买家导航
pcDNA3.1+/N-GST(Thrombin)载体质粒基本信息
pcDNA3.1+/N-GST(Thrombin)质粒图谱载体图谱和pcDNA3.1+/N-GST(Thrombin)载体序列质粒序列多克隆位点信息
载体图谱.png)
pcDNA3.1+/N-GST(Thrombin)质粒载体简介
pcDNA3.1+/N-GST(Thrombin)质粒序列载体序列
LOCUS pcDNA3.1+ N-GST(Thrombin) 6080 bp ds-DNA circular SYN 21-MAY-2015
DEFINITION Mammalian vector with a CMV promoter for expressing proteins with an
N-terminal and thrombin-cleavable GST tag.
ACCESSION .
VERSION .
KEYWORDS pcDNA3.1+ N-GST(Thrombin)
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 6080)
AUTHORS GenScript
TITLE Direct Submission
JOURNAL Exported Tuesday, February 4, 2020 from SnapGene Viewer 3.1.4
http://www.snapgene.com
FEATURES Location/Qualifiers
source 1..6080
/organism="synthetic DNA construct"
/lab_host="Mammalian Cells"
/mol_type="other DNA"
enhancer 235..614
/note="CMV enhancer"
/note="human cytomegalovirus immediate early enhancer"
promoter 615..818
/note="CMV promoter"
/note="human cytomegalovirus (CMV) immediate early
promoter"
promoter 863..881
/note="T7 promoter"
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 923..1576
/codon_start=1
/product="glutathione S-transferase from Schistosoma
japonicum"
/note="GST"
/translation="MSPILGYWKIKGLVQPTRLLLEYLEEKYEEHLYERDEGDKWRNKK
FELGLEFPNLPYYIDGDVKLTQSMAIIRYIADKHNMLGGCPKERAEISMLEGAVLDIRY
GVSRIAYSKDFETLKVDFLSKLPEMLKMFEDRLCHKTYLNGDHVTHPDFMLYDALDVVL
YMDPMCLDAFPKLVCFKKRIEAIPQIDKYLKSSKYIAWPLQGWQATFGGGDHPPK"
CDS 1583..1600
/codon_start=1
/product="thrombin recognition and cleavage site"
/note="thrombin site"
/translation="LVPRGS"
misc_feature 1595..1651
/note="MCS"
/note="multiple cloning site"
polyA_signal 1677..1901
/note="bGH poly(A) signal"
/note="bovine growth hormone polyadenylation signal"
rep_origin 1947..2375
/direction=RIGHT
/note="f1 ori"
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 2389..2718
/note="SV40 promoter"
/note="SV40 enhancer and early promoter"
rep_origin 2569..2704
/note="SV40 ori"
/note="SV40 origin of replication"
CDS 2785..3579
/codon_start=1
/gene="aph(3')-II (or nptII)"
/product="aminoglycoside phosphotransferase from Tn5"
/note="NeoR/KanR"
/note="confers resistance to neomycin, kanamycin, and G418
(Geneticin(R))"
/translation="MIEQDGLHAGSPAAWVERLFGYDWAQQTIGCSDAAVFRLSAQGRP
VLFVKTDLSGALNELQDEAARLSWLATTGVPCAAVLDVVTEAGRDWLLLGEVPGQDLLS
SHLAPAEKVSIMADAMRRLHTLDPATCPFDHQAKHRIERARTRMEAGLVDQDDLDEEHQ
GLAPAELFARLKARMPDGEDLVVTHGDACLPNIMVENGRFSGFIDCGRLGVADRYQDIA
LATRDIAEELGGEWADRFLVLYGIAAPDSQRIAFYRLLDEFF"
polyA_signal 3753..3874
/note="SV40 poly(A) signal"
/note="SV40 polyadenylation signal"
rep_origin complement(4325..4913)
/direction=LEFT
/note="ori"
/note="high-copy-number ColE1/pMB1/pBR322/pUC origin of
replication"
CDS complement(5084..5944)
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/note="AmpR"
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
promoter complement(5945..6049)
/gene="bla"
/note="AmpR promoter"
ORIGIN
1 gacggatcgg gagatctccc gatcccctat ggtgcactct cagtacaatc tgctctgatg
61 ccgcatagtt aagccagtat ctgctccctg cttgtgtgtt ggaggtcgct gagtagtgcg
121 cgagcaaaat ttaagctaca acaaggcaag gcttgaccga caattgcatg aagaatctgc
181 ttagggttag gcgttttgcg ctgcttcgcg atgtacgggc cagatatacg cgttgacatt
241 gattattgac tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata
301 tggagttccg cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc
361 cccgcccatt gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc
