li, Baiming Chen, Mengqi Li, Ziwei Luo, Luyu Yang, Yi Yang, Stephanie H. Zheng, Youcui Wang, Hongyuan Chu, Yu Hu, Yifan Qin, Brooke X. Luo, Shiyu Tian, Yefei Chen, Ting Yan, Lixin Yang, Hong Wang, Taian Liu, Yuwu Jiang, and Zhonghua Lu.
Under Review
|
pAAVLINK-EF1a-HA-FLNA-A
价格：元
规格： 2ug质粒（仅用于转化提取） 转染级去内毒素液体即用质粒100μg 转染级去内毒素液体即用质粒500μg 转染级去内毒素液体即用质粒1mg 更大规格-质粒构建-质粒定制-请联系我们
联系方式：I47-825O-882O
买家导航
pAAVLINK-EF1a-HA-FLNA-A载体质粒基本信息
pAAVLINK-EF1a-HA-FLNA-A质粒图谱载体图谱和pAAVLINK-EF1a-HA-FLNA-A载体序列质粒序列多克隆位点信息
pAAVLINK-EF1a-HA-FLNA-A质粒载体简介
pAAVLINK-EF1a-HA-FLNA-A质粒序列载体序列
LOCUS pAAVLINK-EF1a-HA-FLNA-A 6422 bp DNA circular SYN 21-NOV-2025
DEFINITION synthetic circular DNA
ACCESSION .
VERSION .
KEYWORDS .
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6422)
AUTHORS Stavrini Papadaki
TITLE Direct Submission
JOURNAL Exported Jun 5, 2026 from SnapGene 6.0.2
https://www.biofeng.com
FEATURES Location/Qualifiers
source 1..6422
/mol_type="other DNA"
/organism="synthetic DNA construct"
repeat_region 1..130
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 12..130
/label=AAV2 ITR
promoter 146..688
/label=EF1-alpha
/note="Chimeric promoter of ef1a and htlv 5' UTR"
promoter 175..386
/label=EF-1-alpha core promoter
/note="core promoter for human elongation factor
EF-1-alpha"
LTR 399..667
/label=5' LTR (truncated)
/note="truncated 5' long terminal repeat (LTR) from human
T-cell leukemia virus (HTLV) type 1"
misc_feature 493..498
/label=SDS
CDS 704..730
/codon_start=1
/product="HA (human influenza hemagglutinin) epitope tag"
/label=HA
/translation="YPYDVPDYA"
CDS 737..3562
/codon_start=1
/label=FLNA
/translation="MSSSHSRAGQSAAGAAPGGGVDTRDAEMPATEKDLAEDAPWKKIQ
QNTFTRWCNEHLKCVSKRIANLQTDLSDGLRLIALLEVLSQKKMHRKHNQRPTFRQMQL
ENVSVALEFLDRESIKLVSIDSKAIVDGNLKLILGLIWTLILHYSISMPMWDEEEDEEA
KKQTPKQRLLGWIQNKLPQLPITNFSRDWQSGRALGALVDSCAPGLCPDWDSWDASKPV
TNAREAMQQADDWLGIPQVITPEEIVDPNVDEHSVMTYLSQFPKAKLKPGAPLRPKLNP
KKARAYGPGIEPTGNMVKKRAEFTVETRSAGQGEVLVYVEDPAGHQEEAKVTANNDKNR
TFSVWYVPEVTGTHKVTVLFAGQHIAKSPFEVYVDKSQGDASKVTAQGPGLEPSGNIAN
KTTYFEIFTAGAGTGEVEVVIQDPMGQKGMVEPQLEARGDSTYRCSYQPTMEGVHTVHV
TFAGVPIPRSPYTVTVGQACNPSACRAVGRGLQPKGVRVKETADFKVYTKGAGSGELKV
TVKGPKGEERVKQKDLGDGVYGFEYYPMVPGTYIVTITWGGQNIGRSPFEVKVGTECGN
QKVRAWGPGLEGGVVGKSADFVVEAIGDDVGTLGFSVEGPSQAKIECDDKGDGSCDVRY
WPQEAGEYAVHVLCNSEDIRLSPFMADIRDAPQDFHPDRVKARGPGLEKTGVAVNKPAE
FTVDAKHGGKAPLRVQVQDNEGCPVEALVKDNGNGTYSCSYVPRKPVKHTAMVSWGGVS
IPNSPFRVNVGAGSHPNKVKVYGPGVAKTGLKAHEPTYFTVDCAEAGQGDVSIGIKCAP
GVVGPAEADIDFDIIRNDNDTFTVKYTPRGAGSYTIMVLFADQATPTSPIRVKVEPSHD
ASKVKAEGPGLSRTGVELGKPTHFTVNAKAAGKGKLDVQFSGLTKGDAVRDVDIIDHHD
NTYTVKYTPVQQ"
misc_feature 1206..1211
/label=SDS
misc_feature 1209..1214
/label=SDS
misc_feature 1218..1223
/label=SDS
misc_feature 1716..1721
