pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEO1-FLAG

LOCUS       pAAVLINKv2-SCP1-Cre-UDeg3a-3'-NEO1-FLAG         7173 bp DNA     circular SYN 24-NOV-2025
DEFINITION  synthetic circular DNA
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 7173)
  AUTHORS   Stavrini Papadaki
  TITLE     Direct Submission
  JOURNAL   Exported Jun 10, 2026 from SnapGene 6.0.2
            https://www.biofeng.com
FEATURES             Location/Qualifiers
     source          1..7173
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     repeat_region   1..130
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
                     /note="color: #ffe4c4"
     misc_feature    145..225
                     /label=SCP1
     CDS             237..1265
                     /codon_start=1
                     /product="site-specific recombinase"
                     /label=Cre
                     /note="Cre is a site-specific recombinase from 
                     bacteriophage P1. Recombination occurs at loxP sequences."
                     /translation="MSNLLTVHQNLPALPVDATSDEVRKNLMDMFRDRQAFSEHTWKML
                     LSVCRSWAAWCKLNNRKWFPAEPEDVRDYLLYLQARGLAVKTIQQHLGQLNMLHRRSGL
                     PRPSDSNAVSLVMRRIRKENVDAGERAKQALAFERTDFDQVRSLMENSDRCQDIRNLAF
                     LGIAYNTLLRIAEIARIRVKDISRTDGGRMLIHIGRTKTLVSTAGVEKALSLGVTKLVE
                     RWISVSGVADDPNNYLFCRVRKNGVAAPSATSQLSTRALEGIFEATHRLIYGAKDDSGQ
                     RYLAWSGHSARVGAARDMARAGVSIPEIMQAGGWTNVNIVMNYIRNLDSETGAMVRLLE
                     DGD"
     CDS             1281..1370
                     /codon_start=1
                     /label=UDeg3a
                     /translation="LLLKLLLLGGGSGPRPRGTRGKGRRIHYKG"
     misc_feature    1295..1300
                     /label=SDS
     misc_feature    1298..1303
                     /label=SDS
     intron          1374..1414
                     /label=Splicing Donor
     protein_bind    1421..1454
                     /label=lox2272-JTZ17
                     /bound_moiety="Cre recombinase"
                     /note="Right element (RE) mutant of loxP (Araki et al., 
                     2010). Cre-mediated recombination occurs in the 8-bp core 
                     sequence (GCATACAT)."
     intron          1455..1546
                     /label=Splicing Acceptor
                     /note="Splicing acceptor"
     CDS             1547..3837
                     /codon_start=1
                     /gene="NEO1"
                     /gene_synonym="IGDCC2; NGN; NTN1R2"
                     /product="neogenin isoform 1 precursor"
                     /label=NEO1
                     /note="isoform 1 precursor is encoded by transcriptvariant 
                     1; immunoglobulin superfamily DCC subclass member 2; 
                     neogenin homolog 1"
                     /db_xref="GI:157311649"
                     /db_xref="CCDS:CCDS10247.1"
                     /db_xref="GeneID:4756"
