pMIG-FLAG-MLL-AF9

LOCUS        pMIG-FLAG-MLL-AF9      11089 bp    DNA     circular SYN 17-JUL-2025
DEFINITION  Retroviral construct to express FLAG-tagged MLL-AF9 fusion gene, 
            followed by IRES-GFP.
ACCESSION   .
VERSION     .
KEYWORDS    .
SOURCE      synthetic DNA construct
  ORGANISM  synthetic DNA construct
REFERENCE   1  (bases 1 to 11089)
  AUTHORS   Saito Y, Chapple RH, Lin A, Kitano A, Nakada D
  TITLE     AMPK Protects Leukemia-Initiating Cells in Myeloid Leukemias from 
            Metabolic Stress in the Bone Marrow.
  JOURNAL   Cell Stem Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 
            10.1016/j.stem.2015.08.019.
  PUBMED    26440282
REFERENCE   2  (bases 1 to 11089)
  AUTHORS   .
  TITLE     Direct Submission
  JOURNAL   Exported Jul 17, 2025 from SnapGene Server 8.0.1
            https://www.biofeng.com
COMMENT     SGRef: number: 1; type: "Journal Article"; journalName: "Cell Stem 
            Cell. 2015 Sep 29. pii: S1934-5909(15)00374-4. doi: 
            10.1016/j.stem.2015.08.019."
COMMENT     Sequence Label: pMIG-FLAG-MLL-AF9
FEATURES             Location/Qualifiers
     source          1..11089
                     /mol_type="other DNA"
                     /organism="synthetic DNA construct"
     primer_bind     24..43
                     /label=pRS-marker
                     /note="pRS vectors, use to sequence yeast selectable 
                     marker"
     primer_bind     237..259
                     /label=M13/pUC Forward
                     /note="In lacZ gene"
     primer_bind     387..406
                     /label=pBRrevBam
                     /note="pBR322 vectors, tet region, downstream of BamHI, 
                     reverse primer"
     LTR             606..1122
                     /label=5' LTR
                     /note="5' long terminal repeat from murine embryonic stem 
                     cell virus"
     misc_feature    1186..1527
                     /label=MESV Psi
                     /note="packaging signal of murine embryonic stem cell 
                     virus"
     CDS             1594..2010
                     /label=gag (truncated)
                     /note="truncated Moloney murine leukemia virus (MMLV) gag 
                     gene lacking the start codon"
     primer_bind     1940..1962
                     /label=pLXSN 5'
                     /note="Murine stem cell virus, forward primer. Also called 
                     MSCV"
     primer_bind     1978..1994
                     /label=pBABE 5'
                     /note="Psi packaging signal, forward primer for pBABE 
                     vectors"
     CDS             2041..2064
                     /codon_start=1
                     /product="FLAG(R) epitope tag, followed by an enterokinase 
                     cleavage site"
                     /label=FLAG
                     /translation="DYKDDDDK"
     misc_feature    join(6632..7207,7208..7210,7211..7219)
                     /label=IRES2
                     /note="internal ribosome entry site (IRES) of the 
                     encephalomyocarditis virus (EMCV)"
     primer_bind     complement(6800..6817)
                     /label=IRES reverse
                     /note="IRES internal ribosome entry site, reverse primer. 
                     Also called pCDH-rev"
     primer_bind     7027..7046
                     /label=IRES-F
                     /note="IRES internal ribosome entry site, forward primer"
     CDS             join(7220..7222,7223..7225,7226..7939)
                     /codon_start=1
                     /product="the original enhanced GFP (Yang et al., 1996)"
                     /label=EGFP
                     /note="mammalian codon-optimized"
                     /translation="MVSKGEELFTGVVPILVELDGDVNGHKFSVSGEGEGDATYGKLTL
                     KFICTTGKLPVPWPTLVTTLTYGVQCFSRYPDHMKQHDFFKSAMPEGYVQERTIFFKDD
                     GNYKTRAEVKFEGDTLVNRIELKGIDFKEDGNILGHKLEYNYNSHNVYIMADKQKNGIK
                     ANFKIRHNIEDGSVQLADHYQQNTPIGDGPVLLPDNHYLSTQSALSKDPNEKRDHMVLL
                     EFVTAAGITLGMDELYK"
     primer_bind     complement(7265..7286)
                     /label=EGFP-N
                     /note="EGFP, reverse primer"
     primer_bind     complement(7526..7545)
                     /label=EXFP-R
                     /note="For distinguishing EGFP variants, reverse primer"
     primer_bind     7873..7894
                     /label=EGFP-C
                     /note="EGFP, forward primer"
     LTR             8089..8603
                     /label=3' LTR
                     /note="3' long terminal repeat from murine embryonic stem 
                     cell virus"
     protein_bind    8765..8781
                     /label=lac operator
                     /bound_moiety="lac repressor encoded by lacI"
                     /note="The lac repressor binds to the lac operator to 
                     inhibit transcription in E. coli. This inhibition can be 
                     relieved by adding lactose or 
                     isopropyl-beta-D-thiogalactopyranoside (IPTG)."