/label=SDS
misc_feature 1719..1724
/label=SDS
misc_feature 2649..2654
/label=SDS
intron 3563..3603
/label=Splicing Donor
misc_feature 3607..3640
/label=loxP-JT1571
repeat_region 3685..3825
/label=AAV2 ITR
/note="inverted terminal repeat of adeno-associated virus
serotype 2"
misc_feature 3685..3814
/label=AAV2 ITR
rep_origin 3900..4355
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 4637..4741
/gene="bla"
/label=AmpR promoter
CDS 4742..5602
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 5773..6361
/direction=RIGHT
/label=ori
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
ORIGIN
1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
121 aggggttcct gcggccgcac gcgtggatct gcgatcgctc cggtgcccgt cagtgggcag
181 agcgcacatc gcccacagtc cccgagaagt tggggggagg ggtcggcaat tgaacgggtg
241 cctagagaag gtggcgcggg gtaaactggg aaagtgatgt cgtgtactgg ctccgccttt
301 ttcccgaggg tgggggagaa ccgtatataa gtgcagtagt cgccgtgaac gttctttttc
361 gcaacgggtt tgccgccaga acacagctga agcttcgagg ggctcgcatc tctccttcac
421 gcgcccgccg ccctacctga ggccgccatc cacgccggtt gagtcgcgtt ctgccgcctc
481 ccgcctgtgg tgcctcctga actgcgtccg ccgtctaggt aagtttaaag ctcaggtcga
541 gaccgggcct ttgtccggcg ctcccttgga gcctacctag actcagccgg ctctccacgc
601 tttgcctgac cctgcttgct caactctacg tctttgtttc gttttctgtt ctgcgccgtt
661 acagatccaa gctgtgaccg gcgcctaccg tacggccacc atgtaccctt atgacgtacc
721 tgactatgct ggatctatga gtagctccca ctctcgggcg ggccagagcg cagcaggcgc
781 ggctccgggc ggcggcgtcg acacgcggga cgccgagatg ccggccaccg agaaggacct
841 ggcggaggac gcgccgtgga agaagatcca gcagaacact ttcacgcgct ggtgcaacga
901 gcacctgaag tgcgtgagca agcgcatcgc caacctgcag acggacctga gcgacgggct
961 gcggcttatc gcgctgttgg aggtgctcag ccagaagaag atgcaccgca agcacaacca
1021 gcggcccact ttccgccaaa tgcagcttga gaacgtgtcg gtggcgctcg agttcctgga
1081 ccgcgagagc atcaaactgg tgtccatcga cagcaaggcc atcgtggacg ggaacctgaa
1141 gctgatcctg ggcctcatct ggaccctgat cctgcactac tccatctcca tgcccatgtg
1201 ggacgaggag gaggatgagg aggccaagaa gcagaccccc aagcagaggc tcctgggctg
1261 gatccagaac aagctgccgc agctgcccat caccaacttc agccgggact ggcagagcgg
1321 ccgggccctg ggcgccctgg tggacagctg tgccccgggc ctgtgtcctg actgggactc
1381 ttgggacgcc agcaagcccg ttaccaatgc gcgagaggcc atgcagcagg cggatgactg
1441 gctgggcatc ccccaggtga tcacccccga ggagattgtg gaccccaacg tggacgagca
1501 ctctgtcatg acctacctgt cccagttccc caaggccaag ctgaagccag gggctccctt
1561 gcggcccaaa ctgaacccga agaaagcccg tgcctacggg ccaggcatcg agcccacagg
1621 caacatggtg aagaagcggg cagagttcac tgtggagacc agaagtgctg gccagggaga
1681 ggtgctggtg tacgtggagg acccggccgg acaccaggag gaggcaaaag tgaccgccaa
1741 taacgacaag aaccgcacct tctccgtctg gtacgtcccc gaggtgacgg ggactcataa
1801 ggttactgtg ctctttgctg gccagcacat cgccaagagc cccttcgagg tgtacgtgga