421 attgacgtca atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt
481 atcatatgcc aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt
541 atgcccagta catgacctta tgggactttc ctacttggca gtacatctac gtattagtca
601 tcgctattac catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg
661 actcacgggg atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc
721 aaaatcaacg ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg
781 gtaggcgtgt acggtgggag gtctatataa gcagagctct ctggctaact agagaaccca
841 ctgcttactg gcttatcgaa attaatacga ctcactatag ggagacccaa gctggctagc
901 gtttaaactt aagcttgcca ccatgtcccc tatactaggt tattggaaaa ttaagggcct
961 tgtgcaaccc actcgacttc ttttggaata tcttgaagaa aaatatgaag agcatttgta
1021 tgagcgcgat gaaggtgata aatggcgaaa caaaaagttt gaattgggtt tggagtttcc
1081 caatcttcct tattatattg atggtgatgt taaattaaca cagtctatgg ccatcatacg
1141 ttatatagct gacaagcaca acatgttggg tggttgtcca aaagagcgtg cagagatttc
1201 aatgcttgaa ggagcggttt tggatattag atacggtgtt tcgagaattg catatagtaa
1261 agactttgaa actctcaaag ttgattttct tagcaagcta cctgaaatgc tgaaaatgtt
1321 cgaagatcgt ttatgtcata aaacatattt aaatggtgat catgtaaccc atcctgactt
1381 catgttgtat gacgctcttg atgttgtttt atacatggac ccaatgtgcc tggatgcgtt
1441 cccaaaatta gtttgtttta aaaaacgtat tgaagctatc ccacaaattg ataagtactt
1501 gaaatccagc aagtatatag catggccttt gcagggctgg caagccacgt ttggtggtgg
1561 cgaccatcct ccaaaatcgg atctggttcc gcgtggatcc gaattctgca gatatccagc
1621 acagtggcgg ccgctcgagt ctagagggcc cgtttaaacc cgctgatcag cctcgactgt
1681 gccttctagt tgccagccat ctgttgtttg cccctccccc gtgccttcct tgaccctgga
1741 aggtgccact cccactgtcc tttcctaata aaatgaggaa attgcatcgc attgtctgag
1801 taggtgtcat tctattctgg ggggtggggt ggggcaggac agcaaggggg aggattggga
1861 agacaatagc aggcatgctg gggatgcggt gggctctatg gcttctgagg cggaaagaac
1921 cagctggggc tctagggggt atccccacgc gccctgtagc ggcgcattaa gcgcggcggg
1981 tgtggtggtt acgcgcagcg tgaccgctac acttgccagc gccctagcgc ccgctccttt
2041 cgctttcttc ccttcctttc tcgccacgtt cgccggcttt ccccgtcaag ctctaaatcg
2101 ggggctccct ttagggttcc gatttagtgc tttacggcac ctcgacccca aaaaacttga
2161 ttagggtgat ggttcacgta gtgggccatc gccctgatag acggtttttc gccctttgac
2221 gttggagtcc acgttcttta atagtggact cttgttccaa actggaacaa cactcaaccc
2281 tatctcggtc tattcttttg atttataagg gattttgccg atttcggcct attggttaaa
2341 aaatgagctg atttaacaaa aatttaacgc gaattaattc tgtggaatgt gtgtcagtta
2401 gggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
2461 tagtcagcaa ccaggtgtgg aaagtcccca ggctccccag caggcagaag tatgcaaagc
2521 atgcatctca attagtcagc aaccatagtc ccgcccctaa ctccgcccat cccgccccta
2581 actccgccca gttccgccca ttctccgccc catggctgac taattttttt tatttatgca
2641 gaggccgagg ccgcctctgc ctctgagcta ttccagaagt agtgaggagg cttttttgga
2701 ggcctaggct tttgcaaaaa gctcccggga gcttgtatat ccattttcgg atctgatcaa
2761 gagacaggat gaggatcgtt tcgcatgatt gaacaagatg gattgcacgc aggttctccg
2821 gccgcttggg tggagaggct attcggctat gactgggcac aacagacaat cggctgctct
2881 gatgccgccg tgttccggct gtcagcgcag gggcgcccgg ttctttttgt caagaccgac
2941 ctgtccggtg ccctgaatga actgcaggac gaggcagcgc ggctatcgtg gctggccacg
3001 acgggcgttc cttgcgcagc tgtgctcgac gttgtcactg aagcgggaag ggactggctg
3061 ctattgggcg aagtgccggg gcaggatctc ctgtcatctc accttgctcc tgccgagaaa
3121 gtatccatca tggctgatgc aatgcggcgg ctgcatacgc ttgatccggc tacctgccca
3181 ttcgaccacc aagcgaaaca tcgcatcgag cgagcacgta ctcggatgga agccggtctt
3241 gtcgatcagg atgatctgga cgaagagcat caggggctcg cgccagccga actgttcgcc
3301 aggctcaagg cgcgcatgcc cgacggcgag gatctcgtcg tgacccatgg cgatgcctgc