                     /db_xref="HGNC:HGNC:7754"
                     /db_xref="MIM:601907"
                     /protein_id="NP_002490.2"
                     /translation="LDRGTEYNFRVAALTINGTGPATDWLSAETFESDLDETRVPEVPS
                     SLHVRPLVTSIVVSWTPPENQNIVVRGYAIGYGIGSPHAQTIKVDYKQRYYTIENLDPS
                     SHYVITLKAFNNVGEGIPLYESAVTRPHTDTSEVDLFVINAPYTPVPDPTPMMPPVGVQ
                     ASILSHDTIRITWADNSLPKHQKITDSRYYTVRWKTNIPANTKYKNANATTLSYLVTGL
                     KPNTLYEFSVMVTKGRRSSTWSMTAHGTTFELVPTSPPKDVTVVSKEGKPKTIIVNWQP
                     PSEANGKITGYIIYYSTDVNAEIHDWVIEPVVGNRLTHQIQELTLDTPYYFKIQARNSK
                     GMGPMSEAVQFRTPKADSSDKMPNDQASGSGGKGSRLPDLGSDYKPPMSGSNSPHGSPT
                     SPLDSNMLLVIIVSVGVITIVVVVIIAVFCTRRTTSHQKKKRAACKSVNGSHKYKGNSK
                     DVKPPDLWIHHERLELKPIDKSPDPNPIMTDTPIPRNSQDITPVDNSMDSNIHQRRNSY
                     RGHESEDSMSTLAGRRGMRPKMMMPFDSQPPQPVISAHPIHSLDNPHHHFHSSSLASPA
                     RSHLYHPGSPWPIGTSMSLSDRANSTESVRNTPSTDTMPASSSQTCCTDHQDPEGATSS
                     SYLASSQEEDSGQSLPTAHVRPSHPLKSFAVPAIPPPGPPTYDPALPSTPLLSQQALNH
                     HIHSVKTASIGTLGRSRPPMPVVVPSAPEVQETTRMLEDSESSYEPDELTKEMAHLEGL
                     MKDLNAITTA"
     misc_feature    3682..3687
                     /label=SDS
     CDS             3844..3909
                     /codon_start=1
                     /product="three tandem FLAG(R) epitope tags, followed by an
                     enterokinase cleavage site"
                     /label=3xFLAG
                     /translation="DYKDHDGDYKDHDIDYKDDDDK"
     terminator      3913..4393
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     misc_feature    4057..4062
                     /label=SDS
     terminator      4215..4263
                     /label=W3SL
                     /note="Short WPRE and SV40 polyA"
     repeat_region   4436..4576
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     misc_feature    4436..4565
                     /label=AAV2 ITR
     repeat_region   4437..4577
                     /label=AAV2 ITR
                     /note="inverted terminal repeat of adeno-associated virus 
                     serotype 2"
     rep_origin      4651..5106
                     /direction=RIGHT
                     /label=f1 ori
                     /note="f1 bacteriophage origin of replication; arrow 
                     indicates direction of (+) strand synthesis"
     promoter        5388..5492
                     /gene="bla"
                     /label=AmpR promoter
     CDS             5493..6353
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     rep_origin      6524..7112
                     /direction=RIGHT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
ORIGIN
        1 cctgcaggca gctgcgcgct cgctcgctca ctgaggccgc ccgggcgtcg ggcgaccttt
       61 ggtcgcccgg cctcagtgag cgagcgagcg cgcagagagg gagtggccaa ctccatcact
      121 aggggttcct gcggccgcac gcgtgtactt atataagggg gtgggggcgc gttcgtcctc