     promoter        complement(join(8789..8795,8796..8813,8814..8819))
                     /label=lac promoter
                     /note="promoter for the E. coli lac operon"
     protein_bind    8834..8855
                     /label=CAP binding site
                     /bound_moiety="E. coli catabolite activator protein"
                     /note="CAP binding activates transcription in the presence 
                     of cAMP."
     primer_bind     complement(8972..8989)
                     /label=L4440
                     /note="L4440 vector, forward primer"
     rep_origin      complement(9143..9731)
                     /direction=LEFT
                     /label=ori
                     /note="high-copy-number ColE1/pMB1/pBR322/pUC origin of 
                     replication"
     primer_bind     complement(9223..9242)
                     /label=pBR322ori-F
                     /note="pBR322 origin, forward primer"
     CDS             complement(join(9902..10693,10694..10762))
                     /codon_start=1
                     /gene="bla"
                     /product="beta-lactamase"
                     /label=AmpR
                     /note="confers resistance to ampicillin, carbenicillin, and
                     related antibiotics"
                     /translation="MSIQHFRVALIPFFAAFCLPVFAHPETLVKVKDAEDQLGARVGYI
                     ELDLNSGKILESFRPEERFPMMSTFKVLLCGAVLSRIDAGQEQLGRRIHYSQNDLVEYS
                     PVTEKHLTDGMTVRELCSAAITMSDNTAANLLLTTIGGPKELTAFLHNMGDHVTRLDRW
                     EPELNEAIPNDERDTTMPVAMATTLRKLLTGELLTLASRQQLIDWMEADKVAGPLLRSA
                     LPAGWFIADKSGAGERGSRGIIAALGPDGKPSRIVVIYTTGSQATMDERNRQIAEIGAS
                     LIKHW"
     primer_bind     10525..10544
                     /label=Amp-R
                     /note="Ampicillin resistance gene, reverse primer"
     promoter        complement(10763..10867)
                     /gene="bla"
                     /label=AmpR promoter
     primer_bind     10935..10953
                     /label=pBRforEco
                     /note="pBR322 vectors, upsteam of EcoRI site, forward 
                     primer"
     primer_bind     complement(10991..11013)
                     /label=pGEX 3'
                     /note="pGEX vectors, reverse primer"
ORIGIN
        1 gtgtcggggc tggcttaact atgcggcatc agagcagatt gtactgagag tgcaccatat
       61 gcggtgtgaa ataccgcaca gatgcgtaag gagaaaatac cgcatcaggc gccattcgcc
      121 attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca
      181 gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag ggttttccca
      241 gtcacgacgt tgtaaaacga cggcgcaagg aatggtgcat gcaaggagat ggcgcccaac
      301 agtcccccgg ccacggggcc tgccaccata cccacgccga aacaagcgct catgagcccg
      361 aagtggcgag cccgatcttc cccatcggtg atgtcggcga tataggcgcc agcaaccgca
      421 cctgtggcgc cggtgatgcc ggccacgatg cgtccggcgt agaggcgatt agtccaattt
      481 gttaaagaca ggatatcagt ggtccaggct ctagttttga ctcaacaata tcaccagctg
      541 aagcctatag agtacgagcc atagataaaa taaaagattt tatttagtct ccagaaaaag
      601 gggggaatga aagaccccac ctgtaggttt ggcaagctag cttaagtaac gccattttgc
      661 aaggcatgga aaatacataa ctgagaatag agaagttcag atcaaggtta ggaacagaga