3361 ttgccgaata tcatggtgga aaatggccgc ttttctggat tcatcgactg tggccggctg
3421 ggtgtggcgg accgctatca ggacatagcg ttggctaccc gtgatattgc tgaagagctt
3481 ggcggcgaat gggctgaccg cttcctcgtg ctttacggta tcgccgctcc cgattcgcag
3541 cgcatcgcct tctatcgcct tcttgacgag ttcttctgag cgggactctg gggttcgaaa
3601 tgaccgacca agcgacgccc aacctgccat cacgagattt cgattccacc gccgccttct
3661 atgaaaggtt gggcttcgga atcgttttcc gggacgccgg ctggatgatc ctccagcgcg
3721 gggatctcat gctggagttc ttcgcccacc ccaacttgtt tattgcagct tataatggtt
3781 acaaataaag caatagcatc acaaatttca caaataaagc atttttttca ctgcattcta
3841 gttgtggttt gtccaaactc atcaatgtat cttatcatgt ctgtataccg tcgacctcta
3901 gctagagctt ggcgtaatca tggtcatagc tgtttcctgt gtgaaattgt tatccgctca
3961 caattccaca caacatacga gccggaagca taaagtgtaa agcctggggt gcctaatgag
4021 tgagctaact cacattaatt gcgttgcgct cactgcccgc tttccagtcg ggaaacctgt
4081 cgtgccagct gcattaatga atcggccaac gcgcggggag aggcggtttg cgtattgggc
4141 gctcttccgc ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg
4201 tatcagctca ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa
4261 agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg
4321 cgtttttcca taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga
4381 ggtggcgaaa cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg
4441 tgcgctctcc tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg
4501 gaagcgtggc gctttctcat agctcacgct gtaggtatct cagttcggtg taggtcgttc
4561 gctccaagct gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg
4621 gtaactatcg tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca
4681 ctggtaacag gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt
4741 ggcctaacta cggctacact agaagaacag tatttggtat ctgcgctctg ctgaagccag
4801 ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg
4861 gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc
4921 ctttgatctt ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt
4981 tggtcatgag attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt
5041 ttaaatcaat ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca
5101 gtgaggcacc tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg
5161 tcgtgtagat aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac
5221 cgcgagaccc acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg
5281 ccgagcgcag aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc
5341 gggaagctag agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta
5401 caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac
5461 gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc
5521 ctccgatcgt tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac
5581 tgcataattc tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact
5641 caaccaagtc attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa
5701 tacgggataa taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt
5761 cttcggggcg aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca
5821 ctcgtgcacc caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa
5881 aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac
5941 tcatactctt cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg
6001 gatacatatt tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc
6061 gaaaagtgcc acctgacgtc
//
pcDNA3.1+/N-GST(Thrombin)载体图谱质粒图谱pdf版和pcDNA3.1+/N-GST(Thrombin)载体序列质粒序列等相关资料下载
pcDNA3.1+/N-GST(Thrombin)质粒载体应用举例
- 上一篇：pcDNA3.1+N-MYC
- 下一篇：pcDNA3.1+/N-GST(TEV)