      181 agtcgcgatc gaacactcga gccgagcaga cgtgcctacg gaccgcgtac gccaccatgt
      241 ccaatttact gaccgtacac caaaatttgc ctgcattacc ggtcgatgca acgagtgatg
      301 aggttcgcaa gaacctgatg gacatgttca gggatcgcca ggcgttttct gagcatacct
      361 ggaaaatgct tctgtccgtt tgccggtcgt gggcggcatg gtgcaagttg aataaccgga
      421 aatggtttcc cgcagaacct gaagatgttc gcgattatct tctatatctt caggcgcgcg
      481 gtctggcagt aaaaaccatc cagcaacatt tgggccagct aaacatgctt catcgtcggt
      541 ccgggctgcc acgaccaagt gacagcaatg ctgtttcact ggttatgcgg cggatccgaa
      601 aagaaaacgt tgatgccggt gaacgtgcaa aacaggctct agcgttcgaa cgcactgatt
      661 tcgaccaggt tcgttcactc atggaaaata gcgatcgctg ccaggatata cgtaatctgg
      721 catttctggg gattgcttat aacaccctgt tacgtatagc cgaaattgcc aggatcaggg
      781 ttaaagatat ctcacgtact gacggtggga gaatgttaat ccatattggc agaacgaaaa
      841 cgctggttag caccgcaggt gtagagaagg cacttagcct gggggtaact aaactggtcg
      901 agcgatggat ttccgtctct ggtgtagctg atgatccgaa taactacctg ttttgccggg
      961 tcagaaaaaa tggtgttgcc gcgccatctg ccaccagcca gctatcaact cgcgccctgg
     1021 aagggatttt tgaagcaact catcgattga tttacggcgc taaggatgac tctggtcaga
     1081 gatacctggc ctggtctgga cacagtgccc gtgtcggagc cgcgcgagat atggcccgcg
     1141 ctggagtttc aataccggag atcatgcaag ctggtggctg gaccaatgta aatattgtaa
     1201 tgaactatat ccgtaacctg gatagtgaaa caggggcaat ggtgcgcctg ctggaagatg
     1261 gcgatggagg tggatctggt ctgctgctga aactcctcct cctcggtggt ggttctggtc
     1321 ctcgtccccg tggtactcgt ggtaaaggtc gccgtattca ctacaaagga taagtaagta
     1381 tcaaggttac aagacaggtt taaggagacc aataactagt ataacttcgt ataggatact
     1441 ttatagcaat ttatgaaact gggcttgtcg agacagagaa gactcttgcg tttctgatag
     1501 gcacctattg gtcttactga catccacttt gcctttctct ccacaggtct tgatcggggg
     1561 actgagtata atttccgagt ggctgctcta acaatcaatg gtacaggccc ggcaactgac
     1621 tggctgtctg ctgaaacttt tgaaagtgac ctagatgaaa ctcgtgttcc tgaagtgcct
     1681 agctctcttc acgtacgccc gctcgttact agcatcgtag tgagctggac tcctccagag
     1741 aatcagaaca ttgtggtcag aggttacgcc attggttatg gcattggcag ccctcatgcc
     1801 cagaccatca aagtggacta taaacagcgc tattacacca ttgaaaatct ggatcccagc
     1861 tctcactatg tgattaccct gaaagcattt aataacgtgg gtgaaggcat ccccctgtat
     1921 gagagtgctg tgaccaggcc tcacacagac acttctgaag ttgatttatt tgttattaat
     1981 gctccataca ctccagtgcc agatcccact cccatgatgc caccagtggg agttcaggct
     2041 tccattctga gtcatgacac catcaggatt acgtgggcag acaactcgct gcccaagcac
     2101 cagaagatta cagactcccg atactacacc gtccgatgga aaaccaacat cccagcaaac
     2161 accaagtaca agaatgcaaa tgcaaccact ttgagttatt tggtgactgg tttaaagccg
     2221 aatacactct atgaattctc tgtgatggtg accaaaggtc gaagatcaag tacatggagt
     2281 atgacagccc atgggaccac ctttgaatta gttccgactt ctccacccaa ggatgtgact
     2341 gttgtgagta aagaggggaa acctaagacc ataattgtga attggcagcc tccctccgaa
     2401 gccaatggca aaattacagg ttacatcata tattacagta cagatgtgaa tgcagagata
     2461 catgactggg ttattgagcc tgttgtggga aacagactga ctcaccagat acaagagtta