      721 gacagcagaa tatgggccaa acaggatatc tgtggtaagc agttcctgcc ccggctcagg
      781 gccaagaaca gatggtcccc agatgcggtc ccgccctcag cagtttctag agaaccatca
      841 gatgtttcca gggtgcccca aggacctgaa atgaccctgt gccttatttg aactaaccaa
      901 tcagttcgct tctcgcttct gttcgcgcgc ttctgctccc cgagctcaat aaaagagccc
      961 acaacccctc actcggcgcg ccagtcctcc gatagactgc gtcgcccggg tacccgtatt
     1021 cccaataaag cctcttgctg tttgcatccg aatcgtggac tcgctgatcc ttgggagggt
     1081 ctcctcagat tgattgactg cccacctcgg gggtctttca tttggaggtt ccaccgagat
     1141 ttggagaccc ctgcccaggg accaccgacc cccccgccgg gaggtaagct ggccagcggt
     1201 cgtttcgtgt ctgtctctgt ctttgtgcgt gtttgtgccg gcatctaatg tttgcgcctg
     1261 cgtctgtact agttagctaa ctagctctgt atctggcgga cccgtggtgg aactgacgag
     1321 ttctgaacac ccggccgcaa ccctgggaga cgtcccaggg actttggggg ccgtttttgt
     1381 ggcccgacct gaggaaggga gtcgatgtgg aatccgaccc cgtcaggata tgtggttctg
     1441 gtaggagacg agaacctaaa acagttcccg cctccgtctg aatttttgct ttcggtttgg
     1501 aaccgaagcc gcgcgtcttg tctgctgcag cgctgcagca tcgttctgtg ttgtctctgt
     1561 ctgactgtgt ttctgtattt gtctgaaaat tagggccaga ctgttaccac tcccttaagt
     1621 ttgaccttag gtcactggaa agatgtcgag cggatcgctc acaaccagtc ggtagatgtc
     1681 aagaagagac gttgggttac cttctgctct gcagaatggc caacctttaa cgtcggatgg
     1741 ccgcgagacg gcacctttaa ccgagacctc atcacccagg ttaagatcaa ggtcttttca
     1801 cctggcccgc atggacaccc agaccaggtc ccctacatcg tgacctggga agccttggct
     1861 tttgaccccc ctccctgggt caagcccttt gtacacccta agcctccgcc tcctcttcct
     1921 ccatccgccc cgtctctccc ccttgaacct cctcgttcga ccccgcctcg atcctccctt
     1981 tatccagccc tcactccttc tctaggcgcc ggaattagat ctctcgaggt taattccatg
     2041 gactacaagg acgacgatga caagggcaat tccgcgaaca tggcgcacag ctgtcggtgg
     2101 cgcttccccg cccgacccgg gaccaccggg ggcggcggcg gcggggggcg ccggggccta
     2161 gggggcgccc cgcggcaacg cgtcccggcc ctgctgcttc cccccgggcc cccggtcggc
     2221 ggtggcggcc ccggggcgcc cccctccccc ccggctgtgg cggccgcggc ggcggcggcg
     2281 ggaagcagcg gggctggggt tccaggggga gcggccgccg cctcagcagc ctcctcgtcg
     2341 tccgcctcgt cttcgtcttc gtcatcgtcc tcagcctctt cagggccggc cctgctccgg
     2401 gtgggcccgg gcttcgacgc ggcgctgcag gtctcggccg ccatcggcac caacctgcgc
     2461 cggttccggg ccgtgtttgg ggagagcggc gggggaggcg gcagcggaga ggatgagcaa
     2521 ttcttaggtt ttggctcaga tgaagaagtc agagtgcgaa gtcccacaag gtctccttca
     2581 gttaaaacta gtcctcgaaa acctcgtggg agacctagaa gtggctctga ccgaaattca
     2641 gctatcctct cagatccatc tgtgttttcc cctctaaata aatcagagac caaatctgga
     2701 gataagatca agaagaaaga ttctaaaagt atagaaaaga agagaggaag acctcccacc
     2761 ttccctggag taaaaatcaa aataacacat ggaaaggaca tttcagagtt accaaaggga
     2821 aacaaagaag atagcctgaa aaaaattaaa aggacacctt ctgctacgtt tcagcaagcc
     2881 acaaagatta aaaaattaag agcaggtaaa ctctctcctc tcaagtctaa gtttaagaca
     2941 gggaagcttc aaataggaag gaagggggta caaattgtac gacggagagg aaggcctcca
     3001 tcaacagaaa ggataaagac cccttcgggt ctcctcatta attctgaact ggaaaagccc
     3061 cagaaagtcc ggaaagacaa ggaaggaaca cctccactta caaaagaaga taagacagtt
     3121 gtcagacaaa gccctcgaag gattaagcca gttaggatta ttccttcttc aaaaaggaca
     3181 gatgcaacca ttgctaagca actcttacag agggcaaaaa agggggctca aaagaaaatt
