| 出品公司: | -- |
|---|---|
| 载体名称: | pRSETB-TagBFP |
| 质粒类型: | 大肠杆菌蓝色荧光表达质粒 |
| 高拷贝/低拷贝: | -- |
| 克隆方法: | -- |
| 启动子: | T7 |
| 载体大小: | 3553 bp |
| 5' 测序引物及序列: | T7 |
| 3' 测序引物及序列: | T7-ter |
| 载体标签: | -- |
| 载体抗性: | 氨苄青霉素 |
| 筛选标记: | TagBF |
| 克隆菌株: | DH5α 等 |
| 宿主细胞（系）: | -- |
| 备注: | -- |
| 产品目录号: | -- |
| 稳定性: | -- |
| 组成型/诱导型: | -- |
| 病毒/非病毒: | -- |
买家导航
pRSETB-TagBFP载体 pRSETBTagBFP质粒基本信息
pRSETB-TagBFP质粒图谱 pRSETBTagBFP载体图谱和pRSETB-TagBFP载体序列 pRSETBTagBFP质粒序列多克隆位点信息
pRSETB-TagBFP质粒 pRSETBTagBFP载体简介
pRSETB-TagBFP质粒序列 pRSETBTagBFP载体序列
LOCUS pRSETB-TagBFP 3553 bp ds-DNA circular SYN 29-JAN-2019
DEFINITION synthetic circular DNA
SOURCE synthetic DNA construct
ORGANISM synthetic DNA construct
REFERENCE 1 (bases 1 to 3553)
AUTHORS .
TITLE Direct Submission
FEATURES Location/Qualifiers
source 1..3553
/organism="synthetic DNA construct"
/mol_type="other DNA"
promoter 20..38
/label=T7 promoter
/note="promoter for bacteriophage T7 RNA polymerase"
CDS 112..129
/codon_start=1
/product="6xHis affinity tag"
/label=6xHis
/translation="HHHHHH"
CDS 133..165
/codon_start=1
/product="leader peptide from bacteriophage T7 gene 10"
/label=T7 tag (gene 10 leader)
/note="promotes efficient translation in E. coli"
/translation="MASMTGGQQMG"
CDS 169..192
/codon_start=1
/product="Xpress(TM) epitope tag, including an enterokinase
recognition and cleavage site"
/label=Xpress tag
/translation="DLYDDDDK"
CDS 199..900
/codon_start=1
/label=TagBFP
/translation="MSELIKENMHMKLYMEGTVDNHHFKCTSEGEGKPYEGTQTMRIKV
VEGGPLPFAFDILATSFLYGSKTFIDHTQGIPDFFKQSFPEGFTWERVTTYEDGGVLTA
TQDTSLQDGCLIYNVKIRGVNFTSNGPVMQKKTLGWEAFTETLYPADGGLEGRNDMALK
LVGGSHLIANIKTTYRSKKPAKNLKMPGVYYVDYRLERIKEANNETYVEQHEVAVARYC
DLPSKLGHKLN"
terminator 968..1015
/label=T7 terminator
/note="transcription terminator for bacteriophage T7 RNA
polymerase"
rep_origin 1112..1567
/direction=RIGHT
/label=f1 ori
/note="f1 bacteriophage origin of replication; arrow
indicates direction of (+) strand synthesis"
promoter 1593..1697
/gene="bla"
/label=AmpR promoter
CDS 1698..2558
/codon_start=1
/gene="bla"
/product="beta-lactamase"
/label=AmpR
/note="confers resistance to ampicillin, carbenicillin, and
related antibiotics"
/translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
LIKHW"
rep_origin 2729..3317
/direction=RIGHT
/label=ori
/note="high-copy-number colE1/pMB1/pBR322/pUC origin of
replication"
BASE COUNT 901 a 909 c 899 g 843 t
ORIGIN
1 gatctcgatc ccgcgaaatt aatacgactc actataggga gaccacaacg gtttccctct
61 agaaataatt ttgtttaact ttaagaagga gatatacata tgcggggttc tcatcatcat
121 catcatcatg gtatggctag catgactggt ggacagcaaa tgggtcggga tctgtacgac
181 gatgacgata aggatccgat gagcgagctg attaaggaga acatgcacat gaagctgtac
241 atggagggca ccgtggacaa ccatcacttc aagtgcacat ccgagggcga aggcaagccc
301 tacgagggca cccagaccat gagaatcaag gtggtcgagg gcggccctct ccccttcgcc
361 ttcgacatcc tggctactag cttcctctac ggcagcaaga ccttcatcga ccacacccag
421 ggcatccccg acttcttcaa gcagtccttc cctgagggct tcacatggga gagagtcacc
481 acatacgaag acgggggcgt gctgaccgct acccaggaca ccagcctcca ggacggctgc
541 ctcatctaca acgtcaagat cagaggggtg aacttcacat ccaacggccc tgtgatgcag
601 aagaaaacac tcggctggga ggccttcacc gagacgctgt accccgctga cggcggcctg
661 gaaggcagaa acgacatggc cctgaagctc gtgggcggga gccatctgat cgcaaacatc
721 aagaccacat atagatccaa gaaacccgct aagaacctca agatgcctgg cgtctactat