     2521 actcttgaca caccatacta cttcaaaatc caggcacgga actcaaaggg catgggaccc
     2581 atgtctgaag ctgtccaatt cagaacacct aaagcggact cctctgataa aatgcctaat
     2641 gatcaagcct cagggtctgg agggaaagga agccggctgc cagacctagg atccgactac
     2701 aaacctccaa tgagcggcag taacagccct catgggagcc ccacctctcc tctggacagt
     2761 aatatgctgc tggtcataat tgtttctgtt ggcgtcatca ccatcgtggt ggttgtgatt
     2821 atcgctgtct tttgtacccg tcgtaccacc tctcaccaga aaaagaaacg agctgcctgc
     2881 aaatcagtga atggctctca taagtacaaa gggaattcca aagatgtgaa acctccagat
     2941 ctctggatcc atcatgagag actggagctg aaacccattg ataagtctcc agacccaaac
     3001 cccatcatga ctgatactcc aattcctcgc aactctcaag atatcacacc agttgacaac
     3061 tccatggaca gcaatatcca tcaaaggcga aattcataca gagggcatga gtcagaggac
     3121 agcatgtcta cactggctgg aaggcgagga atgagaccaa aaatgatgat gccctttgac
     3181 tcccagccac cccagcctgt gattagtgcc catcccatcc attccctcga taaccctcac
     3241 catcatttcc actccagcag cctcgcttct ccagctcgca gtcatctcta ccacccgggc
     3301 agcccatggc ccattggcac atccatgtcc ctttcagaca gggccaattc cacagaatcc
     3361 gttcgaaata cccccagcac tgacaccatg ccagcctctt cgtctcaaac atgctgcact
     3421 gatcaccagg accctgaagg tgctaccagc tcctcttact tggccagctc ccaagaggaa
     3481 gattcaggcc agagtcttcc cactgcccat gttcgccctt cccacccatt gaagagcttc
     3541 gccgtgccag caatcccgcc tccaggacct cccacctatg atcctgcatt gccaagcaca
     3601 ccattactgt cccagcaagc tctgaaccat cacattcact cagtgaagac agcctccatc
     3661 gggactctag gaaggagccg gcctcctatg ccagtggttg ttcccagtgc ccctgaagtg
     3721 caggagacca caaggatgtt ggaagactcc gagagtagct atgaaccaga tgagctgacc
     3781 aaagagatgg cccacctgga aggactaatg aaggacctaa acgctatcac aacagcatgt
     3841 acagactaca aagaccatga cggtgattat aaagatcatg acatcgacta caaggatgac
     3901 gatgacaagt aactcgagat aatcaacctc tggattacaa aatttgtgaa agattgactg
     3961 gtattcttaa ctatgttgct ccttttacgc tatgtggata cgctgcttta atgcctttgt
     4021 atcatgctat tgcttcccgt atggctttca ttttctcctc cttgtataaa tcctggttag
     4081 ttcttgccac ggcggaactc atcgccgcct gccttgcccg ctgctggaca ggggctcggc
     4141 tgttgggcac tgacaattcc gtggtgttta tttgtgaaat ttgtgatgct attgctttat
     4201 ttgtaaccat ctagctttat ttgtgaaatt tgtgatgcta ttgctttatt tgtaaccatc
     4261 tagctttatt tgtgaaattt gtgatgctat tgctttattt gtaaccatta taagctgcaa
     4321 taaacaagtt aacaacaaca attgcattca ttttatgttt caggttcagg gggagatgtg
     4381 ggaggttttt taaagatctg gagaccggtg tggcacgtgc ggaccgagcg gccgcaggaa
     4441 cccctagtga tggagttggc cactccctct ctgcgcgctc gctcgctcac tgaggccggg
     4501 cgaccaaagg tcgcccgacg cccgggcttt gcccgggcgg cctcagtgag cgagcgagcg
     4561 cgcagctgcc tgcaggggcg cctgatgcgg tattttctcc ttacgcatct gtgcggtatt
     4621 tcacaccgca tacgtcaaag caaccatagt acgcgccctg tagcggcgca ttaagcgcgg
     4681 cgggtgtggt ggttacgcgc agcgtgaccg ctacacttgc cagcgcctta gcgcccgctc
     4741 ctttcgcttt cttcccttcc tttctcgcca cgttcgccgg ctttccccgt caagctctaa
     4801 atcgggggct ccctttaggg ttccgattta gtgctttacg gcacctcgac cccaaaaaac