     3241 gaaaaagaag cagctcagct gcagggaaga aaggtgaaga cacaggtcaa aaatattcga
     3301 cagttcatca tgcctgttgt cagtgctatc tcctcgcgga tcattaagac ccctcggcgg
     3361 tttatagagg atgaggatta tgaccctcca attaaaattg cccgattaga gtctacaccg
     3421 aatagtagat tcagtgcccc gtcctgtgga tcttctgaaa aatcaagtgc agcttctcag
     3481 cactcctctc aaatgtcttc agactcctct cgatctagta gccccagtgt tgatacctcc
     3541 acagactctc aggcttctga ggagattcag gtacttcctg aggagcggag cgatacccct
     3601 gaagttcatc ctccactgcc catttcccag tccccagaaa atgagagtaa tgataggaga
     3661 agcagaaggt attcagtgtc ggagagaagt tttggatcta gaacgacgaa aaaattatca
     3721 actctacaaa gtgcccccca gcagcagacc tcctcgtctc cacctccacc tctgctgact
     3781 ccaccgccac cactgcagcc agcctccagt atctctgacc acacaccttg gcttatgcct
     3841 ccaacaatcc ccttagcatc accatttttg cctgcttcca ctgctcctat gcaagggaag
     3901 cgaaaatcta ttttgcgaga accgacattt aggtggactt ctttaaagca ttctaggtca
     3961 gagccacaat acttttcctc agcaaagtat gccaaagaag gtcttattcg caaaccaata
     4021 tttgataatt tccgaccccc tccactaact cccgaggacg ttggctttgc atctggtttt
     4081 tctgcatctg gtaccgctgc ttcagcccga ttgttttcgc cactccattc tggaacaagg
     4141 tttgatatgc acaaaaggag ccctcttctg agagctccaa gatttactcc aagtgaggct
     4201 cactctagaa tatttgagtc tgtaaccttg cctagtaatc gaacttctgc tggaacatct
     4261 tcttcaggag tatccaatag aaaaaggaaa agaaaagtgt ttagtcctat tcgatctgaa
     4321 ccaagatctc cttctcactc catgaggaca agaagtggaa ggcttagtag ttctgagctc
     4381 tcacctctca cccccccgtc ttctgtctct tcctcgttaa gcatttctgt tagtcctctt
     4441 gccactagtg ccttaaaccc aacttttact tttccttctc attccctgac tcagtctggg
     4501 gaatctgcag agaaaaatca gagaccaagg aagcagacta gtgctccggc agagccattt
     4561 tcatcaagta gtcctactcc tctcttccct tggtttaccc caggctctca gactgaaaga
     4621 gggagaaata aagacaaggc ccccgaggag ctgtccaaag atcgagatgc tgacaagagc
     4681 gtggagaagg acaagagtag agagagagac cgggagagag aaaaggagaa taagcgggag
     4741 tcaaggaaag agaaaaggaa aaagggatca gaaattcaga gtagttctgc tttgtatcct
     4801 gtgggtaggg tttccaaaga gaaggttgtt ggtgaagatg ttgccacttc atcttctgcc
     4861 aaaaaagcaa cagggcggaa gaagtcttca tcacatgatt ctgggactga tattacttct
     4921 gtgactcttg gggatacaac agctgtcaaa accaaaatac ttataaagaa agggagagga
     4981 aatctggaaa aaaccaactt ggacctcggc ccaactgccc catccctgga gaaggagaaa
     5041 accctctgcc tttccactcc ttcatctagc actgttaaac attccacttc ctccataggc
     5101 tccatgttgg ctcaggcaga caagcttcca atgactgaca agagggttgc cagcctccta
     5161 aaaaaggcca aagctcagct ctgcaagatt gagaagagta agagtcttaa acaaaccgac
     5221 cagcccaaag cacagggtca agaaagtgac tcatcagaga cctctgtgcg aggaccccgg
     5281 attaaacatg tctgcagaag agcagctgtt gcccttggcc gaaaacgagc tgtgtttcct
     5341 gatgacatgc ccaccctgag tgccttacca tgggaagaac gagaaaagat tttgtcttcc
     5401 atggggaatg atgacaagtc atcaattgct ggctcagaag atgctgaacc tcttgctcca
     5461 cccatcaaac caattaaacc tgtcactaga aacaaggcac cccaggaacc tccagtaaag
     5521 aaaggacgtc gatcgaggcg gtgtgggcag tgtcccggct gccaggtgcc tgaggactgt
     5581 ggtgtttgta ctaattgctt agataagccc aagtttggtg gtcgcaatat aaagaagcag
     5641 tgctgcaaga tgagaaaatg tcagaatcta caatggatgc cttccaaagc ctacctgcag
     5701 aagcaagcta aagctgtgaa aaagaaagag aaaaagtcta agaccagtga aaagaaagac