1861 taagtcacag ggtgacgcca gcaaagtgac agcccaaggt cccggcctgg agcccagtgg
1921 caacatcgcc aacaagacca cctactttga gatctttacg gcaggagctg gcacgggcga
1981 ggtcgaggtt gtgatccagg accccatggg acagaagggc atggtagagc ctcagctgga
2041 ggcccggggc gacagcacat accgctgcag ctaccagccc accatggagg gcgtccacac
2101 cgtgcacgtc acgtttgccg gcgtgcccat ccctcgcagc ccctacactg tcactgttgg
2161 ccaagcctgt aacccgagtg cctgccgggc ggttggccgg ggcctccagc ccaagggtgt
2221 gcgggtgaag gagacagctg acttcaaggt gtacacaaag ggcgctggca gtggggagct
2281 gaaggtcacc gtgaagggcc ccaagggaga ggagcgcgtg aagcagaagg acctggggga
2341 tggcgtgtat ggcttcgagt attaccccat ggtccctgga acctatatcg tcaccatcac
2401 gtggggtggt cagaacatcg ggcgcagtcc cttcgaagtg aaggtgggca ccgagtgtgg
2461 caatcagaag gtacgggcct ggggccctgg gctggagggc ggcgtcgttg gcaagtcagc
2521 agactttgtg gtggaggcta tcggggacga cgtgggcacg ctgggcttct cggtggaagg
2581 gccatcgcag gctaagatcg aatgtgacga caagggcgac ggctcctgtg atgtgcgcta
2641 ctggccgcag gaggctggcg agtatgccgt tcacgtgctg tgcaacagcg aagacatccg
2701 cctcagcccc ttcatggctg acatccgtga cgcgccccag gacttccacc cagacagggt
2761 gaaggcacgt gggcctggat tggagaagac aggtgtggcc gtcaacaagc cagcagagtt
2821 cacagtggat gccaagcacg gtggcaaggc cccacttcgg gtccaagtcc aggacaatga
2881 aggctgccct gtggaggcgt tggtcaagga caacggcaat ggcacttaca gctgctccta
2941 cgtgcccagg aagccggtga agcacacagc catggtgtcc tggggaggcg tcagcatccc
3001 caacagcccc ttcagggtga atgtgggagc tggcagccac cccaacaagg tcaaagtata
3061 cggccccgga gtagccaaga cagggctcaa ggcccacgag cccacctact tcactgtgga
3121 ctgcgccgag gctggccagg gggacgtcag catcggcatc aagtgtgccc ctggagtggt
3181 aggccccgcc gaagctgaca tcgacttcga catcatccgc aatgacaatg acaccttcac
3241 ggtcaagtac acgccccggg gggctggcag ctacaccatt atggtcctct ttgctgacca
3301 ggccacgccc accagcccca tccgagtcaa ggtggagccc tctcatgacg ccagtaaggt
3361 gaaggccgag ggccctggcc tcagtcgcac tggtgtcgag cttggcaagc ccacccactt
3421 cacagtaaat gccaaagctg ctggcaaagg caagctggac gtccagttct caggactcac
3481 caagggggat gcagtgcgag atgtggacat catcgaccac catgacaaca cctacacagt
3541 caagtacacg cctgtccagc aggtaagtat caaggttaca agacaggttt aaggagacca
3601 ataagctatt attcgtatag catacattat acgaagttat taagttatgg agaccggtgt
3661 ggcacgtgcg gaccgagcgg ccgcaggaac ccctagtgat ggagttggcc actccctctc
3721 tgcgcgctcg ctcgctcact gaggccgggc gaccaaaggt cgcccgacgc ccgggctttg
3781 cccgggcggc ctcagtgagc gagcgagcgc gcagctgcct gcaggggcgc ctgatgcggt
3841 attttctcct tacgcatctg tgcggtattt cacaccgcat acgtcaaagc aaccatagta
3901 cgcgccctgt agcggcgcat taagcgcggc gggtgtggtg gttacgcgca gcgtgaccgc
3961 tacacttgcc agcgccttag cgcccgctcc tttcgctttc ttcccttcct ttctcgccac
4021 gttcgccggc tttccccgtc aagctctaaa tcgggggctc cctttagggt tccgatttag
4081 tgctttacgg cacctcgacc ccaaaaaact tgatttgggt gatggttcac gtagtgggcc