     4861 ttgatttggg tgatggttca cgtagtgggc catcgccctg atagacggtt tttcgccctt
     4921 tgacgttgga gtccacgttc tttaatagtg gactcttgtt ccaaactgga acaacactca
     4981 actctatctc gggctattct tttgatttat aagggatttt gccgatttcg gtctattggt
     5041 taaaaaatga gctgatttaa caaaaattta acgcgaattt taacaaaata ttaacgttta
     5101 caattttatg gtgcactctc agtacaatct gctctgatgc cgcatagtta agccagcccc
     5161 gacacccgcc aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt
     5221 acagacaagc tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac
     5281 cgaaacgcgc gagacgaaag ggcctcgtga tacgcctatt tttataggtt aatgtcatga
     5341 taataatggt ttcttagacg tcaggtggca cttttcgggg aaatgtgcgc ggaaccccta
     5401 tttgtttatt tttctaaata cattcaaata tgtatccgct catgagacaa taaccctgat
     5461 aaatgcttca ataatattga aaaaggaaga gtatgagtat tcaacatttc cgtgtcgccc
     5521 ttattccctt ttttgcggca ttttgccttc ctgtttttgc tcacccagaa acgctggtga
     5581 aagtaaaaga tgctgaagat cagttgggtg cacgagtggg ttacatcgaa ctggatctca
     5641 acagcggtaa gatccttgag agttttcgcc ccgaagaacg ttttccaatg atgagcactt
     5701 ttaaagttct gctatgtggc gcggtattat cccgtattga cgccgggcaa gagcaactcg
     5761 gtcgccgcat acactattct cagaatgact tggttgagta ctcaccagtc acagaaaagc
     5821 atcttacgga tggcatgaca gtaagagaat tatgcagtgc tgccataacc atgagtgata
     5881 acactgcggc caacttactt ctgacaacga tcggaggacc gaaggagcta accgcttttt
     5941 tgcacaacat gggggatcat gtaactcgcc ttgatcgttg ggaaccggag ctgaatgaag
     6001 ccataccaaa cgacgagcgt gacaccacga tgcctgtagc aatggcaaca acgttgcgca
     6061 aactattaac tggcgaacta cttactctag cttcccggca acaattaata gactggatgg
     6121 aggcggataa agttgcagga ccacttctgc gctcggccct tccggctggc tggtttattg
     6181 ctgataaatc tggagccggt gagcgtgggt ctcgcggtat cattgcagca ctggggccag
     6241 atggtaagcc ctcccgtatc gtagttatct acacgacggg gagtcaggca actatggatg
     6301 aacgaaatag acagatcgct gagataggtg cctcactgat taagcattgg taactgtcag
     6361 accaagttta ctcatatata ctttagattg atttaaaact tcatttttaa tttaaaagga
     6421 tctaggtgaa gatccttttt gataatctca tgaccaaaat cccttaacgt gagttttcgt
     6481 tccactgagc gtcagacccc gtagaaaaga tcaaaggatc ttcttgagat cctttttttc
     6541 tgcgcgtaat ctgctgcttg caaacaaaaa aaccaccgct accagcggtg gtttgtttgc
     6601 cggatcaaga gctaccaact ctttttccga aggtaactgg cttcagcaga gcgcagatac
     6661 caaatactgt tcttctagtg tagccgtagt taggccacca cttcaagaac tctgtagcac
     6721 cgcctacata cctcgctctg ctaatcctgt taccagtggc tgctgccagt ggcgataagt
     6781 cgtgtcttac cgggttggac tcaagacgat agttaccgga taaggcgcag cggtcgggct
     6841 gaacgggggg ttcgtgcaca cagcccagct tggagcgaac gacctacacc gaactgagat
     6901 acctacagcg tgagctatga gaaagcgcca cgcttcccga agggagaaag gcggacaggt
     6961 atccggtaag cggcagggtc ggaacaggag agcgcacgag ggagcttcca gggggaaacg
     7021 cctggtatct ttatagtcct gtcgggtttc gccacctctg acttgagcgt cgatttttgt
     7081 gatgctcgtc aggggggcgg agcctatgga aaaacgccag caacgcggcc tttttacggt
     7141 tcctggcctt ttgctggcct tttgctcaca tgt
//