     5761 agcaaagaga gcagtgttgt gaagaacgtg gtggactcta gtcagaaacc taccccatca
     5821 gcaagagagg atcctgcccc aaagaaaagc agtagtgagc ctcctccacg aaagcccgtc
     5881 gaggaaaaga gtgaagaagg gaatgtctcg gcccctgggc ctgaatccaa acaggccacc
     5941 actccagctt ccaggaagtc aagcaagcag gtctcccagc cagcactggt catcccgcct
     6001 cagccaccta ctacaggacc gccaagaaaa gaagttccca aaaccactcc tagtgagccc
     6061 aagaaaaagc agcctccacc accagaatca ggtccagagc agagcaaaca gaaaaaagtg
     6121 gctccccgcc caagtatccc tgtaaaacaa aaaccaaaag aaaaggaaaa accacctccg
     6181 gtcaataagc aggagaatgc aggcactttg aacatcctca gcactctctc caatggcaat
     6241 agttctaagc aaaaaattcc agcagatggg tcgcgaagta tacacacgtg tcttgaagtg
     6301 aaaagtccaa taaagcaaag caaatcagat aagcaaataa agaatggtga atgtgacaag
     6361 gcatacctag atgaactggt agagcttcac agaaggttaa tgacattgag agaaagacac
     6421 attctgcagc agatcgtgaa ccttatagaa gaaactggac actttcatat cacaaacaca
     6481 acatttgatt ttgatctttg ctcgctggac aaaaccacag tccgtaaact acagagttac
     6541 ctggaaacat ctggaacatc ctgactcgag ggatccgggc cctctagatg cggccgcatg
     6601 catacgaatt ccgccccccc cccccccccc ccccctctcc ctcccccccc ccctaacgtt
     6661 actggccgaa gccgcttgga ataaggccgg tgtgcgtttg tctatatgtt attttccacc
     6721 atattgccgt cttttggcaa tgtgagggcc cggaaacctg gccctgtctt cttgacgagc
     6781 attcctaggg ctctttcccc tctcgccaaa ggaatgcaag gtctgttgaa tgtcgtgaag
     6841 gaagcagttc ctctggaagc ttcttgaaga caaacaacgt ctgtagcgac cctttgcagg
     6901 cagcggaacc ccccacctgg cgacaggtgc ctctgcggcc aaaagccacg tgtataagat
     6961 acacctgcaa aggcggcaca accccagtgc cacgttgtga gttggatagt tgtggaaaga
     7021 gtcaaatggc tctcctcaag cgtattcaac aaggggctga aggatgccca gaaggtaccc
     7081 cattgtatgg gatctgatct ggggcctcgg tgcacatgct ttacatgtgt ttagtcgagg
     7141 ttaaaaaaac gtctaggccc cccgaaccac ggggacgtgg ttttcctttg aaaaacacga
     7201 tgataatatg gccacaacca tggtgagcaa gggcgaggag ctgttcaccg gggtggtgcc
     7261 catcctggtc gagctggacg gcgacgtaaa cggccacaag ttcagcgtgt ctggcgaggg
     7321 cgagggcgat gccacctacg gcaagctgac cctgaagttc atctgcacca ccggcaagct
     7381 gcccgtgccc tggcccaccc tcgtgaccac cctgacctac ggcgtgcagt gcttcagccg
     7441 ctaccccgac cacatgaagc agcacgactt cttcaagtcc gccatgcccg aaggctacgt
     7501 ccaggagcgc accatcttct tcaaggacga cggcaactac aagacccgcg ccgaggtgaa
     7561 gttcgagggc gacaccctgg tgaaccgcat cgagctgaag ggcatcgact tcaaggagga
     7621 cggcaacatc ctggggcaca agctggagta caactacaac agccacaacg tctatatcat
     7681 ggccgacaag cagaagaacg gcatcaaggc gaacttcaag atccgccaca acatcgagga
     7741 cggcagcgtg cagctcgccg accactacca gcagaacacc cccatcggcg acggccccgt
     7801 gctgctgccc gacaaccact acctgagcac ccagtccgcc ctgagcaaag accccaacga
     7861 gaagcgcgat cacatggtcc tgctggagtt cgtgaccgcc gccgggatca ctctcggcat
     7921 ggacgagctg tacaagtaat gaattaatta agaattatca ccgcttctat tcagccagta
     7981 aggcctgtct ttaatggcct ccggcatgag acactttcta gagtcgacct gcagccaagc
     8041 ttatcgataa aataaaagat tttatttagt ctccagaaaa aggggggaat gaaagacccc
     8101 acctgtaggt ttggcaagct agcttaagta acgccatttt gcaaggcatg gaaaatacat
     8161 aactgagaat agagaagttc agatcaaggt taggaacaga gagacagcag aatatgggcc
     8221 aaacaggata tctgtggtaa gcagttcctg ccccggctca gggccaagaa cagatggtcc