4141 atcgccctga tagacggttt ttcgcccttt gacgttggag tccacgttct ttaatagtgg
4201 actcttgttc caaactggaa caacactcaa ctctatctcg ggctattctt ttgatttata
4261 agggattttg ccgatttcgg tctattggtt aaaaaatgag ctgatttaac aaaaatttaa
4321 cgcgaatttt aacaaaatat taacgtttac aattttatgg tgcactctca gtacaatctg
4381 ctctgatgcc gcatagttaa gccagccccg acacccgcca acacccgctg acgcgccctg
4441 acgggcttgt ctgctcccgg catccgctta cagacaagct gtgaccgtct ccgggagctg
4501 catgtgtcag aggttttcac cgtcatcacc gaaacgcgcg agacgaaagg gcctcgtgat
4561 acgcctattt ttataggtta atgtcatgat aataatggtt tcttagacgt caggtggcac
4621 ttttcgggga aatgtgcgcg gaacccctat ttgtttattt ttctaaatac attcaaatat
4681 gtatccgctc atgagacaat aaccctgata aatgcttcaa taatattgaa aaaggaagag
4741 tatgagtatt caacatttcc gtgtcgccct tattcccttt tttgcggcat tttgccttcc
4801 tgtttttgct cacccagaaa cgctggtgaa agtaaaagat gctgaagatc agttgggtgc
4861 acgagtgggt tacatcgaac tggatctcaa cagcggtaag atccttgaga gttttcgccc
4921 cgaagaacgt tttccaatga tgagcacttt taaagttctg ctatgtggcg cggtattatc
4981 ccgtattgac gccgggcaag agcaactcgg tcgccgcata cactattctc agaatgactt
5041 ggttgagtac tcaccagtca cagaaaagca tcttacggat ggcatgacag taagagaatt
5101 atgcagtgct gccataacca tgagtgataa cactgcggcc aacttacttc tgacaacgat
5161 cggaggaccg aaggagctaa ccgctttttt gcacaacatg ggggatcatg taactcgcct
5221 tgatcgttgg gaaccggagc tgaatgaagc cataccaaac gacgagcgtg acaccacgat
5281 gcctgtagca atggcaacaa cgttgcgcaa actattaact ggcgaactac ttactctagc
5341 ttcccggcaa caattaatag actggatgga ggcggataaa gttgcaggac cacttctgcg
5401 ctcggccctt ccggctggct ggtttattgc tgataaatct ggagccggtg agcgtgggtc
5461 tcgcggtatc attgcagcac tggggccaga tggtaagccc tcccgtatcg tagttatcta
5521 cacgacgggg agtcaggcaa ctatggatga acgaaataga cagatcgctg agataggtgc
5581 ctcactgatt aagcattggt aactgtcaga ccaagtttac tcatatatac tttagattga
5641 tttaaaactt catttttaat ttaaaaggat ctaggtgaag atcctttttg ataatctcat
5701 gaccaaaatc ccttaacgtg agttttcgtt ccactgagcg tcagaccccg tagaaaagat
5761 caaaggatct tcttgagatc ctttttttct gcgcgtaatc tgctgcttgc aaacaaaaaa
5821 accaccgcta ccagcggtgg tttgtttgcc ggatcaagag ctaccaactc tttttccgaa
5881 ggtaactggc ttcagcagag cgcagatacc aaatactgtt cttctagtgt agccgtagtt
5941 aggccaccac ttcaagaact ctgtagcacc gcctacatac ctcgctctgc taatcctgtt
6001 accagtggct gctgccagtg gcgataagtc gtgtcttacc gggttggact caagacgata
6061 gttaccggat aaggcgcagc ggtcgggctg aacggggggt tcgtgcacac agcccagctt
6121 ggagcgaacg acctacaccg aactgagata cctacagcgt gagctatgag aaagcgccac
6181 gcttcccgaa gggagaaagg cggacaggta tccggtaagc ggcagggtcg gaacaggaga
6241 gcgcacgagg gagcttccag ggggaaacgc ctggtatctt tatagtcctg tcgggtttcg
6301 ccacctctga cttgagcgtc gatttttgtg atgctcgtca ggggggcgga gcctatggaa
6361 aaacgccagc aacgcggcct ttttacggtt cctggccttt tgctggcctt ttgctcacat
6421 gt
//