781 gtggactaca gactggaaag aatcaaggag gccaacaacg agacctacgt cgagcagcac
841 gaggtggcag tggccagata ctgcgacctc cctagcaaac tggggcacaa gctcaattaa
901 gcttgatccg gctgctaaca aagcccgaaa ggaagctgag ttggctgctg ccaccgctga
961 gcaataacta gcataacccc ttggggcctc taaacgggtc ttgaggggtt ttttgctgaa
1021 aggaggaact atatccggat ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc
1081 caacagttgc gcagcctgaa tggcgaatgg gacgcgccct gtagcggcgc attaagcgcg
1141 gcgggtgtgg tggttacgcg cagcgtgacc gctacacttg ccagcgccct agcgcccgct
1201 cctttcgctt tcttcccttc ctttctcgcc acgttcgccg gctttccccg tcaagctcta
1261 aatcgggggc tccctttagg gttccgattt agtgctttac ggcacctcga ccccaaaaaa
1321 cttgattagg gtgatggttc acgtagtggg ccatcgccct gatagacggt ttttcgccct
1381 ttgacgttgg agtccacgtt ctttaatagt ggactcttgt tccaaactgg aacaacactc
1441 aaccctatct cggtctattc ttttgattta taagggattt tgccgatttc ggcctattgg
1501 ttaaaaaatg agctgattta acaaaaattt aacgcgaatt ttaacaaaat attaacgctt
1561 acaatttagg tggcactttt cggggaaatg tgcgcggaac ccctatttgt ttatttttct
1621 aaatacattc aaatatgtat ccgctcatga gacaataacc ctgataaatg cttcaataat
1681 attgaaaaag gaagagtatg agtattcaac atttccgtgt cgcccttatt cccttttttg
1741 cggcattttg ccttcctgtt tttgctcacc cagaaacgct ggtgaaagta aaagatgctg
1801 aagatcagtt gggtgcacga gtgggttaca tcgaactgga tctcaacagc ggtaagatcc
1861 ttgagagttt tcgccccgaa gaacgttttc caatgatgag cacttttaaa gttctgctat
1921 gtggcgcggt attatcccgt attgacgccg ggcaagagca actcggtcgc cgcatacact
1981 attctcagaa tgacttggtt gagtactcac cagtcacaga aaagcatctt acggatggca
2041 tgacagtaag agaattatgc agtgctgcca taaccatgag tgataacact gcggccaact
2101 tacttctgac aacgatcgga ggaccgaagg agctaaccgc ttttttgcac aacatggggg
2161 atcatgtaac tcgccttgat cgttgggaac cggagctgaa tgaagccata ccaaacgacg
2221 agcgtgacac cacgatgcct gtagcaatgg caacaacgtt gcgcaaacta ttaactggcg
2281 aactacttac tctagcttcc cggcaacaat taatagactg gatggaggcg gataaagttg
2341 caggaccact tctgcgctcg gcccttccgg ctggctggtt tattgctgat aaatctggag
2401 ccggtgagcg tgggtctcgc ggtatcattg cagcactggg gccagatggt aagccctccc
2461 gtatcgtagt tatctacacg acggggagtc aggcaactat ggatgaacga aatagacaga
2521 tcgctgagat aggtgcctca ctgattaagc attggtaact gtcagaccaa gtttactcat
2581 atatacttta gattgattta aaacttcatt tttaatttaa aaggatctag gtgaagatcc
2641 tttttgataa tctcatgacc aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag
2701 accccgtaga aaagatcaaa ggatcttctt gagatccttt ttttctgcgc gtaatctgct
2761 gcttgcaaac aaaaaaacca ccgctaccag cggtggtttg tttgccggat caagagctac
2821 caactctttt tccgaaggta actggcttca gcagagcgca gataccaaat actgttcttc
2881 tagtgtagcc gtagttaggc caccacttca agaactctgt agcaccgcct acatacctcg
2941 ctctgctaat cctgttacca gtggctgctg ccagtggcga taagtcgtgt cttaccgggt
3001 tggactcaag acgatagtta ccggataagg cgcagcggtc gggctgaacg gggggttcgt
3061 gcacacagcc cagcttggag cgaacgacct acaccgaact gagataccta cagcgtgagc
3121 tatgagaaag cgccacgctt cccgaaggga gaaaggcgga caggtatccg gtaagcggca
3181 gggtcggaac aggagagcgc acgagggagc ttccaggggg aaacgcctgg tatctttata
3241 gtcctgtcgg gtttcgccac ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg
3301 ggcggagcct atggaaaaac gccagcaacg cggccttttt acggttcctg gccttttgct
3361 ggccttttgc tcacatgttc tttcctgcgt tatcccctga ttctgtggat aaccgtatta
3421 ccgcctttga gtgagctgat accgctcgcc gcagccgaac gaccgagcgc agcgagtcag
3481 tgagcgagga agcggaagag cgcccaatac gcaaaccgcc tctccccgcg cgttggccga
3541 ttcattaatg cag
//
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