     8281 ccagatgcgg tcccgccctc agcagtttct agagaaccat cagatgtttc cagggtgccc
     8341 caaggacctg aaatgaccct gtgccttatt tgaactaacc aatcagttcg cttctcgctt
     8401 ctgttcgcgc gcttctgctc cccgagctca ataaaagagc ccacaacccc tcactcggcg
     8461 cgccagtcct ccgatagact gcgtcgcccg ggtacccgtg tatccaataa accctcttgc
     8521 agttgcatcc gacttgtggt ctcgctgttc cttgggaggg tctcctctga gtgattgact
     8581 acccgtcagc gggggtcttt catgggtaac agtttcttga agttggagaa caacattctg
     8641 agggtaggag tcgaatatta agtaatcctg actcaattag ccactgtttt gaatccacat
     8701 actccaatac tcctgaaata gttcattatg gacagcgcag aaagagctgg ggagaattgt
     8761 gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     8821 cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     8881 tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     8941 gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     9001 ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     9061 caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     9121 aaaaggccgc gttgctggcg tttttccata ggctccgccc ccctgacgag catcacaaaa
     9181 atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     9241 cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     9301 ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     9361 gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     9421 accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     9481 cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     9541 cagagttctt gaagtggtgg cctaactacg gctacactag aagaacagta tttggtatct
     9601 gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     9661 aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     9721 aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     9781 actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     9841 taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     9901 gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     9961 tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
    10021 ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
    10081 accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
    10141 agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
    10201 acgttgttgc cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
    10261 tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
    10321 cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
    10381 tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
    10441 ctgtgactgg tgagtactca accaagtcat tctgagaata gtgtatgcgg cgaccgagtt
    10501 gctcttgccc ggcgtcaata cgggataata ccgcgccaca tagcagaact ttaaaagtgc
    10561 tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
    10621 ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
    10681 gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
    10741 cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
    10801 gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
    10861 ttccgcgcac atttccccga aaagtgccac ctgacgtcta agaaaccatt attatcatga
    10921 cattaaccta taaaaatagg cgtatcacga ggccctttcg tctcgcgcgt ttcggtgatg
    10981 acggtgaaaa cctctgacac atgcagctcc cggagacggt cacagcttgt ctgtaagcgg
    11041 atgccgggag cagacaagcc cgtcagggcg cgtcagcggg tgttggcgg
//